Significantly Improving the Thermostability and Catalytic Efficiency of Streptomyces mobaraenesis Transglutaminase through Combined Rational Design

Effect of introducing a disulfide bridge on the thermostability of microbial transglutaminase from Streptomyces mobaraensis

(2021) Keiichi Yokoyama et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Surface charge engineering of Thermomyces lanuginosus lipase improves enzymatic activity and biodiesel synthesis

(2021) Nanyu Han et al.   BIOTECHNOLOGY LETTERS

Engineering diacetylchitobiose deacetylase from Pyrococcus horikoshii towards an efficient glucosamine production

(2021) Ziyang Huang et al.   BIORESOURCE TECHNOLOGY

Properties of transglutaminase‐induced myofibrillar/wheat gluten gels

(2021) Yuanyuan Ouyang et al.   JOURNAL OF FOOD SCIENCE

Design of a PL18 alginate lyase with flexible loops and broader entrance to enhance the activity and thermostability

(2021) Bingmei Su et al.   ENZYME AND MICROBIAL TECHNOLOGY

Surface charge-based rational design of aspartase modifies the optimal pH for efficient β-aminobutyric acid production

(2020) Yaling Wang et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Enzymatic activity and thermoresistance of improved microbial transglutaminase variants

(2019) B. Böhme et al.   AMINO ACIDS

Significantly improved catalytic efficiency of caffeic acid O-methyltransferase towards N-acetylserotonin by strengthening its interactions with the unnatural substrate’s terminal structure

(2019) Wenya Wang et al.   ENZYME AND MICROBIAL TECHNOLOGY

Predicting changes in protein stability caused by mutation using sequence- and structure-based methods in a CAGI5 blind challenge

(2019) Alexey Strokach et al.   HUMAN MUTATION

More bang for your buck: Improved use of GPU nodes for GROMACS 2018

(2019) Carsten Kutzner et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Semi-rational design and molecular dynamics simulations study of the thermostability enhancement of cellobiose 2-epimerases

(2019) Qiuming Chen et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design

(2018) Caterina Martin et al.   Biotechnology for Biofuels

Analysis and Evaluation of the Inhibitory Mechanism of a Novel Angiotensin-I-Converting Enzyme Inhibitory Peptide Derived from Casein Hydrolysate

(2018) Maolin Tu et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

FoldX as Protein Engineering Tool: Better Than Random Based Approaches?

(2018) Oliver Buß et al.   Computational and Structural Biotechnology Journal

Disulfide bonds elimination of endoglucanase II from Trichoderma reesei by site-directed mutagenesis to improve enzyme activity and thermal stability: An experimental and theoretical approach

(2018) Ali Akbarzadeh et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Structure of a glutamine donor mimicking inhibitory peptide shaped by the catalytic cleft of microbial transglutaminase

(2018) Norbert E. Juettner et al.   FEBS Journal

Coupled molecular dynamics mediate long- and short-range epistasis between mutations that affect stability and aggregation kinetics

(2018) Haoran Yu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Site-Specific Transglutaminase-Mediated Conjugation of Interferon α-2b at Glutamine or Lysine Residues

(2016) Barbara Spolaore et al.   BIOCONJUGATE CHEMISTRY

One-pot peptide and protein conjugation: a combination of enzymatic transamidation and click chemistry

(2016) N. M. Rachel et al.   CHEMICAL COMMUNICATIONS

Simultaneous Optimization of Biomolecular Energy Functions on Features from Small Molecules and Macromolecules

(2016) Hahnbeom Park et al.   Journal of Chemical Theory and Computation

Protein engineering of Bacillus acidopullulyticus pullulanase for enhanced thermostability using in silico data driven rational design methods

(2015) Ana Chen et al.   ENZYME AND MICROBIAL TECHNOLOGY

ff14SB: Improving the Accuracy of Protein Side Chain and Backbone Parameters from ff99SB

