Article
Chemistry, Analytical
Shiying Zhou, Jiangbo Dong, Liyuan Deng, Zhen Huang, Peng Lu, Guixue Wang, Mei Yang, Danqun Huo, Changjun Hou
Summary: In this study, a CRISPR/Cas12a-driven fluorescent and electrochemical dual-mode biosensor was developed for detecting the mutation status of epidermal growth factor receptor exon 19. The biosensor exhibited high detection accuracy and sensitivity, and could discriminate wild-type and mutant genes from various cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biophysics
Fan Li, Qinghua Ye, Moutong Chen, Baoqing Zhou, Jumei Zhang, Rui Pang, Liang Xue, Juan Wang, Haiyan Zeng, Shi Wu, Youxiong Zhang, Yu Ding, Qingping Wu
Summary: The study introduces the trans-cleavage activity of CRISPR/Cas12a into an electrochemical biosensor (ECRISPR) combined with recombinase-assisted amplification (RAA) to develop a cost-effective, specific, and ultrasensitive method for Listeria monocytogenes detection. The RAA-based E-CRISPR sensor can detect as low as 0.68 aM of genomic DNA and 26 cfu/mL of L. monocytogenes in pure cultures, demonstrating rapid and ultrasensitive detection capabilities in spiked and natural samples. This system shows no cross-reactivity with other non-target bacteria, making it a simple, highly sensitive, and accurate platform for L. monocytogenes detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Feng Qiu, Xiaoyan Gan, Jianglong Yao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a sensitive detection method for nuclear factor-kappa B p50 (NF-KB p50) was proposed by integrating hybridization chain reaction (HCR)-activated Cas12a enzyme with bio-responsive DNA hydrogels. The method achieved highly enhanced electrochemical signals for detecting target molecules at a low detection limit and successfully detected NF-KB p50 in lysate of HeLa cells.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Mengyan Li, Nian Luo, Xiaofei Liao, Li Zou
Summary: We developed a dual signal amplification strategy for sensitive detection of circulating tumor DNA (ctDNA) based on hybridization chain reaction (HCR) and proximity hybridization-regulated CRISPR/Cas12a. This strategy exhibits high sensitivity toward ctDNA with a low detection limit of 5.43 fM, and it has been successfully applied for ctDNA detection in serum samples, showing great potential in the clinical diagnosis of cancer.
Article
Chemistry, Analytical
Qinli Pu, Yuanyuan Ye, Juan Hu, Cong Xie, Xi Zhou, Hongyan Yu, Fangli Liao, Song Jiang, Linshan Jiang, Guoming Xie, Weixian Chen
Summary: This work proposes a new strategy based on XNA probe and CRISPR/Cas12a signal amplification for the sensitive detection of site-specific m6A modifications. The specificity and sensitivity of m6A detection are greatly improved by the combined use of XNA probe and crRNA. The application of CRISPR/Cas12a to different biosensing systems enhances the accuracy of m6A modification detection.
Article
Chemistry, Analytical
Lianyu Lu, Huimin Zhang, Fanghe Lin, Leiji Zhou, Zhi Zhu, Chaoyong Yang
Summary: The development of new diagnostic platforms for sensitive and rapid detection of bacterial pathogens is significant for improving precision medication for infectious disease and food safety control. The DMF-Cas12a method, which integrates the CRISPR/Cas12a system with a digital microfluidic chip, enables rapid, sensitive, and automated analysis of bacterial pathogens. The platform shows good potential in automated and highly sensitive pathogen detection for a wide variety of biological applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Junyi Zhang, Jie Xiang, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: We have developed a highly sensitive fluorescent thrombin biomarker sensing method by combining the DNA walker and CRISPR/Cas12a system. This method utilizes the DNA moving arms to localize on AuNP tracks and generate CRISPR/Cas12a-responsive dsDNAs, which restores the fluorescence by pushing the dye away from AuNPs. The method has a detection limit of 29.5 fM and has been verified for selectively detecting thrombin against interference proteins and in diluted serums, making it a promising approach for disease diagnosis.
Article
Biochemistry & Molecular Biology
Na Liu, Ran Liu, Jingjing Zhang
Summary: This article introduces a CRISPR-Cas12a-based electrochemical sensor for quantitative detection of SARS-CoV-2 nucleocapsid antigen. The sensor combines aptamer-specific recognition with CRISPR-Cas12a-mediated signal amplification, demonstrating excellent analytical performance.
