Article
Biochemistry & Molecular Biology
Theresa A. Engelmann, Lars Knudsen, Dominik H. W. Leitz, Julia Duerr, Michael F. Beers, Marcus A. Mall, Matthias Ochs
Summary: Conditional Nedd4-2 deficiency in adult mice leads to impaired mucociliary clearance and accumulation of Muc5b, ultimately resulting in progressive pulmonary fibrosis. Ultrastructural alterations of the alveolar epithelium are correlated with interstitial remodeling, with abnormal regeneration of the alveolar epithelium contributing to the process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Hanne Beeckmans, Gene P. L. Ambrocio, Saskia Bos, Astrid Vermaut, Vincent Geudens, Arno Vanstapel, Bart M. Vanaudenaerde, Frans De Baets, Thomas L. A. Malfait, Marie-Paule Emonds, Dirk E. Van Raemdonck, Helene M. Schoemans, Robin Vos, Leuven Lung Transplant Grp
Summary: Pulmonary alveolar proteinosis (PAP) is a rare lung disorder characterized by surfactant accumulation, and lung transplantation followed by hematopoietic stem cell transplantation may be an effective treatment for end-stage hereditary PAP-induced pulmonary fibrosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Chao Hong, Hongyun Lu, Rong Jin, Xiaohong Huang, Ming Chen, Xiaoqiu Dai, Fangyuan Gong, Hongliang Dong, Hongmin Wang, Xiao-Ming Gao
Summary: The lack of IL-13-secreting ILC2s in the lungs may be responsible for poor AM reconstitution post haplo-BMT. Supplementing with factors like IL-13 can enhance donor monocyte-derived AM reconstitution and innate immunity against pulmonary bacterial infection in recipient animals. These findings offer a potential clinical strategy for preventing early pulmonary bacterial infection in BMT recipients.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Dazhen Jiang, Di Deng, Yu Xiong, Dajiang Wang, Jian Gong, Hongli Zhao, Zhirong Bao, Yongchang Wei, Conghua Xie, Lecheng Jia, Can Liao, Shuo Liu, Hui Liu, Xiaoyong Wang
Summary: This study reports on the clinical routine of performing TMLI treatment on the CT-linac and compares the dose distribution between planned and re-calculated based on IGRT FBCT image sets. The results show a significant difference between planned and delivered doses, as well as a discrepancy in the dose received by selected organs. However, the overall treatment was delivered with adequate target coverage.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Hematology
Hannah Kinoshita, Mamatha Mandava, Mariah Jensen-Wachspress, Haili Lang, Elisabeth Joy, Jay Tanna, Chase D. McCann, Samuel O'Brien, Sianna Burnett, Abeer Shibli, Fahmida Hoq, Monica Bhatia, Patrick J. Hanley, Blachy Davila Saldana, Kris M. Mahadeo, Catherine M. Bollard, Michael D. Keller, Allistair Abraham
Summary: Hematopoietic stem cell transplantation (HSCT) is increasingly used to treat sickle cell disease (SCD). However, this treatment increases the risk of viral infection. This study explores the use of virus-specific T-cell (VST) therapies after HSCT to prevent or treat viral infections in SCD patients.
Article
Medicine, General & Internal
David I. Marks, Anna Castleton, Eduardo Olavarria, Maria Gilleece, Adele Fielding, George Mikhaeel, Matthew Beasley, Patricia Diez, Aimee Jackson, Andrea Hodgkinson, Mohamed Elhanied, Ronjon Chakraverty
Summary: This study examines the usage of T-cell depleted, reduced intensity conditioning (RIC) approach to haematopoietic cell transplantation (HCT) in adult patients with acute lymphoblastic leukaemia (ALL). The results show encouraging survival rates but high relapse rates, suggesting the need for more powerful conditioning approaches.
