Article
Endocrinology & Metabolism
Pengxiang Tian, Zhiming Zhao, Yanli Fan, Na Cui, Baojun Shi, Guimin Hao
Summary: This study investigated the effects of stress on male reproductive function by observing changes in the testes of rats exposed to stress. It found that chronic stress can lead to pathological changes in the testes, decreased expression of key marker genes related to spermatogenesis, and alterations in the population of spermatogenic cells, which may contribute to male reproductive dysfunction.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Weihao Sun, Xinhui Zhang, Lei Wang, Guanyu Ren, Shuguang Piao, Chenghua Yang, Zhiyong Liu
Summary: The study investigated the histological changes and molecular mechanisms in cryptorchidism patients aged 20 to 40 years. Results showed a significant atrophy and decline in spermatogenic cells in the testes of patients with longer disease duration, with downregulation of genes related to sperm development and differentiation, providing insights into the decline in fertility in adult cryptorchidism.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yongfu La, Xiaoming Ma, Pengjia Bao, Min Chu, Ping Yan, Xian Guo, Chunnian Liang
Summary: This study summarized the changes in piRNA expression patterns during the early development of yak testis and provided new insights into the regulatory role of piRNA in yak testis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Geriatrics & Gerontology
Chenghao Jin, Yijie Shao, Xiaotao Zhang, Jiani Xiang, Ruize Zhang, Zeyu Sun, Shuhao Mei, Jingyi Zhou, Jianmin Zhang, Ligen Shi
Summary: The study investigated the senescence-associated alterations of microglia in the aged brain at the single cell level, revealing six subgroups of microglia with unique functions. Among them, a unique type of highly activated microglia (HAM) was observed only in aged mice. Mechanistically, neuron-released Mif acted through Cd74 receptor in HAM to promote immunochemotactic activity of microglia, triggering immuno-inflammatory responses in aged brains. These findings may provide new targets for reducing age-related brain inflammation and maintaining brain health.
Article
Multidisciplinary Sciences
Wei Zhang, Siyu Xia, Wei Xiao, Yali Song, Li Tang, Min Cao, Jing Yang, Shuang Wang, Zhijie Li, Chengchao Xu, Jianqiao Liu, Shanchao Zhao, Chuanbin Yang, Jigang Wang
Summary: This study focuses on the reproductive issues of advanced paternal age and analyzes the molecular and cellular changes associated with aging in the testes of mice. The study discovers various aging-related biological processes and identifies an increased subtype of aging-specific macrophages that contribute to a proinflammatory environment during testicular aging.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Cell Biology
Pauline Sararols, Isabelle Stevant, Yasmine Neirijnck, Diane Rebourcet, Annalucia Darbey, Michael K. Curley, Francoise Kuhne, Emmanouil Dermitzakis, Lee B. Smith, Serge Nef
Summary: FLCs and ALCs exhibit significant differences in transcriptional and functional features, including gene expression variations and non-transcriptional events such as intron retention. The FLC-specific expression of Crhr1 and Mc2r receptors suggests a dual regulation of steroidogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Jie Su, Yue Zhang, Hong Su, Caiyun Wang, Daqing Wang, Yanyan Yang, Xiunan Li, Wangmei Qi, Haijun Li, Xihe Li, Yongli Song, Guifang Cao
Summary: Male and female mammals have different sex chromosomes, with females carrying XX chromosomes and males carrying XY chromosomes. In sheep testes, there is no dosage compensation mechanism observed, and MSL1 and MSL2 are expressed in early germ cells and involved in regulating mammalian X-chromosome inactivation and activation.
