Article
Biochemistry & Molecular Biology
Sanjana Mahadev Bhat, Jane Q. Yap, Oscar A. Ramirez-Ramirez, Philippe Delmotte, Gary C. Sieck
Summary: In this study, a quantitative histochemical technique was used to measure the activity of succinate dehydrogenase (SDH) in the inner mitochondrial membrane of human airway smooth muscle (hASM) cells. The technique involved measuring the change in optical density (OD) caused by the reduction of a reaction indicator (NBT) using a confocal microscope. This method was found to be rigorous, reproducible, and reliable for measuring mitochondrial function in individual cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Ke Cao, Jie Xu, Wenli Cao, Xueqiang Wang, Weiqiang Lv, Mengqi Zeng, Xuan Zou, Jiankang Liu, Zhihui Feng
Summary: Mitochondrial succinate dehydrogenase (SDH) is an enzyme complex that plays a crucial role in regulating mitochondrial metabolism and human health. Recent studies have revealed the dynamic involvement of SDH assembly process in various diseases. Perturbation of SDH assembly in different tissues can lead to distinct pathophysiological changes, highlighting the importance of understanding the intricate SDH assembly process in human health and diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Sui Lay, Xavier Pearce, Oana Sanislav, Paul Robert Fisher, Sarah Jane Annesley
Summary: Mitochondrial Complex II is composed of four core subunits and mutations in any of these subunits can result in lowered Complex II activity. However, mutations in different subunits are associated with distinct patterns of clinical disease. In this study, knockdown of individual Complex II subunits in Dictyostelium discoideum revealed specific phenotypic defects and mitochondrial dysfunction associated with SdhA knockdown. These defects were mediated by the chronic activation of AMP-activated protein kinase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Pavla Stankova, Otto Kucera, Eva Peterova, Moustafa Elkalaf, David Rychtrmoc, Jan Melek, Miroslav Podhola, Veronika Zubanova, Zuzana Cervinkova
Summary: The study found that after consuming a Western-style diet for 30 weeks, mice showed reduced succinate-activated respiration, decreased SDH activity, reduced expression of the SDH activator sirtuin 3 and SDH subunits, and increased succinate levels. The gene and protein expression of SUCNR1 also decreased in the livers of mice fed the diet, but no signs of oxidative damage were observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Hiran A. Prag, Michael P. Murphy, Thomas Krieg
Summary: In the context of myocardial infarction, the burst of superoxide generated by reverse electron transport (RET) at complex I in mitochondria is a crucial trigger for damage during ischaemia/reperfusion (I/R) injury. Here we outline the necessary conditions for superoxide production by RET at complex I and how it can occur during reperfusion. In addition, we explore various pathways that are implicated in generating the conditions for RET to occur and suggest potential therapeutic strategies to target RET, aiming to achieve cardioprotection.
BASIC RESEARCH IN CARDIOLOGY
(2023)
Article
Medicine, Research & Experimental
Ana Cristina S. Bombaca, Leonardo A. Silva, Otavio Augusto Chaves, Lorrainy S. da Silva, Juliana M. C. Barbosa, Ari M. da Silva, Aurelio B. B. Ferreira, Rubem F. S. Menna-Barreto
Summary: This study introduces a novel anti-protozoan drug N4 and its mechanism of action against Trypanosoma cruzi, showing high activity in all parasite stages and indicating it as a potential alternative treatment for Chagas disease. The results presented here may serve as a good starting point for the development of alternative treatments for Chagas disease and for understanding the mechanism of naphthoimidazoles in T. cruzi.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemical Research Methods
Matthew J. Fogarty, Sabhya Rana, Carlos B. Mantilla, Gary C. Sieck
Summary: The new method of using fluorescent imaging for assessing mitochondrial volume density in PhMNs demonstrated comparable results to the traditional EM method, although EM was able to distinguish finer mitochondrial morphology compared to MitoTracker fluorescence. The major advantages of using fluorescent imaging over EM methods include the assessment of a larger sample size and unambiguous identification of motor neurons belonging to a specific motor neuron pool.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Article
Physiology
Alyssa D. Brown, Matthew J. Fogarty, Leah A. Davis, Debanjali Dasgupta, Carlos B. Mantilla, Gary C. Sieck
Summary: Two weeks of diaphragm inactivity caused by C2SH resulted in reduced mitochondrial volume density, mitochondrial fragmentation, and a reduction of SDHmax in type I and IIa DIAm fibers. Type I and IIa DIAm fibers were more sensitive to inactivation compared to type IIx/IIb fibers. These findings suggest that mitochondria in DIAm fibers are adaptable to varying levels of activity.
