Article
Environmental Sciences
Kang-Na Wei, Xin-Jie Wang, Zhao-Cheng Zeng, Ruo-Ting Gu, Shu-Zi Deng, Jiang Jiang, Chang-Long Xu, Wei Li, Hai-Long Wang
Summary: The study revealed the toxic effects of PFOS on mouse oocyte maturation, including reduced polar body extrusion rate and symmetrical cell division. PFOS caused abnormal cytoskeleton in oocytes, inhibited meiotic progression, and disrupted oocyte quality and histone modifications.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Cell Biology
Xue Yue, Sai-Li Liu, Jia-Ni Guo, Tie-Gang Meng, Xin-Ran Zhang, Hong-Xia Li, Chun-Ying Song, Zhen-Bo Wang, Heide Schatten, Qing-Yuan Sun, Xing-Ping Guo
Summary: Epitalon can delay the in vitro aging process of oocytes by reducing intracellular reactive oxygen species, decreasing spindle defects, and increasing mitochondrial activity.
Article
Medicine, Research & Experimental
Ruiqi Li, Enhong Li, Gumina Kamili, Songbang Ou, Dongzi Yang
Summary: This study evaluated the effect of resveratrol (RSV) on embryonic development in mice during the process of superovulation. The results showed that RSV treatment significantly improved blastocyst rate in parthenogenetically activated oocytes and naturally fertilized oocytes groups, as well as in aged mice. Additionally, RSV treatment increased mitochondrial function and upregulated mitochondrial biogenesis related genes in cumulus cells, indicating an improvement in oocyte quality for both young and aged mice.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Cell Biology
Hao-Lin Zhang, Yi Xu, Jia-Qian Ju, Zhen-Nan Pan, Jing-Cai Liu, Shao-Chen Sun
Summary: This study compared the global RNA transcription pattern of oocytes from in vitro and in vivo maturation, identifying 1,864 differentially expressed genes. The changes were mainly related to environmental adaption, metabolism, and genetic expression processes, suggesting that in vitro maturation of mouse oocytes resulted in metabolism and genetic expression changes due to environmental differences compared with in vivo matured oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Di Wu
Summary: The germinal vesicle (GV) stage is a critical transition point in mammalian oocyte development that determines oocyte quality through active RNA degradation and transcriptome reprofiling. Oocyte RNA-seq has revealed differences between non-surrounded nucleolus (NSN) and surrounded nucleolus (SN) phases. Single oocyte RNA-seq (soRNA-seq) shows higher reproducibility and a better illustration of gene changes during oocyte development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhi-Ming Ding, Yang-Wu Chen, Yong-Sheng Wang, Muhammad Jamil Ahmad, Sheng-Ji Yang, Ze-Qun Duan, Ming Liu, Cai-Xia Yang, Jia-Jun Xiong, Ai-Xin Liang, Li-Jun Huo
Summary: Studies have shown that exposure to gossypol can have negative effects on the maturation of mouse oocytes, leading to disrupted spindle structure and chromosome dynamics, impaired mitochondrial function, oxidative stress, and early apoptosis.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Genetics & Heredity
Wataru Yamazaki, Dunarel Badescu, Seang Lin Tan, Jiannis Ragoussis, Teruko Taketo
Summary: The sex chromosome complement plays a role in regulating gene transcription in oocytes, with XY oocytes showing abnormal chromatin configuration, mitochondria distribution, and de novo transcription compared to XX or XO oocytes near the end of the growth phase.
FRONTIERS IN GENETICS
(2021)
Article
Cell Biology
Ping-Shuang Lu, Lan-Ping Xie, Xiao-Han Kong, Yi Xu, Shao-Chen Sun
Summary: In this study, exposure to POD significantly reduced the developmental competence of mouse oocytes, affecting the distribution and function of organelles such as endoplasmic reticulum and Golgi apparatus. This exposure induced vesicle-based protein transport disorder and aberrant accumulation of lysosomes, as well as disruption of the perinuclear distribution of mitochondria.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yue Xue, Tie-Gang Meng, Ying-Chun Ouyang, Sai-Li Liu, Jia-Ni Guo, Zhen-Bo Wang, Heide Schatten, Chun-Ying Song, Xing-Ping Guo, Qing-Yuan Sun
Summary: This study found that Miro1 plays a crucial role in the maturation process of mouse oocytes by regulating mitochondrial homeostasis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Reproductive Biology
Takuya Sato, Mao Hamazaki, Yuki Inoue, Sogo Aoki, Yuichi Koshiishi, Koumei Shirasuna, Hisataka Iwata
Summary: This study investigated the effects of low ethanol exposure on bovine oocytes. The results showed that even concentrations as low as 0.1% ethanol during in vitro maturation significantly affected oocyte metabolism and histone configuration of embryos. Ethanol exposure increased mitochondrial DNA copy number and protein levels, blastulation rate, ATP content and membrane potential, while decreasing lipid content and glucose consumption.
Article
Cell Biology
Chao-Ying Zhao, Lin-Lin Hu, Chun-Hua Xing, Xiang Lu, Shao-Chen Sun, Yu-Xia Wei, Yan-Ping Ren
Summary: Acrylamide exposure disrupts the distribution and functions of organelles in mouse oocytes, leading to reduced developmental competence.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Reproductive Biology
Qiaochu Wang, Karla J. Hutt
Summary: Cisplatin can decrease mitochondrial membrane potential in oocytes, leading to cell death. Five weeks after treatment, the number of mature oocytes harvested from cisplatin treated mice was significantly lower, but the surviving oocytes had normal mitochondrial parameters.
