Evaluation of Total Phenolic Compound and Cytotoxic Activity of Clinacanthus nutans

Clinacanthus nutans leaves were extracted in distilled water at 0.5, 1, 3, 5 and 24 h. The plant extracts were determined the highest amount of total phenolic compound and used for evaluating the cytotoxicity test. The median (LC50) and 90% lethal concentration (LC90) against Artemia salina at varied concentrations of this plant extractions as 0, 400, 800, 1600, 3200, 6400 and 12800 mu g/ml were evaluated within 24 h exposure. The result revealed that the total phenolic compound measurements in each time extraction were 34.68 +/- 3.17, 46.71 +/- 9.31, 36.13 +/- 7.06, 26.53 +/- 8.83 and 30.15 +/- 3.09 mg gallic acid equivalent per g of extract, respectively. Due to the highest amount of total phenolic compound, the 1 h aqueous extract of C. nutans leaf expressed the 24 h LC50 and LC90 values against A. salina were 7,151.1 and 12,008.7 mu g/ml, respectively.