Article
Zoology
Yu-Chen Ding, Sih-Shien Lee, Sheng-Kai Peng, Wen-Kai Yang, Tsung-Han Lee
Summary: This study observed the osmoregulatory responses of Asian sea bass at different salinities, and found that the lowest osmoregulatory response occurred in the 10 parts per thousand brackish water, with the lowest density of ionocytes and Na+/K+-ATPase required to maintain osmolality.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
(2023)
Review
Cell Biology
Andree-Anne Marcoux, Laurence E. Tremblay, Samira Slimani, Marie-Jeanne Fiola, Fabrice Mac-Way, Alexandre P. Garneau, Paul Isenring
Summary: NKCC2 is an integral membrane protein that mediates the cotranslocation of Na+, K+, and Cl(-) ions in the TALH, allowing for the reclamation of a large fraction of filtered Na+, Cl-, Ca2+, Mg-2(+), and HCO(3)(-). Malfunction of NKCC2 can lead to various inherited disorders, illustrating its physiological importance.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Neurosciences
Abhishek Pethe, Mira Hamze, Marina Giannaki, Bernd Heimrich, Igor Medina, Anna-Maria Hartmann, Eleni Roussa
Summary: This study investigates the effects of KCC2/NBCe1 interaction on the phosphorylation pattern and function of KCC2. The results show that KCC2 and NBCe1 interact in the mouse brain and this interaction may have pathophysiological relevance.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Fisheries
Morgana Tais Streck Marx, Carine de Freitas Souza, Ana Paula Gottlieb Almeida, Sharine Nunes Descovi, Adriane Erbice Bianchini, Juan Antonio Martos-Sitcha, Gonzalo Martinez-Rodriguez, Alfredo Quites Antoniazzi, Bernardo Baldisserotto
Summary: The gills of silver catfish show greater responses to pH changes than the kidneys. H+-ATPase activity in the gills increased in alkaline water, while the expression of atp1a1 gene and NKA activity changed. Genes such as slc9a3 and slc12a2 play significant roles in ion uptake at acidic pH.
Article
Ecology
Deena A. Hansen, Amanda S. Williard, Frederick S. Scharf
Summary: By studying the thermal sensitivity of red drum gill Na+/K++ ATPase, we gained insight into the potential temperature effects on osmoregulatory capabilities and overwinter survival of red drum.
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
(2022)
Article
Cell Biology
Jennyfers Chong-Robles, Ivone Giffard-Mena, Araceli Patron-Soberano, Guy Charmantier, Viviane Boulo, Deyanira Rodarte-Venegas
Summary: The cellular function of specific cells in the osmoregulatory structures of branchial chambers in decapod crustaceans has not been well studied. This study found that osmoregulatory capabilities in Litopenaeus vannamei were gradually established during the development of branchiostegites and epipodites, but not gills. The cellular structures observed during the larval phase do not have the typical ultrastructure of ionocytes, indicating that pleura, branchiostegites, or bud gills do not participate in osmoregulation.
CELL AND TISSUE RESEARCH
(2022)
Article
Peripheral Vascular Disease
Chuanming Xu, Yanting Chen, Fei Wang, Shiying Xie, Tianxin Yang
Summary: This study reveals a novel mechanism by which PRR and sPRR negatively regulate NCC activity through S1P, impacting renal excretion of salt and potassium and thus modulating blood pressure and potassium balance.
