Review
Biochemistry & Molecular Biology
Kaitryn E. Ronning, Paul-Alexandre Dechelle-Marquet, Yueshen Che, Xavier Guillonneau, Florian Sennlaub, Cecile Delarasse
Summary: Alzheimer's disease (AD) is characterized by impaired memory, amyloid plaques, and neurofibrillary tangles. The release of ATP and the upregulation of P2X7 receptor in AD patients contribute to the disease's pathological processes. Various pathways involving P2X7 have been identified to influence the development of AD, including modulation of Aβ plaques, Tau protein lesions, and inflammatory response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Bernadett Iring-Varga, Maria Baranyi, Flora Goloncser, Pal Tod, Beata Sperlagh
Summary: This study investigated the effects of zinc intake changes on depression-like behaviors in mice and found that the antidepressant effect of zinc is partially mediated by the P2X7 receptor.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Carolina Flores-Munoz, Jaime Maripillan, Jacqueline Vasquez-Navarrete, Joel Novoa-Molina, Ricardo Ceriani, Helmuth A. Sanchez, Ana C. Abbott, Caroline Weinstein-Oppenheimer, Donald I. Brown, Ana Maria Cardenas, Isaac E. Garcia, Agustin D. Martinez
Summary: The study revealed that Panx1 channels play a crucial role in human dermal fibroblast migration during wound healing, with their absence or inhibition accelerating cell migration and promoting actin redistribution. This occurs through the release of ATP to the extracellular space and activation of the purinergic receptor P2X7.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Fabio Grassi, Gaia Salina
Summary: This review examines the existing literature on the activity of the P2X7 receptor in autoimmunity, highlighting the different functions in cells involved in immunopathological processes, which make it difficult to model as a druggable target.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Jonathon Smith, Aida Menendez Mendez, Mariana Alves, Alberto Parras, Giorgia Conte, Anindya Bhattacharya, Marc Ceusters, Annette Nicke, David C. Henshall, Eva M. Jimenez-Mateos, Tobias Engel
Summary: P2X7 receptor (P2X7R) plays an important role in neonatal seizures, with its deletion reducing seizure severity and overexpression exacerbating seizure severity. P2X7R deficiency leads to an anti-inflammatory phenotype in microglia, and treatment with a P2X7R antagonist can reduce long-lasting brain hyperexcitability. Targeting P2X7R may represent a novel therapeutic strategy for neonatal seizures.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jens D. Mikkelsen, Sanjay S. Aripaka, Sif Kaad, Burcu A. Pazarlar, Lars Pinborg, Bente Finsen, Andrea Varrone, Benny Bang-Andersen, Jesper F. Bastlund
Summary: A newly developed radioligand [JNJ-64413739] that selectively binds to the purinergic receptor P2X7R has been found to detect neuroinflammation in the brain. The binding properties of JNJ-64413739 in human brain tissue were investigated, and it was found to have different binding abilities in gray and white matter. These findings demonstrate that JNJ-64413739 is suitable for evaluating the distribution and expression of P2X7R in the human brain.
ACS CHEMICAL NEUROSCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Ronald Sluyter, Sahil Adriouch, Stephen J. Fuller, Annette Nicke, Reece A. Sophocleous, Debbie Watson
Summary: The P2X7 receptor is a ligand-gated cation channel that is activated by extracellular adenosine 5'-triphosphate. Animal models have played a crucial role in understanding the physiological and pathophysiological functions of P2X7 in human health and disease. This article highlights the contributions of animal models in generating tools for studying P2X7, such as specific antibodies, recombinant receptors, and pharmacokinetic testing of P2X7 antagonists. It also discusses the use of mouse, rat, and other animal models in studying P2X7, including disease models, knockout mice, reporter mice, and humanized mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Franca Rosa Guerini, Cristina Agliardi, Elisabetta Bolognesi, Milena Zanzottera, Domenico Caputo, Maria Barbara Pasanisi, Marco Rovaris, Mario Clerici
Summary: Multiple sclerosis (MS) is a chronic autoimmune inflammatory disease of the central nervous system. This study finds that two P2X7 receptor polymorphisms (348Thr and 464Arg) are associated with disease severity in patients with relapsing remitting multiple sclerosis (RRMS).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Peter Cuthbertson, Amal Elhage, Dena Al-Rifai, Reece A. Sophocleous, Ross J. Turner, Ashraf Aboelela, Hiwa Majed, Richard S. Bujaroski, Iman Jalilian, Michael J. Kelso, Debbie Watson, Benjamin J. Buckley, Ronald Sluyter
Summary: 6-FPHMA is identified as a novel P2X7 antagonist that can inhibit P2X7 from multiple species. However, its poor selectivity excludes it from being used as a specific P2X7 antagonist.
