Article
Chemistry, Applied
Xin Qi, Duoxia Xu, Jinjin Zhu, Shaojia Wang, Jingwei Peng, Wei Gao, Yanping Cao
Summary: The interaction between lutein dipalmitate and BSA was found to be static with a binding stoichiometry of 1:1. Thermodynamic study and molecular docking confirmed that lutein dipalmitate spontaneously bound to BSA through hydrogen bonds, van der Waals forces, and hydrophobic interactions. The addition of lutein dipalmitate led to a decrease in the alpha-helix content of BSA, as observed through CD analysis.
FOOD HYDROCOLLOIDS
(2021)
Article
Food Science & Technology
Qun Zhang, Wei Fan, Yan Shi, Zongcai Tu, Yueming Hu, Jing Zhang
Summary: This study investigated the interaction between whey protein isolate/soy protein isolate and sucrose esters during spray drying microencapsulation. The results showed that sucrose esters induced conformational changes in the proteins and formed protein-sucrose ester complexes. Increasing the sucrose ester ratio decreased the particle sizes of the complexes. Molecular docking simulations revealed hydrogen bonds, salt bridges, and hydrophobic interactions between the proteins and sucrose esters.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Jia Xu, Yi Huang, Yang Wei, Xinchu Weng, Xinlin Wei
Summary: The interaction mechanism between whey proteins and theaflavin (TF1) in black tea was analyzed. TF1 showed static quenching mechanism with bovine serum albumin (BSA), beta-lactoglobulin (beta-Lg), and alpha-lactoalbumin (alpha-La), as revealed by fluorescence and ultraviolet-visible absorption spectroscopy. TF1 also altered the secondary structure of these proteins, as observed in circular dichroism experiments. Molecular docking simulations indicated that hydrogen bonding and hydrophobic interaction played dominant roles in the interaction between TF1 and BSA/beta-Lg/alpha-La, with binding energies of -10.1 kcal mol(-1), -8.4 kcal mol(-1), and -10.4 kcal mol(-1), respectively.
Article
Spectroscopy
Sujuan Zhu, Xuexue Bai, Jing Zhu, Wen Li, Bing Wang
Summary: The study investigated the interaction between ferulic acid (FA) and pepsin using various spectroscopic techniques and molecular docking. The results showed that FA altered the secondary structure of pepsin and affected its activity through a combination of electrostatic, hydrophobic, and hydrogen bonding forces.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Applied
Ziqi Guo, Yuqi Huang, Jian Huang, Shuyi Li, Zhenzhou Zhu, Qianchun Deng, Shuiyuan Cheng
Summary: The interaction between grape skin anthocyanin extract and gliadin was investigated, and it was found that anthocyanins could effectively form complexes with gliadin and change its conformational properties.
Article
Spectroscopy
Tancong Liao, Yuai Zhang, Xiaojian Huang, Zheng Jiang, Xun Tuo
Summary: Sulfonamides like sulfametoxydiazine (SMD) and sulfamonomethoxine (SMM) were found to bind to human serum albumin (HSA) through hydrogen bonding and van der Waals forces, with SMD showing stronger binding affinity than SMM. The interaction induced conformational changes in HSA, particularly affecting the alpha-helix content, and specific amino acid residues in HSA were identified as key players in the binding process of sulfonamides. Results suggest that hydrogen bonds play a crucial role in the binding affinity between sulfa drugs and HSA, with combined use of SMD and SMM leading to significant variations in binding constants.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Physical
Rong-Juan Zhang, Song-Bo Kou, Lu Hu, Li Li, Jie-Hua Shi, Shao-Liang Jiang
Summary: In this study, the interaction between Baricitinib (BRT) and bovine serum albumin (BSA) was investigated. The results showed a weak binding between BRT and BSA, with van der Waals forces and hydrogen bonds playing a critical role in the complexation process. The findings provide important insights for evaluating the pharmacological characteristics of BRT and designing new drugs.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Spectroscopy
