4.8 Article

A smartphone-based electroporation system with highly robust and low-voltage silicon nanopillar chips

期刊

BIOSENSORS & BIOELECTRONICS
卷 197, 期 -, 页码 -

出版社

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113776

关键词

Nanopillar; Electric field enhancement; Electroporation; Low-voltage; Highly robust

资金

  1. Hong Kong RGC CRF grant [C6009-17G]
  2. Guangzhou Science, Technology and Innovation Commission [201704030101]

向作者/读者索取更多资源

This study introduces a novel smartphone-based electroporation system integrated with gold-coated silicon-nanopillar chips for low-voltage EP and ECL. The fabricated chips demonstrated high EP efficiency and cell viability at smaller voltages compared to traditional devices, showcasing potential for various intracellular applications.
In this work, a novel smartphone-based electroporation (EP) system integrated with 3D scalable and robust gold-coated silicon-nanopillar Electroporation (Au-Si NP-EP) chip using projection photolithography is developed, for the first time, for both EP and electric cell lysing (ECL) at low voltages. Au-SiNP-EP chip consists of silicon nanopillars fabricated by using ASML stepper, Deep Reactive Ion Etching (DRIE) process and coated with a gold microelectrode. The silicon nanopillars were optimized based on theoretical analysis and numerical simulations to enhance the electrical field intensity and mechanical strength. The fabricated Au-SiNP-EP chips are tested with both permeable (Acridine Orange (AO) and impermeable (Propidium Iodide (PI)) molecules for HeLa cells at different volts (1-8 V) and pulse duration (1-9 mu s). The fabricated chip achieved an optimized EP efficiency of 84.3% and cell viability of 81.4% at a much smaller voltage (4.5V) than reported planar electroporation (PEP) devices (8-100V). Compared with nanostructures-based devices (2-20 V), our devices show both higher me-chanical strength and fabrication yield. Besides, a smartphone app integrated with a low-cost open-source portable Arduino-based system is developed to provide optimized electrical protocols for both EP and ECL. The electric cell lysing with ECL efficiency of 97.0% at 7 V and pulse duration of 9 ms has been successfully demonstrated. The experimental results show that the proposed smartphone-based EP system with Au-SiNP EP chips is promising for various applications, including intracellular delivery of various biomolecules, drugs, and release of DNA/RNA molecules from biological cells.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Mathematics, Interdisciplinary Applications

MULTI-FACTOR STATUS PREDICTION BY 4D FRACTAL CNN BASED ON REMOTE SENSING IMAGES

Li Wang, Xiaoyi Wang, Zhiyao Zhao, Yuxi Wu, Jiping Xu, Huiyan Zhang, Jiabin Yu, Qian Sun, Yuting Bai

Summary: There are two problems in the current research on algal blooms: the difficulty of considering the impact of other factors on cyanobacteria blooms and the inability to determine the relationship between various factors; and the lack of comprehensive analysis of the whole water area based on field monitoring data. This research proposes a new method for predicting cyanobacteria blooms based on 4D fractal CNN, which uses remote sensing images and their inversion maps to extract features, capture temporal and spatial characteristics and interactions between multiple factors, and accurately predict the eutrophication level of water bodies.

FRACTALS-COMPLEX GEOMETRY PATTERNS AND SCALING IN NATURE AND SOCIETY (2022)

Article Food Science & Technology

Microbiological predictive modeling and risk analysis based on the one-step kinetic integrated Wiener process

Qian Chen, Zhiyao Zhao, Xiaoyi Wang, Ke Xiong, Ce Shi

Summary: The proposed approach based on OS-WP successfully models microbial hazards in food and provides timely decision support for food safety management by accurately predicting microbial risks in food.

INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES (2022)

Article Agronomy

Multi-Chain Collaboration-Based Information Management and Control for the Rice Supply Chain

Xiangzhen Peng, Xin Zhang, Xiaoyi Wang, Haisheng Li, Jiping Xu, Zhiyao Zhao

Summary: This study focuses on the issue of food safety in the rice supply chain. By developing a multi-chain collaborative model and implementing mechanisms such as trusted chains, encryption, and consensus, the study effectively addresses the real-time data interaction problem and ensures trustworthy management and control of information in the rice supply chain. The research provides valuable insights for the digital transformation of the food industry.

AGRICULTURE-BASEL (2022)

Article Business

Sustaining customer loyalty of fresh food e-tailers: an empirical study in China

Li Cui, Shanshan He, Hepu Deng, Xiaoyi Wang

Summary: This study investigates the importance of trust and perceived product risk in sustaining customer loyalty in the fresh food e-commerce industry in China. The study also highlights the significant influence of customer satisfaction on trust. Additionally, the study suggests that the perceived risk of online shopping does not have a significant impact on customer trust and loyalty.

