Article
Chemistry, Analytical
Kaifei Jin, Xiyue Xie, Jing Zhu, Zhenqiang Wang, Yuxin Xing, Lu Wang, Kaiyong Cai, Jixi Zhang
Summary: A homogeneous and label-free miRNA sensing system was developed using magnetically separable spherical nucleic acids (SNA) and signal amplification in PEC detection. The system achieved highly repeatable and anti-interference analysis of miR-21 in complex physiological samples, with a low limit of detection. It was successfully applied for sensitive profiling of miR-21 in tumor cells and tumor-bearing mice plasma.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Hao Jiang, Yuan Li, Xuefei Lv, Yulin Deng, Xiaoqiong Li
Summary: Nucleic acid amplification techniques, such as polymerase chain reaction (PCR) and isothermal amplification, are important for nucleic acid detection. However, PCR is limited by temperature control and isothermal amplification has its own limitations. Efforts to integrate enzymes and amplification techniques have the potential to overcome these limitations. This review provides a comprehensive summary of cascade amplification, including design fundamentals, signal generation, evolution, applications, challenges, and trends.
Article
Biophysics
Jingjing Zhang, Chunyuan Song, Yunfeng Zhu, Hongyu Gan, Xinyue Fang, Qian Peng, Jingrong Xiong, Chen Dong, Caiqin Han, Lianhui Wang
Summary: In this study, a novel one-pot cascade signal amplification strategy integrating the CRISPR/Cas13a system and branched hybridization chain reaction (bHCR) was proposed for highly sensitive and specific SERS assay of disease-related nucleic acids. The method showed low detection limit, good linear calibration curve, and demonstrated good practicality in clinical samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Kemei Jiang, Yanan Wu, Juan Chen, Mingqing Shi, Hong-Min Meng, Zhaohui Li
Summary: A molecular recognition triggered aptazyme cascade strategy was developed for ultrasensitive detection of cancer exosomes in clinical serum samples. This strategy showed high sensitivity and specificity, making it suitable for cancer diagnosis and biological studies.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Jasper W. Dittmar, Michelle H. Teplensky, Michael Evangelopoulos, Lei Qin, Bin Zhang, Chad A. Mirkin
Summary: The stability of the liposome core is crucial for the effectiveness of liposome-based drugs. This study explores the impact of anchor chemistry on immunotherapeutic function and demonstrates that a stable anchor can enhance immune stimulation and improve therapeutic efficacy.
Article
Chemistry, Multidisciplinary
Tianyu Du, Zewen Shi, Zhaojian Qin, Yiwei Hu, Yabin Zhu, Hui Jiang, Xuemei Wang
Summary: This study proposes an in situ targeted therapy strategy using a photothermally triggered nanoplatform. The nanoplatform, consisting of spherical nucleic acids, black phosphorus nanosheets, and zinc porphyrin metal-organic framework nanosheets, can disassemble during photothermal therapy, allowing for photothermally triggered photodynamic therapy. The enhanced singlet oxygen generation decreases heat shock proteins and improves the therapeutic effect. The superstructure shows potential as a synergistic platform for advanced cancer theranostics.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Biophysics
Zhenqiang Ning, Mengyuan Chen, Guoqiu Wu, Yuanjian Zhang, Yanfei Shen
Summary: Nucleic acids play a significant role in ECL biosensing, offering versatile selective molecular recognition and programmable self-assembly for robust nanostructure construction. ECL biosensing based on nucleic acids has seen rapid growth due to their unique structures and physiochemical properties, with applications as molecular recognition elements, signal amplification units, and sensing interface schemes for future developments.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Marjon Zamani, James M. Robson, Andy Fan, Michael S. Bono, Ariel L. Furst, Catherine M. Klapperich
Summary: Sexually transmitted infections, such as HIV and HPV, have a disproportionate impact on low-resource settings. Early diagnosis is crucial for managing HIV and preventing HPV-induced cervical cancer. A novel electrochemical platform combined with loop-mediated isothermal amplification and CRISPR-based detection shows promise for rapidly and cost-effectively detecting viral infections.
ACS CENTRAL SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Yuanyuan Chen, Peng Gao, Wei Pan, Mingwan Shi, Shujie Liu, Na Li, Bo Tang
Summary: The research developed an X-ray-induced phenotypic transformation strategy through macrophage engineering for safe and efficient tumor immunotherapy. The engineered macrophages demonstrated high tumor recognition and X-ray-induced polarization effects, significantly inhibiting tumor growth in mice even with a reduced radiation dose compared to natural macrophages. The X-PT strategy opens a new avenue for clinical immune cell-based therapy.
