Article
Multidisciplinary Sciences
Benjamin C. Orsburn, Yuting Yuan, Namandje N. Bumpus
Summary: Single-cell proteomics is a powerful tool for studying cellular heterogeneity and quantifying post-translational modifications. The authors developed a method with improved protein sequence coverage and demonstrated its application in characterizing the effects of inhibitor treatment in single human cells. This approach allows for the detection of multiple classes of post-translational modifications and provides insight into cell-to-cell variability in drug response that is missed by traditional proteomics.
NATURE COMMUNICATIONS
(2022)
Review
Physiology
Rongsong Li, Yang Xiao, Kang Li, Ling Tian
Summary: This review summarizes the recent research on autophagy regulated at both transcriptional and post-translational levels by insect hormone in cooperation with other signals, such as nutrient, in insects, providing a reference and deeper understanding for studying autophagy in insects.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemical Research Methods
Kyungbo Kim, Najla M. Albishi, Subba Reddy Palli
Summary: Recent studies have shown that JH-induced changes in the phosphorylation profiles of AaMet and AaKr-h1 contribute to their transcriptional activity. JH activation leads to alterations in the phosphorylation profiles of multiple residues of AaMet, while dephosphorylation of AaKr-h1 at phosphoserine-694 increases its transcriptional activity. These findings provide insights into how JH modulates key transcription factors and could aid in the development of novel insect control strategies.
JOURNAL OF PROTEOMICS
(2021)
Editorial Material
Cell Biology
Christian Agatemor, Sasa Ama Dyese Middleton, Daniela Toledo
Summary: Cells utilize post-translational and post-transcription modifications as crucial mechanisms to maintain homeostasis and regulate gene transcription. Recent discoveries have shown that these modifications are more pervasive and important than previously thought.
TRENDS IN CELL BIOLOGY
(2022)
Review
Cell Biology
Linbin Zhou, Danny Siu-Chun Ng, Jason C. Yam, Li Jia Chen, Clement C. Tham, Chi Pui Pang, Wai Kit Chu
Summary: This review summarizes recent findings on the impact of post-translational modifications (PTMs) on the functions of retinoblastoma protein (pRb). PTMs on pRb play crucial roles in cell cycle regulation, cell survival, and differentiation, and altered PTMs can lead to anomalies in cell proliferation and tumorigenesis. Understanding the post-translational modulation of pRb could facilitate the development of novel therapeutic strategies for various human diseases.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Review
Biotechnology & Applied Microbiology
Vida Rodriguez
Summary: The development of new strategies for the manufacture of peptide drug candidates, considering their increasing length and complexity, is becoming important. Enzymatic catalysis is gaining interest as a sustainable and efficient technology for peptide production. This review focuses on enzymes from ribosomally synthesized and post-translationally modified peptides biosynthesis pathways, discussing their ability to introduce stability-promoting structural features on peptides and their potential use in therapeutic peptide production processes.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Chemistry, Multidisciplinary
Julia Kraxner, Charlotta Lorenz, Julia Menzel, Iwan Parfentev, Ivan Silbern, Manuela Denz, Henning Urlaub, Blanche Schwappach, Sarah Koester
Summary: The mechanical properties of biological cells are influenced by the cytoskeleton, with phosphorylation serving as a fast mechanism for mechanical modulation. Phosphorylation can soften filaments, and the binding of 14-3-3 protein to phosphorylated filaments further enhances this effect, potentially preserving the softening and altering cell mechanics in the cell.