Review
Neurosciences
Kevin Carvalho, Elodie Martin, Aurelia Ces, Nadege Sarrazin, Pauline Lagouge-Roussey, Caroline Nous, Leyna Boucherit, Ihsen Youssef, Annick Prigent, Emilie Faivre, Sabiha Eddarkaoui, Thibaut Gauvrit, Didier Vieau, Susana Boluda, Vincent Huin, Bertrand Fontaine, Luc Buee, Benoit Delatour, Patrick Dutar, Florian Sennlaub, Xavier Guillonneau, David Blum, Cecile Delarasse
Summary: The purinergic receptor P2X7 plays a crucial role in Alzheimer's disease and Tauopathies, with deletion of P2X7 having significant effects on inflammatory mediators and memory function in Tau pathology. P2X7 inhibitors may be ideal drugs for treating these neurodegenerative diseases.
PROGRESS IN NEUROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kate Dunning, Adeline Martz, Francisco Andres Peralta, Federico Cevoli, Eric Boue-Grabot, Vincent Compan, Fanny Gautherat, Patrick Wolf, Thierry Chataigneau, Thomas Grutter
Summary: Activation of P2X7 receptors leads to various physiological responses, such as the secretion of pro-inflammatory cytokines and modulation of cell death. Current facilitation and macropore formation involve functional complexes that increase P2X7 open probability and mediate large molecule permeation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Bartosz Szymczak, Joanna Czarnecka, Sylwia Czach, Wieslaw Nowak, Katarzyna Roszek
Summary: This study reveals the impact of the interaction between purinergic signaling and temozolomide (TMZ) on human glioma cell line, including changes in cell proliferation, viability, and migratory ability. The results demonstrate that the combined action of ATP and TMZ significantly reduces the viability and migration ability of differentiating A172 glioma cells. These findings suggest that the concentration of ATP in the glioma cell microenvironment needs to be maintained at an appropriate level to enhance the anti-cancer activity of TMZ.
CELLULAR SIGNALLING
(2023)
Review
Biochemistry & Molecular Biology
Tobias Engel
Summary: Epilepsy is a brain disease affecting over 70 million people worldwide, characterized by recurrent spontaneous seizures. Challenges in epilepsy management include diagnosis and treatment. Current research aims to identify new drugs with different mechanisms of action for patients not responding to current medications. The P2X7 receptor (P2X7R) has attracted attention as a potential therapeutic and diagnostic target due to its involvement in epilepsy and altered expression in experimental models and patients. This review discusses the latest findings and the potential of P2X7R-based treatments for epilepsy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physiology
Elizabeth A. Townsend, Arturo Guadarrama, Lei Shi, Elon Roti Roti, Loren C. Denlinger
Summary: This study found that stimulation of P2X(7) in alveolar macrophages of patients with mild-to-moderate asthma produces eicosanoids associated with airway inflammation and resolution. The responses are partially regulated by P2X7 pore function.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Article
Cell Biology
Yin Yang, Meaghan E. Story, Xingxing Hao, Tina L. Sumpter, Alicia R. Mathers
Summary: This study investigates the role of P2X7R signaling in Th17 and Th1 cell differentiation. The results show that P2X7R blockade does not affect Th17 and Th1 development in vivo, but in vitro experiments suggest that P2X7R agonist treatment of DCs can enhance Th17 differentiation. Additionally, the expression of P2X7R on DCs and CD4(+) T cells has an impact on Th1 responses.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Larissa Mattos Feijo, Viviane Abreu de Andrade, Robson Coutinho-Silva
Summary: The digestive system is an important topic in biological science teaching, but students' knowledge on the topic often does not meet recommended levels. A study has shown that a practical activity can help students reconstruct accurate concepts of the digestive system and overcome epistemological obstacles.
