期刊
INFLAMMATORY BOWEL DISEASES
卷 22, 期 12, 页码 2853-2862出版社
OXFORD UNIV PRESS INC
DOI: 10.1097/MIB.0000000000000956
关键词
nutrition in pediatrics; Crohn's disease; intestinal microbiome
资金
- NASPGHAN/CCFA Young Investigator Development award
- Nova Scotia Health Research Foundation (NSHRF) establishment award
- Future Leaders in Inflammatory Bowel Disease (FLIBD) Program grant
- Dalhousie Medical Research Foundation equipment grant
- MacLeod family
- IWK Health Centre Research Associateship grant
- Canadian Institutes of Health Research (CIHR)-CAG-CCC New Investigator Award [20152020: 201412XGP-340307-205026]
- Canadian Foundation of Innovation John R. Evans Leadership fund [35235]
- CIHR-SPOR-Chronic Diseases grant
- NSHRF Development and Innovation Grant
- CIHR [CMF-108026]
- Atlantic Computational Excellence Network [ACEnet 2011 -2285]
Background: Clinical remission achieved by exclusive enteral nutrition (EEN) is associated with marked microbiome changes. In this prospective study of exclusive enteral nutrition, we employ a hierarchical model of microbial community structure to distinguish between pediatric Crohn's disease patients who achieved sustained remission (SR) and those who relapsed early (non-SR), after restarting a normal diet. Methods: Fecal samples were obtained from 10 patients (age 10-16) and from 5 healthy controls (age 9-14). The microbiota was assessed via 16S rRNA sequencing. In addition to standard measures of microbial biodiversity, we employed Bayesian methods to characterize the hierarchical community structure. Community structure between patients who sustained remission (wPCDAI <12.5) up to their 24-week follow-up (SR) was compared with patients that had not sustained remission (non-SR). Results: Microbial diversity was lower in Crohn's disease patients relative to controls and lowest in patients who did not achieve SR. SR patients differed from non-SR patients in terms of the structure and prevalence of their microbial communities. The SR prevalent community contained a number of strains of Akkermansia muciniphila and Bacteroides and was limited in Proteobacteria, whereas the non-SR prevalent community had a large Proteobacteria component. Their communities were so different that a model trained to discriminate SR and non-SR had 80% classification accuracy, already at baseline sampling. Conclusions: Microbial community structure differs between healthy controls, patients who have an enduring response to exclusive enteral nutrition, and those who relapse early on introduction of normal diet. Our novel Bayesian approach to these differences is able to predict sustained remission after exclusive enteral nutrition.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据