Article
Biochemistry & Molecular Biology
Annalisa Chiavaroli, Viviana di Giacomo, Barbara De Filippis, Amelia Cataldi, Claudio Ferrante, Letizia Giampietro
Summary: PPAR gamma agonists, such as GL516, have been shown to regulate diabetes, metabolic syndrome, and brain disorders. This study investigated the effects of GL516 on dopamine and serotonin levels, as well as the gene expressions of neuropeptides involved in feeding behavior and energy balance. The results demonstrated that GL516 reduced dopamine and serotonin turnover and stimulated the expression of orexigenic neuropeptides. These findings suggest that GL516 has potential therapeutic applications in neurodegenerative diseases associated with reduced food intake and catabolic pathways.
Article
Biochemistry & Molecular Biology
Katarzyna Szajko, Paulina Smyda-Dajmund, Jaroslaw Ciekot, Waldemar Marczewski, Dorota Soltys-Kalina
Summary: In this study, we investigated the relationship between plant phytotoxicity and glycoalkaloid content in potato leaf extracts. We found that potato progeny with high glycoalkaloid content showed phytotoxicity against mustard, while progeny with low glycoalkaloid content stimulated mustard growth. RNA sequencing analysis revealed that the upregulation of a flavonol synthase/flavonone 3-hydroxylase-like gene in low phytotoxicity progeny stimulated plant growth. These findings suggest that metabolic shifts and specific compositions of glycoalkaloids in potato leaf extracts may be responsible for different physiological responses in mustard.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Meimei Li, Boliao Li, Qi Yang, Yanying Li, Junxiang Wu, Xiangli Xu
Summary: In this study, we identified 50 neuropeptides including neuropeptide Y (NPY) of Mythimna separata using transcriptome sequencing. The spatial and temporal expression profile of NPY indicated its important role in feeding regulation and energy metabolism. Knockdown of NPY significantly inhibited food uptake and body weight, delayed developmental duration, and altered energy storage in M. separata larvae.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Swetha Kumar, Venkata Gayatri Dhara, Linda D. Orzolek, Haiping Hao, Abbie J. More, Eduardo Catchon Lau, Michael J. Betenbaugh
Summary: The study found that plant-based hydrolysates, such as cottonseed hydrolysate, can positively impact CHO cell growth and productivity by upregulating transcription and translation, and affecting pathways related to the cell cycle and metabolism.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Toxicology
Julia Buchmueller, Heike Sprenger, Johanna Ebmeyer, Josef Daniel Rasinger, Otto Creutzenberg, Dirk Schaudien, Jan G. Hengstler, Georgia Guenther, Albert Braeuning, Stefanie Hessel-Pras
Summary: This study investigated the gene expression changes in rat lungs and kidneys after exposure to six different PAs, highlighting inflammatory responses in lungs and cell-cycle regulation and DNA damage response in the liver. The results demonstrate tissue-specific molecular effects of PAs and provide valuable information on the early molecular effects in lung tissue after subacute exposure to PAs.
ARCHIVES OF TOXICOLOGY
(2021)
Review
Virology
Antonio Victor Campos Coelho, Rossella Gratton, Joao Paulo Britto de Melo, Jose Leandro Andrade-Santos, Rafael Lima Guimaraes, Sergio Crovella, Paola Maura Tricarico, Lucas Andre Cavalcanti Brandao
Summary: This study conducted a meta-analysis of RNA-Seq expression profiles in samples of HIV-1 infected CD4+ T cells, identifying 208 differentially expressed genes related to immune response, cell adhesion, cell migration, inflammation, apoptosis, and signaling pathways.
