Structural characterisation and antitumor activity against non-small cell lung cancer of polysaccharides from Sanghuangporus vaninii

The medicinal fungus Sanghuangporus vaninii can be cultivated in large scale and has outstanding antitumour activity. In this study, water-soluble S. vaninii polysaccharides (SVPs) were extracted from fruiting bodies. Four polysaccharide sub-fractions (SVP-W, SVP-1, SVP-2 and SVP-3) were isolated, with molecular weights from 90.50 kDa to 261.70 kDa, and all inhibited the proliferation of non-small cell lung cancer cell lines A549, 95-D and NCI-H460, especially the acidic SVP-1. SVP-1 affected cell morphology and colony formation in NCI-H460 cells. It also promoted cell apoptosis following nuclear fluorescence staining and flow cytometry. Methylation and nuclear magnetic resonance analyses revealed that SVP-1 is a heteroglycan with the main chain -> 4)-beta-DGlcp-(1 -> 6)-beta-D-Glcp-(1 -> 6)-alpha-D-Galp-(1 -> 6)-beta-D-Glcp-(1 ->, and the branched chain alpha-D-Manp-(1 -> 2)-alpha-DManp-(1 -> 3)-beta-D-Glcp-(1 -> 3,6)-beta-D-Glcp-(1 ->. The findings indicate that this natural acidic polysaccharide has potential for non-small cell lung cancer therapy.

Automated summary

Water-soluble polysaccharides (SVPs) were extracted from the medicinal fungus Sanghuangporus vaninii and four polysaccharide sub-fractions were isolated, with SVP-1 showing significant inhibition of non-small cell lung cancer cells and potential for therapy. The findings suggest that SVP-1, a natural acidic polysaccharide, has antitumor activity and induces cell apoptosis in lung cancer cells.