Article
Plant Sciences
Christopher J. Gisriel, Donald A. Bryant, Gary W. Brudvig, Tanai Cardona
Summary: This study investigates the molecular evolution of cyanobacterial adaptation mechanisms, specifically the Far-red light (FRL) photoacclimation (FaRLiP) mechanism. Phylogenetic analysis and structural data analysis suggest that FRL-specific photosystem I subunits arose relatively late in the evolution of cyanobacteria, and horizontal gene transfer may have contributed to the acquisition of FaRLiP in certain strains. The ancestral form of FRL-absorbing photosystem I is found to have specific structural characteristics.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Christopher J. Gisriel, Gaozhong Shen, David A. Flesher, Vasily Kurashov, John H. Golbeck, Gary W. Brudvig, Muhamed Amin, Donald A. Bryant
Summary: This study reports the structure of a dimeric FRL-PSII complex, revealing the absorption mechanism of far-red light and the formation of the complex. The interaction between Chls d and f and the protein is observed, and energy transfer is facilitated by the PsbH2 subunit and the PsbB2 bridge protein in the complex.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Christopher J. Gisriel, Eduard Elias, Gaozhong Shen, Nathan T. Soulier, David A. Flesher, M. R. Gunner, Gary W. Brudvig, Roberta Croce, Donald A. Bryant
Summary: To adapt to low-light environments, some cyanobacteria express a variant of the light-harvesting protein allophycocyanin (AP) that strongly absorbs far-red light (FRL). The structure-function relationship of this FRL-AP complex, expressed during acclimation to low light, was revealed using cryo-electron microscopy and time-resolved absorption spectroscopy. FRL-AP forms helical nanotubes instead of typical toroids due to altered domain geometry within each subunit. This study expands the understanding of light-harvesting proteins in nature and highlights the trade-off between resource accessibility and efficiency achieved through biological plasticity.
Article
Microbiology
Christopher J. Gisriel, Tanai Cardona, Donald A. Bryant, Gary W. Brudvig
Summary: This study reconstructs the ancestral sequence and performs structure-based molecular evolutionary studies on the far-red light (FRL)-specific subunits of FRL-PSII in cyanobacteria. The findings suggest that the duplications leading to the origin of specific subunits of FRL-PSII likely occurred prior to the diversification of extant cyanobacteria.
Article
Chemistry, Multidisciplinary
Jorge Follana-Berna, Rajaa Farran, Winfried Leibl, Annamaria Quaranta, Angela Sastre-Santos, Ally Aukauloo
Summary: Recent discovery challenges the textbook explanation of P-680 pigments as the red limit for oxygenic photosynthesis, as Chlf-absorbing at 727 nm in PSII is found to drive water oxidation. Synthesized Pc compounds mimic the cofactors of PSII, demonstrating potential for broader spectral window utilization in multi-electronic catalytic processes. Transient absorption studies reveal intramolecular electron transfer processes and formation of hydrogen-bonded phenoxyl radicals.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Craig MacGregor-Chatwin, Dennis J. Nurnberg, Philip J. Jackson, Cvetelin Vasilev, Andrew Hitchcock, Ming-Yang Ho, Gaozhong Shen, Christopher J. Gisriel, William H. J. Wood, Moontaha Mahbub, Vera M. Selinger, Matthew P. Johnson, Mark J. Dickman, Alfred William Rutherford, Donald A. Bryant, C. Neil Hunter
Summary: Cyanobacteria have developed strategies to adapt to different environments and utilize various strategies for photosynthesis. The study characterizes the molecular changes involved in FaRLiP and the supramolecular organization of photosystem I under FRL light, showing altered cellular distribution.
