Article
Chemistry, Multidisciplinary
David S. Kinnamon, Jacob T. Heggestad, Jason Liu, Thu Nguyen, Vo Ly, Angus M. Hucknall, Cassio M. Fontes, Rhett J. Britton, Jian-Piao Cai, Jasper Fuk-Woo Chan, Kwok-Yung Yuen, Thuy Le, Ashutosh Chilkoti
Summary: We developed a self-contained immunoassay platform that can be used in field settings with varied temperature, humidity, and blood samples. The platform has high sensitivity and accuracy in detecting analyte concentration, and a portable detection device. We also improved the microfluidic cassette for better usability and faster results. By using this platform, we successfully conducted a rapid diagnostic test for talaromycosis infection in patients, showing comparable sensitivity and specificity to laboratory tests.
Article
Medicine, General & Internal
Bin-Feng Yin, Xin-Hua Wan, Ming-Zhu Yang, Chang-Cheng Qian, A. S. M. Muhtasim Fuad Sohan
Summary: This study developed a wave-shaped microfluidic chip assisted multiplexed detection platform, which enables rapid and sensitive detection of multiple biomarkers including CRP, PCT, and IL-6. The detection process is simple and shows good selectivity and repeatability, making it suitable for point-of-care testing.
MILITARY MEDICAL RESEARCH
(2022)
Review
Biotechnology & Applied Microbiology
Kaimin Wu, Xuliang He, Jinglei Wang, Ting Pan, Ran He, Feizhi Kong, Zhenmin Cao, Feiye Ju, Zhao Huang, Libo Nie
Summary: Microfluidic chip technology is a technology platform that integrates basic operation units such as processing, separation, reaction, and detection into a microchannel chip to achieve low consumption, fast and efficient sample analysis. It has the characteristics of small sample and reagent volume, fast analysis, low cost, automation, portability, high throughput, and good compatibility with other techniques. The future development of microfluidic chips lies in point-of-care testing and high throughput equipment, but there are still challenges in the design and integration of microfluidic chips, as well as the analysis of actual samples.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Fangfang Liu, Jingjing Zhou, Xiangxiang Luo, Yu Liu, Chunrong Huang, Xiaowei He, Yu Wang
Summary: A point-of-care chemiluminescent immunoassay was developed for PGI testing in grassroots communities, improving the early diagnosis rate of gastric cancer. This method is fast, highly sensitive, specific, suitable for high-throughput analysis, and remains unaffected after storage for 7 days.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Henry Ordutowski, Cesar Parra-Cabrera, Clement Achille, Ruben Dochy, Francesco Dal Dosso, Jeroen Lammertyn, Rob Ameloot, Dragana Spasic
Summary: Although efficient diagnostic testing often relies on centralized laboratories, rapid diagnostics at the point-of-care (POC) are increasingly investigated to provide an affordable and easy-to-use alternative that requires minimal external equipment. 3D printing is a promising technology to enable the single-step fabrication of POC diagnostic devices. In this study, the researchers aimed to improve the time-to-result and detection limit of the 3D-printed microfluidic device used for enzyme-linked immunosorbent assay (ELISA) by designing a second-generation device and optimizing the IgE ELISA.
APPLIED MATERIALS TODAY
(2023)
Article
Chemistry, Analytical
Nan Li, Minjie Shen, Youchun Xu
Summary: A portable microfluidic system was established for point-of-care detection of multiple protein biomarkers, combining a protein microarray and a microfluidic cassette. The system automatically conducted immunoassays for alpha-fetoprotein, carcinoembryonic antigen, and carcinoma antigen 125 within 40 minutes, with good correlations to results from a commercial instrument. This versatile platform shows great potential for rapid and sensitive multiplexed detection of biomarkers in POC diagnostics.
Article
Chemistry, Analytical
Suchanat Boonkaew, Katarzyna Szot-Karpinska, Joanna Niedziolka-Jonsson, Barbara Palys, Martin Jonsson-Niedziolka
Summary: This study presents a novel sequential flow-through microfluidic device for assessing the risk of cardiovascular disease using C-reactive protein as the model analyte. The device features dual flow behaviors and requires only a single buffer loading, resulting in a total analysis time of 15 minutes. The integration of the P3-CRP peptide as a new bioreceptor enhances the performance of the device.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Hilal Torul, Zeynep Caglayan Arslan, Tugba Tezcan, Elif Calik Kayis, Merve Calimci, Aysen Gumustas, Ender Yildirim, Haluk Kulah, Ugur Tamer
Summary: Microfluidics allows for the integration of various laboratory protocols on a single system, offering advantages such as efficient transportation, reduced sample volumes, fast analysis time, and improved sensitivity. The combination of immunoassays and microfluidic technology has great potential as a biosensor system for blood samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Aihua Lyu, Tengchuan Jin, Shanshan Wang, Xiaoxue Huang, Weihong Zeng, Rui Yang, Hua Cui
Summary: An automatic label-free chemiluminescence immunoassay (CLIA) for rapid SARS-CoV-2 nucleocapsid protein (NP) detection with high sensitivity and throughput has been developed. It integrates dualfunctionalized chemiluminescent magnetic beads, an automatic chemiluminescence analyzer, and a novel detection strategy, showing great potential for practical use in detecting SARS-CoV-2 NP.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Nanoscience & Nanotechnology
Sura A. Muhsin, Amjed Abdullah, Estela Kobashigawa, Muthana Al-Amidie, Sherri Russell, Michael Z. Zhang, Shuping Zhang, Mahmoud Almasri
Summary: Chronic wasting disease (CWD) is a fatal neurologic disease in cervids caused by misfolded prion protein. The current diagnosis relies on ELISA screening and subsequent immunohistochemistry (IHC) confirmation, but has limitations in sensitivity and specificity. We have developed a microfluidic microelectromechanical system (MEMS) biosensor that offers accurate, rapid, and low-cost detection of CWD pathologic prions in retropharyngeal lymph nodes (RLNs), with higher sensitivity than ELISA.
MICROSYSTEMS & NANOENGINEERING
(2023)
Review
Medicine, Research & Experimental
Trey W. Pittman, Daniel Balazs Decsi, Chamindie Punyadeera, Charles S. Henry
Summary: There has been a growing interest in using microfluidic devices for saliva analysis in recent years. This approach has the potential to provide more sensitive and selective analysis by collecting large quantities of saliva samples. The aim of this review is to provide an update on the use of microfluidic devices for salivary biomarker analysis.
Article
Chemistry, Analytical
Chunxiao Hu, Valerio F. Annese, Michael P. Barrett, David R. S. Cumming
Summary: Venous thromboembolism (VTE) is a blood clot that originates in a vein. It is most likely to occur after major surgery, major injury, heart failure, cancer, or infectious diseases such as COVID-19. Prompt treatment is necessary to prevent the restriction or blockage of blood flow and oxygen, which can lead to tissue or organ damage. Imaging technologies or rapid measurements of thrombin generation (TG) and D-dimer are used for diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wanghong He, Minli You, Zedong Li, Lei Cao, Feng Xu, Fei Li, Ang Li
Summary: The study developed a lateral flow immunoassay strip platform for simultaneous detection of three periodontitis biomarkers using upconversion nanoparticles, achieving successful results in clinical samples. The platform demonstrated high sensitivity and detection specificity, as well as rapid detection capabilities.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Binfeng Yin, Xinhua Wan, Wenkai Yue, Teng Zhou, Liuyong Shi, Songbai Wang, Xiaodong Lin
Summary: Abusive use of 13-agonists can harm human health and food safety. Conventional assays cannot meet the demand for automated, high throughput, and rapid quantitative analysis of multiple 13-agonists in real samples. A portable automated chip system (PACS) was developed in this study to provide simultaneous point-of-care testing of multiple 13-agonists in the field, simplifying complex manual methods, shortening assay times, and improving sensitivity. The PACS enables sensitive and rapid detection of multiple 13-agonists in farms or markets lacking advanced laboratory facilities.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Shih-Mo Yang, Shuangsong Lv, Wenjun Zhang, Yubao Cui
Summary: Early diagnosis of infectious diseases is crucial for increasing recovery rates and preventing disease spread. However, in areas with limited medical facilities, timely detection of diseases is challenging. Microfluidic point-of-care (POC) devices offer a rapid and cost-effective solution for disease detection. This review provides an overview of microfluidic POC devices and their applications, emphasizing their potential in underdeveloped regions to mitigate the impact of diseases and improve quality of life. Discussions include current applications in infectious diseases, cardiovascular diseases, tumors, and chronic diseases, as well as future possibilities in wearable devices and telemedicine. The review concludes by analyzing the current state of the microfluidic field and proposing recommendations to establish standards for these devices.