Article
Cell Biology
Daoyuan Huang, Yuesheng Zuo, Chen Zhang, Guoqiang Sun, Ying Jing, Jinghui Lei, Shuai Ma, Shuhui Sun, Huifen Lu, Yusheng Cai, Weiqi Zhang, Fei Gao, Andy Peng Xiang, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Jing Qu, Si Wang
Summary: Our study provides an in-depth understanding of the cellular and molecular changes in non-human primate testicular aging through the establishment of a single-cell transcriptomic atlas. We identified Sertoli cells as the most susceptible to aging and found that the downregulation of the transcription factor WT1 is associated with accelerated cellular senescence and the creation of a hostile microenvironment.
Article
Cell Biology
Xia Chen, Yujuan Qi, Qiuru Huang, Chi Sun, Yanli Zheng, Li Ji, Yi Shi, Xinmeng Cheng, Zhenbei Li, Sen Zheng, Yijuan Cao, Zhifeng Gu, Jun Yu
Summary: This study used single-cell RNA sequencing analysis to characterize the transcriptomes of terminal epithelium in the testis of Drosophila during aging. It revealed the functional characteristics and regulatory mechanisms of terminal epithelium populations, as well as age-related genes and their evolutionary advantages. These findings provide a gene target resource for further research on the functions of testicular terminal epithelium during aging.
Article
Oncology
Soazik P. Jamin, Feria Hikmet, Romain Mathieu, Bernard Jegou, Cecilia Lindskog, Frederic Chalmel, Michael Primig
Summary: CT genes are induced in germ cells and play a role in cancer progression. Identifying these genes is challenging due to the diverse sources and time periods of data. A study using RNA profiling analysis identified 478 candidate loci, including known CT genes and novel candidates that may serve as potential therapeutic targets or biomarkers.
MOLECULAR ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Justine Saulnier, Frederic Chalmel, Marion Delessard, Laura Moutard, Tony Pereira, Francois Fraissinet, Ludovic Dumont, Aurelie Rives-Feraille, Christine Rondanino, Nathalie Rives
Summary: In vitro spermatogenesis is a promising approach to restore the fertility of childhood cancer survivors, but faces challenges in the rat model due to germ cell maturation arrest. The study revealed deregulation of several genes in vitro matured rat prepubertal testicular tissues, potentially attributed to defective BTB function, altered steroidogenic pathway, and inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Ya-Dan Zhao, Cai-Xia Yang, Zhi-Qiang Du
Summary: This study compared the process of spermiogenesis between humans and pigs through integrative analysis and identified specific genes and molecular pathways for each type of sperm. The findings provide important resources and insights for further investigation on sperm maturation and male reproductive ability.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Review
Biochemistry & Molecular Biology
Fan Dong, Ping Ping, Yi Ma, Xiang-Feng Chen
Summary: This study provides a comprehensive review of the application of single-cell RNA sequencing (scRNA-seq) in the human testis. ScRNA-seq has revealed dynamic transcriptional changes in germ cells and somatic cells during testicular development from embryos to adults. It has also identified abnormalities in metabolic signaling and hedgehog signaling in aged somatic cells and Leydig cells, respectively. Furthermore, scRNA-seq has uncovered profound changes in testes of infertile males and made additional discoveries through the re-analysis of public scRNA-seq data, such as the potential vulnerability of testicular SARS-CoV-2 infection and evolutionary conservatism and divergence among species.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Cell Biology
Yun-Hua Ji, Lin-Meng Wang, Fu-Xun Zhang, Hao-Zhong Hou, Zhi-Rong Luo, Qi Xue, Man-Man Shi, Yong Jiao, Dong Cui, Da-Li He, Wei Xue, Yu-qi Wen, Qi-Sheng Tang, Bo Zhang