Article
Biochemistry & Molecular Biology
Christopher J. Gisriel, David A. Flesher, Gaozhong Shen, Jimin Wang, Ming-Yang Ho, Gary W. Brudvig, Donald A. Bryant
Summary: Some cyanobacteria can use far-red light for photosynthesis. The study identified the specific binding sites of chlorophyll f in photosystem I and revealed structural differences in far-red light-acclimated cyanobacteria.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biophysics
Anjue Mane Ara, Mohammad Kawsar Ahmed, Sandrine D'Haene, Henny van Roon, Cristian Ilioaia, Rienk van Grondelle, Md Wahadoszamen
Summary: This study conducted a Stark fluorescence spectroscopy analysis on photosystem II core antenna complexes CP43 and CP47 in both their native and aggregated states. The research revealed that aggregation-induced quenching in the core antenna complexes did not produce a far-red emission band, likely due to the absence of lutein1 in their structures. This suggests that the aggregation-induced conformational changes in the core antenna complexes may not lead to a chlorophyll-carotenoid interaction associated energy dissipation channel.
BIOPHYSICAL JOURNAL
(2021)
Article
Agronomy
Tingting Tan, Shenglan Li, Yuanfang Fan, Zhonglin Wang, Muhammad Ali Raza, Iram Shafiq, Beibei Wang, Xiaoling Wu, Taiwen Yong, Xiaochun Wang, Yushan Wu, Feng Yang, Wenyu Yang
Summary: This review discusses the impact of far-red light on plants, including the adjustment of photosynthetic electron transport and the path of light energy to improve plant photosynthetic capacity, as well as the regulation of leaf angle, plant height, and leaf area to capture more light energy.
Article
Plant Sciences
Shuyang Zhen, Marc W. van Iersel, Bruce Bugbee
Summary: The current definition of photosynthetically active radiation only considers photons from 400 to 700 nm, disregarding the synergistic interaction between far-red photons and shorter-wavelength photons. In our study, we used a filter to assess the effects of far-red photons on photosynthesis in various species under different light conditions. We found that far-red photons play an important role in photosynthesis, especially in vegetation shade. Accounting for the photosynthetic activity of far-red photons is crucial for accurate measurement and modeling of photosynthesis at different scales.
Article
Biochemistry & Molecular Biology
Eduard Elias, Nicoletta Liguori, Yoshitaka Saga, Judith Schafers, Roberta Croce
Summary: The study demonstrates that enhancing the far-red absorption of plants by binding Chl d to LHCII complex can effectively increase the absorption cross section without altering the functional architecture. The reconstituted complex maintains native LHC architecture and exhibits functional capabilities in light harvesting and excitation energy transfer. Overall, it is possible to obtain plant LHCs with enhanced far-red absorption and intact functional properties through this strategy.
Article
Cell Biology
Krzysztof M. Tokarz, Wojciech Makowski, Barbara Tokarz, Ewa Muszynska, Zbigniew Gajewski, Stanislaw Mazur, Edward Kunicki, Olgierd Jeremiasz, Piotr Sobik, Pawel Nowak, Karolina Miernicka, Kinga Mrzyglod, Piotr Rozpadek
Summary: The study aimed to investigate the impact of modified light spectrum on the photosynthetic performance of lettuce. The use of glass with red luminophore altered the sunlight spectrum, resulting in changes in the efficiency parameters, chloroplast ultrastructure, and structural proteins of the photosynthetic apparatus. These changes led to a decrease in the CO2 carboxylation efficiency of lettuce.