Article
Plant Sciences
Yi-Ju Chen, Michael A. Catto, Sudeep Pandey, Soraya Leal-Bertioli, Mark Abney, Brendan G. Hunt, Sudeep Bag, Albert Culbreath, Rajagopalbabu Srinivasan
Summary: Crossbreeding wild diploid species could introgress alleles that confer resistance to tomato spotted wilt orthotospovirus (TSWV) into cultivated peanut. Two diploids and their allotetraploids were identified with reduced TSWV infection. The number of differentially expressed genes (DEGs) in response to TSWV infection varied among the genotypes. A higher proportion of genes decreased in expression following infection in A. stenosperma and ValSten1, whereas a higher proportion of genes increased in expression following infection in A. valida. The number of defense-related DEGs was highest in A. valida.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Infectious Diseases
Miftahul Faridl, Karlina Mellyani, Karimatu Khoirunnisa, Popi Septiani, Ernawati Arifin Giri-Rachman, Husna Nugrahapraja, Ema Rahmawati, Cut Nur Cinthia Alamanda, Ryan Bayusantika Ristandi, Rifky Walujayati Rachman, Rini Robiani, Azzania Fibriani
Summary: This study aimed to characterize asymptomatic and mildly symptomatic patients with COVID-19 by observing changes in gene expression profiles and possible bacterial coinfection. The results showed differential gene regulation between the asymptomatic and mildly symptomatic groups, with modulation of RNA transport observed in both groups and bacterial coinfection in symptomatic patients. Additionally, no link was found between viral variants and disease severity.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Genetics & Heredity
Dhawale Ramesh Narayanrao, R. S. Tomar, Padhiyar Sm, Kheni Jasminkumar, Gulwe Ashish, Nitin Mahendra Chauhan, Suresh Chandra Singh, Vijay Upadhye, Mohammed Kuddus, Laxmikant Kamble, Sunil Tulshiram Hajare
Summary: This study aimed to identify potential genes and metabolites associated with drought resistance in the little millet Panicum sumatrense. The results revealed the differential expression of key genes in the drought-resistant genotype and highlighted the role of polyamines in drought stress tolerance.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Plant Sciences
Sureshkumar N. Mesara, Kirtan P. Dave, Ramalingam B. Subramanian
Summary: This study elucidates the positive physiological effects of pyraclostrobin on tomato plants through transcriptome analysis, identifying metabolic pathways and key genes responsible for these effects.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2022)
Article
Horticulture
Muhammad Aamir Manzoor, Guohui Li, Wang Xinya, Mengna Wang, Yu Zhao, Irfan Ali Sabir, Iftikhar Hussain Shah, Han Wang, Muhammad Abdullah, Gyung-Tae Kim, Cheng Song, Yongping Cai
Summary: This study systematically characterized and named 15 PbAL genes in Pyrus bretschenedri, and found that they could be classified into six subfamilies. Expression patterns of PbAL genes varied in different organs and fruit development stages, and stress-related cis-acting elements were found in their promoter regions. Furthermore, PbAL genes showed differential expression under IAA, GA, ME, and ABA treatments.
SCIENTIA HORTICULTURAE
(2023)
Article
Biochemistry & Molecular Biology
Himani Punia, Jayanti Tokas, Anurag Malik, Sonali Sangwan, Anju Rani, Shikha Yashveer, Saleh Alansi, Maha J. Hashim, Mohamed A. El-Sheikh
Summary: Salinity stress is a major concern for global food security, and understanding the mechanisms that enable plants to survive under salt stress is crucial. Sorghum has become a focus of research in this area, with a systematic analysis of differentially expressed genes and their functions revealing involvement in important metabolic processes under high salinity. The findings highlight the potential for developing salt-resistant sorghum lines by targeting genes involved in response to salinity.
Review
Neurosciences
Tyler S. Nelson, Bradley K. Taylor
Summary: In summary, the accelerating basic science literature reveals that spinal neuropeptide Y inhibits chronic pain by targeting the Y1 receptor, primarily located in key sites of pain transmission in the dorsal horn. Selective ablation of spinal Y1-INs attenuates hypersensitivity, while pharmacological activation of Y1 inhibits both pain and itch, suggesting Y1-INs as a promising therapeutic target for chronic pain and itch.
PROGRESS IN NEUROBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Di Xie, Bernardo Stutz, Feng Li, Fan Chen, Haining Lv, Matija Sestan-Pesa, Jonatas Catarino, Jianlei Gu, Hongyu Zhao, Christopher E. Stoddard, Gordon G. Carmichael, Marya Shanabrough, Hugh S. Taylor, Zhong-Wu Liu, Xiao-Bing Gao, Tamas L. Horvath, Yingqun Huang
Summary: The research reveals that TET3 plays a critical central role in regulating appetite and energy metabolism in adult mouse AGRP neurons, and also has an unexpected dual role in controlling other complex behaviors through AGRP neurons.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biotechnology & Applied Microbiology
Eslam M. Abdel-Salam, Mohammad Faisal, Abdulrahman A. Alatar, Ahmed A. Qahtan, Perwez Alam
Summary: The study on fringed rue (Ruta chalepensis L.) focused on changes in transcriptomic landscape across leaf, stem, and root tissues to identify genes responsible for rutin biosynthesis. Using comparative transcriptome sequencing, it was found that leaf tissues had the highest rutin content, with up-regulated genes related to rutin biosynthesis compared to roots. This dataset will serve as a valuable public resource for future genomics and transcriptomic studies in R. chalepensis.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Agronomy
Jiwei Zhu, Anirudh Dhammi, Jaap B. van Kretschmar, Edward L. Vargo, Charles S. Apperson, R. Michael Roe
PEST MANAGEMENT SCIENCE
(2018)
Article
Parasitology
Francisco Posada-Florez, Daniel E. Sonenshine, Noble Egekwu, Clifford Rice, Robert Lupitskyy, Steven C. Cook
Article
Multidisciplinary Sciences
Francisco Posada-Florez, Anna K. Childers, Matthew C. Heerman, Noble I. Egekwu, Steven C. Cook, Yanping Chen, Jay D. Evans, Eugene V. Ryabov
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Roger D. Lawrie, Robert D. Mitchell, Anirudh Dhammi, Andrew Wallace, Ernest Hodgson, R. Michael Roe
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2020)
Article
Entomology
Jean M. Deguenon, Claudia Riegel, Erin R. Cloherty-Duvernay, Kaiying Chen, David A. Stewart, Bo Wang, David Gittins, Larissa Tihomirov, Charles S. Apperson, Marian G. McCord, R. Michael Roe
Summary: Malaria, dengue, yellow fever, and the Zika and West Nile Viruses are major vector-borne diseases transmitted by mosquitoes, with over 80% of the world's population at risk. A novel mechanical insecticide derived from volcanic rock shows potential as a residual spray against mosquitoes.
