Article
Virology
Geon-Woo Kim, Aleem Siddiqui
Summary: This study identifies an additional m6A site in the HBV genome at nt 1616, which negatively regulates HBx RNA and protein expression. In the absence of m6A methyltransferases (METTL3/14) and the reader protein (YTHDF2), HBx RNA and protein expression levels increase. Mutations in the m6A sites in the HBx coding region also lead to increased HBx RNA and protein expression. These findings reveal the unique positional effects of m6A modification on the regulation of HBx protein expression.
JOURNAL OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Geon-Woo Kim, Aleem Siddiqui
Summary: This study reveals that the viral protein HBV X (HBx) is responsible for the m(6)A modification of viral transcripts, with evidence showing its interaction with m(6)A methyltransferase complexes on the HBV minichromosome. The role of HBx extends to host phosphatase and tensin homolog (PTEN) mRNA, providing insight into how a viral protein recruits RNA methylation machinery to modify RNAs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Geon-Woo Kim, Aleem Siddiqui
Summary: Studies have shown that N6-methyladenosine (m(6)A)) modification plays a crucial role in hepatitis virus RNA, affecting viral replication and liver disease pathogenesis. These modifications may also impact m(6)A modifications in host cellular RNA, influencing gene expression and immunity.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Review
Cell Biology
Qianyu Feng, Hongwei Zhao, Lili Xu, Zhengde Xie
Summary: N6-methyladenosine modification is a crucial RNA modification in eukaryotes, especially in relation to diseases caused by viruses. Studies have shown that m(6)A modification plays diverse regulatory roles in the life cycle of viruses.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Microbiology
Baoxin Zhao, Weijie Wang, Yan Zhao, Hongxiu Qiao, Zhiyun Gao, Xia Chuai
Summary: The m(6)A modification plays a regulatory role in innate and adaptive immune responses during viral infections, affecting processes such as interferon response, immune cell differentiation, and inflammatory cytokine production.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jianzi Lan, Bowen Xu, Xin Shi, Qi Pan, Qing Tao
Summary: In diabetic nephropathy, the WTAP gene promotes the m(6)A methylation of NLRP3 mRNA, leading to upregulation of NLRP3 inflammasome activation and subsequent cell pyroptosis and inflammation.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2022)
Article
Entomology
Yaqing Chen, Yushan Lai, Runzhou Liu, Lin Yao, Xiao-Qiang Yu, Xiaoyun Wang
Summary: In this study, the role of RNA m(6)A epigenetic regulation in the embryonic development of Spodoptera frugiperda was analyzed. The highly conserved function of m(6)A methyltransferases SfrMETTL3 and SfrMETTL14 among insects and mammals was discovered. Transcriptome-wide analysis revealed differential expression of m(6)A-modified transcripts in 2-hour and 24-hour embryos, with enrichment in genes related to cell and organ development. Moreover, it was found that RNA m(6)A methylation may regulate the transcriptional levels of genes related to tissue and organ development during the embryonic stage. This study provides new insights into the embryonic development of insects.
Article
Medicine, General & Internal
Tong Zhao, Jianni Qi, Tiantian Liu, Hao Wu, Qiang Zhu
Summary: This study found that N6-methyladenosine modification is closely related to immune cell infiltration and fibrosis stage in chronic HBV-infected liver tissue. Evaluating the m6A modification pattern and immune infiltration characteristics can provide a better understanding of the progression of liver cirrhosis.
FRONTIERS IN MEDICINE
(2022)
Article
Neurosciences
Lin Zhang, Jian Xia
Summary: This review summarizes the relationship between m6A modification and cell apoptosis, particularly its role in the nervous system, and analyzes the limitations of current research.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Cell Biology
Ping Wu, Xing Fang, Yalan Liu, Yaoyun Tang, Wei Wang, Xin Li, Yuhua Fan
Summary: This study found that circRNA circCUX1 is upregulated in patients with HPSCC, mediates radiotherapy resistance through m6A methylation, and affects radiotherapy efficacy by inhibiting the release of inflammatory factors.
CELL DEATH & DISEASE
(2021)
Review
Cell Biology
Ganggang Zhai, Likang Xiao, Chenyang Jiang, Songkai Yue, Meng Zhang, Jia Zheng, Zeming Liu, Yonghui Dong
Summary: Recent studies have shown the importance of m6A modification in the development of osteoarthritis, indicating its potential significance for the treatment of OA.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Min Li, Xuan Zha, Shengjun Wang
Summary: The widely studied N6-methyladenosine (m6A) modification of mRNA has shown conflicting effects on tumor progression. In addition to its impact on tumor cells, m6A mRNA also plays a crucial role in stromal cells within the tumor microenvironment.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2021)
Review
Cell Biology
Jiahui Luo, Tao Xu, Kai Sun
Summary: m6A modification plays a crucial role in regulating post-transcriptional expression of target genes and is associated with various inflammatory states, including autoimmunity, infection, metabolic disease, cancer, neurodegenerative diseases, heart diseases, and bone diseases. Despite the significant potential of m6A as a biomarker and therapeutic target for inflammatory diseases, systematic knowledge of the relationship between m6A modification and inflammation in human diseases remains unclear.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Microbiology
Hao Su, Henley Cheung, Harry Cheuk-Hay Lau, Hongyan Chen, Xiaoting Zhang, Na Qin, Yifei Wang, Matthew Tak Vai Chan, William Ka Kei Wu, Huarong Chen
Summary: Understanding the interaction between gut microbiota and host RNA m6A modification provides valuable insights into the mechanisms and therapeutic strategies for cancer. The gut microbiota plays a crucial role in regulating host functions and has a significant impact on human health. Dysbiosis of the gut microbiota is associated with various diseases including cancer. The gut microbiota can influence cancer development through mechanisms such as cell regulation, immune response, and metabolic changes. Aberrant RNA m6A modifications have been shown to play a critical role in cancer development. This review aims to summarize the roles of gut microbiota and RNA m6A modifications in cancer and their potential interactions.
FEMS MICROBIOLOGY REVIEWS
(2023)
Article
Cell Biology
Fabian Figueroa, Alonso Vega-Gibson, Joseline Catrileo, Aracelly Gaete-Argel, Sebastian Riquelme-Barrios, Luis Antonio Alonso-Palomares, Lorena Tapia, Fernando Valiente-Echeverria, Ricardo Soto-Rifo, Monica L. Acevedo
Summary: The N-6-methyladenosine modification negatively affects HRSV replication by interfering with the assembly of inclusion bodies which are the main factories for viral genomic RNA synthesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