(2015) James A. Maier et al.   Journal of Chemical Theory and Computation

The I-TASSER Suite: protein structure and function prediction

(2015) Jianyi Yang et al.   NATURE METHODS

I-TASSER server: new development for protein structure and function predictions

(2015) Jianyi Yang et al.   NUCLEIC ACIDS RESEARCH

Transglutaminase-Based Chemo-Enzymatic Conjugation Approach Yields Homogeneous Antibody–Drug Conjugates

(2014) Patrick Dennler et al.   BIOCONJUGATE CHEMISTRY

Versatility of Microbial Transglutaminase

(2014) Pavel Strop   BIOCONJUGATE CHEMISTRY

Enhanced Enzyme Kinetic Stability by Increasing Rigidity within the Active Site

(2014) Yuan Xie et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Transglutaminase and high pressure effects on heat-induced gelation of Alaska pollock (Theragra chalcogramma) surimi

(2014) Zhiwei Zhu et al.   JOURNAL OF FOOD ENGINEERING

A novel approach for improving the yield of Bacillus subtilis transglutaminase in heterologous strains

(2014) Yihan Liu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Alternative Computational Protocols for Supercharging Protein Surfaces for Reversible Unfolding and Retention of Stability

(2013) Bryan S. Der et al.   PLoS One

Structure-Based Design of Supercharged, Highly Thermoresistant Antibodies

(2012) Aleksandr E. Miklos et al.   CHEMISTRY & BIOLOGY

An overview of the Amber biomolecular simulation package

(2012) Romelia Salomon-Ferrer et al.   Wiley Interdisciplinary Reviews-Computational Molecular Science

Investigations on the activation of recombinant microbial pro-transglutaminase: in contrast to proteinase K, dispase removes the histidine-tag

(2011) Christian Sommer et al.   AMINO ACIDS

Increased thermostability of microbial transglutaminase by combination of several hot spots evolved by random and saturation mutagenesis

(2011) Karin Buettner et al.   AMINO ACIDS

Screening for improved activity of a transglutaminase from Streptomyces mobaraensis created by a novel rational mutagenesis and random mutagenesis

(2010) Keiichi Yokoyama et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Effect of microbial transglutaminase on the rheological and textural characteristics of black soybean packed tofu coagulating with Agar

(2010) Yung-Ho Chang et al.   LWT-FOOD SCIENCE AND TECHNOLOGY

Role of conformational sampling in computing mutation-induced changes in protein structure and stability

(2010) Elizabeth H. Kellogg et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Cross-linking of β-casein by Trichoderma reesei tyrosinase and Streptoverticillium mobaraense transglutaminase followed by SEC–MALLS

(2009) Evanthia Monogioudi et al.   FOOD HYDROCOLLOIDS

Affinity of Microbial Transglutaminase to αs1-, β-, and Acid Casein under Atmospheric and High Pressure Conditions

(2009) Orquídea Menéndez et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Substrate specificity of microbial transglutaminase as revealed by three-dimensional docking simulation and mutagenesis

(2009) U. Tagami et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Secretion of Streptomyces mobaraensis pro-transglutaminase by coryneform bacteria

(2008) Hiroshi Itaya et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Overproduction of soluble recombinant transglutaminase from Streptomyces netropsis in Escherichia coli

(2008) Yu-Jen Yu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Purification and activation of a recombinant histidine-tagged pro-transglutaminase after soluble expression in Escherichia coli and partial characterization of the active enzyme

(2008) Christian K. Marx et al.   ENZYME AND MICROBIAL TECHNOLOGY

Proline Effect on the Thermostability and Slow Unfolding of a Hyperthermophilic Protein

(2008) K. Takano et al.   JOURNAL OF BIOCHEMISTRY

Random mutagenesis of a recombinant microbial transglutaminase for the generation of thermostable and heat-sensitive variants

(2008) Christian K. Marx et al.   JOURNAL OF BIOTECHNOLOGY