Article
Chemistry, Analytical
Qinqin Zhao, Xiang Ren, Shoufeng Wang, Dawei Fan, Nuo Zhang, Lei Liu, Yuyang Li, Qin Wei
Summary: The study proposes a new biosensor for the specific and precise detection of ATP using the CRISPR-Cas12a enzyme. The biosensor takes advantage of the enzyme's remarkable editability and sequence-specificity to amplify the signal for ATP detection. The CRISPR-Cas12a enzyme is able to trigger electrochemiluminescence (ECL) in the presence of ATP, allowing for accurate measurement of ATP concentration.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Lele Li, Siqi Yu, Jie Wu, Huangxian Ju
Summary: We developed a sensitive electrochemiluminescence (ECL) biosensing strategy for the detection of activated CRISPR/Cas12a using polymer dots (Pdots) as emitters. The strategy involves the regulation of binding between Pdots-DNA and a biosensor, and the enhancement of ECL emission through the local surface plasmon resonance (LSPR) effect of electrochemically deposited Au nanoparticles (depAuNPs). The integration of target DNA-triggered catalysis and the LSPR effect greatly improves the sensitivity of ECL analysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Yanling Huang, Weixin Li, Jianping Zheng, Fang Luo, Bin Qiu, Jian Wang, Cuiying Lin, Zhenyu Lin
Summary: In this study, a microchannel-based electrochemiluminescence biosensor was developed for the detection of adenosine triphosphate (ATP). The biosensor achieved high sensitivity and reliability by changing the charge density on the surface of microchannels. It was successfully applied to detect ATP in rat brain tissues.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biophysics
Kai Zhang, Zhenqiang Fan, Bo Yao, Yuedi Ding, Jing Zhao, Minhao Xie, Jianbin Pan
Summary: Siglec-5 is an inhibitory receptor for leukocytes in the human body, and the development of novel assays for Siglec-5 can aid in studying related disease pathogenesis and developing therapeutic drugs. A biosensor based on CRISPR-Cas12a and MXene has been developed for sensitive detection of Siglec-5, extending the application of CRISPR-Cas12a to biosensor construction and providing a common method for biomarker detection. This work represents the first investigation into the Trans-Cleavage activity of CRISPR-Cas12a for Mxene-based ECL biosensor establishment.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Xiuping Li, Xiujin Chen, Minxin Mao, Chifang Peng, Zhouping Wang
Summary: This study expands the target of CRISPR/Cas sensing to small molecules by integrating a bivalent aptamer as a recognition component. The detection speed of ATP is dramatically accelerated by shortening the binding time between the aptamer and ATP. Additionally, the integration of lateral flow assay with the CRISPR/Cas sensing strategy allows for visual detection of ATP.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Qinqin Zhao, Xiang Ren, Yu Du, Nuo Zhang, Yuyang Li, Dan Wu, Qin Wei
Summary: A method using a 3D dynamic DNA walker anchored in DNA micelles coupled with CRISPR-Cas12a nucleases sensor was proposed for the detection of microcystin-LR (MC-LR) through recovering electrochemiluminescence (ECL) signals. The DNA micelles provided stability and diffusion ability for the 3D DNA walker, while the Cas12a nucleases improved signal amplification and achieved higher sensitivity. The results confirmed the ability of DNA micelles to stabilize 3D DNA walkers and the validated cleavage activity of Cas12a nucleases.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Applied
Chunyan Wang, Yutian Zhang, Shanshan Liu, Yashi Yin, Gao-Chao Fan, Yizhong Shen, Heyou Han, Wenjing Wang
Summary: We developed an ECL sensor for Salmonella detection using allosteric probe as a bio-recognition element and CRISPR/Cas12a as a signal amplification strategy. The presence of Salmonella triggers structure switching on allosteric probes, leading to isothermal amplification. The released Salmonella initiates the next reaction cycle, generating dsDNA recognized by CRISPR-gRNA to activate Cas12a for trans-cleavage. The activated Cas12a cuts ssDNA bound to the electrode, resulting in the release of ECL emitter MOF and decreased ECL signal. The method shows high sensitivity in detecting Salmonella in real samples.