ASIA PACIFIC JOURNAL OF MARKETING AND LOGISTICS (2023)

Article Nanoscience & Nanotechnology

Origami Paper-Based Stretchable Humidity Sensor for Textile- Attachable Wearable Electronics

Xingru Chen, Yongkai Li, Xiaoyi Wang, Hongyu Yu

Summary: This study proposes a novel flexible and stretchable paper-based origami humidity sensor (POHS) that integrates origami folding structures with a paper substrate. The sensor exhibits high sensitivity, good response and recovery properties, and excellent stability during deformation. It has the potential for applications in wearable devices for monitoring respiration rate and diaper wetting detection.

ACS APPLIED MATERIALS & INTERFACES (2022)

Article Engineering, Electrical & Electronic

Sensitivity and Frequency Response Improvement of the Micro Thermal Convective Accelerometer With Structure Optimization

Xiaoyi Wang, Yi-Kuen Lee, Wei Xu

Summary: A theoretical model for transient analysis of the micro thermal convective accelerometer (MTCA) is presented in this study. The model is used to efficiently predict parametric effects compared to time-consuming and complex CFD simulations and experimental testing. The results show that devices with a thin film, small size, and small distance between detectors and microheater achieve a faster frequency response. In addition, guidelines for optimizing sensitivity are provided.

JOURNAL OF MICROELECTROMECHANICAL SYSTEMS (2022)

Article Engineering, Electrical & Electronic

A Flexible Thin-Film Pressure Sensor Based on Thermal Conduction Mechanism for Pressure, Orientation, and Mapping Signal Acquisition

Xiaoyi Wang, Yang Deng, Peng Jiang, Yik Kin Cheung, Zhongyi Liu, Huikai Xie, Hongyu Yu

Summary: In recent years, high-performance flexible pressure sensors have gained popularity and research attention due to their wide applications in various fields. However, it is challenging to achieve low cost and excellent performance simultaneously in flexible sensors.

IEEE SENSORS JOURNAL (2023)

Review Agriculture, Multidisciplinary

A review on blockchain smart contracts in the agri-food industry: Current state, application challenges and future trends

Xiangzhen Peng, Zhiyao Zhao, Xiaoyi Wang, Haisheng Li, Jiping Xu, Xin Zhang

Summary: This research conducts a comprehensive review and content analysis of blockchain smart contract research in the agri-food industry, using bibliometric analysis and content analysis methods. It proposes a general framework for blockchain smart contract research applied to this industry. The paper provides a qualitative and quantitative analysis of blockchain smart contracts in the agri-food industry, clarifying the research status, challenges, and development trends. It contributes to the body of knowledge and promotes the strategic adoption and use of blockchain smart contracts in the agri-food industry.

COMPUTERS AND ELECTRONICS IN AGRICULTURE (2023)

Article Food Science & Technology

Research on the Construction of Grain Food Multi-Chain Blockchain Based on Zero-Knowledge Proof

Boyang Zhang, Jiping Xu, Xiaoyi Wang, Zhiyao Zhao, Shichao Chen, Xin Zhang

Summary: As the main food source of the world's population, grain quality safety is crucial for human beings. To strengthen the information processing and coordination of the grain food supply chain, an information management model based on blockchain multi-chain technology is studied.
Article Remote Sensing

A Path-Planning Method Considering Environmental Disturbance Based on VPF-RRT*

Zhihao Chen, Jiabin Yu, Zhiyao Zhao, Xiaoyi Wang, Yang Chen

Summary: This paper proposes a path-planning method considering environmental disturbance based on virtual potential field RRT* (VPF-RRT*). It introduces a VPF-RRT* algorithm for planning the path and an anti-environmental disturbance method based on a deep recurrent neural networks PI (DRNN-PI) controller. Comparative simulation experiments demonstrate the effectiveness of the proposed algorithm in achieving a shorter planning path and maintaining navigation along the planned path in the presence of environmental disturbances.

DRONES (2023)

Proceedings Paper Engineering, Electrical & Electronic

A CMOS COMPATIBLE MICRO PIRANI GAUGE WITH STRUCTURE OPTIMIZATION FOR PERFORMANCE ENHANCEMENT

Rui Jiao, Gai Yang, Ruoqin Wang, Yue Tang, Zhongyi Liu, Huikai Xie, Hongyu Yu, Xiaoyi Wang

Summary: In this paper, a CMOS compatible micro-Pirani gauge was developed using a CMOS compatible fabrication process. The study revealed that a tiny fluid conduction gap can enhance sensitivity and improve the dynamic measurement range, especially in high-pressure regions. CFD simulation and experimental results confirmed the higher sensitivity and larger measurement range achieved by the T-type device compared to the L-type device, with good stability.

