Review
Microbiology
Richard Galajda, Alexandra Valencakova, Monika Sucik, Petra Kandracova
Summary: Nosematosis is a honey bee disease caused by Nosema apis and Nosema ceranae, with exposure to pesticides and nutritional stress being key factors that weaken the immune response of bees and make them more susceptible to parasites. Polymerase chain reaction (PCR) is an important method for detecting and distinguishing between the two species causing the disease.
Article
Veterinary Sciences
Sigmar Naudi, Juris Steiselis, Margret Jurison, Risto Raimets, Lea Tummeleht, Kristi Praakle, Arvi Raie, Reet Karise
Summary: The study aimed to investigate the prevalence of Nosema spp. in Estonia and Latvia, as well as the persistence of the disease in previously infected hives.
VETERINARY SCIENCES
(2021)
Article
Microbiology
Carolina Bartolome, Clara Jabal-Uriel, Maria Buendia-Abad, Maria Benito, Concepcion Ornosa, Pilar De la Rua, Raquel Martin-Hernandez, Mariano Higes, Xulio Maside
Summary: Assessing parasite diversity in bumblebees requires advanced molecular tools, and next-generation sequencing technology proved to be effective in detecting a higher diversity of parasite species in individual specimens. The study revealed new parasite taxa not previously reported in these hosts, highlighting the importance of using multiple markers to minimize oversight of target organisms. The deep sequencing methodology provided a more comprehensive way to explore parasite diversity and offered new insights that need further analysis for the health of bumblebees.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Entomology
Emma L. Bradford, Casey L. Gregory, Arturo Roman Longoria, Korin Rex Jones, Emma K. Bueren, David C. Haak, Richard Fell, Lisa K. Belden
Summary: Nosema ceranae and Nosema apis are microsporidian parasites that cause disease in honey bees. Developing new and improved methods for detecting and quantifying infection is crucial. The researchers designed a new duplex qPCR assay that accurately quantifies both N. ceranae and N. apis infection. The assay demonstrated good linearity and correlation with microscopic spore counts. The researchers also investigated Nosema infection in hive surveys and low dose experimental exposure, revealing variations in infection levels.
JOURNAL OF APICULTURAL RESEARCH
(2022)
Article
Entomology
Anastasia N. Ignatieva, Sergey A. Timofeev, Yuri S. Tokarev, Viacheslav V. Dolgikh
Summary: This study presents a simple and efficient method for cultivating V. ceranae using artificially infected worker bees in the laboratory, providing a feasible solution for mass replication of this pathogen.
Article
Entomology
Erkay Ozgor
Summary: The study reveals that greater wax moths exhibit resistance towards Nosema species, while honeybees have some mortality rates in single and mixed infection cases. The level of phenoloxidase gene expression remains stable in the wax moth, while it increases in honeybees under infection.
Article
Veterinary Sciences
Pablo Jesus Marin-Garcia, Yoorana Peyre, Ana Elena Ahuir-Baraja, Maria Magdalena Garijo, Lola Llobat
Summary: Honeybee populations have declined due to both biotic and abiotic factors, with infection by the microsporidia Nosema ceranae being a significant cause. The infection can lead to important damage in bees, resulting in decreased numbers and colony loss. Alternative treatments such as natural products and probiotics are being studied. Prophylaxis and management of affected colonies are crucial for disease control, while considering other factors such as the use of insecticides.
VETERINARY SCIENCES
(2022)
Article
Entomology
Poonnawat Panjad, Rujipas Yongsawas, Chainarong Sinpoo, Chonthicha Pakwan, Phakamas Subta, Sasiprapa Krongdang, Ammarin In-on, Siriwadee Chomdej, Panuwan Chantawannakul, Terd Disayathanoowat
Summary: The study revealed that Nosema ceranae impacted gut bacteria in adult honeybees, while Paenibacillus larvae did not affect gut bacteria in larvae.
