Article
Virology
Mamata Panigrahi, Patricia A. Thibault, Joyce A. Wilson
Summary: miR-122 plays a crucial role in the replication and infection of hepatitis C virus (HCV). It functions primarily to initiate HCV replication and supports the formation of new replication complexes during ongoing infection and after infected cell division.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Yujiao He, Maolin Huang, Chunyan Tang, Yan Yue, Xiao Liu, Zhebin Zheng, Hongbo Dong, Deming Liu
Summary: MicroRNA-122 plays a critical role in HCV replication, and daidzein inhibits HCV replication by decreasing miR-122 levels, enhancing the antiviral effect of IFN-?, without significantly affecting cell growth.
Review
Microbiology
Mamata Panigrahi, Michael A. Palmer, Joyce A. Wilson
Summary: This review explores the unique relationship between liver-specific microRNA miR-122 and Hepatitis C virus (HCV), as well as the role of miR-122 in the virus life cycle, viral tropism, and pathogenesis. The review also discusses the impact of anti-miR-122 therapy on viral replication and how viruses manipulate host factors during the initial stage of infection to establish a successful infection.
Article
Biochemistry & Molecular Biology
Zuben P. Brown, Irina S. Abaeva, Swastik De, Christopher U. T. Hellen, Tatyana Pestova, Joachim Frank
Summary: This study presents cryo-EM structures of hepatitis C virus IRES initiation complexes, revealing the dynamic network of 40S/IRES contacts and the conformational changes during the transition from eIF2- to eIF5B-containing 48S complexes, providing important insights into the process of subunit joining.
Article
Genetics & Heredity
Lucas dos Santos da Silva, William Borges Domingues, Bruna Fagundes Barreto, Amanda Weege da Silveira Martins, Eduardo Nunes Dellagostin, Eliza Rossi Komninou, Carine D. Corcini, Antonio Sergio Varela Junior, Vinicius Farias Campos
Summary: Electroporation using capillary technique on high-quality bull sperm increases internalization of exogenous DNA without affecting mitochondrial activity, leading to reduced sperm motility. Expression of miRNA-122-5p is upregulated in sperm electroporated at 1500 V, and the presence of exogenous DNA does not impact its expression.
Article
Endocrinology & Metabolism
Yujin Ding, Guangdi Li, Zhiguang Zhou, Tuo Deng
Summary: Diabetes and hepatitis C are two distinct diseases, but they are closely related, as hepatitis C virus infection may lead to diabetes and influence the occurrence and development of diabetes through multiple mechanisms.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2021)
Article
Virology
Mamata Panigrahi, Michael A. A. Palmer, Joyce A. A. Wilson
Summary: The 5 ' untranslated region (UTR) of the hepatitis C virus (HCV) genome regulates virus replication and translation. Binding of miR-122 to the 5 ' UTR regulates viral replication and translation, but its precise mechanism is still unresolved. Our study shows that miR-122 binding stimulates viral translation and that enhancing translation and stabilizing the viral genome can rescue miR-122-independent HCV replication. This suggests that miR-122 plays a primary role in promoting HCV translation and genome stabilization.
JOURNAL OF VIROLOGY
(2023)
Article
Gastroenterology & Hepatology
Sahar Tabatabavakili, Wesam Aleyadeh, Orlando Cerrocchi, Harry L. A. Janssen, Bettina E. Hansen, Isaac I. Bogoch, Jordan J. Feld
Summary: Sexual transmission of HCV is uncommon in HIV-negative MSM PrEP users, but there are higher rates of other STIs. Routine risk-based HCV surveillance and periodic STI monitoring are recommended for PrEP users.
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Review
Microbiology
Carla E. Gallardo-Flores, Che C. Colpitts
Summary: Cyclophilins play important roles in HCV infection, with cyclophilin inhibitor therapy showing effectiveness in reducing viral replication. Recently, cyclophilins have also been identified as host factors for flavivirus infection. Further research is needed to clarify the roles of cyclophilins in flavivirus replication.
Article
Biochemistry & Molecular Biology
Marzieh Jamalidoust, Maryam Shafaati, Mehdi Kalani, Maryam Zare, Mazyar Ziyeayan
Summary: The study demonstrates the inhibitory effect of let-7b on HCV replication by promoting apoptosis and reducing HCV-RNA load. Therefore, let-7b may serve as a potential antiviral therapy target.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Genetics & Heredity
Amin Ullah, Irshad Ur Rehman, Katharina Ommer, Nadeem Ahmed, Margarete Odenthal, Xiaojie Yu, Jamshaid Ahmad, Tariq Nadeem, Qurban Ali, Bashir Ahmad
Summary: This study evaluated the expression profile of circulating miR-192 and 29a and found that they were significantly upregulated in patients with chronic HCV infection, suggesting their potential as biomarkers for hepatic disease.
Review
Gastroenterology & Hepatology
Alessandra Mangia, Rosa Cotugno, Giovanna Cocomazzi, Maria Maddalena Squillante, Valeria Piazzolla
Summary: The World Health Organization's goal of eliminating Hepatitis C virus (HCV) by 2030 using direct-acting antiviral treatments has become less attainable post the 2020 pandemic of coronavirus disease 2019. Therefore, a more realistic approach of micro-elimination in population segments where targeted strategies of prevention and treatment are easily attained has been promoted in Europe. Active involvement of stakeholders and community participation are essential components for the success of a micro-elimination project tailored at the geographical level according to HCV epidemiology and individual country's income.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Gastroenterology & Hepatology
Hirohito Yoneyama, Asahiro Morishita, Hisakazu Iwama, Koji Fujita, Takahiro Masaki, Joji Tani, Tomoko Tadokoro, Takako Nomura, Teppei Sakamoto, Kyoko Oura, Kei Takuma, Mai Nakahara, Shima Mimura, Akihiro Deguchi, Makoto Oryu, Kunihiko Tsutsui, Takashi Himoto, Kunitada Shimotohno, Takaji Wakita, Hideki Kobara, Tsutomu Masaki
Summary: The study found that hsa-miR-762 may be one of the host factors involved in the exclusion of HCV through DAA therapy. Its expression quantiles significantly increased when HCV was eradicated, and decreased in cases of treatment failure.
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Public, Environmental & Occupational Health
Daniel O'Keefe, Keo Samley, Voeurng Bunreth, Tonia Marquardt, Serge Eric Bobi, Kien Antharo, Chanroeun San Kim, Hem Sothy, Thoang Sokha, Chor Samnang, Yan Sokchea, Farah Hossain, Suna Balkan, Mickael Le Paih, Jean-Philippe Dousset
Summary: A nurse-led model of care in a resource-poor setting in rural Cambodia was able to provide safe and effective diagnosis and treatment for patients with HCV infections. The pilot project achieved a 100% treatment completion rate and a 94% sustained virological response rate.
BULLETIN OF THE WORLD HEALTH ORGANIZATION
(2023)
Article
Biophysics
Ru Cheng, Fu Zhu, Min Huang, Qiang Zhang, Hui Hong Yan, Xiao Hui Zhao, Fu Kang Luo, Chun Mei Li, Hui Liu, Gao Lin Liang, Cheng Zhi Huang, Jian Wang
Summary: In this study, a new biosensor named hepatitis virus indicator was developed for the simultaneous detection of HBV and HCV. The biosensor utilized Exo III assisted signal amplification strategy and particle counting technology with a dark-field microscopy, enabling sensitive and selective detection.
BIOSENSORS & BIOELECTRONICS
(2022)