期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 24, 页码 -出版社
MDPI
DOI: 10.3390/ijms222413191
关键词
Epichloe gansuensis; symbiosis; full-length transcriptome; differential expression; NaCl tolerance
资金
- Natural Science Foundation of China [32001399, U1812401]
- Karst Science Research Center of Guizhou Province [U1812401]
- Changjiang Scholars and innovative Research Team in University [IRT_17R50]
- Fundamental Research Funds for the Central Universities in Lanzhou University [lzujbky-2021ey01, lzujbky-2021-kb12]
- Open Project of State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University [2021-KF-02]
- Lanzhou University Double First-Class guiding special project-team construction fund-scientific research start-up fee standard [561119206]
- Technical service agreement on research and development of beneficial microbial agents for Alpine Rhododendron [071200001]
- Natural Science Foundation of Qinghai Science & Technology Department [2017-ZJ-950Q]
The seed-borne endophyte Epichloe gansuensis enhances NaCl tolerance in Achnatherum inebrians and increases its biomass by modulating the full-length transcriptome information and gene expression of the host grass.
Seed-borne endophyte Epichloe gansuensis enhance NaCl tolerance in Achnatherum inebrians and increase its biomass. However, the molecular mechanism by which E. gansuensis increases the tolerance of host grasses to NaCl stress is unclear. Hence, we firstly explored the full-length transcriptome information of A. inebrians by PacBio RS II. In this work, we obtained 738,588 full-length non-chimeric reads, 36,105 transcript sequences and 27,202 complete CDSs from A. inebrians. We identified 3558 transcription factors (TFs), 15,945 simple sequence repeats and 963 long non-coding RNAs of A. inebrians. The present results show that 2464 and 1817 genes were differentially expressed by E. gansuensis in the leaves of E+ and E- plants at 0 mM and 200 mM NaCl concentrations, respectively. In addition, NaCl stress significantly regulated 4919 DEGs and 502 DEGs in the leaves of E+ and E- plants, respectively. Transcripts associated with photosynthesis, plant hormone signal transduction, amino acids metabolism, flavonoid biosynthetic process and WRKY TFs were differentially expressed by E. gansuensis; importantly, E. gansuensis up-regulated biology processes (brassinosteroid biosynthesis, oxidation-reduction, cellular calcium ion homeostasis, carotene biosynthesis, positive regulation of proteasomal ubiquitin-dependent protein catabolism and proanthocyanidin biosynthesis) of host grass under NaCl stress, which indicated an increase in the ability of host grasses' adaptation to NaCl stress. In conclusion, our study demonstrates the molecular mechanism for E. gansuensis to increase the tolerance to salt stress in the host, which provides a theoretical basis for the molecular breed to create salt-tolerant forage with endophytes.
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