Article
Biochemistry & Molecular Biology
Fenghua Wu, Xiuxiu Li, Gongxian Yang, Jince Song, Xiaoyu Zhao, Lixia Zhu, Xiaochun Qin
Summary: In this study, LHCA5 overexpression plants were constructed in Arabidopsis and the effects of LHCA5 substituting for LHCA4 within the PSI-LHCI super-complex on photosynthetic properties were investigated. The results showed that LHCA5 can replace LHCA4 and lead to a blue shift in low temperature fluorescence emission.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biology
Orkun Coruh, Anna Frank, Hideaki Tanaka, Akihiro Kawamoto, Eithar El-Mohsnawy, Takayuki Kato, Keiichi Namba, Christoph Gerle, Marc M. Nowaczyk, Genji Kurisu
Summary: A study reports the structure of monomeric Photosystem I from cyanobacteria Thermosynechoccocus elongatus BP-1, revealing insights into monomerization-induced changes in both the central trimerization domain and the peripheral regions of the complex, as well as linking monomerization-induced loss of red chlorophylls to a cluster of chlorophylls adjacent to PsaX. The findings propose a role of PsaX in stabilizing red chlorophylls and suggest that lipids in the surrounding membrane play a major role in uphill excitation energy transfer from red chlorophylls to P700.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemical Research Methods
Ting-So Liu, Ke-Feng Wu, Han-Wei Jiang, Kai-Wen Chen, Ting-Shuo Nien, Donald A. Bryant, Ming-Yang Ho
Summary: With the increasing demand for sustainable energy, photoautotrophic cyanobacteria have become a popular platform for synthetic biology tools. However, genetic tools have not yet been developed for many cyanobacteria strains suitable for industrial applications. This study established a conjugation-based method for gene expression in Chlorogloeopsis fritschii PCC 9212 and identified a promoter activated by far-red light, providing an additional option for regulating gene expression in cyanobacteria.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Plant Sciences
Gregory S. Orf, Christopher J. Gisriel, Jesse Granstrom, Patricia L. Baker, Kevin E. Redding
Summary: The study reveals that PshX in Heliobacterium modesticaldum acts as a low-energy antenna subunit involved in energy transfer process. The research also demonstrates the importance of PshX in the stability and evolution of HbRC.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Christopher J. Gisriel, David A. Flesher, Gaozhong Shen, Jimin Wang, Ming-Yang Ho, Gary W. Brudvig, Donald A. Bryant
Summary: Some cyanobacteria can use far-red light for photosynthesis. The study identified the specific binding sites of chlorophyll f in photosystem I and revealed structural differences in far-red light-acclimated cyanobacteria.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Christopher J. Gisriel, Jimin Wang, Jinchan Liu, David A. Flesher, Krystle M. Reiss, Hao-Li Huang, Ke R. Yang, William H. Armstrong, M. R. Gunner, Victor S. Batista, Richard J. Debus, Gary W. Brudvig
Summary: The cryo-electron microscopy structure of PSII from Synechocystis sp. PCC 6803 has been determined at high resolution, revealing differences from thermophilic cyanobacterial PSII structures that significantly impact the understanding of PSII structure and the mechanism of water oxidation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
David A. Flesher, Jinchan Liu, Jessica M. Wiwczar, Krystle Reiss, Ke R. Yang, Jimin Wang, Mikhail Askerka, Christopher J. Gisriel, Victor S. Batista, Gary W. Brudvig
Summary: This study explores the effect of glycerol on the relative stability of different intermediate states of the oxygen-evolving complex in photosystem II. The results show that glycerol disrupts the hydrogen-bond network in the narrow channel, leading to an increase in the stability of a specific high-spin state.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Plant Sciences
Nathan Soulier, Karim Walters, Tatiana N. Laremore, Gaozhong Shen, John H. Golbeck, Donald A. Bryant
Summary: Based on their growth responses to high and low irradiance, thermophilic Synechococcus sp. isolates from Mushroom Spring in Yellowstone National Park can be classified as high-light or low-light ecotypes. The low-light ecotypes show increased growth at low irradiance and develop far-red absorbance and fluorescence after growth in low light. These ecotypes have unique gene clusters encoding cyanobacteriochrome, sensor histidine kinase, far-red light absorbing allophycocyanin, and a chlorophyll a-binding protein. The emergence of far-red absorbance in low-light adapted cells is a result of a novel antenna complex containing far-red light absorbing allophycocyanin. Additionally, the two GAF domains of the cyanobacteriochrome LcyA bind different ligands that potentially enable this photoreceptor to respond to various environmental factors.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Microbiology
Bayleigh Murray, Emine Ertekin, Micah Dailey, Nathan T. Soulier, Gaozhong Shen, Donald A. Bryant, Cesar Perez-Fernandez, Jocelyne DiRuggiero
Summary: In hyper-arid deserts, endolithic cyanobacteria adapt to the far-red light environment by synthesizing chlorophyll f. Through Far-Red-Light Photoacclimation (FaRLiP) response, the cyanobacteria are able to adjust their photosynthetic apparatus and modify their light-harvesting complexes.