Summary: This study revealed the regulatory effects of hypobaric hypoxia exposure on germ cell development and sperm differentiation using single-cell RNA sequencing. It also identified the mechanisms of damage caused by hypobaric hypoxia.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Hanbo Jia, Wei Wang, Zhaowen Zhou, Zhiyi Chen, Zijun Lan, Hao Bo, Liqing Fan
Summary: Spermatogenesis, the process of sperm production, is regulated by various key cells and any abnormalities can lead to male infertility-related diseases. Single-cell RNA sequencing technology is being increasingly used in male reproduction for its unique insights. This technology covers different stages and diseases related to male infertility, providing valuable research results and application directions. Challenges and prospects of combining this technology with other techniques are also discussed.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Environmental Sciences
Siyu Li, Xiaoyan Zheng, Xiaoya Zhang, Hongxiang Yu, Bing Han, Yueying Lv, Yan Liu, Xiaoqiao Wang, Zhigang Zhang
Summary: Exposure to Deltamethrin induces hepatic steatosis, oxidative stress, inflammation, and apoptosis in quails. The Nrf2/TGF-beta 1/Smad3 signaling pathway plays a crucial role in DLM-induced liver fibrosis, and the addition of resveratrol can alleviate this fibrosis by activating Nrf2 expression.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Multidisciplinary Sciences
Qingyue Yang, Bing Han, Siyu Li, Xiaoqiao Wang, Pengfei Wu, Yan Liu, Jiayi Li, Biqi Han, Ning Deng, Zhigang Zhang
Summary: This study demonstrates that Cr(VI) induces liver injury in rats by inhibiting the deacetylation of Sirt1, leading to inflammatory response and apoptosis in hepatocytes. When administered Res as an activator of Sirt1, the deacetylation of Sirt1 is enhanced, resulting in alleviation of inflammatory response and apoptosis.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Biqi Han, Zhanjun Lv, Xuemin Han, Siyu Li, Bing Han, Qingyue Yang, Xiaoqiao Wang, Pengfei Wu, Jiayi Li, Ning Deng, Zhigang Zhang
Summary: Research has shown that HgCl2 can decrease the cell viability and induce oxidative stress in HEK-293 T cells through promoting mitochondrial fission while downregulating the expression of the Sirt1/PGC-1 alpha signaling pathway, leading to mitochondrial disorder. Therefore, Sirt1/PGC-1 alpha may be a promising pharmaceutical target for treating HgCl2-induced kidney injury.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Environmental Sciences
Jiayi Li, Huijie Jiang, Pengfei Wu, Siyu Li, Bing Han, Qingyue Yang, Xiaoqiao Wang, Biqi Han, Ning Deng, Bing Qu, Zhigang Zhang
Summary: Chronic exposure to deltamethrin induces inflammation and apoptosis in quail cerebrums through oxidative stress linked to inhibition of the Nrf2/TLR4 signaling pathway.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biochemistry & Molecular Biology
Xinyue Xu, Zhongxian Yu, Biqi Han, Siyu Li, Yingshuo Sun, Yu Du, Ziwei Wang, Di Gao, Zhigang Zhang
Summary: Our study found that Luteolin can attenuate kidney injury induced by inorganic mercury (HgCl2) by activating the AMPK/mTOR autophagy pathway. Luteolin reduces the accumulation of HgCl2 in the kidneys and promotes its excretion through urine.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2021)
Article
Environmental Sciences
Xiaoqiao Wang, Zhanjun Lv, Bing Han, Siyu Li, Qingyue Yang, Pengfei Wu, Jiayi Li, Biqi Han, Ning Deng, Zhigang Zhang
Summary: The study aimed to explore the adverse effects and potential mechanisms of DBP exposure on rat lungs, finding that DBP promoted inflammatory damage, increased apoptosis and oxidative stress, and upregulated inflammatory cytokines. Conversely, sulforaphane ameliorated the pulmonary damage induced by DBP.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Siyu Li, Pengfei Wu, Bing Han, Qingyue Yang, Xiaoqiao Wang, Jiayi Li, Ning Deng, Biqi Han, Yuge Liao, Yan Liu, Zhigang Zhang