Bingjun Shen, Huiru Yang, Jiaqi Chen, Xiaoyun Liu, Mingyue Zhou
Summary: This study investigated the interaction mechanism between Juglone and DNA through various experimental techniques, revealing that Juglone can quench the fluorescence of DNA, affect the absorption spectrum, enhance resonance scattering peak, and increase the melting point of DNA. The results provide insights into the binding characteristics and thermodynamic properties of Juglone with DNA.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Ankita Jalan, N. Shaemningwar Moyon
Summary: In this study, the interaction between ALP and human serum albumin (HSA) and bovine serum albumin (BSA) was investigated using various spectroscopic techniques. The results showed that ALP significantly quenched the intrinsic fluorescence of both BSA and HSA, with a noticeable red shift in emission maxima. Molecular docking studies revealed moderately strong binding affinity between ALP and BSA/HSA, primarily driven by hydrophobic forces. The spectroscopic studies also indicated that the interaction between ALP and BSA/HSA induced conformational changes.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Fengmao Li, Yufan Fu, Hao Yang, Yunming Tang
Summary: Luteolin effectively inhibits peroxidase activity in a noncompetitive manner, forming a static complex driven by hydrogen bond and hydrophobic interaction. Interaction between luteolin and peroxidase results in intrinsic fluorescence quenching, structural and conformational alterations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Xia Yu, Xinghong Cai, Shuang Li, Liyong Luo, Jie Wang, Min Wang, Liang Zeng
Summary: By utilizing various experimental techniques such as fluorescence spectroscopy and UV-vis absorption spectroscopy, the interaction mechanism between theaflavin-3,3'-digallate (TFDG) and bovine serum albumin (BSA) was studied, demonstrating that hydrophobic interactions and hydrogen bonds play a dominant role in their interaction. This research provides the possibility for further development of nanoparticles with superior biochemical properties and potential exploration of BSA as a transport carrier for TFDG.
Article
Food Science & Technology
Jia-Nan Chen, Hui-Lin Zhao, Yu-Ying Zhang, Da-Yong Zhou, Lei Qin, Xu-Hui Huang
Summary: A recent study examined the interactions between four fermentation-stinky compounds and myofibrillar proteins. The results showed different degrees of binding between the compounds and proteins, with dibutyl phthalate and dimethyl disulfide exhibiting stronger interactions. The addition of hydrophobic bond-disrupting agents was found to improve the flavor of fermented foods.
Article
Spectroscopy
Hailin Wang, Pingfan Rao, Yunjie Qiu, Leiwen Xiang
Summary: In this study, the interaction mechanism between hydroxychloroquine sulfate (HCQ) and collagen was investigated using spectroscopic and computational methods. It was found that HCQ binds to collagen through hydrophobic interactions and hydrogen bonds, leading to changes in the structure of collagen. Molecular docking and dynamic simulations further confirmed the binding mode and structural changes. The findings provide insights into the pharmacological mechanisms of HCQ in the treatment of connective tissue diseases and the application of collagen as a drug carrier.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Md. Abdur Razzak, Shin Sik Choi
Summary: The interaction between ovalbumin (OVA) and isoflavonoid glabridin (GB) was studied through spectroscopic and molecular docking techniques. GB was found to bind to OVA mainly through hydrogen bonding and hydrophobic forces, leading to changes in OVA's secondary structure. Molecular docking simulations supported the experimental findings. Additionally, the GB-OVA complex increased the aqueous solubility of GB.