BIOELECTROCHEMISTRY
(2022)
Article
Biochemical Research Methods
Yang Song, Ke Gao, Xiaoying Cai, Wei Cheng, Shijia Ding, Decai Zhang, Shixiong Deng
Summary: A controllable crRNA self-transcription aided dual-amplified CRISPR-Cas12a strategy, named CST-Cas12a, has been developed for highly sensitive and specific biosensing of flap endonuclease 1 (FEN1). This strategy utilizes a branched DNA probe as a hydrolysis substrate for FEN1, and generates double-stranded DNA (dsDNA) containing a T7 promoter and crRNA transcription template. With the help of T7 RNA polymerase, abundant crRNA is produced and assembled with Cas12a to form a Cas12a/crRNA complex. This complex can be activated by a dsDNA trigger and unlocks the fluorophore-quencher reporter cleavage, enabling highly efficient dual signal amplification and near-zero background. Under optimized conditions, the CST-Cas12a method allows highly sensitive biosensing of FEN1 activity with excellent specificity in the presence of other interfering enzymes. Furthermore, this strategy has been successfully applied to FEN1 biosensing in complex biological samples.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Analytical
Luqi Huang, Ning Yuan, Wei Guo, Yunzhe Zhang, Wei Zhang
Summary: A novel electrochemical biosensor based on saltatory rolling circle amplification (SRCA) and CRISPR/Cas12a system was developed for accurate detection of Staphylococcus aureus. The biosensor showed high sensitivity, specificity, and accuracy, providing a potential powerful platform for the detection of S. aureus.
Article
Chemistry, Analytical
Haowei Duan, Yizhou Wang, Shi-Yang Tang, Ting-Hui Xiao, Keisuke Goda, Ming Li
Summary: This study presents a carbon-based E-CRISPR modified with gold nanoparticles and MXene Ti3C2 for stable and sensitive detection of nucleic acids. The developed sensor shows excellent analytical performance and long-term shelf life.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Zhiqing Yang, Zixuan Guo, Haoyu Yuan, Yaping Li, Yuanzhao Hu, Xiu-Qing Li, Yi Wan
Summary: A T7 exonuclease-assisted PAM-free Dual CRISPRCas12a Biosensor (TDCB) was developed for ultrasensitive and highly specific MRSA detection. The biosensor can detect MRSA with high sensitivity and distinguish it from other bacterial species. This technology can be widely used in hospitals, food and feed industry, and fish and dairy farming.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Jie Xu, Zhujun Liu, Zhen Zhang, Tongbo Wu
Summary: The trans-cleavage activity of Cas12a is affected by the length of the fluorescent probe and the reaction buffer. The optimal conditions for Cas12a are a probe length of 15 nucleotides and NEBuffer 4 as the buffer, resulting in a 50-fold improvement in activity and a significant decrease in the detection limit for DNA targets. This method provides a powerful tool for Cas12a trans-cleavage activity applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Cuiying Lin, Qingqing Huang, Mengjian Tian, Fang Luo, Jian Wang, Bin Qiu, Shuofei Yang, Zhenyu Lin
Summary: An electrochemiluminescence (ECL) biosensor with dual signal enhancement was developed to detect DNA adenine methylation methyltransferase (Dam MTase). The biosensor utilized a restriction endonuclease to cleave DNA and activate CRISPR/Cas12a trans-cleavage activity, resulting in enhanced ECL signal. The biosensor exhibited a good linear relationship with Dam MTase concentration and was successfully applied for detection in serum samples.
Article
Engineering, Environmental
Yuqing Zhu, Xiaohe Zheng, Rui Zhu, Hongrui Zhao, Hongli Zhai, Feiyang Qian, Tenghua Zhang, Zihui Xie, Silan Liu, Bowen Jiang, Yan Sheng, Jiaming Hu
Summary: The researchers developed a versatile diagnostic biosensor called SMARTLOCK, which combines DNA tetrahedron, nonlinear HCR and CRISPR-Cas12a system for simultaneous detection of multiple biomarkers. The SMARTLOCK sensor demonstrated high selectivity and sensitivity in detecting miR-141, miR-375, miR21, ATP, cortisol, insulin, and their combinations. This study shows the potential of SMARTLOCK as a highly accurate and cost-effective tool for the screening, diagnosis, and prognosis of various diseases.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Dandan Hu, Tianyu Zhan, Zhiyong Guo, Sui Wang, Yufang Hu
Summary: A novel dual-modality readout biosensing method was developed for the analysis of Cu(II), coenzyme (CoA), and histone acetyltransferase (HAT) activity. The method effectively detects and quantifies these compounds using Ru-MOF as a probe, demonstrating bidirectional detection of Cu(II) and quantification of CoA and HAT p300 activity.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Wenting Wei, Han Lin, Tingting Hao, Xiurong Su, Xiaohua Jiang, Sui Wang, Yufang Hu, Zhiyong Guo
Summary: The dual-mode ECL/SERS immunoassay developed in this study offers a high detection sensitivity through increasing signal tags, electrochemically activating them, and introducing AuNRs for signal enhancement. The linear range and limit of quantification for ECL were 1-10^8 CFU/mL and 1 CFU/mL, while those for SERS were 10^2-10^8 CFU/mL and 10^2 CFU/mL respectively under optimal conditions.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Fengming Xiao, Huiqian Zhou, Han Lin, Hongze Li, Tinglang Zou, Yangbo Wu, Zhiyong Guo
Summary: A novel fast scan digital circuit for voltammetric analysis with precious ohmic drop compensation has been developed in this work. The circuit enables fast scan cyclic voltammetric (FSCV) analysis for both theoretical electrochemical cells and practical electrochemical systems. The high sensitivity of the circuit allows for ultrasensitive electrochemical detection applications, such as a DNA biosensor for ultrasensitive detection of mercuric ions.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Wenting Wei, Han Lin, Tingting Hao, Sui Wang, Yufang Hu, Zhiyong Guo, Xingyu Luo
Summary: The study developed a sensitive biosensor with triple signal outputs for the detection of pathogenic bacteria, achieving high detection sensitivity and accuracy. It shows great potential in applications related to food safety, water quality, and biological contamination.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Daquan Li, Mei Huang, Ziyu Shi, Liang Huang, Jiening Jin, Chenxing Jiang, Wenbo Yu, Zhiyong Guo, Jing Wang
Summary: Antibiotics abuse poses a threat to human health and ecological environment. A competitive lateral flow immunoassay (CLFIA) platform with flexible readout was developed for the rapid detection of enrofloxacin. The platform utilizes three-dimensionally assembled gold nanoparticles as signal reporters, enabling qualitative screening and quantitative detection of enrofloxacin at low concentrations using the naked eye, optical scanner, or smartphone.
