期刊
BIOSENSORS & BIOELECTRONICS
卷 193, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113540
关键词
Carbon-dots; Magnetoplasmonic-Ag-nanocubes; Dual-modality; Fluorescence-SERS; Immunocomplex; Norovirus
类别
资金
- Japan Society for the Promotion of Science (JSPS) [19F19348]
- Japan Agency for Medical Research and Development [20hm0102080h0001]
- Grants-in-Aid for Scientific Research [19F19348] Funding Source: KAKEN
Synergistic dual-mode optical platforms with novel dual-modality fluorescence and surface-enhanced Raman scattering techniques have been integrated into a biosensor for the rapid and ultrasensitive detection of norovirus. The biosensor shows a wide detection range and excellent detection limit, as well as the ability to track clinical norovirus in fecal specimen effectively.
Synergistic dual-mode optical platforms are up-and-coming detection tools in the diagnosis and management of infectious diseases. Here, novel dual-modality fluorescence (FL) and surface-enhanced Raman scattering (SERS) techniques have been integrated into a single probe for the rapid and ultrasensitive detection of norovirus (NoV). The developed FL-SER-based biosensor relies on the dual-signal enhancements of newly synthesized sulfur-doped agar-derived carbon dots (S-agCDs). The antigen-antibody immunoreaction results in forming a core-satellite immunocomplex between anti-NoV antibody-conjugated S-agCDs and polydopamine-functionalized magnetic silver nanocubes [poly (dop)-MNPs-Ag NCs]. By deploying an immunomagnetic enrichment protocol and performing the SERS modality on a single-layer graphene substrate, norovirus-like particles (NoV-LPs) were detected across a wide range of 1 fg mL(-1) - 10 ng mL(-1) with an excellent limit of detection of 0.1 fg mL(-1). The combined advantage of the dual-signaling properties of the biosensor was demonstrated using FL confocal imaging for hotspots tracking prior to SERS detection of clinical NoV in fecal specimen down to -10 RNA copies mL(-1). The proposed dual-modality biosensor's performance increases the prospect of a rapid and low-cost sensitive NoV detection and surveillance option for public health.Y
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