Regulating the T7 RNA polymerase expression in E. coli BL21 (DE3) to provide more host options for recombinant protein production

Protein over-expression in Escherichia coli triggers adaptation analogous to antimicrobial resistance

(2021) Jack James et al.   Microbial Cell Factories

Tunable expression rate control of a growth-decoupled T7 expression system by l-arabinose only

(2021) Patrick Stargardt et al.   Microbial Cell Factories

A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli

(2021) Qi Li et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Bacteriophage Inspired Growth-Decoupled Recombinant Protein Production in Escherichia coli

(2020) Patrick Stargardt et al.   ACS Synthetic Biology

Multicopy Chromosomal Integration Using CRISPR-Associated Transposases

(2020) Yiwen Zhang et al.   ACS Synthetic Biology

Recombinant protein production‐associated metabolic burden reflects anabolic constraints and reveals similarities to a carbon overfeeding response

(2020) Zhaopeng Li et al.   BIOTECHNOLOGY AND BIOENGINEERING

Downregulation of T7 RNA polymerase transcription enhances pET‐based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis

(2020) Xiao‐Man Sun et al.   BIOTECHNOLOGY AND BIOENGINEERING

Synthetic protein-binding DNA sponge as a tool to tune gene expression and mitigate protein toxicity

(2020) Xinyi Wan et al.   Nature Communications

Trade-offs between gene expression, growth and phenotypic diversity in microbial populations

(2019) Juhyun Kim et al.   CURRENT OPINION IN BIOTECHNOLOGY

Tunable Control of an Escherichia coli Expression System for the Overproduction of Membrane Proteins by Titrated Expression of a Mutant lac Repressor

(2017) Seong Keun Kim et al.   ACS Synthetic Biology

Tailoring Escherichia coli for the l-Rhamnose PBAD Promoter-Based Production of Membrane and Secretory Proteins

(2017) Anna Hjelm et al.   ACS Synthetic Biology

Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3)

(2017) Thomas Baumgarten et al.   Scientific Reports

Enhanced protein and biochemical production using CRISPRi-based growth switches

(2016) Songyuan Li et al.   METABOLIC ENGINEERING

Comparative genomics and experimental evolution of Escherichia coli BL21(DE3) strains reveal the landscape of toxicity escape from membrane protein overproduction

(2015) Soon-Kyeong Kwon et al.   Scientific Reports

Interdependence of Cell Growth and Gene Expression: Origins and Consequences

(2010) M. Scott et al.   SCIENCE

Genome Sequences of Escherichia coli B strains REL606 and BL21(DE3)

(2009) Haeyoung Jeong et al.   JOURNAL OF MOLECULAR BIOLOGY

Tuning Escherichia coli for membrane protein overexpression

(2008) S. Wagner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA