Review
Biology
Juan A. Miralles de Imperial-Ollero, Alejandro Gallego-Ortega, Arturo Ortin-Martinez, Maria Paz Villegas-Perez, Francisco J. Valiente-Soriano, Manuel Vidal-Sanz
Summary: Phototoxicity animal models, particularly with blue LED exposure, have shown focal damage in the retina and reduced cone population, with potential neuroprotective strategies such as administration of trophic factors or drugs to enhance cell survival.
Article
Immunology
Xin Li, Banglian Hu, Xiaoyan Guan, Ziwei Wang, Yuhang Zhou, Hao Sun, Xian Zhang, Yanfang Li, Xiaohua Huang, Yingjun Zhao, Xin Wang, Huaxi Xu, Yun-Wu Zhang, Zhanxiang Wang, Honghua Zheng
Summary: In this study, researchers used CRISPR/Cas9 genome editing technology to generate a mouse model with CSF1R gene haploinsufficiency, to investigate the pathogenic mechanism of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) and the therapeutic effects of minocycline. The study found that CSF1R deficiency leads to aberrant microglial activation, and minocycline can suppress this activation and improve behavioral and pathological defects.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Biochemistry & Molecular Biology
Rosario Amato, Alessio Canovai, Alberto Melecchi, Salvatore Pezzino, Roberta Corsaro, Massimo Dal Monte, Dario Rusciano, Paola Bagnoli, Maurizio Cammalleri
Summary: Studies have shown that the combination of lutein and C3G can prevent oxidative stress, inflammation, gliotic and apoptotic responses caused by LD, thus protecting photoreceptor cells more effectively than each component alone. Furthermore, they can improve dysfunctional electroretinogram and enhance rod and cone photoreceptor responses.
Article
Biochemistry & Molecular Biology
Juan A. Miralles de Imperial-Ollero, Alejandro Gallego-Ortega, Maria Norte-Munoz, Johnny Di Pierdomenico, Jose Manuel Bernal-Garro, Francisco J. Valiente-Soriano, Manuel Vidal-Sanz
Summary: The study found that focal blue light-emitting diode (LED)-induced phototoxicity in adult rats leads to a gradual reduction of the outer retina, loss of S cones, and dynamic activation of microglial cells in the lesioned area.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Una Greferath, Mario Huynh, Andrew Ian Jobling, Kirstan Anne Vessey, Gene Venables, Denver Surrao, Helen Christine O'Neill, Ioannis J. Limnios, Erica Lucy Fletcher
Summary: Retinitis pigmentosa is a group of inherited retinal degenerations characterized by gradual loss of photoreceptors, ultimately leading to irreversible vision loss. This study focused on analyzing the changes in glial, microglial, and photoreceptor cells in different retinal locations in RCS rats, revealing significant differences in photoreceptor loss between the dorsal and ventral retina. The findings suggest that breakdown of the outer limiting membrane may play a role in exacerbating photoreceptor loss in the ventral retina, highlighting the value of using the RCS rat model for sectorial retinitis pigmentosa.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Ana Martinez-Vacas, Johnny Di Pierdomenico, Alejandro Gallego-Ortega, Francisco J. Valiente-Soriano, Manuel Vidal-Sanz, Serge Picaud, Maria Paz Villegas-Perez, Diego Garcia-Ayuso
Summary: The aim of this study was to investigate the neuroprotective effects of taurine administration in dystrophic rats with retinal degeneration. The results showed that taurine treatment increased taurine plasma levels and photoreceptor survival, improved retinal function, reduced inflammation and oxidative stress, and enhanced retinal pigment epithelium function. These findings indicate that taurine has potential neuroprotective effects in retinal degeneration.
Review
Ophthalmology
Ting-Ting Yang, Hui Li, Li-Jie Dong
Summary: Glycolysis plays different roles in retinal vascular endothelium and Muller cells, influencing angiogenesis and visual function maintenance; stabilizing mitochondrial function can be a therapeutic target for retinal degeneration and other diseases.
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
(2021)
Review
Geriatrics & Gerontology
Janos Feher, Agnes Elo, Lilla Istvan, Zoltan Zsolt Nagy, Zsolt Radak, Gianluca Scuderi, Marco Artico, Illes Kovacs
Summary: This review explores the role of microbiota-mitochondria disorders in age-related macular degeneration (AMD). It suggests that the dysregulation of microbiota and mitochondria may be a crucial mechanism in the development of early AMD and proposes new treatment approaches targeting these disorders.
