Article
Biochemistry & Molecular Biology
Leonardo M. Fabri, Cintya M. Moraes, Maria I. C. Costa, Daniela P. Garcon, Carlos F. L. Fontes, Marcelo R. Pinto, John C. McNamara, Francisco A. Leone
Summary: This study investigated the regulation of gill (Na+, K+)-ATPase activity in freshwater crustaceans, finding that protein kinases A and C, as well as FXYD2 peptide, play a role in modulating enzyme activity through phosphorylation mechanisms instead of through the expression of different isoforms.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Article
Fisheries
Yaqiu Liu, Zhijian Wang
Summary: This study explored the gill developmental mechanism of high larval mortality in Paramisgurnus dabryanus. The results showed that external gills appeared 12 hours after hatching and the number of external branchial filaments greatly increased at 2 days after hatching. Internal branchial filament primordial was formed at 5 days after hatching and by 9 days after hatching, external branchial filaments were not observed outside. Gill filament and lamellae increased significantly in number and length from 15 days after hatching. The specific activities of Na+/K+-ATPase steadily increased until 12 days after hatching and then slightly decreased. Abundant gill lamellae were observed at 40 days after hatching. The sensitive period of larval and juvenile P. dabryanus development was from 6 to 15 days after hatching.
Article
Biochemistry & Molecular Biology
Leonardo M. Fabri, Daniela P. Garcon, Cintya M. Moraes, Marcelo R. Pinto, John C. McNamara, Francisco A. Leone
Summary: This investigation examines the kinetic characteristics and effect of acclimation to a brackish medium on gill V(H+)-ATPase activity in two hololimnetic populations of M. amazonicum. The study also investigates the cellular immunolocalization of the enzyme. The findings demonstrate the distribution of V(H+)-ATPase c-subunit in shrimps' pillar cells in fresh water and its absence after acclimation to a brackish medium. The importance of crustacean V(H+)-ATPase for ion uptake in fresh water is highlighted.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rebeca Acin-Perez, Cristiane Beninca, Lucia Fernandez del Rio, Cynthia Shu, Siyouneh Baghdasarian, Vanessa Zanette, Christoph Gerle, Chimari Jiko, Ramzi Khairallah, Shaharyar Khan, David Rincon Fernandez Pacheco, Byourak Shabane, Karel Erion, Ruchi Masand, Sundeep Dugar, Cristina Ghenoiu, George Schreiner, Linsey Stiles, Marc Liesa, Orian S. Shirihai
Summary: The regulation of ATP synthesis and hydrolysis is crucial for cellular function. In this study, we investigate the hydrolytic activity of mitochondrial ATP synthase (CV) and its impact on cellular energetics. We identify a selective inhibitor of ATP hydrolysis, (+)-Epicatechin, that binds CV and prevents the binding of ATPase inhibitor (ATPIF1). By inhibiting CV hydrolytic activity, ATP content is restored in cells with respiratory chain defects or Duchenne Muscular Dystrophy, improving muscle force without increasing mitochondrial content. These findings highlight the potential of hydrolysis-selective inhibitors of CV in mitigating the effects of compromised mitochondrial respiration.
Editorial Material
Biochemistry & Molecular Biology
Hector Miranda-Astudillo, Mariel Zarco-Zavala, Jose J. Garcia-Trejo, Diego Gonzalez-Halphen
Summary: The central rotor of F1Fo-ATP synthase can rotate clockwise or counterclockwise, functioning as either ATP synthase or ATP hydrolase. The regulation by inhibitory proteins helps preserve the key function of ATP synthesis. The study highlights the inhibitory role of the epsilon subunit in bacterial F1Fo-ATP synthase.
Article
Chemistry, Multidisciplinary
Mrinal Shekhar, Chitrak Gupta, Kano Suzuki, Chun Kit Chan, Takeshi Murata, Abhishek Singharoy
Summary: The mechanism of rotatory catalysis in ATP hydrolyzing molecular motors is still poorly understood. In this study, a complete 4-step conformational cycle model of rotatory catalysis is proposed using vacuolar or V-1-type hexameric ATPase as an example. A new intermediate or dwell is identified and a putative cyclic rotation path is derived. Free-energy simulations estimate the rate of protein transformations and reveal the role of arginine-finger residues in coupling chemical and mechanical information. Analysis indicates that overcoming the ADP interactions with V-1-ATPase is the rate-limiting step of motor action.
ACS CENTRAL SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Zongjie Dai, Yan Zhu, Hongjun Dong, Chunhua Zhao, Yanping Zhang, Yin Li
Summary: Depletion of intracellular ATP significantly increased glycolytic rate, enhanced solvent production, and resulted in a wide range of metabolic changes in C. acetobutylicum DSM 1731. This finding provides a novel strategy for engineering solvent-producing C. acetobutylicum and other anaerobic microbial cell factories.