Article
Spectroscopy
Yangyang Fan, Yanzhou Tao, Guiming Liu, Meizi Wang, Suqing Wang, Li Li
Summary: Engeletin was found to strongly inhibit CYP3A4 and CYP2D6 activities through competitive and mixed inhibition modes, leading to conformational and micro-environmental changes in the two proteins. The strong binding affinity of engeletin to CYPs through hydrogen and van der Waals forces may cause herb-drug interactions.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Food Science & Technology
Shaobo Li, Can Xiang, Yue Ge, Huan Liu, Dequan Zhang, Zhenyu Wang
Summary: The quality characteristics of Taihang and Huanghuai goat meat samples were found to be influenced by pre- and post-rigor states, with pre-rigor meat samples having higher water holding capacity than post-rigor meat samples, and showing differences in aroma and taste profiles.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Huan Liu, Teng Hui, Fei Fang, Shaobo Li, Zhenyu Wang, Dequan Zhang
Summary: The study explored the formation of key aroma compounds in roasted mutton during traditional charcoal roasting. Results showed that samples roasted for different times could be distinguished, with 15 key aroma compounds identified. The roasted mutton exhibited aromas of meaty, fatty, roasty, grassy, and sweet odors after being roasted.
Article
Chemistry, Applied
Huan Liu, Teng Hui, Xiaochun Zheng, Shaobo Li, Xiangru Wei, Pi Li, Dequan Zhang, Zhenyu Wang
Summary: Lipids are important for the flavor quality of roasted mutton, with triglycerides, phosphatidylcholine, and phosphatidylethanolamine being the major lipid classes detected. Certain triglycerides were identified as potential binding lipids for aroma compounds. Differential lipids, especially phosphatidylcholine and phosphatidylethanolamine, were found to contribute significantly to aroma compound formation in roasted mutton. Specific phosphatidylcholine species were highlighted as potential markers for distinguishing roasted mutton.
Article
Food Science & Technology
Shaobo Li, Xin Li, Qianli Ma, Zhenyu Wang, Fei Fang, Dequan Zhang
Summary: This study provides insights into the preferences and purchasing habits of Chinese consumers towards lamb meat. The majority of respondents liked eating lamb meat and preferred using fresh lamb meat for cooking, with stewing being the most popular cooking method. Consumers showed a preference for consuming local lamb meat and expressed concerns about food safety, lamb breed, and meat origin. Additionally, over 65.0% of respondents preferred purchasing traceable lamb meat.
Article
Food Science & Technology
Chunjuan Zhang, Dequan Zhang, Yuanyuan Su, Xiaochun Zheng, Shaobo Li, Li Chen
Summary: This study constructed a convolutional neural network (CNN) image recognition model for the real-time automatic identification of adulterated minced mutton. By comparing and analyzing different CNN models, it was found that ResNet-18, GoogLeNet, and DarkNet-19 models have the best learning effect and can accurately identify different livestock and poultry meat pieces and adulterated minced mutton images. The image learning based on a deep convolutional neural network (DCNN) model provides technical support for the rapid and nondestructive identification of mutton authenticity.
Article
Food Science & Technology
Minghui Gu, Cheng Li, Yuanyuan Su, Li Chen, Shaobo Li, Xin Li, Xiaochun Zheng, Dequan Zhang
Summary: This study monitored the biogenic amine (BA) content in pork during storage and identified a new quality indicator. The results showed that the index system in beef, mutton, and chicken can be used for meat quality evaluation in cold chain logistics.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Liang Li, Cheng Li, Dequan Zhang, Li Chen, Shaobo Li, Wei Wang, Xiaochun Zheng
Summary: This study found a strong correlation between hydrogen sulfide concentration and lamb meat freshness, and successfully developed a multiple parameters fusion model with temperature correction. The proposed model accurately predicts and evaluates the freshness of lamb meat during storage.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Applied
Shaobo Li, Dequan Zhang, Can Xiang, Yue Ge, Huan Liu, Xiaochun Zheng, Li Chen, Zhenyu Wang
Summary: The effect of rigor state on meat batter quality and physicochemical properties of Funiu white goat and Oula sheep meat was studied. Results showed that post-rigor meat batters had lower water holding capacity and immobilized water compared to pre-rigor meat batters. The gel network of Funiu white goat meat batters was denser and smoother than Oula sheep meat batters. Furthermore, pre-rigor meat batters were harder than post-rigor meat batters. Sensitivity to sweet taste varied among the different rigor states. Overall, both pre-rigor Funiu white goat and Oula sheep meat were more suitable for minced meat product production based on gel properties.