Review
Biochemistry & Molecular Biology
Wen Li, Feifei Li, Xia Zhang, Huikuan Lin, Chuan Xu
Summary: The occurrence and development of tumors depend on gene mutation and tumor heterogeneity, with the dynamic change of tumor microenvironment playing a crucial role. Protein post-translational modification (PTM) is important in regulating the tumor microenvironment.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Daniel Salas-Lloret, Roman Gonzalez-Prieto
Summary: Ubiquitination and SUMOylation are dynamic post-translational modifications that regulate cellular processes. The understanding of these modifications has been increased by the development of mass spectrometry-based approaches. This translated article provides a concise overview of the basic mechanisms of ubiquitination and SUMOylation, as well as recent MS-based approaches for specific target identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Lin Ling, Alexander S. Raikhel
Summary: Female mosquitoes feed on carbohydrates and proteins during each gonadotrophic cycle to become reproductively competent, with metabolism synchronized to support this process. The hormones juvenile hormone and ecdysteroids play key roles in female mosquito reproduction, while insulin-like peptides are critical for controlling metabolism. The JH and 20E pathways regulate protein levels of ILPs, establishing a regulatory axis for metabolism during gonadotrophic cycles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Oncology
Ana Gonzalez-Garcia, Antonio Garrido, Ana C. Carrera
Summary: Genetic alterations in the PI3-kinase/PTEN pathway are common in cancer cells. Current efforts to treat PTEN-dependent tumors mainly focus on PI3-kinase inhibition, but modulating PTEN post-translational modifications could provide alternative therapeutic strategies.
Article
Biochemistry & Molecular Biology
Hongwang Hu, Xuyu Yin, Suyun Pang, Yali Jiang, Qunfang Weng, Qiongbo Hu, Jingjing Wang
Summary: This study identified the most sensitive binding protein of Destruxin A (DA) as the juvenile hormone binding protein (JHBP) and investigated its role in regulating the physiological processes in silkworms. DA was found to inhibit body weight gain and accelerate pupation occurrence in silkworm larvae, while also modulating the content of free juvenile hormone (JH) in the hemolymph. Conformational changes were observed in both JHBP and the JHBP-JH binding complex upon interaction with DA. Molecular docking and point-mutant experiments suggested that DA may affect the N-arm of JHBP, which is responsible for JH binding. Additionally, JHBP was found to be widely distributed in various tissues of the silkworm. This study provides novel insights into the insecticidal mechanism of DA and enhances our understanding of the pathogenic process of Metarhizium.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ji Woo Park, Matthew D. Tyl, Ileana M. Cristea
Summary: The regulation of mitochondria structure and function is crucial for viral infections. Post-translational modification of mitochondrial proteins plays a critical role in controlling energy metabolism, apoptosis, and immune signaling. Understanding these modifications and their effects is important for the development of therapeutic strategies.
Review
Immunology
Jiaqi Kang, Jie Wu, Qinjie Liu, Xiuwen Wu, Yun Zhao, Jianan Ren
Summary: This review discusses the importance of STING in immune responses and its role in disease pathogenesis when over-activated. The focus is on post-translational modifications that regulate the activation and inhibition of STING, and their potential as targeted therapies.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Plant Sciences
Feng Yu, Ming Li, Dongli He, Pingfang Yang
Summary: Seed germination and seedling establishment are crucial developmental processes that undergo complex physiological changes, precisely regulated at transcriptional and translational levels. Phytohormones like ABA and GA play key roles in modulating this process, while proteins such as ABI5 and DELLA are central in seed germination. Post-translational modifications, particularly phosphorylation and ubiquitination, are shown to be decisive in regulating seed germination.
FRONTIERS IN PLANT SCIENCE
(2021)
Editorial Material
Entomology
Marek Jindra
Summary: This article discusses the development of insect growth regulators (IGRs) by focusing on insight into the molecular principles of biosynthetic or hormone signaling pathways unique to arthropods, with the aim of targeting specific insect taxa representing pests or disease vectors. Various strategies, such as rational structure-based design and high-throughput screening of chemical libraries, are used to obtain desired compounds for chemical intervention. All avenues leading to selective and environmentally safe pest control are valid in response to the declining world insect population.
JOURNAL OF PESTICIDE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Mehdi Sharifi Tabar, Caroline Giardina, Yue Feng, Habib Francis, Hakimeh Moghaddas Sani, Jason K. K. Low, Joel P. Mackay, Charles G. Bailey, John E. J. Rasko
Summary: This study provides insights into the protein-protein interaction network of the MGCC module and its role in gene regulation, particularly shedding light on the mechanisms underlying paralogue-specific functions and specificity.
