Article
Chemistry, Applied
Azam Bagheri Pebdeni, Maryam Mousavizadegan, Morteza Hosseini
Summary: The proposed dual detection sensor combines aptamer and antibiotic for effective recognition and quantification of Staphylococcus aureus. The sensor showed high specificity in detecting S. aureus using CuNCs, with potential applications for monitoring pathogenic bacteria.
Article
Chemistry, Analytical
Ruimeng Sun, Ruijuan Lv, Ting Du, Yuhan Li, Yang Zhang, Lixia Chen, Yanfei Qi
Summary: A freeze-thaw induced co-assembly of multi-enzyme-gold nanoparticle hybrids was developed for high-performance cascade reaction. This method has the advantages of simplicity, rapidity, low cost, and environmental friendliness, and can be used to construct a sensitive and speedy colorimetric sensor for glucose detection.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Ruimeng Sun, Xiaoyu Zhang, Ting Du, Yuhan Li, Hainan Ma, Lina Zhang, Haolin Sun, Yanfei Qi
Summary: In this study, a detection platform utilizing the aggregation of gold nanoparticles (AuNPs) through freeze-thaw and the fluorescence resonance energy transfer (FRET) was proposed for identifying Staphylococcus aureus. The method demonstrated a rapid and sensitive analysis of S. aureus, with a significantly shortened detection time.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Yadi Qin, Hayilati Bubiajiaer, Jun Yao, Minwei Zhang
Summary: Residue and illegal addition of Dexamethasone (DEX) in food has attracted widespread attention, and there is a need for highly sensitive and rapid detection methods. In this study, a colorimetric sensor based on an unmodified aptamer and gold nanoparticles was designed to detect DEX in milk and glucosamine.
Article
Chemistry, Multidisciplinary
Yuan Liu, Taotao Li, Gaojian Yang, Yan Deng, Xianbo Mou, Nongyue He
Summary: The authors developed a simple gold nanoparticles-based colorimetric biosensor for chlorpyrifos detection, utilizing an aptamer as the capture probe. The method exhibited excellent sensitivity and reliable detection performance, making it a promising tool for chlorpyrifos detection in water and agricultural products.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Analytical
Minghui Shi, Ruobing Liu, Fuyuan Zhang, Bimal Chitrakar, Xianghong Wang
Summary: In this study, single-stranded DNA (ssDNA) aptamers specific to florfenicol (FF) with high binding affinity were prepared using the magnetic bead-based systematic evolution of ligands by the exponential enrichment technique (MB-SELEX). The optimized aptamer Apt-14t, with a length of 43 nt, showed a dissociation constant 7 times higher than the full-length sequence. Molecular docking simulations confirmed the potential binding sites and interactions with FF. Additionally, a colorimetric strategy for nanogold aptamers was developed, demonstrating high accuracy in detecting florfenicol in milk and eggs samples.
Article
Chemistry, Analytical
Xiaoyu Qi, Yufei Ye, Hao Wang, Bianbian Zhao, Lixia Xu, Yuting Zhang, Xiaoli Wang, Nandi Zhou
Summary: An ultrasensitive and dual-recognition surface-enhanced Raman scattering (SERS) biosensor has been developed for the detection of Staphylococcus aureus (S. aureus). The biosensor utilizes teicoplanin (Tcp) functionalized gold-coated magnet nanoparticles (Fe3O4@Au-Tcp NPs) as the capture probe and S. aureus aptamer (Apt) functionalized silver coated gold nanoparticles (Au@Ag-DTNB-Apt NPs) as the signal probe. The biosensor demonstrates high sensitivity and specificity due to the SERS enhancement from the hot spot effect between Au NPs and Au@Ag NPs, as well as the dual-recognition ability based on Tcp and Apt. The biosensor is capable of detecting S. aureus in milk and orange juice samples.
Article
Chemistry, Analytical
Yan Huang, Xiaoying Pei, Shina Du, Zhongfang Li, Xiangling Gu, Wei Sun, Xueliang Niu
Summary: In this study, we designed and explored a simple ratiometric electrochemical aptasensor for thrombin detection using a signal on-off strategy. We synthesized and characterized AuNPs-MXene as a signal amplified sensing platform, and introduced hairpin probes labeled with ferrocene and thrombin aptamer labeled with methyl blue to fabricate a highly selective and ratiometric dual-signal readout aptasensor.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Miao Zhang, Yin Chen, Shi Gang Liu, Xingbo Shi
Summary: A highly efficient and sensitive electrochemical biosensor was developed for the monitoring of L. m contamination, which has a fast preparation time and good linear range and detection limit, and has been successfully applied to actual samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Yanwei Wang, Simone Rink, Antje J. Baeumner, Michael Seidel
Summary: Gold nanoparticle-catalyzed chemiluminescence of luminol was used for the detection of sulfadimethoxine (SDM) on a microfluidic platform. A 3D microfluidic mixer design was optimized to ensure efficient mixing of components. The use of a more hydrophilic luminol derivative led to significantly enhanced signal and reliable results. The detection platform showed a wide dynamic range and a low limit of detection, making it a promising strategy for rapid and cost-efficient analysis in environmental monitoring and food safety.
Article
Chemistry, Multidisciplinary
Bo Chen, Lan Mei, Rangrang Fan, Yuelong Wang, Chunlai Nie, Aiping Tong, Gang Guo
Summary: Stimuli-responsive DNA nanostructure-based nanodevices have been utilized for cancer therapy by constructing drug nanocarriers with pH-responsive i-motif DNA on gold nanoparticles. The multifunctional drug carrier demonstrated potential for synergistic photothermal-chemotherapy, achieving efficient anticancer efficiency through targeted cellular uptake and the synergistic effect between chemotherapy and photothermal therapy utilizing the excellent photothermal conversion efficiency of AuNPs under near-infrared irradiation.
CHINESE CHEMICAL LETTERS
(2021)
Article
Food Science & Technology
Ruobing Liu, Fuyuan Zhang, Yaxin Sang, Minxuan Liu, Minghui Shi, Xianghong Wang
Summary: An ssDNA aptamer (Apt-5) with high affinity and specificity for AFM1 was successfully isolated and used to detect AFM1 in milk and dairy products.
Article
Chemistry, Analytical
Wanyi Xie, Shixuan He, Shaoxi Fang, Liyuan Liang, Biao Shi, Deqiang Wang
Summary: A simple and effective fluorescence platform using nanopipette has been established for visualizing nanomaterials' protective effect on DNA, achieving sensitive detection of MC-LR and signal amplification. The interaction between aptamer/AuNPs prevents DNA cleavage at the nanoscale confinement and provides new kinetic information about dynamic chemical processes.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Meng Tian, Jihua Wang, Chonghui Li, Zhenxing Wang, Guofeng Liu, Enguang Lv, Xiaofei Zhao, Zhen Li, Dongyan Cao, Huilan Liu, Chao Zhang, Shicai Xu, Baoyuan Man
Summary: In this study, a biosensor based on SERS and FET dual sensing mode was developed for the detection of MC-LR. The sensor showed a low detection limit and high specificity, making it a promising tool for water environment detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Immunology
Pernille Ommen, Line Hansen, Bente K. Hansen, Hieu Vu-Quang, Jorgen Kjems, Rikke L. Meyer
Summary: In this study, an aptamer-targeted liposomal drug delivery system was developed to eradicate Staphylococcus aureus biofilm infections in vitro.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)