Article
Chemistry, Applied
Minghui Gu, Cheng Li, Li Chen, Shaobo Li, Naiyu Xiao, Dequan Zhang, Xiaochun Zheng
Summary: An untargeted metabolomics approach coupled with random forests machine learning algorithm was used to investigate the changes in non-volatile components and metabolic pathways during pork storage. Analysis of variance (ANOVA) identified 873 differential metabolites, with key metabolic pathways being amino acid metabolism and nucleotide metabolism. The random forest regression model screened 40 potential marker compounds, highlighting the role of pentose-related metabolism in pork spoilage. Multiple linear regression analysis revealed D-xylose, xanthine, and pyruvaldehyde as key marker compounds related to the freshness of refrigerated pork.
Article
Chemistry, Applied
Caiyan Huang, Dequan Zhang, Zhenyu Wang, Yingxin Zhao, Christophe Blecker, Shaobo Li, Xiaochun Zheng, Li Chen
Summary: This study investigated the potential of 8 biomarkers (PGK1, PKM2, PGM1, ENO3, MYBPC1, MYLPF, TNNC1, and TNNI1) to characterize lamb meat quality based on their abundance and enzymatic activity. Different meat quality groups (QF and LT muscles) were sampled from 100 lamb carcasses at 24 h postmortem. The relative abundance of PKM2, PGK1, PGM1, ENO3, MYBPC1, MYLPF, and TNNI1 was significantly different between LT and QF muscle groups (P < 0.01), and the activity of PKM, PGK, PGM, and ENO was lower in the LT group compared to the QF group (P < 0.05). These findings indicate that these biomarkers can serve as robust indicators of lamb meat quality and provide insights into the molecular mechanisms of meat quality formation.
Article
Food Science & Technology
Le Xu, Chunyou Liu, Shaobo Li, Jinrong Xu, Huan Liu, Xiaochun Zheng, Dequan Zhang, Li Chen
Summary: This investigation aims to elucidate the roles of lipids on the volatilome evolution of postmortem lamb and its possible modulated mechanism behind. Chilled storage improved tenderness of muscle tissue and induced color variation of lamb. Differential aroma-active compounds were identified in different stages of storage, and discriminant analysis recognized the differential lipid species corresponding to different stages of lamb flavor development, including phospholipids, sphingolipids, glycerolipids and fatty acyls.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Applied
Can Xiang, Shaobo Li, Dequan Zhang, Caiyan Huang, Yingxin Zhao, Xiaochun Zheng, Zhenyu Wang, Li Chen
Summary: This study analyzed the flavor profile of six commercially available Chinese indigenous breeds of sheep meat and successfully distinguished them based on their flavor characteristics. Various techniques were used, such as GC-MS, E-nose, HPLC, and E-tongue, to analyze the volatile and non-volatile compounds in the meat samples. The results showed that specific compounds and sensor responses played important roles in differentiating between sheep breeds.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Food Science & Technology
Yuanyuan Su, Cheng Li, Xiafei Huo, Dequan Zhang, Xinxin Zhao, Shaobo Li, Li Chen, Xiaochun Zheng
Summary: Spoilage indicator films are widely used in food safety to detect the freshness of food through color change. In this study, a novel spoilage indicator film was developed using polyvinyl alcohol (PVA)/cassava starch (CS) and neutral red (NR) as a pH-sensitive indicator. The addition of NR greatly improved the compatibility of the film, as confirmed by microstructural analysis and Fourier transform infrared spectroscopy. The film was attached to the inner package to avoid direct contact with the meat, and it successfully monitored the spoilage of meat over time. Color changes from dark red to orange were observed, and a polynomial model was constructed to quantify the freshness of pork, mutton, and chicken during storage based on the color difference. The developed system shows great potential for freshness monitoring in food packaging.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2023)