期刊
CHEMICAL ENGINEERING JOURNAL
卷 437, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2022.135397
关键词
Near-infrared; Polarity; Mitophagy; Bioimaging; Tumor
资金
- National Natural Science Foundation of China [22074084, 21878180]
- One hundred people plan of Shanxi Province, Shanxi Province1331 project key innovation team construction plan cultivation team [2018-CT-1]
- Xiangyuan County Solid Waste Comprehensive Utilization Science and Technology Project [2018XYSDJS-05]
- Shanxi Province Foundation for Returness [2017026]
- Shanxi Collaborative Innovation Center of High Value-added Utilization of Coal-related Wastes [2015-10-B3]
- Innovative Talents of Higher Education Institutions of Shanxi, Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi [2019L0031]
- Key R&D Program of Shanxi Province [201903D421069]
- Shanxi Province Science Foundation [201901D111015]
- Key R&D and transformation plan of Qinghai Province [2020-GX-101]
- Scientific Instrument Center of Shanxi University [201512]
- Shanxi Province Foundation for Selected [2019]
Mitophagy is a process that removes excess or damaged mitochondria and affects cell polarity. A polarity-sensitive fluorescent probe, NIR-BT-P, can monitor mitophagy and distinguish normal cells from cancer cells. The probe was also able to visualize mitophagy in cardiomyocytes during starvation.
Mitophagy is a process that selectively removes excess or damaged mitochondria, and regulates the number of mitochondria in the cell to maintain the normal mitochondrial function. According to reports, mitophagy can cause significant changes to the intracellular microenvironment, such as affecting cell polarity. Therefore, detecting changes in polarity in the intracellular microenvironment is an effective means to monitor the autophagy process. Herein, we synthesized a polarity-sensitive fluorescent probe NIR-BT-P with a strong ICT mechanism and 900 nm near infrared region. The probe was constructed by an onium salt as the electron deficient parent fluorophore and triphenylamine as a strong electron donating group. Studies indicated that the fluorescence intensity of NIR-BT-P increases with decreasing environmental polarity, accompanied by a redshift (812 nm-853 nm) of the maximum emission wavelength. In addition, NIR-BT-P did not interfere by viscosity, pH, or common active substances. Notably, the probe NIR-BT-P can monitor starvation and drug induced mitophagy in real time, and successfully distinguished normal cells from cancer cells. More importantly, by visualizing the cardiomyocytes of the heart of the starvation mice group, it was found that mitophagy occurred during heart starvation. Consequently, we anticipate that NIR-BT-P could not only provide insight into the relationship between mitophagy and heart disease, but may also emerge as a potential tool for the clinical diagnosis of cancer.
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