Article
Biochemistry & Molecular Biology
Matej Milacek, Lenka Bittova, Sarka Tumova, Ondrej Luksan, Robert Hanus, Pavlina Kyjakova, Ales Machara, Ales Marek, Marek Jindra
Summary: Juvenile hormone controls insect reproduction and development through a receptor complex consisting of Met and Tai proteins, affecting vitellogenesis in cockroaches and caste polyphenism in termites. Studies show high-affinity binding of JH III by Met proteins in cockroaches and termites, with evidence of JH receptor agonist binding in hemimetabolous species.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Charlotte Hodson, Jason K. K. Low, Sylvie van Twest, Samuel E. Jones, Paolo Swuec, Vincent Murphy, Kaima Tsukada, Matthew Fawkes, Rohan Bythell-Douglas, Adelina Davies, Jessica K. Holien, Julienne J. O'Rourke, Benjamin L. Parker, Astrid Glaser, Michael W. Parker, Joel P. Mackay, Andrew N. Blackford, Alessandro Costa, Andrew J. Deans
Summary: The RecQ-like helicase BLM cooperates with topoisomerase IIIa to dissolve double Holliday junctions in homologous recombination. Mutations in the Bloom syndrome complex can cause genome instability and cancer susceptibility, including breast cancer.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Zhong Guo, Oleh Smutok, Wayne A. Johnston, Patricia Walden, Jacobus P. J. Ungerer, Thomas S. Peat, Janet Newman, Jake Parker, Tom Nebl, Caryn Hepburn, Artem Melman, Richard J. Suderman, Evgeny Katz, Kirill Alexandrov
Summary: The study aims to investigate the optimal strategy for developing artificial small molecule:protein complexes that function as chemically induced dimerization (CID) systems. By using methotrexate-controlled CID systems, the critical role of drug-induced conformational change in ligand-controlled protein complex assembly is revealed. The utility of the developed CID is demonstrated by constructing an electrochemical biosensor of methotrexate for quantification in human serum, as well as developing a multiplexed bioelectronic system for repeated measurements of multiple analytes using methotrexate and functionally related biosensor of rapamycin.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Claudio Counoupas, Matt D. Johansen, Alberto O. Stella, Duc H. Nguyen, Angela L. Ferguson, Anupriya Aggarwal, Nayan D. Bhattacharyya, Alice Grey, Owen Hutchings, Karishma Patel, Rezwan Siddiquee, Erica L. Stewart, Carl G. Feng, Nicole G. Hansbro, Umaimainthan Palendira, Megan C. Steain, Bernadette M. Saunders, Jason K. K. Low, Joel P. Mackay, Anthony D. Kelleher, Warwick J. Britton, Stuart G. Turville, Philip M. Hansbro, James A. Triccas
Summary: This study demonstrates the potential of a BCG-based vaccine to provide protection against major SARS-CoV-2 variants by inducing potent virus-specific immune responses. Pre-treatment with BCG:CoVac followed by a heterologous vaccine boosts SARS-CoV-2-specific antibody responses effectively neutralizing key variants.
Article
Biochemistry & Molecular Biology
Shreyas Arvindekar, Matthew J. Jackman, Jason K. K. Low, Michael J. Landsberg, Joel P. Mackay, Shruthi Viswanath
Summary: Using an integrative structure determination approach, researchers have revealed the molecular architecture of nucleosome deacetylase (NuDe) sub-complexes within the nucleosome remodeling and deacetylase (NuRD) complex. The study identified previously unrecognized subunit interfaces and located regions of unknown structure, resulting in the most complete and robustly cross-validated structural characterization of these NuRD sub-complexes to date.
Article
Biochemistry & Molecular Biology
Sarka Tumova, Marek Jindra
Summary: Juvenile hormone (JH) signalling plays a critical role in regulating insect development, but the molecular mechanisms are not well understood. This study investigated the dynamic aspect of JH signalling and revealed the rapid assembly and dissociation of JH receptor heterodimer from bHLH-PAS proteins upon JH binding. The study also demonstrated the regulatory role of the chaperone Hsp90/83 in the JH receptor complex formation.