Article
Nanoscience & Nanotechnology
Fangfei Yin, Haipei Zhao, Shasha Lu, Juwen Shen, Min Li, Xiuhai Mao, Fan Li, Jiye Shi, Jiang Li, Baijun Dong, Wei Xue, Xiaolei Zuo, Xiurong Yang, Chunhai Fan
Summary: The article introduces a DNA-encoded molecular classifier that can classify multidimensional molecular clinical data and has been tested in prostate cancer patients.
NATURE NANOTECHNOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Yongxi Zhao, Xiaolei Zuo, Qian Li, Feng Chen, Yan-Ru Chen, Jinqi Deng, Da Han, Changlong Hao, Fujian Huang, Yanyi Huang, Guoliang Ke, Hua Kuang, Fan Li, Jiang Li, Min Li, Na Li, Zhenyu Lin, Dingbin Liu, Juewen Liu, Libing Liu, Xiaoguo Liu, Chunhua Lu, Fang Luo, Xiuhai Mao, Jiashu Sun, Bo Tang, Fei Wang, Jianbin Wang, Lihua Wang, Shu Wang, Lingling Wu, Zai-Sheng Wu, Fan Xia, Chuanlai Xu, Yang Yang, Bi-Feng Yuan, Quan Yuan, Chao Zhang, Zhi Zhu, Chaoyong Yang, Xiao-Bing Zhang, Huanghao Yang, Weihong Tan, Chunhai Fan
Summary: Nucleic acids are natural biopolymers that play central roles in storing, encoding, transmitting and expressing genetic information. The field of nucleic acids analysis has rapidly advanced with technological breakthroughs, leading to the development of new analysis methods. However, there are also challenges and further research needed in this area.
SCIENCE CHINA-CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Yang Fang, Xueguang Lu, Dali Wang, Jiansong Cai, Yuyan Wang, Peiru Chen, Mengqi Ren, Hao Lu, Jennifer Union, Lei Zhang, Yehui Sun, Fei Jia, Xi Kang, Xuyu Tan, Ke Zhang
Summary: A self-assembled, skin-depigmenting SNA structure was reported in this study, showing a reduction in melanin content in melanoma cells and a potent antimelanogenic effect in an ultraviolet B-induced hyperpigmentation mouse model.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Hongfei He, Yan Zhou, Bin Chen, Yi Zhang, Xiaowu Zhong, Lei Xu, Bin Guo, Chong Yin, Xi Zhou, Qingrong Li, Zhen Huang, Guangcheng Luo, Xiaolan Guo
Summary: A colorimetric isothermal sensing platform (SFMC) with specific signal filtration and magnification has been developed for ultrasensitive detection of DNA and RNA. This platform can detect target DNA as low as 5 copies per reaction and target RNA as low as 10 copies per reaction by naked eyes. It has also been successfully used for the detection of African swine fever virus (ASFV) and SARS-CoV-2.
Article
Biophysics
Huinan Chen, Yuanyuan Li, Yuchen Song, Fujing Liu, Dongmei Deng, Xiaoli Zhu, Haibo He, Xiaoxia Yan, Liqiang Luo
Summary: In this research, an innovative sandwich-type electrochemical immunosensor is designed for the ultrasensitive determination of tumor biomarker AFP using spherical nucleic acids-templated silver nanoclusters (AgNCs) sensing platform. The immunosensor exhibits broad linearity range and low detection limit, providing a novel method for sensitive clinical diagnosis of tumor markers in human hepatocellular carcinoma.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Yue Zhao, Xiaoxing Fang, Min Bai, Jin Zhang, Huahang Yu, Feng Chen, Yongxi Zhao
Summary: In this study, a microfluidic surface-enhanced Raman scattering (SERS) sensor was developed for online detection of exosomal miRNA. The sensor achieved sensitive detection of abnormal exosomal miRNA using a rolling circle amplification (RCA) and tyramine signal amplification (TSA) strategy, showing potential practical applications.