Article
Environmental Sciences
Alvaro Uruena, Nuria Blasco-Lavilla, Pilar De la Rua
Summary: Honey bees' health is affected by various factors, including agrochemical use and diseases. This study examines the interaction between pesticides and Nosema ceranae, a microsporidium, and their effects on gene expression, sugar consumption, and mortality in honey bees. The results show that exposure to sulfoxaflor increases sugar intake and alters gene expression in bees infected with N. ceranae. Additionally, sulfoxaflor is the only pesticide that increased honey bee mortality. These findings highlight the importance of studying the interaction between stressors to understand their impact on honey bee and wild bee health.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Review
Microbiology
Magdalena Kunat-Budzynska, Michal Budzynski, Michal Schulz, Aneta Strachecka, Marek Gancarz, Robert Rusinek, Aneta A. Ptaszynska
Summary: This publication provides an overview of the impact of nosemosis on honeybees, as well as research on natural and synthetic compounds for the treatment and prevention of this disease. With the withdrawal of antibiotics like fumagillin, researchers are searching for alternative substances that can effectively combat nosemosis without harming bees.
Article
Microbiology
Dae Yoon Kim, Soohyun Maeng, Sung-Jin Cho, Hui Jin Park, Kyungsu Kim, Jae Kwon Lee, Sathiyaraj Srinivasan
Summary: The declining honeybee populations pose a significant risk to global agriculture, and parasites are a major contributing factor. In recent years, there has been increasing attention to honeybee diseases, with annual losses of 30% to 40% of managed colonies in the USA. This study aims to compare the bacterial community in the gut of honeybees infected with Nosema ceranae and Ascosphaera apis, and compare it to that of weakly active honeybees.
Article
Veterinary Sciences
Eduardo Jose Garcia-Vicente, Maria Martin, Ismael Rey-Casero, Ana Perez, Remigio Martinez, Maria Bravo, Juan Manuel Alonso, David Risco
Summary: Currently, beekeeping is facing risks due to the deteriorating health of honeybees, leading to high mortality rates, especially in winter. The emergence/re-emergence of communicable diseases is a significant consequence, endangering the industry's continuity without effective treatments and harmful residues on wax or honey. This study aimed to evaluate the impact of feed supplementation with probiotic and postbiotic products on the strength, population, and sanitary parameters of honey bees. The results showed that hives consuming postbiotic products exhibited improved strength, increased bee population and egg laying, and maintained pollen reserves, suggesting that postbiotics could be a valuable tool for beekeepers to enhance hive health.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Ecology
Cristina Botias, Julia C. Jones, Tobias Pamminger, Ignasi Bartomeus, William O. H. Hughes, Dave Goulson
Summary: Bumblebees exposed to combinations of stressors, including pesticides and pathogens, exhibit detrimental effects on individual and colony health, leading to reduced food collection, colony growth, and brood production. Understanding the interactions between different stressors is crucial for managing bee populations and maintaining pollination services in the future.
JOURNAL OF ANIMAL ECOLOGY
(2021)
Article
Entomology
Dong-Jun Kim, Ra-Mi Woo, Kyu-Seek Kim, Soo-Dong Woo
Summary: This study evaluated the possibility of using entomopathogenic fungal culture extracts with various biological activities to control honeybee nosemosis. Many of the tested extracts showed high inhibitory activity on Nosema ceranae spores. It was confirmed for the first time that the fungal culture extract could not only suppress honeybee nosemosis but also increase their lifespan. The extracts from two fungal isolates, Paecilomyces marquandii 364 and Pochonia bulbillosa 60, showed the most promising results in reducing honeybee mortality and increasing their survival.
Article
Entomology
Yi-Hsuan Li, Zih-Ting Chang, Ming-Ren Yen, Yu-Feng Huang, Tzu-Han Chen, Ju-Chun Chang, Ming-Cheng Wu, Yu-Liang Yang, Yue-Wen Chen, Yu-Shin Nai
Summary: This study investigated the gene expression profile of Nosema ceranae, a fungal parasite of honey bees, at different time points post-infection. The results revealed that the gene expression of N. ceranae during infection is closely related to gene transcription, protein synthesis, and structural proteins. The findings have important implications for the control of nosemosis in apiculture.