JOURNAL OF BIOLOGICAL EDUCATION
(2022)
Article
Cell Biology
Jessica A. P. Oliveira, Mariana Gandini, Jorgenilce S. Sales, Sergio K. Fujimori, Mayara G. M. Barbosa, Valber S. Frutuoso, Milton O. Moraes, Euzenir N. Sarno, Maria C. Pessolani, Roberta O. Pinheiro
Summary: The study revealed that IDO-1 enzyme in human monocyte-derived dendritic cells can be differentially regulated by Mycobacterium leprae and its fractions through the activation of the TLR2 pathway. MLSA may increase Treg cell frequency, while MLMA can induce proinflammatory and regulatory cytokines in the cells.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Article
Virology
Debora Familiar-Macedo, Iury Amancio Paiva, Jessica Badolato-Correa da Silva, Fabiana Rabe de Carvalho, Helver Goncalves Dias, Alex Pauvolid-Correa, Caroline Fernandes dos Santos, Mariana Gandini, Andrea Alice Silva, Silvia Maria Baeta Cavalcanti, Solange Artimos de Oliveira, Renata Artimos de Oliveira Vianna, Elzinandes Leal de Azeredo, Alba Grifoni, Alessandro Sette, Daniela Weiskopf, Claudete Aparecida Araujo Cardoso, Luzia Maria de-Oliveira-Pinto
Summary: This study investigated how the immune response to ZIKV during pregnancy affects the clinical outcome of babies, finding a correlation between the T cell responses of mothers and whether the child was born with congenital Zika syndrome (CZS).
Article
Medicine, Research & Experimental
Tiago Marcon dos Santos, Osmar Vieira Ramires Junior, Vinicius Santos Alves, Robson Coutinho-Silva, Luiz Eduardo Baggio Savio, Angela T. S. Wyse
Summary: Chronic mild hyperhomocysteinemia in rats affects oxidative stress and gene expression in the striatum and cerebellum, but does not impair motor function.
Review
Biochemistry & Molecular Biology
Mariana Chaves, Luiz Eduardo Savio, Robson Coutinho-Silva
Summary: Leishmaniasis is a neglected tropical disease that poses a public health problem in developing countries. The interaction between Leishmania parasites, extracellular nucleotides, and purinergic receptors plays a crucial role in determining disease control or dissemination.
BIOMEDICAL JOURNAL
(2022)
Article
Neurosciences
Josiane Silva Silveira, Osmar Vieira Ramires Junior, Felipe Schmitz, Fernanda Silva Ferreira, Fabiana Cristina Rodrigues, Robson Coutinho Silva, Luiz Eduardo Baggio Savio, Angela T. S. Wyse
Summary: Supplementing with 4 mg/kg of folic acid during pregnancy can negatively affect the behavior and biochemical indicators of offspring.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Immunology
Stephanie Alexia Cristina Silva Santos, Pedro Muanis Persechini, Bianca Monteiro Henriques-Santos, Victoria Gabriela Bello-Santos, Newton G. Castro, Julia Costa de Sousa, Fernando Ariel Genta, Marcelo Felippe Santiago, Robson Coutinho-Silva, Luiz Eduardo Baggio Savio, Eleonora Kurtenbach
Summary: The activation of P2X7 receptor in immune cells is associated with lysosomal dysfunction and leakage. This study reveals that the P2X7 receptor and pannexin-1 channels mediate calcium influx, leading to the activation of specific ion channels on the membranes of endosomes and lysosomes, resulting in lysosomal dysfunction and cathepsin release.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Claudio Bernardazzi, Morgana Teixeira Lima Castelo-Branco, Beatriz Pego, Beatriz Elias Ribeiro, Siane Lopes Bittencourt Rosas, Patricia Teixeira Santana, Joao Carlos Machado, Camille Leal, Fabiano Thompson, Robson Coutinho-Silva, Heitor Siffert Pereira de Souza
Summary: P2X7 receptor plays an important role in the development of colitis-associated colorectal cancer by activating intracellular signaling pathways and the inflammasome, enhancing inflammatory response and promoting tumor formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Osmar Vieira Ramires Junior, Tiago Marcon dos Santos, Josiane Silva Silveira, Raissa Leite-Aguiar, Robson Coutinho-Silva, Luiz Eduardo Baggio Savio, Angela T. S. Wyse
Summary: This study evaluated the effects of mild hyperhomocysteinemia on behavior and neurochemical parameters in male rats, and investigated the therapeutic effects of ibuprofen and rivastigmine. The results showed that mild hyperhomocysteinemia caused cognitive deficits and impaired motor coordination, but treatment with ibuprofen and rivastigmine reduced these damages.
