Article
Biochemistry & Molecular Biology
Antonio Augusto Fonseca, Mateus Laguardia-Nascimento, Amanda Petrina Scota Ferreira, Carla do Amaral Pinto, Valdenia Lopes da Silva Goncalves, Aline Aparecida Silva Barbosa, Anselmo Vasconcelos Rivetti, Marcelo Fernandes Camargos
Summary: The objective of this study was to differentiate and correctly diagnose red sea bream iridovirus (RSIV) and infectious spleen and kidney necrosis virus (ISKNV) in fish farming. The sequencing of ORF450L, ORF342L, ORF077 and the intergenic region between ORF37 and ORF42R showed that ORF077 separated the three major clades of Megalocytivirus (MCV), while ISKNV could not be differentiated. All qPCRs tests showed acceptable repeatability values, less than 5%. Therefore, two ISKNV qPCRs and two RSIV qPCRs are considered suitable for the diagnosis and typing of MCV.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Kaiping Deng, Shizhen Steven Wang, Shannon Kiener, Emily Smith, Kai -Shun Chen, Ruiqing Pamboukian, Anna Laasri, Catalina Pelaez, Jodie Ulaszek, Matthew Kmet, Antonio De Jesus, Thomas Hammack, Ravinder Reddy, Hua Wang
Summary: The FDA has developed a rapid qPCR method for detecting Salmonella in food, which has been shown to be reproducible and sensitive.
Article
Multidisciplinary Sciences
Samantha A. A. Koda, Kuttichantran Subramaniam, Paul M. M. Hick, Evelyn Hall, Thomas B. B. Waltzek, Joy A. A. Becker
Summary: A newly developed TaqMan qPCR assay was able to detect different genotypes of ISKNV with high sensitivity and specificity. This assay can be used for surveillance and diagnosis of ISKNV in the aquaculture and ornamental fish industries.
Article
Agriculture, Dairy & Animal Science
Khalid Shahin, Kuttichantran Subramaniam, Alvin C. Camus, Zeinab Yazdi, Susan Yun, Samantha A. Koda, Thomas B. Waltzek, Felipe Pierezan, Ruixue Hu, Esteban Soto
Summary: The study describes an epizootic in tilapia fingerlings in Southern California, USA, and reports the discovery of a novel megalocytivirus associated with the mortalities. The disease in the affected fish presented various systemic symptoms and organ lesions.
Article
Immunology
Prem Perumal, Mohamed Bilal Abdullatif, Harriet N. Garlant, Isobella Honeyborne, Marc Lipman, Timothy D. McHugh, Jo Southern, Ronan Breen, George Santis, Kalaiarasan Ellappan, Saka Vinod Kumar, Harish Belgode, Ibrahim Abubakar, Sanjeev Sinha, Seshadri S. Vasan, Noyal Joseph, Karen E. Kempsell
Summary: This study focused on validating mRNA biomarkers identified in a non-human primate TB model in new patient cohorts. The biomarkers showed good performance characteristics in diagnosing ATB and LTBI, and identifying high-risk LTBI individuals for progression, with potential for low-cost rapid diagnostic solutions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Plant Sciences
Leandro Francisco de Oliveira, Amanda Rusiska Piovezani, Dimitre A. Ivanov, Leonardo Yoshida, Eny Iochevet Segal Floh, Massuo Jorge Kato
Summary: This study identified stable reference genes for gene expression analysis in four Piper species and provided a list of recommended gene pairs for each species. These findings will facilitate further transcriptomic and functional gene research.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Fisheries
Hetong Zhang, Hemei Qi, Shaoping Weng, Jianguo He, Chuanfu Dong
Summary: In this study, the virulence-related gene ORF71L of Infectious spleen and kidney necrosis virus (ISKNV) was identified. Deletion of ORF71L resulted in decreased viral replication rate, reduced virulence, and lower mortality in mandarin fish. Additionally, deletion of ORF71L also led to an increase in the number of melanomacrophage centers in the spleen of infected fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Plant Sciences
Maria Noelia Ulrich, Esteban Muniz-Padilla, Alejandra Corach, Esteban Hopp, Daniela Tosto
Summary: This study evaluated the stability of different reference genes of glyphosate-resistant S. halepense, finding that PP2A and ARI8 were the most stable genes. The results provide valuable information for gene expression analysis under glyphosate stress and will facilitate resistance mechanism studies in this weed species.