Article
Biochemistry & Molecular Biology
Christopher J. Gisriel, Gaozhong Shen, David A. Flesher, Vasily Kurashov, John H. Golbeck, Gary W. Brudvig, Muhamed Amin, Donald A. Bryant
Summary: This study reports the structure of a dimeric FRL-PSII complex, revealing the absorption mechanism of far-red light and the formation of the complex. The interaction between Chls d and f and the protein is observed, and energy transfer is facilitated by the PsbH2 subunit and the PsbB2 bridge protein in the complex.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jimin Wang, Jinchan Liu, Christopher J. Gisriel, Shenping Wu, Federica Maschietto, David A. Flesher, Elias Lolis, George P. Lisi, Gary W. Brudvig, Yong Xiong, Victor S. Batista
Summary: This article describes a method for correcting relative voxel scaling factors in cryo-EM maps, which reduces amplitude differences and reveals structural differences. Additionally, this method uncovers previously overlooked differences and reveals local structural changes.
JOURNAL OF STRUCTURAL BIOLOGY
(2022)
Article
Plant Sciences
David J. Vinyard
Summary: Unlike laboratory conditions, real environments have dynamic light intensity. Researchers have developed a simple and affordable system that uses a commercial dimmable LED panel to simulate a sinusoidal function representing daylight hours and includes stochastic shading events. The system closely replicates light intensity measurements on Earth's surface during partly cloudy days or in lower levels of plant canopies. This tool may be valuable for researchers studying photosynthetic acclimation responses.
PHOTOSYNTHESIS RESEARCH
(2023)
Article
Biochemical Research Methods
Ting-So Liu, Ke-Feng Wu, Han-Wei Jiang, Kai-Wen Chen, Ting-Shuo Nien, Donald A. Bryant, Ming-Yang Ho
Summary: With the increasing demand for sustainable energy, photoautotrophic cyanobacteria have become a popular platform for synthetic biology tools. However, genetic tools have not yet been developed for many cyanobacteria strains suitable for industrial applications. This study established a conjugation-based method for gene expression in Chlorogloeopsis fritschii PCC 9212 and identified a promoter activated by far-red light, providing an additional option for regulating gene expression in cyanobacteria.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Chemistry, Physical
Divya Kaur, Bryan Ferlez, Patrick Landry, Till Biskup, Stefan Weber, John H. Golbeck, K. V. Lakshmi, Art van der Est
Summary: This study confirms the functionality of the partially converted BChl g' / Chl a(F)' special pair in samples exposed to dioxygen. The energy of the charge-separated state increases significantly when both BChl g' molecules of the P-800 dimer are converted.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Multidisciplinary Sciences
Craig MacGregor-Chatwin, Dennis J. Nurnberg, Philip J. Jackson, Cvetelin Vasilev, Andrew Hitchcock, Ming-Yang Ho, Gaozhong Shen, Christopher J. Gisriel, William H. J. Wood, Moontaha Mahbub, Vera M. Selinger, Matthew P. Johnson, Mark J. Dickman, Alfred William Rutherford, Donald A. Bryant, C. Neil Hunter
Summary: Cyanobacteria have developed strategies to adapt to different environments and utilize various strategies for photosynthesis. The study characterizes the molecular changes involved in FaRLiP and the supramolecular organization of photosystem I under FRL light, showing altered cellular distribution.