期刊
INFECTION GENETICS AND EVOLUTION
卷 37, 期 -, 页码 150-157出版社
ELSEVIER
DOI: 10.1016/j.meegid.2015.11.022
关键词
Shiga toxin (Stx); Stx prototype; Shigella sonnei; S. dysenteriae; Converting phage; Stx-producing Escherichia coli (STEC)
资金
- Hungarian Research Fund (OTKA) [K 81252]
Here we report the first complete nucleotide sequence of a Shiga toxin (Six) converting phage from a Shigella sonnet clinical isolate that harbors stx1 operon, first identified in the chromosome of Shigella dysenteriae type 1. The phage named Shigella phage 75/02 Stx displayed Podoviridae morphology. It proved to be transferable to Escherichia coli K-12 strains, and cytotoxicity of the lysogenized strains was demonstrated in Vero cell cultures. Genomic analysis revealed that the prophage genome is circular and its size is 60,875 nt that corresponds to 76 ORFs. The genome of Shigella phage 75/02 Stx shows a great degree of mosaic structure and its architecture is related to lambdoid phages. All the deduced proteins, including the 37 hypothetical proteins showed significant homologies to Six phage proteins present in databases. The phage uniformly inserted into the ynfG oxidoreductase gene framed by phage integrase and antirepressor genes in parental S. sonnei and in the three lysogenized K-12 strains C600, DH5 alpha. and MG1655. The Stx1 prophage proved to be stable in its bacterial hosts and remained inducible. (C) 2015 Elsevier B.V. All rights reserved.
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