Article
Physiology
Xin-Peng Duan, Peng Wu, Dan-Dan Zhang, Zhong-Xiuzi Gao, Yu Xiao, Evan C. Ray, Wen-Hui Wang, Dao-Hong Lin
Summary: Kir5.1 plays a significant role in mediating the effect of high sodium intake on basolateral K+ channels in the distal convoluted tubule and Na+-Cl- cotransporter activity/expression, and is involved in maintaining renal ability of K+ excretion during high sodium intake.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Nur Farah Meor Azlan, Maarten P. Koeners, Jinwei Zhang
Summary: Hypertension, the leading cause of cardiovascular disease mortality worldwide, has been linked to alterations in the NCC and its regulatory mechanisms. Recent research has revealed significant progress in understanding the control of NCC, potentially identifying novel therapeutic targets for hypertension.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Biochemistry & Molecular Biology
Marty Kwok Shing Wong, Yousuke Tsuneoka, Takehiro Tsukada
Summary: The expression patterns of NKA & alpha;1-isoforms in the gill of chum salmon acclimated to freshwater and seawater were examined using in situ hybridization chain reaction. The results showed that the NKA & alpha;1c isoform was ubiquitously expressed in gill tissue and not affected by salinity. In freshwater, lamellar ionocytes expressed cytoplasmic NKA & alpha;1a and nuclear NKA & alpha;1b transcripts, while in seawater, filament ionocytes expressed cytoplasmic NKA & alpha;1b as the major isoform. The study also demonstrated the plasticity of ionocyte populations in chum salmon, indicating their ability to switch ionocyte types in response to salinity demands.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
A. Barany, C. A. Shaughnessy, R. M. Pelis, J. Fuentes, J. M. Mancera, S. D. McCormick
Summary: Two orthologues of the gene encoding the Na+-Cl- cotransporter (NCC), termed ncca and nccb, were found in the sea lamprey genome. The NCCs occupied basal positions within the NCC clades in a phylogenetic comparison among other vertebrate NCC and NKCC sequences. nccb is likely the key ion cotransport protein for ion uptake by the lamprey intestine that facilitates water absorption in seawater.
SCIENTIFIC REPORTS
(2021)
Article
Peripheral Vascular Disease
Zhong-Xiuzi Gao, Qi-Chao Wei, Ting-Ting Shu, Shu-Ting Li, Rui Zhou, Ming-Yan Li, Zi-Hui Mao, Dong-Wei Liu, Zhang-Suo Liu, Peng Wu
Summary: This study found that high salt intake in early streptozotocin-induced diabetes leads to imbalanced Na+/K+ ratio, increased blood pressure, and enhanced NCC activity. These effects are associated with increased expression and activity of Kir4.1 in the distal convoluted tubule (DCT). Additionally, the study suggests that Kir4.1 deletion prevents the upregulation of NCC and the development of salt-sensitive hypertension.
JOURNAL OF HYPERTENSION
(2023)
Article
Ecology
Phurich Boonsanit, Supanut Pairohakul
Summary: The mud crab S. olivacea is well-adapted to salinity changes in intertidal mangroves, evidenced by its osmoregulatory and antioxidant responses. It exhibits hyper-hypoosmoregulation, with significant effects of salinity on Na+/K+ ATPase and antioxidant enzyme activities in posterior gills. An increase in gill SOD activities in response to salinity stress may help cope with elevated ROS levels due to high metabolic demands.
MARINE BIOLOGY RESEARCH
(2021)
Article
Neurosciences
Jesper Edvardsson Rasmussen, Patrik Lundstrom, Per Olof Eriksson, Helge Rask-Andersen, Wei Liu, Goran Laurell
Summary: This study aimed to understand the acute effects of furosemide in the inner ear, including protein localization. The results showed rapid changes in NKCC1 induced by furosemide, and an increase in staining intensity of Fetuin-A and PEDF, possibly as an otoprotective response.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Critical Care Medicine
Man Li, Junqiao Lv, Zhiqiang Wang, Liping Wang, Zhixin Qin, Chen Deng, Jinming Liu, Lin Sun
Summary: HMGB1 promotes the expression of NKCC1 and NHE1 in astrocytes and endothelial cells through activation of the HMGB1-TLR4-TRIF-NF-kappa B signaling pathway, leading to cytotoxic and ionic edema following spinal cord injury. Inhibition of HMGB1 reduces the expression of NKCC1 and NHE1 and decreases spinal cord edema. These findings are important for understanding the regulation of spinal cord edema after injury.