MICROBIAL CELL FACTORIES
(2021)
Article
Microbiology
Andrea Lopez, Maria Andreadaki, Juha B. Vahokoski, Elena Deligianni, Lesley Calder, Serena Camerini, Anika K. Freitag, Ulrich Bergmann, Peter K. Rosenthal, Inga Siden-Kiamos, Inari K. Kursula
Summary: Actin I and Actin II in malaria parasites have different structures and functions, with Actin I playing a key role in motility and Actin II being involved in male gametogenesis and the oocyst stage. Actin II readily forms long filaments in vitro and has similar structures to Actin I. Mutational analysis reveals that long and stable filaments are necessary for male gametogenesis, and fine-tuned regulation by methylation of histidine 73 is required for the second function in the oocyst stage. Actin II polymerizes via the nucleation-elongation mechanism, and dimers are a stable form at equilibrium.
Article
Biology
Sarina Veit, Thomas Guenther Pomorski
Summary: ATPase assays are commonly used for the characterization of purified ATPases. This radioactive [γ32P]-ATP-based assay, utilizing complex formation with molybdate, offers high sensitivity and can be used for proteins with low ATPase activity or low purification yields. It has various applications including substrate identification, determination of the effect of mutations on ATPase activity, and testing of ATPase inhibitors.
Article
Biology
Sarina Veit, Thomas Gunther Pomorski
Summary: ATPase assays are commonly used for characterizing purified ATPases. This radioactive [gamma-P-32]-ATP-based approach described here can be highly sensitive and is suitable for proteins with low ATPase activity or low purification yields. It can be used for various applications including substrate identification, determining the effect of mutations on ATPase activity, and testing ATPase inhibitors.
Article
Biochemistry & Molecular Biology
Sunil Nath
Summary: The integration of phosphorus chemistry with the mechanism of ATP synthesis/hydrolysis requires dynamical information during ATP turnover and catalysis. Oxygen exchange reactions occurring at beta-catalytic sites of the FOF1-ATP synthase/F-1-ATPase imprint a unique record of molecular events during the catalytic cycle of ATP synthesis/hydrolysis. The present work conducts new experiments on oxygen exchange catalyzed by submitochondrial particles designed to (i) measure the relative rates of Pi-ATP, Pi-HOH, and ATP-HOH isotope exchanges; (ii) probe the effect of ADP removal on the extent of inhibition of the exchanges, and (iii) test their uncoupler sensitivity/resistance. The objectives have been realized based on new experiments on submitochondrial particles, which show that both the Pi-HOH and ATP-HOH exchanges occur at a considerably higher rate relative to the Pi-ATP exchange, an observation that cannot be explained by previous mechanisms.
Article
Biochemistry & Molecular Biology
Ronald B. Brown
Summary: Nearly half of sudden cardiac arrest deaths occur in individuals without any detectable heart disease, and around one-third of sudden cardiac arrest deaths in children and young adults remain unexplained after thorough examination. This perspective paper proposes a pathophysiological mechanism in which phosphate toxicity interferes with normal calcium handling in the heart, leading to sudden cardiac arrest.
Article
Chemistry, Multidisciplinary
Sunil Nath
Summary: F1-ATPase is a small motor that rotates in 120o steps fueled by ATP hydrolysis. This study reveals that the rotation is driven by the electrostatic free energy change associated with ATP cleavage reaction and Pi release. This finding has important implications for understanding the mechanical working mechanism of F1-ATPase and the transduction of free energy in biomolecular systems.
FRONTIERS IN CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sunil Nath
Summary: This study introduces a new theory of oxygen exchange and applies it to ATP hydrolysis by the mitochondrial F-1-ATPase. The results suggest that steady-state ATP hydrolysis by F-1-ATPase occurs with all three sites occupied by Mg-nucleotide, contradicting previous bisite models and supporting the trisite catalysis formulated by Nath's torsional mechanism.
Review
Pharmacology & Pharmacy
Adrienne R. Guarnieri, Tyler W. Benson, Michael Tranter
Summary: This review discusses the role of SERCA-mediated calcium cycling as a significant mediator of thermogenesis in both brown and beige adipocytes, proposing two primary mechanisms: direct uncoupling of ATPase and calcium pump activity, and futile cycling of SR calcium.
MOLECULAR PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mariana Vellosa Capparelli, Isabella C. Bordon, Giuliana Araujo, Paloma Kachel Gusso-Choueri, Denis Moledo de Souza Abessa, John Campbell McNamara
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2019)
Article
Environmental Sciences
M. Capparelli, J. C. McNamara, M. G. Grosell
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
(2020)
Article
Physiology
Samuel Coelho Faria, Adalto Bianchini, Mariana Machado Lauer, Ana Lucia Ribeiro Latorre Zimbardi, Federico Tapella, Maria Carolina Romero, John Campbell McNamara
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Environmental Sciences
M. V. Capparelli, J. C. McNamara, G. S. Araujo, A. C. F. Cruz, D. M. S. Abessa
Summary: The study compared sediment quality in estuaries at three locations along the coast of Sao Paulo State, Brazil, during two seasons of 2013. Results showed that sediments from the most protected locality had lower metal pollution and toxicity compared to those from less protected areas, which exhibited signs of environmental degradation.