CHINESE CHEMICAL LETTERS
(2022)
Article
Biotechnology & Applied Microbiology
Mingde Huang, Jiakai Hou, Yunfei Wang, Min Xie, Chenchen Wei, Fengqi Nie, Zhaoxia Wang, Ming Sung
Article
Oncology
Xin Chen, Zhenyao Chen, Shanxun Yu, Fengqi Nie, Shuai Yan, Pei Ma, Qinnan Chen, Chenchen Wei, Hangjiang Fu, Tianwei Xu, Shengnan Ren, Ming Sun, Zhaoxia Wang
CLINICAL CANCER RESEARCH
(2018)
Review
Biotechnology & Applied Microbiology
Tianwei Xu, Lihua Jiang, Zhaoxia Wang
ONCOTARGETS AND THERAPY
(2019)
Article
Oncology
Shanxun Yu, Daolu Yang, Yunyao Ye, Pei Liu, Zhenyao Chen, Tianyao Lei, Jiaze Pu, Longfa Liu, Zhaoxia Wang
Article
Medicine, Research & Experimental
Tianwei Xu, Shuai Yan, Lihua Jiang, Shanxun Yu, Tianyao Lei, Daolu Yang, Binbin Lu, Chenchen Wei, Erbao Zhang, Zhaoxia Wang
MOLECULAR THERAPY-NUCLEIC ACIDS
(2019)
Review
Medical Laboratory Technology
Zhenyao Chen, Tianyao Lei, Xin Chen, Jingyao Gu, Jiali Huang, Binbin Lu, Zhaoxia Wang
CLINICA CHIMICA ACTA
(2020)
Article
Oncology
Zhenyao Chen, Xin Chen, Binbin Lu, Yu Gu, Qinnan Chen, Tianyao Lei, Fengqi Nie, Jingyao Gu, Jiali Huang, Chenchen Wei, Ming Sun, Zhaoxia Wang
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2020)
Review
Medicine, Research & Experimental
Jiali Huang, Zhenyao Chen, Xin Chen, Jun Chen, Zhixiang Cheng, Zhaoxia Wang
Summary: m(6)A is a modification of eukaryotic RNA that is dynamically regulated during cell metabolism and embryo development, mainly involved in various aspects of RNA metabolism, and closely related to the occurrence and development of cancer. Methyltransferases, as key elements in the dynamic regulation of m(6)A, play a crucial role in these processes.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Review
Biotechnology & Applied Microbiology
Mengwei Wang, Tianwei Xu, Wenyan Feng, Junxia Liu, Zhaoxia Wang
Summary: Long noncoding RNAs (lncRNAs) are RNA molecules longer than 200 nucleotides that do not encode proteins. Dysregulation of lncRNAs and their involvement in key cancer-related signaling pathways, such as the Hippo signaling pathway, play a crucial role in cancer development and progression. Understanding the role of lncRNAs in modulating the Hippo signaling pathway provides valuable insights for the development of novel strategies for early detection and treatment of cancer.
ONCOTARGETS AND THERAPY
(2021)
Review
Oncology
Li Ma, Jin Gao, Niu Zhang, Jiawei Wang, Tianwei Xu, Tianyao Lei, Xiaoteng Zou, Chenchen Wei, Zhaoxia Wang
Summary: This review summarizes the studies on SNHG17 in recent years, highlighting its high expression in various tumors and its potential impact on the biological function and clinical applications of tumor cells.
CANCER CELL INTERNATIONAL
(2022)
Review
Medicine, Research & Experimental
Junxia Liu, Qinqiu Zhang, Daolu Yang, Fei Xie, Zhaoxia Wang
Summary: Long non-coding RNAs (lncRNAs) play a crucial role in tumor angiogenesis and resistance to anti-angiogenic therapy. Understanding their functions and potential clinical applications is important for improving tumor treatment.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Retraction
Biotechnology & Applied Microbiology
[Anonymous]
ONCOTARGETS AND THERAPY
(2023)
Article
Oncology
Jin Gao, Yadong Li, Xiaoteng Zou, Tianyao Lei, Tianwei Xu, Yijiang Chen, Zhaoxia Wang
Summary: HEY1 was found to regulate cisplatin resistance in lung adenocarcinoma by modulating the epithelial-mesenchymal transition (EMT) process. High expression of HEY1 was associated with poor prognosis after cisplatin therapy.
Article
Chemistry, Multidisciplinary
Zhaoxia Wang, Xinming Li, Zhixing Shao, ZhengFang, Yueping Zhai
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)