Cellular and molecular basis of IR3535 perception in Drosophila

BACKGROUND IR3535 is among the most widely used synthetic insect repellents, particularly for the mitigation of mosquito-borne diseases such as malaria, yellow fever, dengue and Zika, as well as to control flies, ticks, fleas, lice and mites. These insects are well-known vectors of deadly diseases that affect humans, livestock and crops. Moreover, global warming could increase the populations of these vectors. RESULTS Here, we performed IR3535 dose-response analyses on Drosophila melanogaster, a well-known insect model organism, using electrophysiology and binary food choice assays. Our findings indicated that bitter-sensing gustatory receptor neurons (GRNs) are indispensable to detect IR3535. Further, potential candidate gustatory receptors were screened, among which GR47a was identified as a key molecular sensor. IR3535 concentrations in the range 0.1-0.4% affected larval development and mortality. In addition, N,N-diethyl-m-toluamide (DEET, another commonly used insecticide) was found to exert synergistic effects when co-administered with IR3535. CONCLUSION Our findings confirmed that IR3535 directly activates bitter-sensing GRNs, which are mediated by GR47a. This relatively safe and highly potent insecticide can be largely used in combination with DEET to increase its efficiency to protect livestock and crops. Collectively, our findings suggest that the molecular sensors elucidated herein could be used as targets for the development of alternative insecticides.

Automated summary

IR3535 is a widely used synthetic insect repellent, particularly for mosquito-borne diseases, and can activate bitter-sensing gustatory receptor neurons mediated by GR47a. When used in combination with DEET, it shows synergistic effects in protecting livestock and crops. This suggests that the molecular sensors identified in this study could be potential targets for alternative insecticide development.