期刊
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
卷 157, 期 4, 页码 546-553出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/ajcp/aqab148
关键词
Machine learning; Flow cytometry; Acute promyelocytic leukemia; Acute myeloid leukemia; B-cell lymphoblastic leukemia; lymphoma
类别
Flow cytometry is crucial for hematologic malignancies diagnosis, and machine learning methods can greatly enhance the efficiency of data analysis. The study aimed to build a model to classify acute leukemias, including APL, and highlighted the significant contribution of light scatter properties in achieving high accuracy.
Objectives Flow cytometry (FC) is critical for the diagnosis and monitoring of hematologic malignancies. Machine learning (ML) methods rapidly classify multidimensional data and should dramatically improve the efficiency of FC data analysis. We aimed to build a model to classify acute leukemias, including acute promyelocytic leukemia (APL), and distinguish them from nonneoplastic cytopenias. We also sought to illustrate a method to identify key FC parameters that contribute to the model's performance. Methods Using data from 531 patients who underwent evaluation for cytopenias and/or acute leukemia, we developed an ML model to rapidly distinguish among APL, acute myeloid leukemia/not APL, acute lymphoblastic leukemia, and nonneoplastic cytopenias. Unsupervised learning using gaussian mixture model and Fisher kernel methods were applied to FC listmode data, followed by supervised support vector machine classification. Results High accuracy (ACC, 94.2%; area under the curve [AUC], 99.5%) was achieved based on the 37-parameter FC panel. Using only 3 parameters, however, yielded similar performance (ACC, 91.7%; AUC, 98.3%) and highlighted the significant contribution of light scatter properties. Conclusions Our findings underscore the potential for ML to automatically identify and prioritize FC specimens that have critical results, including APL and other acute leukemias.
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