期刊
NANO LETTERS
卷 21, 期 24, 页码 10377-10385出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.1c03683
关键词
in situ coassembly; activatable probe; multimodality; in vivo imaging; alkaline phosphatase
类别
资金
- National Key R&D Program of China [2020YFA0713800]
- National Natural Science Foundation of China [21922406, 21632008, 22076069]
- Fundamental Research Funds for the Central Universities [020514380251]
- Natural Science Foundation of Jiangsu Province [BK20202004, BK20190055, BK20201135]
- Major Scientific Research Project of Jiangsu Commission of Health [ZDA2020007]
- Excellent Research Program of Nanjing University [ZYJH004]
This study presents an enzyme-activatable probe for high sensitivity bimodal imaging of tumors, utilizing an enzyme-induced reaction and coassembly strategy. The method significantly enhances NIR FL and radioactivity imaging sensitivity, facilitating the differentiation of tumor foci from normal tissues in vivo.
Smart near-infrared (NIR) fluorescence (FL) and positron emission tomography (PET) bimodal probes have shown promise for preoperative and intraoperative imaging of tumors. In this paper, we report an enzyme-activatable probe (P-CyFF-Ga-68) and its cold probe (P-CyFF-Ga) using an enzyme-induced fluorogenic reaction and in situ coassembly strategy and demonstrate the utility for NIR FL/PET bimodality imaging of enzymatic activity. P-CyFF-Ga-68 and P-CyFF-Ga can be converted into dephosphorylated CyFF-Ga-68 and CyFF-Ga in response to alkaline phosphatase (ALP) and subsequently coassemble into fluorescent and radioactive nanoparticles (NP-Ga-68). The ALP-triggered in situ formed NP-Ga-68 is prone to anchoring on the ALP-positive HeLa cell membrane, permitting the concurrent enrichment of NIR FL and radioactivity. The enhancements in NIR FL and radioactivity enables high sensitivity and deep-tissue imaging of ALP activity, consequently facilitating the delineation of HeLa tumor foci from the normal tissues in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据