JOURNAL OF OVARIAN RESEARCH
(2021)
Review
Reproductive Biology
Qiuzi Shen, Yu Liu, Honggang Li, Ling Zhang
Summary: Mitophagy is crucial for maintaining mitochondria homeostasis and cell survival. While aberrant mitochondria can be removed through mitophagy during early stages of oogenesis, dysfunctional mitochondria can be transmitted from oocytes to embryos when mitophagy is not actively initiated. Granulosa cells can improve mitochondrial function through mitophagy, enhancing oocyte developmental capacity. Research on substances and environmental conditions influencing mitophagy in oocytes and granulosa cells may provide new strategies for improving oocyte quality in assisted reproductive technology treatment.
BIOLOGY OF REPRODUCTION
(2021)
Article
Endocrinology & Metabolism
Shi-meng Guo, Xing-ping Liu, Qing Tian, Cai-feng Fei, Yi-ran Zhang, Zhi-ming Li, Ying Yin, Ximiao He, Li-quan Zhou
Summary: This study reveals the importance of alternative splicing at Ezh2 exon 3 in mouse oocyte development. Ezh2(Short) female mice exhibit decreased fertility and obstructed oogenesis with compromised mitochondrial function in their oocytes.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alejandro de la Fuente, Charles Scoggin, Etta Bradecamp, Soledad Martin-Pelaez, Machteld van Heule, Mats Troedsson, Peter Daels, Stuart Meyers, Pouya Dini
Summary: This study aimed to elucidate the transcriptome dynamics of oocyte maturation by comparing transcriptomic changes during in vitro maturation in both oocytes and their surrounding cumulus cells. The results showed an association between oocyte maturation and pathways relating to nuclear maturation in oocytes and GTPase activity, extracellular matrix organization, and collagen trimers in cumulus cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Yukiko Kawabata, Asuka Kamio, Yuko Jincho, Akihiko Sakashita, Tomoya Takashima, Hisato Kobayashi, Yasuhisa Matsui, Tomohiro Kono
Article
Developmental Biology
Toshihiro Kobayashi, Hisato Kobayashi, Teppei Goto, Tomoya Takashima, Mami Oikawa, Hiroki Ikeda, Reiko Terada, Fumika Yoshida, Makoto Sanbo, Hiromitsu Nakauchi, Kazuki Kurimoto, Masumi Hirabayashi
Article
Cardiac & Cardiovascular Systems
Tomoya Takashima, Sophie Brisset, Asuka Furukawa, Hirohisa Taniguchi, Rika Takeyasu, Akio Kawamura, Yuichi Tamura
Summary: The study demonstrated the feasibility of using MinION nanopore sequencer for genetic analysis of PAH patients, particularly in detecting BMPR2 mutations. The portable nanopore sequencer showed promise as a screening tool for BMPR2 mutations, especially in small laboratories and research groups.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Mami Oikawa, Hisato Kobayashi, Makoto Sanbo, Naoaki Mizuno, Kenyu Iwatsuki, Tomoya Takashima, Keiko Yamauchi, Fumika Yoshida, Takuya Yamamoto, Takashi Shinohara, Hiromitsu Nakauchi, Kazuki Kurimoto, Masumi Hirabayashi, Toshihiro Kobayashi
Summary: The in vitro generation of germ cells from pluripotent stem cells has significant implications for reproductive medicine and animal breeding. In this study, researchers successfully induced functional primordial germ cell-like cells from rat pluripotent stem cells and generated functional spermatids through transplantation. This research provides insights into the mechanisms of in vitro gametogenesis and has implications for broader applications.
Article
Biotechnology & Applied Microbiology
Julien Richard Albert, Toshihiro Kobayashi, Azusa Inoue, Ana Monteagudo-Sanchez, Soichiro Kumamoto, Tomoya Takashima, Asuka Miura, Mami Oikawa, Fumihito Miura, Shuji Takada, Masumi Hirabayashi, Keegan Korthauer, Kazuki Kurimoto, Maxim V. C. Greenberg, Matthew Lorincz, Hisato Kobayashi
Summary: In this study, low input methods were used to analyze gene expression and DNA methylation in embryonic and extraembryonic tissues of rats. The researchers identified 14 and 26 imprinted genes in these tissues, with 10 genes showing imprinted expression in both tissues. Comparative analysis with mice showed conservation of imprinted gene expression and DNA methylation in the embryo proper, but not in extraembryonic tissues. The study also discovered novel non-canonical imprinted genes unique to rats and identified potential mechanisms responsible for species-specific imprinting.
Article
Agriculture, Dairy & Animal Science
Saya Ota, Shinya Ikeda, Tomoya Takashima, Yayoi Obata
Summary: Follicular culture is a useful tool for exploring the developmental competencies and factors related to oocyte growth. Adding high molecular weight polyvinylpyrrolidone and adjusting oxygen concentration can significantly improve blastocyst formation rate.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2021)
Article
Cardiac & Cardiovascular Systems
Hirohisa Taniguchi, Tomoya Takashima, Ly Tu, Raphael Thuillet, Asuka Furukawa, Yoshiko Furukawa, Akio Kawamura, Marc Humbert, Christophe Guignabert, Yuichi Tamura
Summary: Although precapillary pulmonary hypertension is a rare but severe complication in NF1 patients, its association with NF2 remains unknown; a case of a 44-year-old woman diagnosed with idiopathic pulmonary arterial hypertension was found to have a mutation in the NF2 gene; decreased endothelial NF2 protein in the lungs of pulmonary arterial hypertension patients suggests a potential role of NF2 in the development of the condition.
PULMONARY CIRCULATION
(2021)