Article
Agronomy
Viola Kurm, Ilse Houwers, Claudia E. Coipan, Peter Bonants, Cees Waalwijk, Theo Van der Lee, Balazs Brankovics, Jan Van der Wolf
Summary: Identification and classification of members of the Ralstonia solanacearum species complex (RSSC) is challenging due to the heterogeneity of this complex. Whole genome sequence data of 225 strains were used to classify strains based on average nucleotide identity (ANI) and multilocus sequence analysis (MLSA). Results showed that most RSSC strains could be grouped into different species and phylotypes based on ANI score, with differences found at SNP sites in different subgroups. Computational analysis of TaqMan and PCR assays revealed potential false positives or negatives, suggesting the need for careful selection of assays for target organisms within the RSSC.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Plant Sciences
David M. Geiser, Abdullah M. S. Al-Hatmi, Takayuki Aoki, Tsutomu Arie, Virgilio Balmas, Irene Barnes, Gary C. Bergstrom, Madan K. Bhattacharyya, Cheryl L. Blomquist, Robert L. Bowden, Balazs Brankovics, Daren W. Brown, Lester W. Burgess, Kathryn Bushley, Mark Busman, Jose F. Cano-Lira, Joseph D. Carrillo, Hao-Xun Chang, Chi-Yu Chen, Wanquan Chen, Martin Chilvers, Sofia Chulze, Jeffrey J. Coleman, Christina A. Cuomo, Z. Wilhelm de Beer, G. Sybren de Hoog, Johanna Del Castillo-Munera, Emerson M. Del Ponte, Javier Dieguez-Uribeondo, Antonio Di Pietro, Veronique Edel-Hermann, Wade H. Elmer, Lynn Epstein, Akif Eskalen, Maria Carmela Esposto, Kathryne L. Everts, Sylvia P. Fernandez-Pavia, Gilvan Ferreira da Silva, Nora A. Foroud, Gerda Fourie, Rasmus J. N. Frandsen, Stanley Freeman, Michael Freitag, Omer Frenkel, Kevin K. Fuller, Tatiana Gagkaeva, Donald M. Gardiner, Anthony E. Glenn, Scott E. Gold, Thomas R. Gordon, Nancy F. Gregory, Marieka Gryzenhout, Josep Guarro, Beth K. Gugino, Santiago Gutierrez, Kim E. Hammond-Kosack, Linda J. Harris, Monika Homa, Cheng-Fang Hong, Laszlo Hornok, Jenn-Wen Huang, Macit Ilkit, Adriaana Jacobs, Karin Jacobs, Cong Jiang, Maria Del Mar Jimenez-Gasco, Seogchan Kang, Matthew T. Kasson, Kemal Kazan, John C. Kennell, Hye-Seon Kim, H. Corby Kistler, Gretchen A. Kuldau, Tomasz Kulik, Oliver Kurzai, Imane Laraba, Matthew H. Laurence, Theresa Lee, Yin-Won Lee, Yong-Hwan Lee, John F. Leslie, Edward C. Y. Liew, Lily W. Lofton, Antonio F. Logrieco, Manuel S. Lopez-Berges, Alicia G. Luque, Erik Lysoe, Li-Jun Ma, Robert E. Marra, Frank N. Martin, Sara R. May, Susan P. McCormick, Chyanna McGee, Jacques F. Meis, Quirico Migheli, N. M. I. Mohamed Nor, Michel Monod, Antonio Moretti, Diane Mostert, Giuseppina Mule, Francoise Munaut, Gary P. Munkvold, Paul Nicholson, Marcio Nucci, Kerry O'Donnell, Matias Pasquali, Ludwig H. Pfenning, Anna Prigitano, Robert H. Proctor, Stephane Ranque, Stephen A. Rehner, Martijn