A generic sample preparation method for the multiplex analysis of seven therapeutic monoclonal antibodies in human plasma or serum with liquid chromatography-tandem mass spectrometry

Simultaneous quantification of free adalimumab and infliximab in human plasma using a target-based sample purification and liquid chromatography-tandem mass spectrometry.

(2019) Mohsin El Amrani et al.   THERAPEUTIC DRUG MONITORING

Quantification of total dinutuximab concentrations in neuroblastoma patients with liquid chromatography tandem mass spectrometry

(2018) Mohsin El Amrani et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Proteins and antibodies in serum, plasma, and whole blood—size characterization using asymmetrical flow field-flow fractionation (AF4)

(2018) Mats Leeman et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

LC–MS bioanalysis of Trastuzumab and released emtansine using nano-surface and molecular-orientation limited (nSMOL) proteolysis and liquid–liquid partition in plasma of Trastuzumab emtansine-treated breast cancer patients

(2017) Noriko Iwamoto et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

2016 FDA drug approvals

(2017) Asher Mullard   NATURE REVIEWS DRUG DISCOVERY

A simple and rapid LC-MS/MS method for therapeutic drug monitoring of cetuximab: a GPCO-UNICANCER proof of concept study in head-and-neck cancer patients

(2017) François Becher et al.   Scientific Reports

Bioanalysis of bevacizumab and infliximab by high-temperature reversed-phase liquid chromatography with fluorescence detection after immunoaffinity magnetic purification

(2016) Kenichiro Todoroki et al.   ANALYTICA CHIMICA ACTA

Infliximab quantitation in human plasma by liquid chromatography-tandem mass spectrometry: towards a standardization of the methods?

(2016) Jean-Francois Jourdil et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Quantification of active infliximab in human serum with liquid chromatography–tandem mass spectrometry using a tumor necrosis factor alpha -based pre-analytical sample purification and a stable isotopic labeled infliximab bio-similar as internal standard: A target-based, sensitive and cost-effective method

(2016) Mohsin El Amrani et al.   JOURNAL OF CHROMATOGRAPHY A

Quantitative bottom up analysis of infliximab in serum using protein A purification and integrated μLC-electrospray chip IonKey MS/MS technology

(2016) Anne J Kleinnijenhuis et al.   Bioanalysis

Validation of a biotherapeutic immunoaffinity-LC–MS/MS assay in monkey serum: ‘plug-and-play’ across seven molecules

(2016) Surinder Kaur et al.   Bioanalysis

Generic method approaches for monoclonal antibody therapeutics analysis using both ligand binding and LC–MS/MS techniques

(2016) Jean W Lee   Bioanalysis

Quantification of biopharmaceuticals and biomarkers in complex biological matrices: a comparison of liquid chromatography coupled to tandem mass spectrometry and ligand binding assays

(2015) Peter Bults et al.   Expert Review of Proteomics

Quantitation of infliximab using clonotypic peptides and selective reaction monitoring by LC–MS/MS

(2015) Maria Alice V. Willrich et al.   INTERNATIONAL IMMUNOPHARMACOLOGY

Post-pellet-digestion precipitation and solid phase extraction: A practical and efficient workflow to extract surrogate peptides for ultra-high performance liquid chromatography – tandem mass spectrometry bioanalysis of a therapeutic antibody in the low ng/mL range

(2015) Chao Gong et al.   JOURNAL OF CHROMATOGRAPHY A

LC–MS-based quantification of intact proteins: perspective for clinical and bioanalytical applications

(2015) Irene van den Broek et al.   Bioanalysis

Generic Automated Method for Liquid Chromatography–Multiple Reaction Monitoring Mass Spectrometry Based Monoclonal Antibody Quantitation for Preclinical Pharmacokinetic Studies

(2014) Qian Zhang et al.   ANALYTICAL CHEMISTRY

A Novel and Cost Effective Method of Removing Excess Albumin from Plasma/Serum Samples and Its Impacts on LC-MS/MS Bioanalysis of Therapeutic Proteins

(2014) Guowen Liu et al.   ANALYTICAL CHEMISTRY

A versatile method for protein-based antigen bioanalysis in non-clinical pharmacokinetics studies of a human monoclonal antibody drug by an immunoaffinity liquid chromatography–tandem mass spectrometry

(2014) Ichio Onami et al.   JOURNAL OF CHROMATOGRAPHY A

Fully Validated LC-MS/MS Assay for the Simultaneous Quantitation of Coadministered Therapeutic Antibodies in Cynomolgus Monkey Serum

(2013) Hao Jiang et al.   ANALYTICAL CHEMISTRY

Liquid chromatography coupled with tandem mass spectrometry for the bioanalysis of proteins in drug development: Practical considerations in assay development and validation

(2013) Guowen Liu et al.   JOURNAL OF CHROMATOGRAPHY A

Bioanalytical LC–MS/MS of protein-based biopharmaceuticals

(2013) Irene van den Broek et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS I. Sample preparation

(2013) Rainer Bischoff et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Simultaneous Analysis of Multiple Monoclonal Antibody Biotherapeutics by LC-MS/MS Method in Rat Plasma Following Cassette-Dosing

(2012) Hongyan Li et al.   AAPS Journal

General LC-MS/MS Method Approach to Quantify Therapeutic Monoclonal Antibodies Using a Common Whole Antibody Internal Standard with Application to Preclinical Studies

(2012) Hongyan Li et al.   ANALYTICAL CHEMISTRY

Clinical Pharmacokinetic Assessment of an Anti-MAdCAM Monoclonal Antibody Therapeutic by LC-MS/MS

(2012) Mireia Fernández Ocaña et al.   ANALYTICAL CHEMISTRY

Internal standards in the quantitative determination of protein biopharmaceuticals using liquid chromatography coupled to mass spectrometry

(2012) Kees J. Bronsema et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Bioanalytical Approaches to Quantify “Total” and “Free” Therapeutic Antibodies and Their Targets: Technical Challenges and PK/PD Applications Over the Course of Drug Development

(2011) Jean W. Lee et al.   AAPS Journal

Quantitation of a recombinant monoclonal antibody in monkey serum by liquid chromatography–mass spectrometry

(2011) Hongcheng Liu et al.   ANALYTICAL BIOCHEMISTRY

Absolute quantitation of protein therapeutics in biological matrices by enzymatic digestion and LC–MS

(2011) Fumin Li et al.   Bioanalysis

Pellet digestion: a simple and efficient sample preparation technique for LC–MS/MS quantification of large therapeutic proteins in plasma

(2011) Zheng Ouyang et al.   Bioanalysis

Towards Absolute Quantification of Therapeutic Monoclonal Antibody in Serum by LC−MS/MS Using Isotope-Labeled Antibody Standard and Protein Cleavage Isotope Dilution Mass Spectrometry

(2008) Olivier Heudi et al.   ANALYTICAL CHEMISTRY