Article
Oncology
Ji Eun Lim, Srideshikan Sargur Madabushi, Paresh Vishwasrao, Joo Y. Song, Amr M. H. Abdelhamid, Hemendra Ghimire, V. L. Vanishree, Jatinder K. Lamba, Savita Dandapani, Amandeep Salhotra, Mengistu Lemecha, Antonio Pierini, Daohong Zhao, Guy Storme, Shernan Holtan, Cynthia Aristei, Dorthe Schaue, Monzr Al Malki, Susanta K. Hui
Summary: Total marrow irradiation (TMI) is an emerging radiation conditioning regimen that can overcome the limitations of total body irradiation (TBI) by reducing normal tissue damage and preserving repair capacity. Studies have shown that dose escalation using TMI significantly improves survival rates in relapsed/refractory AML patients. Preclinical experiments on mice demonstrated that TMI does not affect donor engraftment, but reduces normal tissue damage and enhances repair processes, suggesting the potential for dose escalation in elderly patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Srideshikan Sargur Madabushi, Raghda Fouda, Hemendra Ghimire, Amr M. H. Abdelhamid, Ji Eun Lim, Paresh Vishwasrao, Stacy Kiven, Jamison Brooks, Darren Zuro, Joseph Rosenthal, Chandan Guha, Kalpna Gupta, Susanta K. Hui
Summary: Sickle cell disease is a serious global health problem and the only curative option is hematopoietic stem cell transplant. Reduced-intensity total body radiation is currently used as a conditioning regimen and targeted marrow irradiation is a novel technique that can protect organs and effectively treat some patients.
FRONTIERS IN ONCOLOGY
(2022)
Review
Oncology
Jeffrey Y. C. Wong, An Liu, Chunhui Han, Savita Dandapani, Timothy Schultheiss, Joycelynne Palmer, Dongyun Yang, George Somlo, Amandeep Salhotra, Susanta Hui, Monzr M. M. Al Malki, Joseph Rosenthal, Anthony Stein
Summary: TMI utilizes IMRT to deliver organ sparing targeted radiotherapy in patients undergoing hematopoietic cell transplantation (HCT). Over 500 patients have received TMI at this center, with studies showing low toxicities and non-medullary relapse rates.
FRONTIERS IN ONCOLOGY
(2022)
Article
Acoustics
Yunji Wang, Jie Li, Jianpin Zhou, Ye Qiu, Jinlin Song
Summary: Through experimental research, it was found that the combination therapy of low-intensity pulsed ultrasound (LIPUS) and bone marrow stromal cells (BMSCs) can enhance alveolar bone regeneration in periodontal diseases, achieving better therapeutic effects.
Article
Oncology
Prema Rassiah, Natia Esiashvili, Arthur J. Olch, Chia-Ho Hua, Ken Ulin, Andrea Molineu, Karen Marcus, Mahesh Gopalakrishnan, Susha Pillai, Nataliya Kovalchuk, An Liu, Greg Niyazov, Jose Penagaricano, Fred Cheung, Adam C. Olson, Cheng-Chia Wu, Harish K. Malhotra, Iain J. MacEwan, Jacqueline Faught, John C. Breneman, David S. Followill, Thomas J. FitzGerald, John A. Kalapurakal
Summary: The study examined current practice patterns in pediatric total body irradiation (TBI) techniques among COG member institutions. Results showed inconsistencies in practice patterns and a lack of standardization, making it difficult to correlate TBI doses with clinical outcomes effectively.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2021)
Article
Critical Care Medicine
Zhenmei Zhang, Can Wang, Steve G. Peters, William J. Hogan, Shahrukh K. Hashmi, Mark R. Litzow, Mrinal S. Patnaik, Alexander S. Niven, Hemang Yadav
Summary: DAH after HCT has a low incidence rate but high mortality rate, requiring attention. Multiple factors may influence the incidence rate, mortality rate, and risk factors, including time of onset, platelet count, and the need for invasive mechanical ventilation. For treatment outcomes, lower doses of steroids may help improve patient survival.
Article
Dentistry, Oral Surgery & Medicine
Alshaimaa Ahmed Shabaan, Ahmad Salahuddin, Inass Aboulmagd, Reham Ragab, Khaled Amr Salah, Adel Rashid, Haytham Mohamed Ayad, Walaa Abd el Aty Ahmed, Shaimaa Mohsen Refahee
Summary: This study compared the bone density and volume in patients with alveolar cleft reconstructions using a combination of bone marrow aspirate concentrate with iliac graft versus iliac graft alone. The results showed that using bone marrow concentrate can significantly increase bone volume and density while reducing bone loss ratio.
CLINICAL ORAL INVESTIGATIONS
(2023)
Review
Medicine, General & Internal
Mariana Nassif Kerbauy, Leonardo Javier Arcuri, Sergio Leonardo Favareto, Ana Carolina Pires de Rezende, Nelson Hamerschlak
Summary: Total body irradiation (TBI) is an important part of the conditioning regimen in hematopoietic cell transplantation. However, higher doses of TBI can result in more significant toxicities. To overcome this, total marrow irradiation and total marrow and lymphoid irradiation have been developed to deliver targeted radiotherapy while sparing organs. Studies have shown that these techniques can be safely administered with different chemotherapy conditioning regimens, especially in situations such as multiple myeloma, high-risk hematologic malignancies, relapsed or refractory leukemias, and elderly or frail patients, with low transplant-related mortality rates.