Review
Cell Biology
Li Pan, Kin-Sang Cho, Irvin Yi, Chi-Ho To, Dong Feng Chen, Chi-Wai Do
Summary: This review focuses on the therapeutic potentials of three major active flavonoids extracted from Scutellaria baicalensis Georgi against ischemia-induced neurotoxicity and damage. These compounds have been shown to have promising pharmacological capabilities in preventing cell and tissue damage, and their bioactivity and bioavailability are also discussed. It is hoped that the therapeutic potential of these flavonoids can be utilized as natural treatments for ischemia-induced injuries of the central nervous system (CNS).
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2021)
Article
Ophthalmology
Diego Garcia-Ayuso, Johnny Di Pierdomenico, Esmeralda Moya-Rodriguez, Francisco J. Valiente-Soriano, Caridad Galindo-Romero, Paloma Sobrado-Calvo
Summary: The study found that female gender, online class attendance, longer hours spent in online classes, and contact lens wearers were more likely to experience dry eye symptoms. This suggests that the synchronous hybrid learning environment may increase students' exposure to video display terminals and the prevalence of dry eye symptoms.
CLINICAL AND EXPERIMENTAL OPTOMETRY
(2022)
Meeting Abstract
Ophthalmology
Alejandro Gallego Ortega, Maria Norte Munoz, Juan Antonio Miralles de Imperial Ollero, Francisco Javier Valiente-Soriano, Pedro De La Villa, Manuel Vidal-Sanz
ACTA OPHTHALMOLOGICA
(2022)
Article
Biochemistry & Molecular Biology
Marina Martinez-Carmona, Fernando Lucas-Ruiz, Alejandro Gallego-Ortega, Caridad Galindo-Romero, Maria Norte-Munoz, Maria Jose Gonzalez-Riquelme, Francisco J. Valiente-Soriano, Manuel Vidal-Sanz, Marta Agudo-Barriuso
Summary: Ly6c can serve as a specific marker of mouse vasculature, particularly of the retinal vascular plexuses. In injured retinas, Ly6c expression increases, correlating with vascular formation and classical monocyte/macrophage activation. This study provides a new tool for qualitative and quantitative assessment of vascular changes in health and disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ana Martinez-Vacas, Johnny Di Pierdomenico, Francisco J. Valiente-Soriano, Manuel Vidal-Sanz, Serge Picaud, Maria Paz Villegas-Perez, Diego Garcia-Ayuso
Summary: In this study, we investigated the effects of taurine deficiency on glial cell activation and oxidative stress. The results showed that taurine deficiency caused cell damage in various layers of the retina and increased the susceptibility of photoreceptor outer segments to light damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Ophthalmology
Johnny Di Pierdomenico, Delaney C. M. Henderson, Sara Giammaria, Victoria L. Smith, Alienor J. Jamet, Corey A. Smith, Michele L. Hooper, Balwantray C. Chauhan
Summary: Age and intraocular pressure (IOP) are the two most important risk factors for the development and progression of open-angle glaucoma. This paper provides a review on the evaluation of retinal ganglion cell loss in experimental glaucoma (EG) using serial in vivo imaging. The study compares the effects of elevated IOP on ganglion cell complex thickness and individual RGC dendritic morphology changes in adult and old mice, highlighting the potential of single RGC imaging for understanding RGC loss.
PROGRESS IN RETINAL AND EYE RESEARCH
(2022)
Article
Ophthalmology
Johnny Di Pierdomenico, Alejandro Gallego-Ortega, Ana Martinez-Vacas, David Garcia-Bernal, Manuel Vidal-Sanz, Maria P. Villegas-Perez, Diego Garcia-Ayuso
Summary: Syngeneic bone marrow mononuclear stem cell transplantation can reduce photoreceptor degeneration and have anti-gliotic effects on Muller cells, but does not improve retinal function.
ACTA OPHTHALMOLOGICA
(2022)
Article
Ophthalmology
Diego Garcia-Ayuso, Esmeralda Moya-Rodriguez, Francisco J. Valiente-Soriano, Caridad Galindo-Romero, Paloma Sobrado-Calvo, Johnny Di Pierdomenico
Summary: This study assessed the level of compliance related to contact lens wear among university students in Spain. The results showed that a relatively high percentage of participants displayed some degree of non-compliance with contact lens care and wearing time. The study emphasized the importance of providing accurate and comprehensive contact lens care guidelines and attending follow-up visits.
