期刊
ACS APPLIED MATERIALS & INTERFACES
卷 14, 期 8, 页码 10844-10855出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.1c24475
关键词
SARS-CoV-2; antigen testing; nucleocapsid proteins; zinc oxide; reduced graphene oxide; electrochemical immunosensing
资金
- Canadian Institutes of Health Research (CIHR)
- Natural Sciences and Engineering Research of Canada (NSERC)
- Canada Research Chair
- CMC Microsystems, Canada
- University of Calgary
This study presents a stable buffer-based zinc oxide/reduced graphene oxide nanocomposite coated on carbon screen-printed electrodes for the electrochemical immuno-biosensing of SARS-CoV-2 protein antigens. The biosensor shows a low limit of detection, stable sensor nanocoating, high sensitivity, and the ability to rapidly discriminate positive and negative clinical samples.
The widespread and long-lasting effect of the COVID-19 pandemic has called attention to the significance of technological advances in the rapid diagnosis of SARS-CoV-2 virus. This study reports the use of a highly stable buffer-based zinc oxide/reduced graphene oxide (bbZnO/rGO) nanocomposite coated on carbon screen-printed electrodes for electrochemical immuno-biosensing of SARS-CoV-2 nuelocapsid (N-) protein antigens in spiked and clinical samples. The incorporation of a salt-based (ionic) matrix for uniform dispersion of the nanomixture eliminates multistep nanomaterial synthesis on the surface of the electrode and enables a stable single-step sensor nanocoating. The immuno-biosensor provides a limit of detection of 21 fg/mL over a linear range of 1-10 000 pg/mL and exhibits a sensitivity of 32.07 ohms.mL/pg.mm(2) for detection of N-protein in spiked samples. The N-protein biosensor is successful in discriminating positive and negative clinical samples within 15 min, demonstrating its proof of concept used as a COVID-19 rapid antigen test.
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