JOURNAL OF APPLIED PHYSIOLOGY
(2022)
Article
Cell & Tissue Engineering
Kaito Harada, Takashi Yahata, Makoto Onizuka, Takamasa Ishii, Abd Aziz Ibrahim, Eri Kikkawa, Yoichi Gondo, Kiyoshi Ando
Summary: This study reveals that mitochondrial complex II (MCII) plays an essential role in long-term hematopoiesis and hematopoietic reconstitution. Dysfunctions in MCII result in increased reactive oxygen species (ROS) accumulation and DNA damage in hematopoietic stem cells (HSCs), leading to premature senescence.
Review
Medicine, Research & Experimental
Eman M. Ragab, Doaa M. El Gamal, Tarek M. Mohamed, Abeer A. Khamis
Summary: Pancreatic adenocarcinoma (PDAC) and lung cancer are expected to be the most common types of cancer in the future. Chyrsin, a natural flavonoid, has biological anti-cancer effects. Chitosan-chrysin nano-formulations are widely used in medicine due to their improved bioavailability and efficacy.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2022)
Editorial Material
Cell Biology
Natasha Lopes Fischer, Sunny Shin, Igor E. Brodsky
Summary: Itaconate and iNOS work together to regulate NLRP3 inflammasome activity, reducing cytokine secretion and cell death.
Article
Biochemistry & Molecular Biology
Hoi-Shan Wong, Martin D. Brand, Vojtech Mezera, Pratiksha Dighe, Simon Melov, Akos A. Gerencser, Ramzi F. Sweis, Marina Pliushchev, Zhi Wang, Tim Esbenshade, Bryan McKibben, Stephan Riedmaier, Martin D. Brand
Summary: The study demonstrated the effectiveness of novel variants of S1QELs in vivo, showing that mitochondrial site I-Q plays a significant role in driving pathology in Sod2(-/-) mice, while site IIIQo does not significantly contribute to mitochondrial superoxide production in these mice. Treatment with S1QEL352 and S1QEL712 improved cardiac and hepatic pathologies in Sod2(-/-) mice, confirming the importance of superoxide production from mitochondrial site I-Q in hepatic steatosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Adam J. Rauckhorst, Liping Yu, Brian D. Fink, Ritu Som, Eric B. Taylor, William I. Sivitz
Summary: Under low inner mitochondrial membrane potential, oxaloacetate accumulates in organelles and inhibits succinate dehydrogenase, leading to decreased complex II-energized respiration. This process is more active in muscle than in interscapular brown adipose tissue (IBAT) mitochondria. In IBAT mitochondria, oxaloacetate is decarboxylated to pyruvate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Biochemistry & Molecular Biology
Margo Dona, Kim Neijman, Henri J. L. M. Timmers
Summary: Phaeochromocytomas and paragangliomas are rare neuroendocrine tumors, with mutations in succinate dehydrogenase subunit B being the most common indicator for malignancy. There is currently no curative therapy available for patients with metastases, and lack of suitable model systems has hindered therapy development.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2021)
Article
Physiology
Thamires Siqueira Oliveira, Anderson Teixeira Santos, Cherley Borba Vieira Andrade, Johnatas Dutra Silva, Natalia Blanco, Nazareth de Novaes Roche, Juliana Woyames, Pedro Leme Silva, Patricia Rieken Macedo Rocco, Wagner Seixas da-Silva, Tania Maria Ortiga-Carvalho, Flavia Fonseca Bloise
Summary: This study found that sepsis can lead to morphological changes in the diaphragm, increased mitochondrial damage, and impaired function, suggesting that diaphragm damage during sepsis is associated with mitochondrial dysfunction.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Physiology
Matthew J. Fogarty, Elizabeth Ann L. Enninga, Eniola R. Ibirogba, Rodrigo Ruano, Gary C. Sieck
Summary: Congenital diaphragmatic hernia (CDH) is characterized by incomplete partitioning of the thoracic and abdominal cavities by the diaphragm muscle. In a rodent model of Nitrofen-induced CDH, it was found that CDH pups had reduced diaphragm muscle strength, fiber density, and size compared to nonlesioned littermates and Control pups. These findings suggest that diaphragm muscle weakness contributes to the morbidity of CDH.