Article
Biochemical Research Methods
Jiahui Guo, Yizhi Cui, Ziqi Yan, Yanzhang Luo, Wanling Zhang, Suyuan Deng, Shengquan Tang, Gong Zhang, Qing-Yu He, Tong Wang
JOURNAL OF PROTEOME RESEARCH
(2016)
Article
Immunology
Shengquan Tang, Suyuan Deng, Jiahui Guo, Xing Chen, Wanling Zhang, Yizhi Cui, Yanzhang Luo, Ziqi Yan, Qing-Yu He, Shan Shen, Tong Wang
JOURNAL OF IMMUNOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Shan Shen, Jiahui Guo, Yanzhang Luo, Wanling Zhang, Yizhi Cui, Qing Wang, Zhiguang Zhang, Tong Wang
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2014)
Article
Multidisciplinary Sciences
Yanzhang Luo, Tin Seak Mok, Xiuxian Lin, Wanling Zhang, Yizhi Cui, Jiahui Guo, Xing Chen, Tao Zhang, Tong Wang
SCIENTIFIC REPORTS
(2017)
Article
Engineering, Environmental
Yanzhang Luo, Shiqing Sun, Hui Zhang
WATER ENVIRONMENT RESEARCH
(2019)
Article
Oncology
Dongxiu Rong, Xiuxian Lin, Yanzhang Luo, Tin Seak Mok, Qing Wang, Haiyan Wang, Tao Zhang
TRANSLATIONAL CANCER RESEARCH
(2020)
Article
Biochemical Research Methods
Enqi Huang, Yu Wang, Na Yang, Bowen Shu, Guohao Zhang, Dayu Liu
Summary: The Onestart system, utilizing a microfluidic chip, enables rapid and accurate detection of respiratory tract infection pathogens, with the entire analysis process completed within 1.5 hours. The system shows excellent specificity and stability in detecting pathogens.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Bowen Shu, Ling Lin, Bin Wu, Enqi Huang, Yu Wang, Zhujun Li, Haoyan He, Xiuxia Lei, Banglao Xu, Dayu Liu
Summary: An ultraportable RNA amplification testing device is introduced for rapid screening of infectious pathogens, utilizing 3D-printed structural parts and off-the-shelf mechanical/electronic components. By preloading necessary reagents in a multi-channel droplet array chip, the device enables seamless integration of bacteria lysis, RNA extraction, and amplification, while providing visual readout and geometrically-multiplexed quantitative detection through an integrated wireless video camera.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Dongguo Lin, Xiao Chen, Yang Liu, Zhun Lin, Yanzhang Luo, Mingpeng Fu, Na Yang, Dayu Liu, Jie Cao
Summary: The microfluidic single-cell culture approach allows for expansion and recovery of colorectal cancer stem cells, and identification of multiple CSC-specific targets through transcriptomic analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Biomaterials
Yang Liu, Xiao Chen, Jueming Chen, Yanzhang Luo, Zihe Chen, Dongguo Lin, Jianye Zhang, Dayu Liu
Summary: A gel-free single-cell culture array on a microfluidic chip was developed to efficiently obtain tumor stem cells (TSCs) with multi-lineage differentiation potential.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Dongyang Cai, Yu Wang, Jingjing Zou, Zhujun Li, Enqi Huang, Xiuyun Ouyang, Zhiquan Que, Yanzhang Luo, Zhenhua Chen, Yanqing Jiang, Guohao Zhang, Hongkai Wu, Dayu Liu
Summary: To overcome the limitation in multiplexing capacity of current digital nucleic acid analysis (DNAA) methods, a droplet encoding-pairing enabled DNAA multiplexing strategy is developed. This strategy utilizes unique tricolor combinations to index individual primer droplets, and combines microfluidic chip and machine learning algorithm for droplet trapping and analysis. By incorporating amplification signals with droplet encoding information, simultaneous quantitative detection of multiple targets is achieved.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