JOURNAL OF NEUROTRAUMA
(2023)
Article
Endocrinology & Metabolism
Jonathan D. Douros, David A. Baltzegar, Jamie Mankiewicz, Jordan Taylor, Yoko Yamaguchi, Darren T. Lerner, Andre P. Seale, E. Gordon Grau, Jason P. Breves, Russell J. Borski
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2017)
Article
Endocrinology & Metabolism
Jason P. Breves, Paige L. K. Keith, Bethany L. Hunt, K. Keano Pavlosky, Mayu Inokuchi, Yoko Yamaguchi, Darren T. Lerner, Andre P. Seale, E. Gordon Grau
JOURNAL OF MOLECULAR ENDOCRINOLOGY
(2017)
Article
Endocrinology & Metabolism
Yoko Yamaguchi, Jason P. Breves, Maria C. Haws, Darren T. Lerner, E. Gordon Grau, Andre P. Seale
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2018)
Article
Endocrinology & Metabolism
Jonathan D. Douros, David A. Baltzegar, Benjamin J. Reading, Andre P. Seale, Darren T. Lerner, E. Gordon Grau, Russell J. Borski
FRONTIERS IN ENDOCRINOLOGY
(2018)
Article
Biochemistry & Molecular Biology
K. Keano Pavlosky, Yoko Yamaguchi, Darren T. Lerner, Andre P. Seale
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2019)
Article
Physiology
Andre P. Seale, K. Keano Pavlosky, Fritzie T. Celino-Brady, Yoko Yamaguchi, Jason P. Breves, Darren T. Lerner
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY
(2019)
Article
Fisheries
Andre P. Seale, K. Keano Pavlosky, Fritzie T. Celino-Brady, Darren T. Lerner
Article
Marine & Freshwater Biology
Fritzie T. Celino-Brady, Cody K. Petro-Sakuma, Jason P. Breves, Darren T. Lerner, Andre P. Seale
AQUATIC TOXICOLOGY
(2019)
Article
Endocrinology & Metabolism
Cody Petro-Sakuma, Fritzie T. Celino-Brady, Jason P. Breves, Andre P. Seale
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2020)
Review
Endocrinology & Metabolism
Andre P. Seale, Gardi Hewage Tharindu Malintha, Fritzie T. Celino-Brady, Tony Head, Mahdi Belcaid, Yoko Yamaguchi, Darren T. Lerner, David A. Baltzegar, Russell J. Borski, Zoia R. Stoytcheva, Jason P. Breves
JOURNAL OF NEUROENDOCRINOLOGY
(2020)
Review
Endocrinology & Metabolism
Fritzie T. Celino-Brady, Darren T. Lerner, Andre P. Seale
Summary: Increasing industrial and agricultural activities have led to an increase in pollutant discharges, affecting the growth and reproductive development of vertebrates, particularly fish, through endocrine-disrupting chemicals (EDCs). Fish serve as sentinel species due to their early susceptibility to waterborne EDCs, with studies focusing on acute or chronic exposure at different developmental stages. Various experimental methods are employed to characterize the effects of prevalent EDCs and potential carriers like microplastics, including in vivo, in vitro, and computational approaches.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Cell Biology
Lucia A. Seale, Christy L. Gilman, Ann Marie Zavacki, P. Reed Larsen, Mayu Inokuchi, Jason P. Breves, Andre P. Seale
Summary: The study indicates that thyroid hormones play a crucial role in the adaptation of Mozambique tilapia to freshwater environments by interacting with branchial deiodinases to modulate their activities. External osmotic conditions do not directly affect the gene expression of branchial deiodinases, while systemic signals may stimulate thyroid hormone metabolism.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Article
Physiology
Daniel W. Woo, G. H. T. Malintha, Fritzie T. Celino-Brady, Yoko Yamaguchi, Jason P. Breves, Andre P. Seale
Summary: Prolactin cells in Mozambique tilapia rapidly respond to temperature and osmotic stimuli by releasing different isoforms of prolactin. An increase in temperature stimulates the release of prolactin and causes an increase in cell volume. The mRNA expression related to osmotic stimuli is affected by temperature changes as well.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2022)
Article
Endocrinology & Metabolism
Ryan J. A. Chang, Fritzie T. Celino-Brady, Andre P. Seale