CONTACT LENS & ANTERIOR EYE
(2022)
Article
Cell & Tissue Engineering
Maria Norte-Munoz, Alejandro Gallego-Ortega, Fernando Lucas-Ruiz, Maria J. Gonzalez-Riquelme, Yazmin Changa-Espinoza, Caridad Galindo-Romero, Peter Ponsaerts, Manuel Vidal-Sanz, David Garcia-Bernal, Marta Agudo-Barriuso
Summary: This study compares the response of the mouse retina to different modes of MSC transplantation and finds that different transplantation modes lead to varying degrees of anatomical and functional changes, with xenotransplants being the most detrimental. This finding is of great importance for preclinical studies.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Maria Jose Gonzalez-Riquelme, Fernando Lucas-Ruiz, Caridad Galindo-Romero, Raquel Boia, Antonio Francisco Ambrosio, Manuel Vidal-Sanz, Ana Raquel Santiago, Marta Agudo-Barriuso
Summary: This study compares retinal organotypic cultures (ROCs) with in vivo models to study retinal ganglion cell (RGC) death and glial activation. The results show that there are significant differences in glial activation between ROCs and in vivo models, with earlier and different morphological activation in ROCs. Additionally, microglial cell density in ROCs is consistently lower than in vivo. ROCs can be used for proof-of-concept studies on neuroprotection, but long-term experiments should be performed in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ophthalmology
He-Yan Li, Li Dong, Xu-Han Shi, Rui-Heng Zhang, Wen-Da Zhou, Hao-Tian Wu, Chu-Yao Yu, Yi-Tong Li, Yi-Fan Li, Jost B. Jonas, Wen-Bin Wei, Yue-Ming Liu
Summary: This study aimed to examine the intraocular tolerability of cetuximab when applied intravitreally, and its effect on axial elongation. The results showed that intravitreal injections of cetuximab could reduce axial elongation in a dose-dependent and number of treatment-dependent manner, without causing intraocular toxic effects.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
James D. Cole, John A. McDaniel, Joelle Nilak, Ashley Ban, Carlos Rodriguez, Zuhaad Hameed, Marta Grannonico, Peter A. Netland, Hu Yang, Ignacio Provencio, Xiaorong Liu
Summary: Aniridia is a panocular condition characterized by the loss of iris, caused mainly by mutations in the PAX6 gene. This study assessed the effects of Pax6-haploinsufficiency on retinal morphology and vision in Pax6Sey mice. The results showed elevated intraocular pressure and declining visual acuity in Pax6Sey mice, along with local retinal damage and a possible neuroinflammatory response.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Subao Jiang, Ning Xia, Francesco Buonfiglio, Elsa W. Boehm, Qi Tang, Norbert Pfeiffer, Dominik Olinger, Huige Li, Adrian Gericke
Summary: This study found that a high-fat diet induces endothelial dysfunction in the ophthalmic artery, leading to impaired vascular function in the eye. The high-fat diet triggers oxidative stress and involves RAGE and NOX2.
EXPERIMENTAL EYE RESEARCH
(2024)
Review
Ophthalmology
Mala Upadhyay, Vera L. Bonilha
Summary: The sodium iodate (NaIO3) model is a valuable tool in studying dry AMD and evaluating the retinal structure. Through extensive research, it has been found that the death of RPE cells caused by NaIO3 injection is closely related to vision loss in patients with dry AMD. Therefore, studying NaIO3 can provide insights into the mechanisms of RPE and photoreceptor cell death and contribute to understanding RPE degeneration in AMD.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Vicki Chrysostomou, Katharina C. Bell, Sze Woei Ng, Samyuktha Suresh, Gayathri Karthik, Marion Millet, Yingying Chung, Jonathan G. Crowston
Summary: We present a new experimental model for inducing retinal ganglion cell (RGC) dysfunction and degeneration in mice. This model allows for the reliable induction of RGC dysfunction and loss, with the resilience of the optic nerve being dependent on the age of the animal. The time-defined nature of the functional and structural changes observed in this model will facilitate the study of glial responses and the evaluation of neuroprotective treatments after intraocular pressure (IOP) injury.
EXPERIMENTAL EYE RESEARCH
(2024)