Article
Nanoscience & Nanotechnology
Yanling Huang, Weixin Li, Jianping Zheng, Fang Luo, Bin Qiu, Jian Wang, Cuiying Lin, Zhenyu Lin
Summary: In this study, a microchannel-based electrochemiluminescence (ECL) biosensor was used for the detection of adenosine triphosphate (ATP). The sensing performance of the biosensor relied on the change in charge density on the microchannel surface caused by a target recognition reaction. The system showed a linear relationship between ECL intensity and the logarithm of ATP concentration, with a detection limit of 0.32 fM.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Yaqi Liu, Fengyi Wang, Shuo Ge, Lu Zhang, Zuhao Zhang, Yunqing Liu, Yan Zhang, Shenguang Ge, Jinghua Yu
Summary: A strand displacement amplification (SDA)-assisted CRISPR/Cas12a electrochemiluminescence (ECL) biosensor was developed for ultrasensitive detection of Staphylococcus aureus (Sa)-16S rDNA. The biosensor utilized a porphyrinic Zr metal-organic framework (MOF) as a coreactant accelerator to enhance the ECL emission signal. The presence of Sa-16S rDNA triggered the SDA reaction, generating single-stranded DNA products (SP) that activated the CRISPR/Cas12a trans-cleavage and effectively extinguished the ECL signals. The ECL platform exhibited high sensitivity and specificity, making it suitable for clinical diagnosis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Huixin Zhang, Tingting Zhuang, Lun Wang, Lin Du, Jianfei Xia, Zonghua Wang
Summary: This study successfully designed a new strategy to form heterostructure Au NCs@Ti3C2 as a luminophore, greatly enhancing the ECL intensity. By combining with CHA and CRISPR-Cas12a, an ECL biosensor with good specificity and sensitivity for detecting miRNA-155 was constructed.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Zuhao Zhang, Lu Zhang, Shujing Wang, Ruifang Liu, Li Li, Peini Zhao, Chaomin Gao, Fengming Chen, Shenguang Ge, Jinghua Yu
Summary: A CRISPR/Cas12a electrochemiluminescence biosensor was developed for ultrasensitive detection of miRNA-21. The biosensor utilized Tribulus terrestris-like AuCu nanoterrestris and DNA tetrahedral nanostructures, and employed a multiple sites DNA walker for signal amplification, achieving efficient detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Cuiying Lin, Qingqing Huang, Mengjian Tian, Fang Luo, Jian Wang, Bin Qiu, Shuofei Yang, Zhenyu Lin
Summary: An electrochemiluminescence (ECL) biosensor with dual signal enhancement was developed to detect DNA adenine methylation methyltransferase (Dam MTase). The biosensor utilized a restriction endonuclease to cleave DNA and activate CRISPR/Cas12a trans-cleavage activity, resulting in enhanced ECL signal. The biosensor exhibited a good linear relationship with Dam MTase concentration and was successfully applied for detection in serum samples.
Article
Chemistry, Analytical
Huihui Yu, Haijian Yang, Weishuai Liu, Longsheng Jin, Bing Jin, Meisheng Wu
Summary: A novel portable and disposable bipolar electrode (BPE)-electrochemiluminescence (ECL) device was developed for fumonisin B1 (FB1) detection. The BPE was fabricated using MWCNTs and PDMS, and the ECL signal was greatly improved by depositing Au NPs on the cathode. The aptamer-based sensing strategy with Ag NPs as catalyst showed promising results for FB1 detection.
Article
Nanoscience & Nanotechnology
Qian Wang, Zuhao Zhang, Lu Zhang, Yunqing Liu, Li Xie, Shenguang Ge, Jinghua Yu
Summary: In this work, a CRISPR/Cas12a initiated switchable ternary electrochemiluminescence (ECL) biosensor combined with a Co3O4@Au nanoemitter is presented for the in vitro monitoring of miRNA-141. The biosensor demonstrated extraordinary sensitivity and specificity, with a low detection limit.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Li Li, Zheng Zhou, Xinmin Li, Dandan Li, Min Zhao, Changjin Liu, Haiping Wu, Wei Yang, Shijia Ding, Bo Shen
Summary: A novel 3D walking nanomachine-based biosensor utilizing strand displacement for ultrasensitive detection of ATP was successfully developed, demonstrating a low limit of detection and practical application in human serum samples. This biosensing strategy holds great potential for biochemical studies and clinical diagnosis.
Article
Geochemistry & Geophysics
Jintao Zhou, Wenchao Yu, Yuansheng Du, Xu Liu, Yuhang Wang, Guolin Xiong, Ziyuan Zhao, Dawei Pang, Daxing Shen, Shenfu Weng, Zhichen Liu, Deng Chen
Summary: This study provides new mineralogical, geochronological, and geochemical data on the bauxitic claystone in Guizhou Province, China, and determines its age and complex provenance. The study also highlights the importance of the paleogeographic location in the bauxitization process during the Late Permian in South China.
