期刊
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
卷 33, 期 5, 页码 651-667出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09205063.2021.2008788
关键词
Se-methylselenocysteine; polyglycerol dendrimer; cytotoxicity; HNSCC cells line
资金
- National Council for Scientific and Technological Development (CNPq) [307609/2018-9]
Polymeric nanoparticles loaded with selenium (Se) have shown potential in cancer chemotherapy. The functionalization of polyglycerol dendrimer (DPGLy) with seleno-methyl-selenocysteine (SeMeCys) showed promising results in reducing the viability and proliferation of head and neck squamous cell carcinoma (HNSCC) cells, while exhibiting no significant toxicity against Chinese Hamster ovary cell line (CHO-K1).
Polymeric nanoparticles acting as sources of selenium (Se) are currently an interesting topic in cancer chemotherapy. In this study, polyglycerol dendrimer (DPGLy) was functionalized with seleno-methyl-selenocysteine (SeMeCys) by means of Steglich esterification with 4-dimethylaminopyridine/(l-ethyl-3-(3-dimethylaminopropyl)carbodiimide) (EDC/DMAP) and cerium chloride as cocatalyst in acetonitrile at quantitative yields of 98 +/- 1%. The SeMeCys coupling DPGLy efficiency vs. time were determined by Fourier Transform infrared spectroscopy (FTIR) and ultraviolet-visible (UV-Vis) spectroscopy. The cytotoxic effects of SeMeCys-DPGLy on the Chinese Hamster ovary cell line (CHO-K1) and head and neck squamous cell carcinoma (HNSCC) cells line were assessed by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. No signs of general toxicity of SeMeCys-DPGLy against CHO-K1 cells were detectable at which cell viability was greater than 98%. MTS assays revealed that SeMeCys-DPGLy reduced HNSCC cell viability and proliferation at higher doses and long incubation times.
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