Article
Multidisciplinary Sciences
Sarka Tumova, Matej Milacek, Ivan Snajdr, Magesh Muthu, Roman Tuma, David Reha, Pavel Jedlicka, Lenka Bittova, Adela Novotna, Pavel Majer, David Sedlak, Marek Jindra
Summary: This study reveals that peptidic juvenoids are highly potent and species-selective novel JHR agonists, which can inhibit insect metamorphosis and stimulate ovarian development in adult females.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Yichen Zhong, Hakimeh Moghaddas Sani, Bishnu P. Paudel, Jason K. K. Low, Ana P. G. Silva, Stefan Mueller, Chandrika Deshpande, Santosh Panjikar, Xavier J. Reid, Max J. Bedward, Antoine M. van Oijen, Joel P. Mackay
Summary: CHD4 is an essential ATP-dependent translocase that alters chromatin accessibility by repositioning histone octamers. The N-terminal intrinsically disordered region (IDR) promotes remodelling integrity, while the C-terminal region harbours an auto-inhibitory region that is relieved by binding to substrate DNA.
NATURE COMMUNICATIONS
(2022)
Article
Entomology
Marek Jindra
Summary: Frantisˇek Sehnal was a prominent figure in insect science, particularly in endocrinology, developmental biology, silk research, and insect interactions with genetically modified crops. This article provides an overview of Sehnal's research and other academic and educational activities, as well as personal experiences with him as a mentor. Despite his passing, Frantisˇek's legacy lives on.
JOURNAL OF INSECT PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Tara K. Bartolec, Xabier Vazquez-Campos, Alexander Norman, Clement Luong, Marcus Johnson, Richard J. Payne, Marc R. Wilkins, Joel P. Mackay, Jason K. K. Low
Summary: Significant advances in cryoelectron microscopy have expanded our ability to create structural models of proteins and protein complexes. Cross-linking mass spectrometry (XL-MS) is demonstrated to be a powerful tool for high-throughput experimental assessment of protein structures and interactions. The combination of XL-MS data with AlphaFold2 predictions offers opportunities to explore the structural proteome and interactome in depth.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Shikhar Sharma, Chi-Yeh Chung, Sean Uryu, Jelena Petrovic, Joan Cao, Amanda Rickard, Nataliya Nady, Samantha Greasley, Eric Johnson, Oleg Brodsky, Showkhin Khan, Hui Wang, Zhenxiong Wang, Yong Zhang, Konstantinos Tsaparikos, Lei Chen, Anthony Mazurek, John Lapek, Pei-Pei Kung, Scott Sutton, Paul F. Richardson, Eric C. Greenwald, Shinji Yamazaki, Rhys Jones, Karen A. Maegley, Patrick Bingham, Hieu Lam, Alexandra E. Stupple, Aileen Kamal, Anderly Chueh, Anthony Cuzzupe, Benjamin J. Morrow, Bin Ren, Catalina Carrasco-Pozo, Chin Wee Tan, Dharmesh D. Bhuva, Elizabeth Allan, Elliot Surgenor, Francois Vaillant, Havva Pehlivanoglu, Hendrik Falk, James R. Whittle, Janet Newman, Joseph Cursons, Judy P. Doherty, Karen L. White, Laura Macpherson, Mark Devlin, Matthew L. Dennis, Meghan K. Hattarki, Melanie De Silva, Michelle A. Camerino, Miriam S. Butler, Olan Dolezal, Patricia Pilling, Richard Foitzik, Paul A. Stupple, H. Rachel Lagiakos, Scott R. Walker, Soroor Hediyeh-Zadeh, Stewart Nuttall, Sukhdeep Spall, Susan A. Charman, Theresa Connor, Thomas S. Peat, Vicky M. Avery, Ylva E. Bozikis, Yuqing Yang, Ming Zhang, Brendon J. Monahan, Anne K. Voss, Ian P. Street, Sarah-Jane Dawson, Mark A. Dawson, Geoffrey J. Lindeman, Melissa J. Davis, Jane E. Visvader, Thomas A. Paul
Summary: In this study, a highly potent, selective, and orally bioavailable KAT6A/KAT6B inhibitor CTx-648 was identified, which inhibits H3K23 acetylation and exhibits anti-tumor activity in ER+ breast cancer. The mechanism of action may involve the modulation of transcriptional and epigenetic profiles.
CELL CHEMICAL BIOLOGY
(2023)