ANALYTICAL CHEMISTRY
(2022)
Article
Electrochemistry
Qi Wang, Tingting Hao, Kaiyue Hu, Lingxia Qin, Xinxin Ren, Zhiyong Guo, Sui Wang, Yufang Hu
Summary: A novel circuit system with autonomous compensation solution ohmic drop is developed to improve the signal generation of traditional electrochemical biosensors. It is successfully applied for the analysis of terminal deoxynucleotide transferase (TdT) and its small molecule inhibitors, as well as mimicking TdT-related signal communication in various logic gates.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Xiaoshuang Chen, Dongyu Zhang, Han Lin, Wenting Wei, Tingting Hao, Yufang Hu, Sui Wang, Zhiyong Guo
Summary: A MXene-catalyzed immunosensor was developed for detecting Cry1Ab protein in GM crops, showing high sensitivity and detection capability. The sensor structure, combined with MXene catalysis, enhances the ECL reaction for improved detection sensitivity. The system demonstrated satisfactory performance in terms of selectivity, stability, repeatability, precision, and accuracy, with successful detection of actual samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Physical
Han Lin, Hideyuki Mitomo, Yusuke Yonamine, Zhiyong Guo, Kuniharu Ijiro
Summary: In this study, a stimulus-responsive system based on chiral molecule-achiral plasmonic nanoparticles was proposed, allowing for precise control of plasmon and its induced chiroptical activities. This design provides an open platform for flexible and rational tailoring of plasmonic cores, chiral molecules, and variable dielectrics, which is important for applications in chemical sensing, chiral nanocatalysis, and optical devices.
CHEMISTRY OF MATERIALS
(2022)
Article
Electrochemistry
Qingqing Zhang, Lingxia Qin, Kaiyue Hu, Xinxin Ren, Zhiyong Guo, Sui Wang, Yufang Hu
Summary: A versatile electrochemical platform based on Cu(II) coordination by pyrophosphatase (PPase)-guided triggered-release system is constructed. The platform is sensitive, selective, stable, and convenient for the quantitative detection of PPase, providing an important reference for PPi/PPase-based biological processes and clinical determinations.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Huiqian Zhou, Han Lin, Qi Wang, Tingting Hao, Yufang Hu, Sui Wang, Zhiyong Guo
Summary: We developed an axis-mode donor-DNA-acceptor electrochemical system to distinguish electron transfer modes in DNA. By embedding the redox probe Ag+ in the DNA strand and controlling the number of Ag+, the distance between Ag+ and the electrode, and the chemical environment, the rotation and bending of the DNA strand were restricted, allowing precise control of the electron transfer mechanism. The results showed that electron transfer in DNA occurs through tunneling rather than hopping.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Huiqian Zhou, Wenbo Guo, Shian Wang, Tingting Hao, Zhaoliang Wang, Yufang Hu, Sui Wang, Jianjun Xie, Xiaohua Jiang, Zhiyong Guo
Summary: An electrochemical aptasensor has been developed for ultrasensitive detection of Staphylococcus aureus (SA) based on stepwise signal amplification. The sensor exhibits good detection performance and wide applicability to other pathogens.
Article
Chemistry, Analytical
Jie Zhang, Huiqian Zhou, Tingting Hao, Yiyao Yang, Qingqing Zhang, Jinyun Li, Meng Ye, Yangbo Wu, Wanlei Gao, Zhiyong Guo
Summary: A Faraday cage-type electrochemiluminescence biosensor was developed for the detection of MCF-7 breast cancer cells. Fe3O4-APTs and GO@PTCA-APTs were synthesized as capture and signal units, respectively. The sensor achieved a significant increase in sensitivity by assembling numerous electrochemiluminescence signal probes and adopting a double aptamer recognition strategy.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Kai Kang, Beibei Wang, Xueping Ji, Yuheng Liu, Wenrui Zhao, Yaqing Du, Zhiyong Guo, Jujie Ren
Summary: This study reported a new type of artificial nanozyme based on Hemin-doped-HKUST-1 for the detection of dopamine. The nanozyme showed excellent electrocatalytic activity and potential value in clinical work, demonstrating a high sensitivity and wide linear range for dopamine detection in serum samples.