期刊
CARBOHYDRATE POLYMERS
卷 278, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.118910
关键词
Carboxymethyl chitosan; Berberine hydrochloride; Staphylococcus aureus; Mastitis
资金
- National Natural Science Founda-tion of China [31802091, 31702302]
- Beijing Education Commission Science and Technology Project [SQKM201710020011]
- Research Fund for Academic Degree & Graduate Education of BUA
This study prepared a novel berberine hydrochloride-carboxymethyl chitosan hydrogel (BH-CMCH) with excellent properties and demonstrated its antibacterial activity against Staphylococcus aureus in a rat mastitis model. Proteomics analysis indicated that BH-CMCH enhanced autolysosome degradation and activated the lysosomal pathway to promote the innate immune response.
Staphylococcus aureus (S. aureus) is the major pathogen responsible for mastitis in dairy cows, an important threat to their health, but prevention of S. aureus infection of the mammary gland remains challenging. Berberine hydrochloride (BH), a naturally occurring phytochemical, exhibits a wide range of activities, including anti-bacterial effects on S. aureus. In this study, we prepared a novel berberine hydrochloride-carboxymethyl chitosan hydrogel (BH-CMCH) with excellent thermosensitivity, injectability and in vitro antibacterial activity. In a rat model of mastitis induced by S. aureus, mammary duct injection of BH-CMCH reduced the bacterial load in infected mammary gland tissue and protected the tissue from damage from infection. In addition, proteomics analysis showed that mammary duct injection of BH-CMCH enhanced autolysosome degradation and promoted the innate immune response by activating the lysosomal pathway and up-regulating related significantly differentially expressed proteins (SDEPs). Taken together, the findings support the potential of BH-CMCH as an antibacterial agent against S. aureus-induced mastitis.
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