JOURNAL OF APPLIED PHYSIOLOGY
(2021)
Article
Biochemical Research Methods
Matthew J. Fogarty, Sabhya Rana, Carlos B. Mantilla, Gary C. Sieck
Summary: The new method of using fluorescent imaging for assessing mitochondrial volume density in PhMNs demonstrated comparable results to the traditional EM method, although EM was able to distinguish finer mitochondrial morphology compared to MitoTracker fluorescence. The major advantages of using fluorescent imaging over EM methods include the assessment of a larger sample size and unambiguous identification of motor neurons belonging to a specific motor neuron pool.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Letter
Neurosciences
Matthew J. Fogarty
Article
Medicine, Research & Experimental
Antoine Zelicourt, Abdallah Fayssoil, Mbarka Dakouane-Giudicelli, Isley De Jesus, Ahmed Karoui, Faouzi Zarrouki, Florence Lefebvre, Arnaud Mansart, Jean-Marie Launay, Jerome Piquereau, Mariana G. Tarrago, Marcel Bonay, Anne Forand, Sophie Moog, France Pietri-Rouxel, Elise Brisebard, Claudia C. S. Chini, Sonu Kashyap, Matthew J. Fogarty, Gary C. Sieck, Mathias Mericskay, Eduardo N. Chini, Ana Maria Gomez, Jose-Manuel Cancela, Sabine Porte
Summary: This study demonstrates that CD38 plays an active role in the pathophysiology of Duchenne muscular dystrophy (DMD). Deletion of CD38 in mdx mice leads to restored heart function and structure, improved skeletal muscle performance, reduced inflammation and senescence markers, and restored muscle NAD(+) levels. Treatment with CD38 inhibitors also improves skeletal muscle performance in DMD mice. These findings suggest that selective anti-CD38 therapeutic intervention could be a promising approach for treating DMD patients.
EMBO MOLECULAR MEDICINE
(2022)
Article
Physiology
Matthew J. Fogarty, Joline E. Brandenburg, Wen-Zhi Zhan, Gary C. Sieck
Summary: This study quantified transdiaphragmatic pressure deficits, diaphragm muscle weakness, and fiber type-specific atrophy in spa mutant mice, providing insights into the respiratory dysfunctions in spastic cerebral palsy (sCP).
JOURNAL OF APPLIED PHYSIOLOGY
(2022)
Article
Physiology
Alyssa D. Brown, Matthew J. Fogarty, Leah A. Davis, Debanjali Dasgupta, Carlos B. Mantilla, Gary C. Sieck
Summary: Two weeks of diaphragm inactivity caused by C2SH resulted in reduced mitochondrial volume density, mitochondrial fragmentation, and a reduction of SDHmax in type I and IIa DIAm fibers. Type I and IIa DIAm fibers were more sensitive to inactivation compared to type IIx/IIb fibers. These findings suggest that mitochondria in DIAm fibers are adaptable to varying levels of activity.