Summary: In estuarine environments, euryhaline fish maintain internal osmolality despite changes in environmental salinity. The neuroendocrine system, specifically the hypothalamic-pituitary-interrenal (HPI) axis, plays a crucial role in this process by releasing cortisol. Cortisol acts as both a mineralocorticoid and glucocorticoid, regulating osmoregulation and metabolism. This study investigated the responses of cortisol, pituitary pomc expression, and corticosteroid receptors in the gill and liver of Mozambique tilapia under different salinity challenges. The results suggest that the HPI-axis is important for salinity acclimation in changing environments.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sang Hyuck Lee, Mi Ae Kim, Young Chang Sohn
Summary: This study characterized the CCAP signaling system in Pacific abalone and revealed its relationship with the calcium/PKC and cAMP/PKA signal transduction pathways. It provides new insights into the evolutionary origins of the NPS and OT/VP signaling systems in protostomes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Hanna Scheuffele, Erica V. Todd, John A. Donald, Timothy D. Clark
Summary: Global warming is causing an increase in extreme weather events, affecting ecosystems and the behavior of ectotherms. More research is needed to understand the impact of diurnal thermal variability and improve climate change predictions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jaakko Haverinen, Ahmed Badr, Markus Eskelinen, Matti Vornanen
Summary: The acclimatization of certain ectothermic vertebrates to winter conditions involves reduced energy consumption, achieved through decreased movement activity, depression of cellular functions, or switching to anaerobic energy production. This study on crucian carp showed that winter-acclimatized fish exhibited lower metabolic rates and a shift to anaerobic energy production during anoxia. Winter dormancy in crucian carp is achieved through active reduction of energy consumption, a slowed metabolic rate, and a direct response to oxygen absence.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Maria Cruz Sueiro, Cynthia A. Awruch, Gustavo M. Somoza, Walter S. Svagelj, Maria G. Palacios
Summary: According to life-history theory, there might be different immunological strategies between reproductive and non-reproductive periods due to limited resources. Our study on two sympatric marine fish species, rockfish and sandperch, found that reproductive individuals showed lower immune function, but higher levels of natural antibodies in rockfish females, compared to their non-reproductive counterparts. On the other hand, reproductively active sandperch males showed lower levels of natural antibodies and higher neutrophil to lymphocyte ratio and spleen index compared to non-reproductive males. The study highlights the species-specific patterns of immunity and the potential influence of resource limitation and abiotic factors on immune trade-offs in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
James B. Cummins, Dane A. Crossley II
Summary: This study focuses on cardiovascular development in altricial bird species, which has been less studied compared to precocial species. The researchers investigated the cholinergic and adrenergic receptor mediated control of arterial blood pressure and heart rate in the neotropic cormorant. The findings suggest that both cholinergic and adrenergic tone play a role in cardiovascular regulation in embryonic birds.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Ru Xia, Hong-Kang Liu, Xi-Feng Liu, Xin Deng, Chuan-Jie Qin, Yuan-Fa He, Shi-Mei Lin, Yong-Jun Chen
Summary: This study cloned the genes encoding glucokinase and glucose-6-phosphatase catalytic subunit in largemouth bass, revealing their distribution and transcriptional regulation. The results showed that the functions of G6pc and Gck in LMB were highly conserved in evolution, and the interconversion of glucose and G6P in the liver was well regulated at the transcript level under high starch diet, but a futile cycle was induced after a glucose load.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)