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Francisco A. Leone, Malson N. Lucena, Leonardo M. Fabri, Daniela P. Garcon, Carlos F. L. Fontes, Rogerio O. Faleiros, Cintya M. Moraes, John C. McNamara
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2020)
Article
Biology
Milene Mantovani, John Campbell McNamara
Summary: The study revealed that the freshwater crab Pagei survives in fresh water by maintaining cellular isosmoticity, while the shrimp Jelskii exhibits clear hypo-osmoregulatory ability. Each of the two species has evolved distinct transcriptional and systemic strategies to adapt to the freshwater environment.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Marine & Freshwater Biology
Mariana V. Capparelli, Carl L. Thurman, Paloma Gusso Choueri, Denis Moledo de Souza Abessa, Mayana Karoline Fontes, Caio Rodrigues Nobre, John Campbell McNamara
Summary: This study compared the physiological and biochemical adjustments of two fiddler crab species from the Galapagos archipelago to submersion and desiccation challenges, revealing species-specific survival and stress responses. The integrated biomarker response index based on oxidative stress indicated higher oxidative stress in Leptuca helleri, while submersion had a greater impact on Minuca galapagensis.
Article
Marine & Freshwater Biology
Daniela P. Garcon, Francisco A. Leone, Rogerio O. Faleiros, Marcelo R. Pinto, Cintya M. Moraes, Leonardo M. Fabri, Claudia D. Antunes, John Campbell McNamara
Summary: Salt tolerance in Brachyura is closely related to their ability to adjust body fluid concentrations, with mechanisms relying on the gill (Na+, K+)-ATPase. Callinectes danae demonstrates intricate osmotic and chloride regulation abilities under different salinity conditions, which are linked to variations in ATPase activity, protein expression, and mRNA expression.
Article
Biochemistry & Molecular Biology
Anieli Cristina Maraschi, Samuel Coelho Faria, John Campbell McNamara
Summary: This study focused on the ion regulation capabilities of palaemonid shrimps in different salinity environments. Palaemonids exhibit hypo-regulation in high salinity and hyper-regulation in low salinity. The research revealed a positive correlation between the expression of gill NKCC protein and the hemolymph's hyper-osmoregulatory capacity, suggesting a role for NKCC in salt secretion.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Environmental Sciences
Mariana V. Capparelli, John C. McNamara, Carl L. Thurman, Rosela Perez-Ceballos, Mario A. Gomez-Ponce, Jose-Gilberto Cardoso-Mohedano, Gabriel M. Moulatlet
Summary: We assessed the responses of mangrove crabs to global climate changes and found that more terrestrial species were better adapted to survive in water, while less terrestrial species were more adapted to live on land.
MARINE POLLUTION BULLETIN
(2022)
Article
Zoology
John Campbell McNamara, Carolina Arruda Freire
Summary: Early marine invertebrates adapted to dilute media by acquiring osmoregulatory abilities. These adaptations include reduced body permeability, lowered osmotic concentrations, and increased osmotic gradients. Assessing freshwater invertebrates that have successfully invaded this environment, we find diverse osmoregulatory characteristics influenced by body plans, morpho-physiological resources, and occupation routes.
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Leonardo M. Fabri, Cintya M. Moraes, Maria I. C. Costa, Daniela P. Garcon, Carlos F. L. Fontes, Marcelo R. Pinto, John C. McNamara, Francisco A. Leone
Summary: This study investigated the regulation of gill (Na+, K+)-ATPase activity in freshwater crustaceans, finding that protein kinases A and C, as well as FXYD2 peptide, play a role in modulating enzyme activity through phosphorylation mechanisms instead of through the expression of different isoforms.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Article
Zoology
Samuel Coelho Faria, John Campbell McNamara
Summary: Weakly osmoregulating crustaceans use intracellular free amino acids (FAA) to attenuate cell volume changes, but it is unknown if semiterrestrial, strong hyper/hypo-osmoregulators have this ability. This study investigates FAA mobilization in muscle tissue of 10 fiddler crabs from different genera along the Atlantic coast of South America. The results show that hypo-osmoregulating crabs can mobilize FAA, likely due to a lesser ability to secrete salt near their upper critical limits. On the other hand, hyper-osmoregulating crabs have a strong extracellular regulatory ability, so FAA plays a diminished role in isosmotic intracellular regulation.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
(2023)
Article
Biology
John Campbell McNamara, Anieli Cristina Maraschi, Federico Tapella, Maria Carolina Romero
Summary: This study examines the osmoregulatory characteristics and gene expression of two sub-Antarctic Eubrachyura crabs in low temperature environments. The results show that while both crabs exhibit similar systemic osmoregulatory characteristics, their gene expression for ion uptake and secretion differs significantly.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2023)
Meeting Abstract
Zoology
J. C. McNamara, C. A. Freire
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2023)