Rep, Gerardo Rodriguez-Alvarado, Lindy Joy Rose, Mitchell G. Roth, Carmen Ruiz-Roldan, Amgad A. Saleh, Baharuddin Salleh, Hyunkyu Sang, Maria Mercedes Scandiani, Jonathan Scauflaire, David G. Schmale, Dylan P. G. Short, Adnan Sisic, Jason A. Smith, Christopher W. Smyth, Hokyoung Son, Ellie Spahr, Jason E. Stajich, Emma Steenkamp, Christian Steinberg, Rajagopal Subramaniam, Haruhisa Suga, Brett A. Summerell, Antonella Susca, Cassandra L. Swett, Christopher Toomajian, Terry J. Torres-Cruz, Anna M. Tortorano, Martin Urban, Lisa J. Vaillancourt, Gary E. Vallad, Theo A. J. van der Lee, Dan Vanderpool, Anne D. van Diepeningen, Martha M. Vaughan, Eduard Venter, Marcele Vermeulen, Paul E. Verweij, Altus Viljoen, Cees Waalwijk, Emma C. Wallace, Grit Walther, Jie Wang, Todd J. Ward, Brian L. Wickes, Nathan P. Wiederhold, Michael J. Wingfield, Ana K. M. Wood, Jin-Rong Xu, Xiao-Bing Yang, Tapani Yli-Mattila, Sung-Hwan Yun, Latiffah Zakaria, Hao Zhang, Ning Zhang, Sean X. Zhang, Xue Zhang
Summary: The debate over the classification of the Fusarium genus in recent years has focused on whether the FSSC should be included in Fusarium, but molecular phylogenetic research has shown strong support for the monophyly of Fusarium including the FSSC, which is considered the best taxonomic option available.
Article
Plant Sciences
Jiang Tan, Noemie De Zutter, Sarah De Saeger, Marthe De Boevre, Trang Minh Tran, Theo van der Lee, Cees Waalwijk, Anne Willems, Peter Vandamme, Maarten Ameye, Kris Audenaert
Summary: The study investigated the efficacy of chemical and biocontrol agents against F. graminearum in wheat ears, showing that both fungicide and actinobacterial strains were able to reduce FHB symptoms and mycotoxin production. Co-inoculation with F. poae significantly reduced the efficacy of control strategies.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Eef M. Jonkheer, Balazs Brankovics, Ilse M. Houwers, Jan M. van der Wolf, Peter J. M. Bonants, Robert A. M. Vreeburg, Robert Bollema, Jorn R. de Haan, Lidija Berke, Sandra Smit, Dick de Ridder, Theo A. J. van der Lee
Summary: This study utilized a pangenomic approach to analyze the genetic diversity of Pectobacterium genus, revealing the composition and genetic variations at the genomic level. Results showed that Pectobacterium genus has a high proportion of core genes, as well as specific genes associated with virulence. The research indicates that evolution in Pectobacteria involves dynamic processes such as gene acquisitions, genome rearrangements, and gene losses.
Article
Entomology
Marga Van Gent-Pelzer, Bram Cornelissen
Summary: A novel approach of employing swab sample collection of frass for real-time PCR detection of the small hive beetle (SHB) has been successfully tested in this study, showing potential for improving diagnostics and early detection of SHB.
