期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 223, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2021.112279
关键词
Pyrene; Chemosensor; Zinc; ATP sensor; Imaging
资金
- National Natural Science Foundation of China [21801064]
A novel highly selective and sensitive turn-on fluorescent chemosensor PCE has been developed for the recognition of Zn2+, displaying remarkable fluorescent enhancement in aqueous acetonitrile solution. The PCE-Zn ensemble can be utilized for monitoring ATP in live cells with selective fluorescence enhancement.
A novel highly selective and sensitive turn-on fluorescent chemosensor PCE to recognize Zn2+ has been developed. The sensor PCE displays a remarkable fluorescent enhancement at 456 nm (lambda(ex) = 340 nm) with Zn2+ without the interference of other biologically important relevant metal ions in aqueous acetonitrile solution. Job's plot and mass spectral studies divulge such the interaction of PCE by Zn2+ was 1:1 binding stoichiometry. The association constant and detection limit of PCE to recognize Zn2+ was found to be 0.948 x 10(4) M-1 and 4.82 x 10(-7) M respectively. The nature of turn-on fluorescence sensor was supported by TD-DFT calculations. And the synthesized probe PCE was able to image intracellular Zn2+ in living cells using confocal imaging techniques. PCE-Zn ensemble showed the remarkable fluorescence enhancement with ATP selectively among other biologically important phosphates. P-31 NMR experiments suggesting that the triphosphates unit of ATP is intact with the PCE-Zn. PCE-Zn ensemble can be utilized for monitoring ATP in live cells.
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