The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16

Cupriavidus necator H16 can convert CO2 into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application specific modules. Plasmids were characterised in terms of transmissibility, stability, copy number and compatibility.

Automated summary

The study focused on engineering Cupriavidus necator H16 to convert CO2 into industrial chemicals and fuels. Modular plasmids were designed and tested to facilitate this process, with characteristics such as transmissibility, stability, copy number, and compatibility being assessed.