Summary: This study investigated the effect of DLM on quail cerebrum neurons and found that DLM exposure induced histological changes, oxidative stress, and neurons apoptosis in quail cerebrum tissues. Furthermore, the study showed that DLM exposure also increased the expression of ER stress-related mRNA and decreased ATP content in quail cerebrum tissues, indicating ER stress and mitochondrial dysfunction as possible mechanisms for DLM-induced apoptosis of quail cerebrum neurons.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Neurosciences
Jiayi Li, Zhongxian Yu, Bing Han, Siyu Li, Yueying Lv, Xiaoqiao Wang, Qingyue Yang, Pengfei Wu, Yuge Liao, Bing Qu, Zhigang Zhang
Summary: This study investigated the therapeutic potential of selenium yeast (SY) on DLM-induced cerebral injury in quails and found that SY supplementation could alleviate DLM-induced cerebral toxicity by positively regulating the GPX4/TLR4 signaling pathway.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Environmental Sciences
Xue Yang, Yi Fang, Jianbo Hou, Xuejiao Wang, Jiayi Li, Siyu Li, Xiaoyan Zheng, Yan Liu, Zhigang Zhang
Summary: As a synthetic pyrethroid pesticide, deltamethrin (DLM) is widely used in veterinary medicine and farming. DLM-induced oxidative stress causes harm to organisms. The purpose of this research was to study the impact and molecular mechanism of DLM on inflammation and apoptosis in quail cardiomyocytes through the Nrf2/HO-1 signaling pathway. The results showed that DLM induced dose-dependent cardiomyocyte injury by weakening antioxidant defense through down-regulating Nrf2 and HO-1. Furthermore, DLM stimulation induced apoptosis in quail heart and increased inflammation markers.
Article
Environmental Sciences
Ning Deng, Yueying Lv, Qizheng Bing, Siyu Li, Bing Han, Huijie Jiang, Qingyue Yang, Xiaoqiao Wang, Pengfei Wu, Yan Liu, Zhigang Zhang
Summary: Imidacloprid, a widely used insecticide, not only affects target pests but also has negative effects on birds and other creatures. A study on Japanese quails revealed that IMI induces liver fibrosis and inhibits the Nrf2 pathway, which is crucial for the development of fibrosis.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Engineering, Environmental
Biqi Han, Jiayi Li, Siyu Li, Yan Liu, Zhigang Zhang
Summary: This study found that exposure to THI exacerbated liver injury in quails through the microbiota-gut-liver axis, leading to severe damage, fibrosis, and steatosis in the liver.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Yan Liu, Xinyu Guo, Lu Yu, Yuxiang Huang, Changming Guo, Siyu Li, Xu Yang, Zhigang Zhang
Summary: The study found that luteolin can significantly alleviate liver injury caused by mercuric chloride in quails, reduce oxidative stress and inflammation, and promote the excretion of mercury ions.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Ruonan Wang, Yuxiang Huang, Lu Yu, Siyu Li, Jiayi Li, Biqi Han, Xiaoyan Zheng, Zhigang Zhang
Summary: This study investigates the molecular mechanism of testicular toxicity induced by Cr(VI) exposure. The results demonstrate that Cr(VI) exposure disrupts mitochondrial dynamics and induces oxidative stress, leading to testis apoptosis and autophagy. The suppression of Sirt1/Pgc-1α pathway and downregulation of Nrf2 contribute to the observed effects.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Food Science & Technology
Xiaoyan Zheng, Changming Guo, Zhanjun Lv, Huijie Jiang, Siyu Li, Lu Yu, Zhigang Zhang
Summary: Lead (Pb) is widely present in soil and poses a serious threat to agricultural soil and food crops. This study established animal and cell models to investigate the relationship between Pb-induced testicular injury and pyroptosis-mediated fibrosis. The results showed that Pb caused oxidative stress and increased the expression of inflammation, pyroptosis, and fibrosis-related proteins in the testis. In vitro experiments also demonstrated that Pb induced cell damage and elevated reactive oxygen species levels in TM4 Sertoli cells.
FOOD AND CHEMICAL TOXICOLOGY
(2023)