JOURNAL OF APICULTURAL RESEARCH
(2021)
Review
Plant Sciences
Emerson M. Del Ponte, Glaucia M. Moreira, Todd J. Ward, Kerry O'Donnell, Camila P. Nicolli, Franklin J. Machado, Maira R. Duffeck, Kaique S. Alves, Dauri J. Tessmann, Cees Waalwijk, Theo van der Lee, Hao Zhang, Sofia N. Chulze, Sebastian A. Stenglein, Dinorah Pan, Silvana Vero, Lisa J. Vaillancourt, David G. Schmale, Paul D. Esker, Antonio Moretti, Antonio F. Logrieco, H. Corby Kistler, Gary C. Bergstrom, Altus Viljoen, Lindy J. Rose, Gert J. van Coller, Theresa Lee
Summary: Fusarium graminearum, one of the top five destructive fungal pathogens affecting agroecosystems, was systematically reviewed in this study. Two main data tables were created, summarizing information on species and chemotypes of 16,274 strains sampled across six continents and 32 countries. The publicly available database and interactive interface allow for searches, summarization, and mapping of strain distribution. The findings provide valuable guidance for future surveys and investigations into factors related to species distribution, such as climate and land use. Authors are encouraged to contribute data at the strain level to the accessible database.
Article
Plant Sciences
Bart. T. L. H. van de Vossenberg, Charlotte Prodhomme, Jack H. Vossen, Theo A. J. van der Lee
Summary: This review provides an overview of the importance of potato wart disease and the research progress on the pathogen and its host. It covers taxonomy, epidemiology, pathology, and pathogen-host interaction.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Zhen Yan, Wanquan Chen, Theo van der Lee, Cees Waalwijk, Anne D. van Diepeningen, Jie Feng, Hao Zhang, Taiguo Liu
Summary: Exploiting wheat cultivars with stable resistance to Fusarium Head blight and toxin accumulation is an effective strategy to reduce yield losses and mycotoxin contamination. Investigation in China's major wheat growing regions revealed that growing region and cultivar significantly affect the occurrence of FHB and mycotoxin accumulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Eef M. Jonkheer, Dirk-Jan M. van Workum, Siavash Sheikhizadeh Anari, Balazs Brankovics, Jorn R. de Haan, Lidija Berke, Theo A. J. van der Lee, Dick de Ridder, Sandra Smit
Summary: This article presents the latest update of PanTools, which enables the construction and comparative analysis of pangenomes, as well as the integration of functional annotations and phylogenetic analysis.
Article
Multidisciplinary Sciences
Wasin Poncheewin, Anne D. van Diepeningen, Theo A. J. van der Lee, Maria Suarez-Diez, Peter J. Schaap
Summary: This study used genome properties and machine learning to establish the relationship between Pseudomonas strains isolated from the rhizosphere and phyllosphere and their plant-associated lifestyle. It identified 28 discriminating features and demonstrated the potential of genome properties annotation as a computational tool for classifying plant-associated lifestyles.
SCIENTIFIC REPORTS
(2022)
Article
Agronomy
Inge M. Krijger, Max Strating, Marga van Gent-Pelzer, Theo A. J. van der Lee, Sara A. Burt, Fleur H. Schroeten, Robin de Vries, Marieke de Cock, Miriam Maas, Bastiaan G. Meerburg
Summary: Rodents in the Netherlands have developed resistance to anticoagulant rodenticides, particularly brodifacoum. A study found genetic mutations at codon 139 of the Vkorc1 gene in both Norway rats and house mice. These results provide a baseline for integrated pest management strategies in the Netherlands.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Ping Zhang, Hao Xin, Theo van der Lee
Summary: This study integrates transcriptome and metabolome sequencing data to investigate the impact of Armillaria solidipes on Bacillus velezensis BY6. The growth of BY6 is inhibited by A. solidipes, causing damage to the cell wall and membrane. Gene and metabolite network analysis reveals the involvement of several pathways in the inhibition of BY6's biocontrol ability. This knowledge contributes to a better understanding of the mechanism and potential targets for overcoming drug resistance.
BIOLOGICAL CONTROL
(2023)
Article
Forestry
Ping Zhang, Mingru Kong, Guangqiang Xie, Theo van der Lee, Lihai Wang, Yanqiu Xing
Summary: The article introduces a method for monitoring root infection using X-ray computed tomography, and verifies and measures parameters in the interaction between poplar and Armillaria solidipes.