Article
Biophysics
Yi Li, Mei-Ling Liu, Wen-Bin Liang, Ying Zhuo, Xiao-Jing He
Summary: This study elaborated a universal approach to quickly activate CRISPR/Cas12a for low-abundance biomarker detection based on the amplification strategy of a target-induced spherical nucleic acid enzyme (SNAzyme) network. The prepared biosensor exhibited satisfying detection performance for the biomarker with a wide linear range and a low detection limit, showing significant potential in clinical diagnoses.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Gui Zhang, Lunkai Li, Yuqin Yang, Li Song, Guangrong Kuang, Yingzi Fu
Summary: We developed a green organic electrochemiluminescencence (ECL) luminophore without an aromatic ring structure but with electron-rich functional groups by in-situ growth of poly (ethylene maleate) (PEM) on nano-silica (NS). The combination of PEM-NSs and exonuclease III-assisted catalytic hairpin assembly amplification strategy allowed for the construction of a highly sensitive platform for biomarkers (microRNA-21) detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yang Yang, Jia-Ling Zhang, Wen-Bin Liang, Jin-Ling Zhang, Xiao-Li Xu, Yong-Jiang Zhang, Ruo Yuan, Dong-Rong Xiao
Summary: A conductive bimetallic MOF nanorod (NiCo-HHTP) with excellent electrical conductivity and ECL performance was synthesized using a doping strategy, which could be utilized for constructing ultrasensitive ECL biosensors.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Zhuoxin Ye, Yibing Liu, Meichen Pan, Xiuli Tao, Yuxuan Chen, Pinyi Ma, Ying Zhuo, Daqian Song
Summary: In this study, low-toxic quaternary AgInZnS quantum dots were synthesized and used as novel anodic electrochemiluminescence (ECL) emitters. These QDs exhibited strong and stable ECL emission, a low excitation potential, and high ECL efficiency. An on-off-on ECL biosensor for detecting microRNA-141 was designed based on a dual isothermal enzyme-free strand displacement reaction, achieving cyclic amplification of the target and ECL signal, as well as a switch function. The ECL biosensor showed a wide linear range and low detection limit, making it a promising tool for rapid and accurate diagnosis of clinical diseases.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Analytical
Hui Sun, Ping Zhou, Bin Su
Summary: Electrochemiluminescence (ECL) is chemiluminescence that is triggered by electrochemical reactions. ECL has become a powerful analytical technique widely used in biosensing and imaging due to its unique excitation mode and low background. Semiconductor quantum dots (QDs), as emerging ECL luminophores, have significant advantages over traditional molecular luminophores in terms of luminescence efficiency and signal modulation ability. Therefore, the development of an efficient ECL system using QDs as luminophores is important for improving the sensitivity and detection flux of ECL biosensors.
Article
Chemistry, Analytical
Zuhao Zhang, Lu Zhang, Shujing Wang, Ruifang Liu, Li Li, Peini Zhao, Chaomin Gao, Fengming Chen, Shenguang Ge, Jinghua Yu
Summary: A CRISPR/Cas12a electrochemiluminescence biosensor was developed for ultrasensitive detection of miRNA-21. The biosensor utilized Tribulus terrestris-like AuCu nanoterrestris and DNA tetrahedral nanostructures, and employed a multiple sites DNA walker for signal amplification, achieving efficient detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yuyi Zhou, Yu Lin, Rongxian Ma, Xuecai Tan, Hu Luo, Kejing Huang
Summary: In recent years, there has been an increasing number of biosensor design strategies that have the potential for practical applications in point-of-care testing (POCT) for various human diseases. The latest research in POCT assays aims to improve sensor precision, cost-effectiveness, and time-consumption. This study presents an emerging electrochemiluminescence biosensor and colorimetric method for the rapid, accurate, and sensitive determination of biomarker microRNA-141. The sensor has the advantages of simplicity, speed, high sensitivity, good selectivity, and a wide linear range, making it a promising method for low-cost and high-sensitivity POCT of microRNAs.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Jingjing Jiang, Xuezhong Du
Summary: The label-free ratiometric electrochemiluminescence (ECL) sensing strategy proposed in this study utilizes G-quadruplex/hemin-regulated ECL emissions of CdS quantum dots and luminol as the coreactant for the sensitive detection of target DNA. The ECL biosensor shows a wide response range for T-DNA detection, low detection limit, and excellent selectivity against mismatched base sequences, providing a reliable and sensitive sensing platform for the detection of targets in the analytical community.