JOURNAL OF GEOCHEMICAL EXPLORATION
(2022)
Article
Materials Science, Ceramics
Dongjiang Wu, Xuexin Yu, Ziyuan Zhao, Guangyi Ma, Cong Zhou, Bi Zhang, Guanhui Ren, Fangyong Niu
Summary: Laser directed energy deposition (LDED) is used to fabricate TiCp-reinforced Al2O3-ZrO2 eutectic ceramics, with different ratios of TiCp percentage investigated on their inhibitory mechanism on crack and porosity defects. The distribution of TiCp particles and the microstructure around them are influenced by the solidification rate. The mismatch of thermal expansion coefficient and elastic modulus between TiCp and Al2O3-ZrO2 eutectic matrix causes crack pinning and transgranular, while TiCp doping enhances molten pool impact and reduces porosity.
CERAMICS INTERNATIONAL
(2023)
Article
Materials Science, Ceramics
Dongjiang Wu, Xuexin Yu, Ziyuan Zhao, Guangyi Ma, Cong Zhou, Bi Zhang, Guanhui Ren, Jun Han, Hong Wang, Fangyong Niu
Summary: Laser directed energy deposition (LDED) was used to manufacture TiCp reinforced Al2O3-ZrO2 eutectic functionally graded ceramics with two different transition modes for the first time. The results show that the gradient transition leads to a gradual increase of TiCp particles in the Al2O3-ZrO2 eutectic matrix. With increased TiCp content, the morphology of the eutectic matrix changes from lamellar or rod-shaped to irregular shape. LDED also enables controllable fabrication of properties in different positions of the gradient materials, with a 43.4% increase in wear resistance in TiCp rich regions compared to pure Al2O3-ZrO2 region.
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
(2023)
Article
Materials Science, Multidisciplinary
Ziyuan Zhao, Zheng Shen, Pan Dong, Yi He, Jianzuo Ma, Yanli Ma, Jiabin Yang, Weiguo Li
Summary: This study establishes a temperature-dependent predictive model to analyze the main influence mechanisms of nanoprecipitate-strengthened face-centered cubic high entropy alloys (NSFCC-HEAs) at different temperatures. The model successfully predicts the yield strength of NSFCC-HEAs at extremely cryogenic temperatures and quantitatively analyzes the contribution evolution of each mechanism with temperature. The influences of grain size, precipitate size, and volume fraction are also analyzed, and the applicability of the model is discussed.
METALS AND MATERIALS INTERNATIONAL
(2023)
Article
Computer Science, Artificial Intelligence
Fei Du, Yun Yang, Ziyuan Zhao, Zeng Zeng
Summary: This paper proposes a novel Efficient Perturbation Inference and Expandable Network (EPIE-Net) to address the issue of forgetting previous data in neural networks. The method dynamically expands task-specific decoders and utilizes a mixed-label uncertainty strategy to improve robustness. Experimental results demonstrate superior performance compared to other methods with fewer parameters in class incremental learning benchmarks.
Article
Cell Biology
Ya-Nan Zhao, Ping Wu, Zi-Yuan Zhao, Fei-Xiang Chen, Ao Xiao, Zhi-Yi Yue, Xin-Wei Han, Yong Zheng, Yun Chen
Summary: In this study, chitosan/graphene oxide (GO) films were prepared using an electrodeposition technique, and their effects on Schwann cell proliferation and adhesion abilities were evaluated. The results showed that the films promoted the expression of nerve regeneration-related factors and that a nerve guidance conduit with a GO content of 0.25% improved functional and histological outcomes in a rat sciatic nerve defect model.
NEURAL REGENERATION RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Ziyuan Zhao, Zirong Pan, Sen Zhang, Guodong Ma, Wen Zhang, Junke Song, Yuehua Wang, Linglei Kong, Guanhua Du
Summary: Stroke is a cerebrovascular disease caused by thrombus, with high morbidity and mortality rates. Neutrophils are the first cells to be recruited in the brain after stroke, and they aggravate brain injury through multiple mechanisms. Neutrophil extracellular traps (NETs) have been found to play important roles in the pathological process of stroke in recent years.
PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Computer Science, Information Systems
Zeng Zeng, Ziyuan Zhao, Kaixin Xu, Yangfan Li, Cen Chen, Xiaofeng Zou, Yulan Wang, Wei Wei, Pierce K. H. Chow, Xiaoli Li
Summary: In this paper, a new correlation induced clustering method (CoIn) is proposed to address the problem of high-dimensional bioinformatics data clustering. The method captures complex correlations among high-dimensional data and guarantees correlation consistency within each cluster. The evaluation on a high-dimensional mass spectrometry dataset of liver cancer tumor demonstrates that the proposed method produces more explainable and understandable results for clinical analysis, showing the potential application for knowledge discovery in high-dimensional bioinformatics data.