Article
Microbiology
Antonio Augusto Fonseca Junior, Carla Amaral Pinto, Cid Aristoteles de Siqueira Alencar, Bruna Lopes Bueno, Jenner K. Pimenta dos Reis, Mauricio Baltazar de Carvalho Filho
Summary: In this study, three qPCR protocols for Burkholderia mallei were compared, showing high efficiency and good repeatability overall. However, reactions with Cq values between 36 and 40 were considered suspicious and unreliable, indicating a need for greater clinical criteria in result analysis.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Katarzyna Linkowska, Tomasz Bogiel, Katarzyna Lamperska, Andrzej Marszalek, Jaroslaw Starzynski, Lukasz Szylberg, Aleksandra Szwed-Kowalska, Malgorzata Pawlowska, Tomasz Grzybowski
Summary: Although SARS CoV-2 infection may not be as serious a threat as before, the increased transmissibility of the Omicron variant remains a challenge for health care systems. This study evaluates the performance of three SARS-CoV-2 diagnostic tests and finds that all have high specificity and sensitivity for detecting the virus. The LOD values for each test were determined and suggest that the reported outcomes should be based on internal validation rather than manufacturer's declarations.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Qian Wu, Xianhui Ning, Li Sun
Summary: The study revealed significant changes in miRNA expression during megalocytivirus infection, with a complex regulatory network formed between miRNAs and their target mRNAs. Additionally, miRNAs and circRNAs involved in regulating immune gene expression during viral infection were identified, highlighting their role in antiviral immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Amanda M. Naaum, Marine Cusa, Maleeka Singh, Zoe Bleicher, Christopher Elliott, Ian B. Goodhead, Robert H. Hanner, Sarah J. Helyar, Stefano Mariani, John E. Rice, Lawrence J. Wangh, J. Aquiles Sanchez
Summary: Seafood accounts for 20% of animal protein consumption globally and is a crucial dietary and income resource for the world population, however, the industry faces challenges such as unsustainable fishing and fraud. FASTFISH-ID is an innovative commercial platform that allows rapid and automated species authentication, offering a promising solution to combat seafood fraud.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Chaofan Jiang, Lin Zhou, Qingchen Zhao, Mengke Wang, Sirui Shen, Te Zhao, Kaidi Cui, Leiming He
Summary: This study validated the expression stability of seven candidate reference genes in Sclerotium rolfsii under different conditions and recommended suitable reference gene combinations for RT-qPCR normalization. The results showed that using one or two reference genes to normalize the expression of target genes in S. rolfsii did not significantly differ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Jiao Chen, Yunjian Wang, Zhi Yang, Danwen Liu, Yao Jin, Xixi Li, Yuhang Deng, Boya Wang, Zhifeng Zhang, Yubin Ma
Summary: This study identified suitable reference genes in U. unicinctus based on transcriptome data and discovered some new stable reference genes. The findings provide important information for future gene expression studies in U. unicinctus.
Article
Fisheries
Kyung-Ho Kim, Kwang-Min Choi, Gyoungsik Kang, Won-Sik Woo, Min-Young Sohn, Ha-Jeong Son, Dongbin Yun, Do-Hyung Kim, Chan-Il Park
Summary: The diagnostic performance of a TaqMan-based real-time PCR assay for detecting red sea bream iridovirus (RSIV) was evaluated. The assay showed rapid and accurate detection of RSIV, with high sensitivity and specificity.