JOURNAL OF MEDICAL ENTOMOLOGY
(2021)
Article
Entomology
Kun Luan, Andre J. West, Marian G. McCord, Emiel A. DenHartog, Quan Shi, Isa Bettermann, Jiayin Li, Nicholas Travanty, Robert D. Mitchell, Grayson L. Cave, John B. Strider, Yongxin Wang, Florian Neumann, Tobias Beck, Charles S. Apperson, R. Michael Roe
Summary: Researchers have developed non-insecticidal cloth and garments that effectively prevent mosquito bites, with good comfort and protection; they have developed prototype textiles and garments that resist biting from Aedes aegypti mosquitoes based on a mathematical model and experimental validation, showing higher bite resistance compared to insecticide-treated clothing.
Article
Agronomy
Grayson L. Cave, Andre J. West, Marian G. McCord, Bryan Koene, J. Benjamin Beck, Jean M. Deguenon, Kun Luan, R. Michael Roe
Summary: Pesticide-free, 3-D, spacer fabrics were investigated as a potential insect barrier for organic farming. The fabrics showed excellent resistance to penetration by thrips and caterpillars, and covered cabbage plants exhibited significantly faster growth.
Article
Microbiology
Loganathan Ponnusamy, Reuben Garshong, Bryan S. McLean, Gideon Wasserberg, Lance A. Durden, Dac Crossley, Charles S. Apperson, R. Michael Roe
Summary: A study in North Carolina, USA found evidence of Rickettsia infection in chiggers, suggesting a potential public health risk that warrants further investigation.
Article
Agronomy
John Efromson, Roger Lawrie, Thomas Jedidiah Jenks Doman, Matthew Bertone, Aurelien Begue, Mark Harfouche, Dominic Reisig, R. Michael Roe
Summary: Rapid and accurate insect identification is crucial for pest management and agriculture. This study developed a machine learning approach using a convolutional neural network to identify the eggs of two caterpillar species with over 99% accuracy. By utilizing a multi-camera array microscope and automated image-processing pipeline, a dataset of approximately 5500 images was rapidly constructed for training and testing the network.
Article
Entomology
Kun Luan, Marian G. McCord, Andre J. West, Grayson Cave, Nicholas V. Travanty, Charles S. Apperson, R. Michael Roe
Summary: Researchers have developed a low-voltage mosquito-resistant cloth that can prevent blood feeding by mosquitoes, while being flexible and breathable. The design is based on mosquito morphometrics, a novel 3-D textile, and a DC resistor-capacitor. The results demonstrated the feasibility of using biomimetic technology to repel mosquitoes and prevent blood feeding using minimal energy consumption.
Article
Biochemistry & Molecular Biology
Kaiying Chen, Jean Marcel Deguenon, Roger D. Lawrie, R. Michael Roe
Summary: In this study, the efficacy of diatomaceous earth (DE; Celite) and a volcanic glass bio-mimic (Imergard) against sand flies was examined. The results showed that exposure to Imergard and Celite killed 50% of adult sand flies in 13.08 and 7.57 hours, respectively. The biominerals showed promise as alternative methods for controlling sand flies and leishmaniasis disease.
Article
Microbiology
Grayson L. Cave, Elise A. Richardson, Kaiying Chen, David W. Watson, R. Michael Roe
Summary: Ticks in the USA are important vectors of disease-causing microbes. Chemical acaricides and repellents are commonly used for tick control, but resistance is a concern. Imergard, a volcanic glass, showed potential as a tick control method.
Article
Immunology
Kaiying Chen, Nicholas V. Travanty, Reuben Garshong, Dac Crossley, Gideon Wasserberg, Charles S. Apperson, R. Michael Roe, Loganathan Ponnusamy
Summary: This study reports the molecular detection of Orientia species in free-living Eutrombicula chiggers collected in an area in North Carolina, USA, where spotted fever group rickettsiae infections are endemic. Scrub typhus, caused by Orientia spp., is a rickettsial disease transmitted by infected larval trombiculid mites (chiggers).
EMERGING INFECTIOUS DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Anirudh Dhammi, Jaap B. van Krestchmar, Jiwei Zhu, Loganathan Ponnusamy, Fred Gould, Dominic Reisig, Ryan W. Kurtz, R. Michael Roe
Summary: Increased feeding rate is a behavioral mechanism for reducing caterpillar susceptibility to Bt toxins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Environmental Sciences
Kaiying Chen, R. Michael Roe, Loganathan Ponnusamy
Summary: Chiggers are the larval stage of mites that are important medically and in veterinary contexts. Some species can transmit scrub typhus disease. More research is needed on chiggers in the US, including identification, life history, and their role in disease transmission.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)