JOURNAL OF APPLIED PHYSIOLOGY
(2022)
Article
Neurosciences
Matthew J. J. Fogarty, Obaid U. U. Khurram, Carlos B. B. Mantilla, Gary C. C. Sieck
Summary: The study suggests that chronic TrkB kinase inhibition in TrkB(F616) rats results in a significant worsening of diaphragm neuromuscular transmission, which acute BDNF treatment cannot rescue. Additionally, chronic TrkB kinase inhibition does not affect the apposition of pre-synaptic terminals and post-synaptic endplates at diaphragm NMJs.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Physiology
Matthew J. Fogarty, Gary C. Sieck
Summary: Using a rat model, this study found that with aging, there is a loss of larger phrenic motor neurons and motor fibers, as well as demyelination of the larger phrenic alpha motor axons. These changes may contribute to the reduced muscle strength of the diaphragm with age.
PHYSIOLOGICAL REPORTS
(2023)
Article
Physiology
Matthew J. Fogarty, Sabhya Rana, Carlos B. Mantilla, Gary C. Sieck
Summary: Type S and FR motor units, consisting of smaller phrenic motor neurons, are regularly recruited to meet indefatigable ventilatory requirements. In contrast, Type FF motor units, consisting of larger phrenic motor neurons, are infrequently recruited for expulsive straining and airway defense maneuvers. This difference in activation history is reflected in the mitochondrial volume density (MVD), with smaller PhMNs having higher MVD than larger PhMNs. In proximal dendrites, this trend is reversed, with larger PhMNs having higher MVD than smaller PhMNs, likely due to the maintenance requirements for the larger dendritic arbor of FF PhMNs.
JOURNAL OF APPLIED PHYSIOLOGY
(2023)
Article
Neurosciences
Matthew J. J. Fogarty, Wen-Zhi Zhan, Vincent F. F. Simmon, Peter W. W. Vanderklish, Stella T. T. Sarraf, Gary C. C. Sieck
Summary: This study investigates the effects of C2SH on diaphragm muscle and transdiaphragmatic pressure, as well as the potential therapeutic effect of SPG302. The results show that immediate deficits in diaphragm EMG activity and transdiaphragmatic pressure occur after C2SH surgery, while treatment with SPG302 can enhance the recovery of diaphragm EMG and transdiaphragmatic pressure within 14 days post-surgery.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Neurosciences
Matthew J. Fogarty, Debanjali Dasgupta, Obaid U. Khurram, Gary C. Sieck
Summary: Inhibition of TrkB signaling alone in adult TrkBF616 rats leads to loss of phrenic motor neurons, morphological degeneration, and deficits in retrograde axonal uptake/transport.
MOLECULAR AND CELLULAR NEUROSCIENCE
(2023)
Article
Physiology
Leah A. Davis, Matthew J. Fogarty, Alyssa Brown, Gary C. Sieck
Summary: The mammalian neuromuscular junction (NMJ) is responsible for transmitting signals from the nervous system to muscle fibers. Motor units, which vary in activation frequency, force generation, and susceptibility to fatigue, display distinct structural and functional phenotypes in their NMJs. NMJs are highly plastic and undergo changes throughout the lifespan and under pathological conditions.