2023 IEEE 36TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, MEMS (2023)

Proceedings Paper Engineering, Electrical & Electronic

A PERFORMANCE ENHANCED THERMAL FLOW SENSOR WITH NOVEL DUAL-HEATER STRUCTURE USING CMOS COMPATIBLE FABRICATION PROCESS

Zhongyi Liu, Ruoqin Wang, Gai Yang, Xinyuan Zhang, Rui Jiao, Xuejiao Li, Jiali Qi, Hongyu Yu, Huikai Xie, Xiaoyi Wang

Summary: A CMOS compatible calorimetric flow sensor with a dual-heater (DH) structure is proposed, which enhances the sensitivity and performance of the device compared to the traditional single-heater (SH) design. Experimental results show that the DH sensor achieves a high sensitivity of 403.25 mV/(m/s)/W in the parallel-heater (PH) mode, which is over 4 times that of the half-bridge (HB) mode. The DH flow sensor under its PH mode also provides an ultra large measurement range and fast response time.

2023 IEEE 36TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, MEMS (2023)

Article Engineering, Electrical & Electronic

A Sub-5mW Monolithic CMOS-MEMS Thermal Flow Sensing SoC With ± 6 m/s Linear Range

Wei Xu, Zhijuan Li, Zetao Fang, Bo Wang, Linze Hong, Gai Yang, Su-Ting Han, Xiaojin Zhao, Xiaoyi Wang

Summary: This article presents a CMOS-MEMS monolithic integrated thermal flow sensor system with high accuracy, low power consumption, and low noise. The sensor has high sensitivity, wide measurement range, and good linearity, which has been experimentally verified to be feasible.

IEEE JOURNAL OF SOLID-STATE CIRCUITS (2023)

Proceedings Paper Engineering, Electrical & Electronic

A FLEXIBLE THERMAL FLOW SENSOR WITH QUADRUPLE HEATERS AND SUSPENDED STRUCTURE FOR PERFORMANCE ENHANCEMENT

Xiaoyi Wang, Xingru Chen, Yang Deng, Yik Kin Cheung, Peng Jiang, Wei Xu, Hongyu Yu

Summary: A novel flexible flow sensor with quadruple heaters and a suspended structure is reported for the first time, exhibiting excellent performance. The sensor, integrated with platinum thermal resistors and an ultra-thin Parylene C film, achieves significantly higher sensitivity and better symmetrically bidirectional sensing function, high repeatability, and stability compared to traditional designs. With its excellent performance and flexibility, this sensor is considered a promising candidate for wearable respiration monitoring devices.

2022 IEEE 35TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS CONFERENCE (MEMS) (2022)

Article Biophysics

An integrated centrifugal microfluidic strategy for point-of-care complete blood counting

Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari

Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Immunity testing against COVID-19 from blood by an IoT-enabled and AI-controlled multiplexed microfluidic platform

Nabil H. Bhuiyan, Joon S. Shim

Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Influence of shear stress on electroactive biofilm characteristics and performance in microbial fuel cells

Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour

Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Cheap and portable paper chip with terrific oxidase-like activity and SERS enhancement performance for SERS-colorimetric bimodal detection of intracellular glutathione

Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li

Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Tracking the EMT-like phenotype switching during targeted therapy in melanoma by analyzing extracellular vesicle phenotypes

Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau

Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.

BIOSENSORS & BIOELECTRONICS (2024)

Review Biophysics

Protease detection in the biosensor era: A review

Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov

Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Controllable self-assembled DNA nanomachine enable homogeneous rapid electrochemical one-pot assay of lung cancer circulating tumor cells

Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin

Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Therapeutic drug monitoring mediated by the cooperative chemical and electromagnetic effects of Ti3C2TX modified with Ag nanocubes

Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang

Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

CRISPR-Cas12a powered hybrid nanoparticle for extracellular vesicle aggregation and in-situ microRNA detection

Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu

Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Facile synthesis of dual-ligand europium-metal organic gels for ratiometric electrochemiluminescence detecting I27L gene

Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li

Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

The use of biological fluids in microfluidic paper-based analytical devices (μPADs): Recent advances, challenges and future perspectives

Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho

Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Development of a peptide microarray-based metal-enhanced fluorescence assay for ultrasensitive detection of multiple matrix metalloproteinase activities by using a gold nanorod-polymer substrate

Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang

Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Plasmonic digital PCR for discriminative detection of SARS-CoV-2 variants

Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon

Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

A wearable and flexible lactic-acid/O2 biofuel cell with an enhanced air-breathing biocathode

Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu

Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Gas-responsive two-dimensional metal-organic framework composites for trace visualization of volatile organic compounds

Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang

Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.

BIOSENSORS & BIOELECTRONICS (2024)