Sensitive glucose biosensor based on cyclodextrin modified carbon nanotubes for detecting glucose in honey

As a healthy and nutritious food, the taste of honey is usually affected by glucose crystals. To investigate the glucose concentration of honey, a new electroanalytical method based on a composite material of functionalized MWCNTs with beta-cyclodextrin (beta-CD/MWCNTs) was hereby proposed. After glucose oxidase (GOx) loading on beta-CD/MWCNTs, a new biosensor GOx/beta-CD/MWCNTs/GCE was constructed for the detection of glucose in honey. Scanning electron microscopy (SEM) and infrared spectroscopy (FT-IR) proved the successful immobi-lization of GOx on the beta-CD/MWCNTs carrier. The prepared biosensor showed a linear response to the glucose concentration in the range of 50 mu M similar to 1.15 mM with the detection limit of 0.42 mu M and a sensitivity of 32.28 mu A mM(-1) cm(-2) was obtained. The biosensor has excellent stability (95.7 % after two weeks), reproducibility (n = 10, relative standard deviation (RSD) = 2.5 %) and anti-interference. HPLC results validated the accuracy of the newly proposed method in the detection of glucose in real honey samples.

Automated summary

A new electroanalytical method based on a composite material of functionalized multi-walled carbon nanotubes with beta-cyclodextrin was proposed for detecting glucose concentration in honey. The prepared biosensor showed excellent performance, stability, and accuracy in detecting glucose in real honey samples.