COMPREHENSIVE PHYSIOLOGY
(2022)
Article
Physiology
Alyssa R. Mickle, Jesus D. Penaloza-Aponte, Richard Coffey, Natale A. Hall, David Baekey, Erica A. Dale
Summary: Closed-loop epidural stimulation (CL-ES) can improve respiratory deficits caused by cervical spinal cord injury, restoring diaphragm activity and enhancing contralateral activity. This treatment has the potential to lead to lasting recovery and device independence.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Shawn Miller Jr, Edgar Juarez Lopez, Jessica M. L. Grittner, Brendan J. Dougherty
Summary: This study tested the impact of CO2 supplementation at different concentrations on ventilatory long-term facilitation (vLTF) in rats and found that 2% CO2 supplementation during and after acute, intermittent hypoxia (AIH) was sufficient to maintain isocapnia and induce significant vLTF.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Behnam Vafadari, Yoshitaka Oku, Charlotte Tacke, Ali Harb, Swen Huelsmann
Summary: The preBo·tzinger Complex (preBo·tC) in the brainstem plays a critical role in generating respiratory rhythm. This study aimed to investigate the activity of inhibitory glycinergic neurons in the preBo·tC of anesthetized mice. Using juxtacellular recordings and optogenetic activation, the researchers were able to identify and characterize the activity pattern of these inhibitory neurons in relation to the breathing rhythm.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Stephen M. Johnson, Maia G. Gumnit, Sarah M. Johnson, Tracy L. Baker, Jyoti J. Watters
Summary: Low-level activation of mu-opioid receptors can increase the amplitude of inspiratory bursts in neonatal rat brainstem-spinal cord preparations. Endomorphin-2, an endogenous ligand for these receptors, was found to have similar effects. Disinhibition of inhibitory synaptic transmission may not be involved in the changes induced by endomorphin-2, and different mechanisms may underlie the increase in burst amplitude and decrease in burst frequency.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Raphael Martins de Abreu, Beatrice Cairo, Patricia Rehder-Santos, Claudio Donisete da Silva, Etore De Favari Signini, Juliana Cristina Milan-Mattos, Camila Akemi Sakaguchi, Aparecida Maria Catai, Alberto Porta
Summary: The purpose of this study was to determine the association between exercise capacity based on peak oxygen uptake (VO2peak) and resting cardiorespiratory coupling (CRC) levels. The results showed that resting CRC values were associated with exercise capacity in athletes but not in non-athletes.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Sarah M. Russel, Raluca E. Gosman, Katherine Gonzalez, Joshua Wright, Dennis O. Frank-Ito
Summary: This study aimed to characterize the response of different nasal morphologies to airflow-related variables during rapid/deep inspiration. The results showed that notched nasal vestibules had higher resistance values and airflow velocities, while standard nasal vestibules had higher mucosal heat flux. Different nasal phenotypes may predispose individuals to exercise-induced rhinitis.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Ivan Poliacek, Lukas Martvon, Michal Simera, Marcel Veternik, Jakub Misek, Lucia Cibulkova, Kimberly E. Iceman, Donald C. Bolser, Teresa Pitts
Summary: An animal model study evaluated the effects of an abdominal incision on cough and swallow, revealing that abdominal wall manipulations can increase pharyngeal muscle activity during swallow without affecting the cough reflex. Swallowing tended to occur more during the inspiratory phase. The results highlight the important role of abdominal wall sensory feedback in regulating swallow motor patterns.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Rui Yu, Tatsuma Okazaki, Yuzhuo Ren, Junko Okuyama, Satoru Ebihara, Shin-Ichi Izumi
Summary: Body postures significantly influence respiratory muscle force, cough pressure, subjective ease of coughing, and pulmonary function. The 60-degree semi-recumbent posture and sitting posture show better results compared to the supine posture.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Suzuna Sato, Koji Ishida, Noriko I. Tanaka, Keisho Katayama
Summary: Respiratory muscle endurance training has beneficial effects on whole-body endurance performance. A novel high-intensity interval (HII) protocol is found to enhance the efficacy of respiratory muscle training programs.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Letter
Physiology
R. Arieli
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Correction
Physiology
Ivan Poliacek, Michal Simera, Marcel Veternik, Zuzana Kotmanova, Teresa Pitts, Jan Hanacek, Jana Plevkova, Peter Machac, Nadezda Visnovcova, Jakub Misek, Jan Jakus
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Dimitrios I. Bourdas, Nickos D. Geladas
Summary: This study examined the effects of breath-hold training on the performance of novice and elite breath-hold divers. The results showed that breath-hold training significantly improved red blood cell concentration, hemoglobin oxygen saturation steady state duration, and breath-hold time in novice divers. Elite divers had better breath-hold performance and higher peak mean arterial pressure compared to novices. The study suggests that breath-hold training can enhance the performance of divers.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)