MOLECULAR NEUROBIOLOGY
(2022)
Review
Virology
Caroline Fernandes-Santos, Elzinandes Leal de Azeredo
Summary: Dengue is a mosquito-borne viral disease caused by the dengue virus, with a wide range of clinical manifestations. In the innate antiviral response, Toll-like receptors (TLRs) play a critical role in pathogen recognition and the production of inflammatory cytokines and interferons essential for preventing viral replication.
Review
Microbiology
Natasha Cabral, Vilma de Figueiredo, Mariana Gandini, Cintia Fernandes de Souza, Rychelle Affonso Medeiros, Leticia Miranda Santos Lery, Flavio Alves Lara, Cristiana Santos de Macedo, Maria Cristina Vidal Pessolani, Geraldo Moura Batista Pereira
Summary: This article discusses the possible initial steps of Mycobacterium leprae infection that may lead to the onset of leprosy, focusing on events before the manifestation of the established clinical forms. The study suggests that Mycobacterium leprae can survive in the initial phase of infection through interactions with skin-resident macrophages and Schwann cells of peripheral nerves. Furthermore, these interactions induce metabolic changes and differentiation of specific T cells, facilitating the progression of the infection to active disease.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Thiara Manuele Alves de Souza, Caroline Fernandes-Santos, Jessica Araujo da Paixao de Oliveira, Larissa Cristina Teixeira Tome, Victor Edgar Fiestas-Solorzano, Priscila Conrado Guerra Nunes, Gabriel Macedo Costa Guimaraes, Juan Camilo Sanchez-Arcila, Iury Amancio Paiva, Luis Jose de Souza, Paulo Vieira Damasco, Valber da Silva Frutuoso, Manoela Heringer, Luzia Maria De Oliveira-Pinto, Roberta Olmo Pinheiro, Flavia Barreto dos Santos, Elzinandes Leal de Azeredo
Summary: This study evaluated the activity of IDO-1 and the patterns of cytokines/chemokines in CHIKV patients. It was found that IDO-1 activity increased during the early acute phase of infection and decreased in the chronic phase. In the acute phase, concentrations of TNF-alpha, IL-6, IFN-gamma, CCL2/MCP-1, and CXCL10/IP-10 were increased. In the chronic phase, concentrations of CCL4/MIP-1 beta were increased, especially in patients with arthritis.
Article
Medicine, General & Internal
Thabatta Leal Silveira Andrezo Rosa, Mayara Abud Mendes, Natasha Ribeiro Cardoso Linhares, Thais Fernanda Rodrigues, Andre Alves Dias, Thyago Leal-Calvo, Mariana Gandini, Helen Ferreira, Fabricio da Mota Ramalho Costa, Anna Maria Sales, Thais Porto Amadeu, Veronica Schmitz, Roberta Olmo Pinheiro, Luciana Silva Rodrigues, Milton Ozorio Moraes, Maria Cristina Vidal Pessolani
Summary: This study investigated the activation of the type I interferon (IFN-I) pathway in leprosy patients with erythema nodosum leprosum (ENL). The results showed higher activation of the IFN-I pathway in ENL patients, which was reduced during thalidomide treatment. Peripheral pDCs were found to be involved in the production of IFN-I during ENL. These findings contribute to the identification of biomarkers for early diagnosis and the discovery of new therapeutic targets for the management of ENL reactions.
FRONTIERS IN MEDICINE
(2022)
Article
Microbiology
Kivia Queiroz De Andrade, Cassio Luiz Coutinho Almeida-da-Silva, David M. Ojcius, Robson Coutinho-Silva
Summary: This study demonstrated the involvement of the P2 x7-caspase-1 and caspase-11 pathways in the immune response against infection by periodontal bacteria, Porphyromonas gingivalis and Fusobacterium nucleatum, respectively.
CURRENT RESEARCH IN MICROBIAL SCIENCES
(2021)
Meeting Abstract
Gastroenterology & Hepatology
Beatriz Elias Ribeiro, Hayandra F. Nanini, Patricia T. Santana, Rodrigo P. de Oliveira, Joao Carlos Machado, Cynthia M. Cascabulho, Morgana T. Castelo-Branco, Robson Coutinho-Silva, Heitor S. De Souza