Article
Chemistry, Multidisciplinary
Kai Jiao, Qinglong Yan, Linjie Guo, Zhibei Qu, Shuting Cao, Xiaoliang Chen, Qian Li, Ying Zhu, Jiang Li, Lihua Wang, Chunhai Fan, Fei Wang
Summary: A method for efficiently capturing oncogenic microRNAs in living cells by compartmenting nucleic acids on the surface of poly-adenine-based spherical nucleic acids has been reported. The polyA-SNAs exhibit high cell entry efficiency and improved miRNA capture ability, serving as high-efficacy miRNA sponges for inhibiting tumor growth.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Min Zhou, Yao Yin, Yuyan Shi, Zhaoxin Huang, Yu Shi, Mei Chen, Guoliang Ke, Xiao-Bing Zhang
Summary: A stable and sensitive biosensor for ctDNA in complex biological fluid is developed in this study. By utilizing a spherical nucleic acid reporter and cascade CRISPR/Cas12a amplifier, stability and sensitivity are significantly improved.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biophysics
Liang Tian, Jiyuan Zhang, Yi Zhang, Olayinka Oderinde, Caiyun Li, Lili Duan, Yihong Wang, Jiansheng Cui
Summary: A versatile ratiometric electrochemical biosensing system was constructed for the detection of miRNAs, using a combination of target triggered bipedal DNAzyme walker cleavage cycling amplification and planar intercalated methylene blue molecules amplification. The system showed high sensitivity and specificity, with the ability to distinguish different miRNA sequences, making it promising for clinical application in early cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Pavlos Nikolaou, Emanuele Luigi Sciuto, Alessandra Zanut, Salvatore Petralia, Giovanni Valenti, Francesco Paolucci, Luca Prodi, Sabrina Conoci
Summary: In this study, a novel molecular sensor based on electrochemiluminescence (ECL) was developed for ultrasensitive PCR-free detection of Hepatitis B Virus (HBV). The sensor showed an unprecedented low limit of detection (LoD) and can be applied to rapidly detect various infectious diseases, providing a new approach for future diagnostic screening.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Tianyu Du, Zewen Shi, Zhaojian Qin, Yiwei Hu, Yabin Zhu, Hui Jiang, Xuemei Wang
Summary: This study proposes an in situ targeted therapy strategy using a photothermally triggered nanoplatform. The nanoplatform, consisting of spherical nucleic acids, black phosphorus nanosheets, and zinc porphyrin metal-organic framework nanosheets, can disassemble during photothermal therapy, allowing for photothermally triggered photodynamic therapy. The enhanced singlet oxygen generation decreases heat shock proteins and improves the therapeutic effect. The superstructure shows potential as a synergistic platform for advanced cancer theranostics.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Analytical
Kai-Ren Zhao, Li Wang, Peng-Fei Liu, Xiao-Min Hang, Hui-Yi Wang, Shu-Ying Ye, Zhi-Jun Liu, Guo-Xi Liang
Summary: A novel electrochemiluminescence biosensor was developed for simple, sensitive, and quantitative detection of HIV and HPV-16 DNA. This system integrates spherical nucleic acid with CRISPR/Cas12a to achieve on-off signal-switchable detection of the two virus genes. The biosensor successfully avoids cross-interference and enables multiplex detection in human serum samples without the need for additional nucleic acid amplification steps.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Emily Kerr, Ryan Farr, Egan H. Doeven, Yi Heng Nai, Richard Alexander, Rosanne M. Guijt, Beatriz Prieto-Simon, Paul S. Francis, Megan Dearnley, David J. Hayne, Luke C. Henderson, Nicolas H. Voelcker
Summary: The sensor demonstrated in this study combines an ECL molecular beacon with magnetic bead based extraction of the miRNA target sequence to provide a sensitive and portable solution for miRNA-21 detection. The sensor does not require intercalating labels, reporter probes, added enzymes or hairpin primers for target amplification, offering a simple and robust strategy for miRNA detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Physical
Guangxin Xie, Jiekang Tian, Shuai Wei, Xingzuo Liu, Xuebing Ma
APPLIED CATALYSIS A-GENERAL
(2018)
Article
Chemistry, Physical
Bang Lin Li, Hao Lin Zou, Jie Kang Tian, Guo Chen, Xiao Hu Wang, Huan Duan, Xiao Lin Li, Yan Shi, Jing Rong Chen, Ling Jie Li, Jing Lei Lei, Hong Qun Luo, Nian Bing Li
Article
Chemistry, Physical
Dan Song, Jiekang Tian, Wen Xu, Hua Wen, Chao Wang, Ju Tang, Jin Zhang, Mingming Guo
Summary: This study examines the optoelectronic properties of dry fluorescent carbon quantum dots (FCQDs) using terahertz (THz) time-domain spectroscopy (TDS), revealing different behaviors of blue (B-CQDs) and red (R-CQDs) FCQDs in the THz range. This is the first research to apply THz TDS to dry CQDs, providing valuable insights into the physical properties of dry FCQDs.
Article
Biotechnology & Applied Microbiology
Yang Wang, Jun Chen, Jiekang Tian, Guanchen Wang, Weikang Luo, Zebing Huang, Yan Huang, Ning Li, Mingming Guo, Xuegong Fan
Summary: In this study, bio-friendly carbon quantum dots (TC-WS-CQDs) were synthesized and found to target hepatocellular carcinoma (HCC) cells and generate reactive oxygen species (ROS), leading to significant tumor inhibition through autophagy pathway. This study highlights a promising nanotheranostic strategy for cancer diagnosis and therapy.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Jiekang Tian, Yaohao Li, Bo Ma, Zhongping Tan, Shiying Shang
Summary: The development and application of commercially available automated peptide synthesizers have been crucial in peptide and protein research. Recent advances in peptide synthesis method and solid-phase chemistry have opened up new opportunities for enhancing the efficiency of these synthesizers. Successful synthesis of peptides with over 150 amino acid residues has proved useful in addressing the challenges of glycoprotein synthesis. This review provides an overview of the evolution of peptide synthesizers and glycoprotein synthesis, including the principles underlying different synthesizers, their strengths and weaknesses, and strategies to improve their applicability in glycoprotein synthesis.
FRONTIERS IN CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Li Sheng, Yanzhou Wu, Jiekang Tian, Li Wang, Jianlong Wang, Yaping Tang, Hong Xu, Xiangming He
Summary: By regulating the solvation structure of lithium ions, both the dendrite and parasitic reactions on lithium metal anodes can be suppressed, leading to improved battery performance. Lithium symmetric cells exhibit better performance at higher temperatures, with dendrite-free surfaces on cycled lithium metal anodes.
ENERGY & ENVIRONMENTAL MATERIALS
(2023)