IEEE JOURNAL OF BIOMEDICAL AND HEALTH INFORMATICS
(2023)
Article
Materials Science, Multidisciplinary
Yuntao Wu, Yanli Ma, Pan Dong, Yi He, Ziyuan Zhao, Shifeng Zheng, Weiguo Li, Jianzuo Ma
Summary: Due to their high strength and high toughness, fine-grained metals have significant application value in aerospace and defense industries. This study proposes a temperature and grain size-dependent yield strength model for fine-grained metals considering the effect of grain boundary sliding. The model can predict the yield strength using easily obtained data and experimental results show good agreement, validating its rationality. The study also discusses the influences of Young's modulus and fracture toughness on yield strength at different temperatures and grain sizes, providing useful suggestions for preparing fine-grained metals with higher yield strength.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Computer Science, Interdisciplinary Applications
Ziyuan Zhao, Fangcheng Zhou, Kaixin Xu, Zeng Zeng, Cuntai Guan, S. Kevin Zhou
Summary: While deep learning methods have been successful in medical image segmentation, they are hindered by reliance on large-scale labeled datasets and failure to generalize across different domains. Unsupervised domain adaptation (UDA) techniques have been used to reduce the domain gap, but they degrade with limited source annotations. In this study, we propose a novel framework called Label-Efficient Unsupervised Domain Adaptation (LE-UDA) to address this problem and demonstrate its effectiveness in cross-modality segmentation.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2023)
Article
Chemistry, Organic
Chuan-Zhi Yao, Zu-Kui Xie, Jie-Yue Wang, Jia-Yu Zhang, Zi-Yuan Zhao, Qiankun Li, Jie Yu
Summary: Here, we present an anionic stereogenic-at-cobalt(III) complex catalysis strategy for the enantioselective halocyclization of ortho-alkynylanilines using N-halosuccinimide (NXS) as the halogen source. This method offers a distinct atroposelective approach to obtain axially chiral ortho-halo-C2-indole skeletons with excellent yields and good to high enantioselectivities (up to 99% yield, 99:1 er).
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jie Wang, Richard Chang, Ziyuan Zhao, Ramanpreet Singh Pahwa
Summary: Recent advancements in 3D deep learning have led to significant progress in improving accuracy and reducing processing time. This study utilizes the latest developments in 3D semi-supervised learning to create cutting-edge models for 3D object detection and segmentation in high-resolution X-ray semiconductors scans, achieving competitive performance and outperforming state-of-the-art methods. The use of contrastive learning and multi-scale Mean Teacher training further enhances the performance.
Article
Microbiology
Liying Sun, Liqin Wang, Chi Zhang, Yan Xiao, Lulu Zhang, Ziyuan Zhao, Lili Ren, Junping Peng
Summary: This study developed and evaluated a rapid, low-cost, expandable, and sequencing-free high-resolution melting (HRM) assay for the direct detection of SARS-CoV-2 variants. The assay accurately identified SARS-CoV-2 and differentiated between mutations within approximately 2 hours. It had a high specificity and sensitivity, showing promise as a valuable tool in detecting and monitoring SARS-CoV-2 variants.
MICROBIOLOGY SPECTRUM
(2023)
Article
Engineering, Mechanical
Qinyuan Yao, Jiabin Yang, Pan Dong, Ziyuan Zhao, Yi He, Yanli Ma, Feilong Zhang, Weiguo Li
Summary: Acoustic softening is a physically significant phenomenon with important applications, such as in ultrasonic-assisted metal processing and the use of metal materials in acoustic field environments. However, there is a lack of theoretical models to characterize the evolution of metal material strength with an acoustic field. This study developed two models, one for the yield strength and the other for the ultimate tensile strength, based on the equivalent relationship between acoustic energy and elastic deformation/elastoplastic strain energy. The models were validated by experimental results and used to analyze the effects of different acoustic fields on material strength.
EXTREME MECHANICS LETTERS
(2023)
Article
Mechanics
Pan Dong, Yanli Ma, Xuyao Zhang, Yi He, Ziyuan Zhao, Jianzuo Ma, Weiguo Li, Yile Li
Summary: A theoretical model characterizing the temperature and porosity dependent Young's modulus for micron particle reinforced metal matrix composites is established. Additionally, a temperature dependent yield strength analysis model is proposed by incorporating the influence of grain boundary slip on strengthening mechanisms and the yield strength. These models only require material parameters and temperature dependent specific heat capacity for application, and the predicted results are consistent with measured results. The effects of material parameters and mechanisms on Young's modulus and yield strength, as well as their variations with temperature, are explored based on these models.
COMPOSITE STRUCTURES
(2023)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)
