期刊
INDIAN JOURNAL OF MICROBIOLOGY
卷 57, 期 2, 页码 211-217出版社
SPRINGER
DOI: 10.1007/s12088-016-0632-2
关键词
Gamma-aminobutyric acid (GABA); Glutamate decarboxylase (gad) gene; Kung-Som; Lactic acid bacteria; Lactobacillus futsaii
资金
- Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program [PHD/0060/2556]
- Shell Centennial Education Fund, Shell Companies in Thailand
- Prince of Songkla University [AGR580355S]
Kung-Som is a popular traditional Thai fermented shrimp product. It is rich in glutamic acid, which is the major substrate for the biosynthesis of gamma-aminobutyric acid (GABA) by lactic acid bacteria (LAB). In the present study, LAB from Kung-Som were isolated, screened for GABA formation, and the two isolates that transform glutamic acid most efficiently into GABA were identified. Based on the API-CHL50 fermentation profile and a phylogenetic tree of 16S rDNA sequences, strain CS3 and CS5 were identified as Lactobacillus futsaii, which was for the first time shown to be a promising GABA producer. L. futsaii CS3 was the most efficient microorganism for the conversion of 25 mg/mL monosodium glutamate (MSG) to GABA, with a maximum yield of more than 99% conversion rate within 72 h. The open reading frame (ORF) of the glutamate decarboxylase (gad) gene was identified by PCR. It consists of 1410 bp encoding a polypeptide of 469 amino acids with a predicted molecular weight of 53.64 kDa and an isoelectric point (pI) of 5.56. Moreover, a good quality of the constructed model of L. futsaii CS3 was also estimated. Our results indicate that L. futsaii CS3 could be of interest for the production of GABA-enriched foods by fermentation and for other value-added products.
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