FRONTIERS IN MEDICINE
(2023)
Article
Clinical Neurology
Antonio Uccelli, Alice Laroni, Rehiana Ali, Mario Alberto Battaglia, Morten Blinkenberg, Lou Brundin, Michel Clanet, Oscar Fernandez, James Marriot, Paolo Muraro, Seyed Massood Nabavi, Roberto S. Oliveri, Ernst Radue, Cristina Ramo Tello, Irene Schiavetti, Johann Sellner, Per Soelberg Sorensen, Maria Pia Sormani, Jens Thomas Wuerfel, Mark S. Freedman
Summary: The MESEMS study aimed to evaluate the safety and activity of autologous bone marrow-derived MSCs in patients with active multiple sclerosis. The results showed that MSC treatment did not affect GELs and does not support its use in treating multiple sclerosis.
Article
Radiology, Nuclear Medicine & Medical Imaging
Sarah C. Scharm, Jens Vogel-Claussen, Cornelia Schaefer-Prokop, Sabine Dettmer, Lars Knudsen, Danny Jonigk, Jan Fuge, Rosa-Marie Apel, Tobias Welte, Frank Wacker, Antje Prasse, Hoen-oh Shin
Summary: The study found that CT-derived functional parameters of regional ventilation and parenchymal late enhancement may serve as early imaging markers for idiopathic pulmonary fibrosis progression.
EUROPEAN RADIOLOGY
(2021)
Article
Physiology
Yaniv Tomer, Jennifer Wambach, Lars Knudsen, Ming Zhao, Luis R. Rodriguez, Aditi Murthy, Frances White, Alessandro Venosa, Jeremy Katzen, Matthias Ochs, Aaron Hamvas, Michael F. Beers, Surafel Mulugeta
Summary: ABCA3(E292V) variant, a lipid transporter, plays a role in lung diseases by disrupting alveolar type 2 cell homeostasis, leading to parenchymal diseases.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Immunology
Marie T. Moog, Christopher Hinze, Tina Bormann, Franziska Aschenbrenner, Lars Knudsen, David S. DeLuca, Danny Jonigk, Lavinia Neubert, Tobias Welte, Jack Gauldie, Martin Kolb, Ulrich A. Maus
Summary: Research has found accumulations of B cells in patients with pulmonary fibrosis as well as in experimental animal models. However, comparative analysis between wild-type mice and B cell-deficient mice does not support a significant involvement of B cells in mouse lung fibrogenesis.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jessica Knoell, Shashi Chillappagari, Lars Knudsen, Martina Korfei, Ruth Dartsch, Danny Jonigk, Mark P. Kuehnel, Konrad Hoetzenecker, Andreas Guenther, Poornima Mahavadi
Summary: Endoplasmic reticulum (ER) and mitochondria (mito) play a vital role in alveolar type II cell (AEC2) homeostasis and are both stressed in idiopathic pulmonary fibrosis (IPF) patients. This study shows that ER-mitochondrial tethering is reduced in IPF AEC2 and experimental ER stress, partly due to decreased PACS2 protein levels. Treatment with the TRPV1-modulating drug capsaicin (CPS) restores PACS2 levels and reduces alveolar epithelial apoptosis, suggesting therapeutic potential in IPF.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Petra Khan, Julien Roux, Sabrina Blumer, Lars Knudsen, Danny Jonigk, Mark P. Kuehnel, Michael Tamm, Katrin E. Hostettler
Summary: In this study, it was found that cultured alveolar basal cells have the potential to differentiate into secretory epithelial-like cells and aberrant basaloid-like cells. However, due to the persistent expression of KRT5, a complete differentiation into aberrant basaloid cells was not achieved in the culture conditions. Importantly, differentiation seemed reversible by changing the cells microenvironment.
Article
Biotechnology & Applied Microbiology
Petra Khan, Kleanthis Fytianos, Sabrina Blumer, Julien Roux, Amiq Gazdhar, Spasenija Savic, Lars Knudsen, Danny Jonigk, Mark P. Kuehnel, Sofia Mykoniati, Michael Tamm, Thomas Geiser, Katrin E. Hostettler
Summary: This study confirmed the presence of basal-like cells in peripheral lung tissue of patients with idiopathic pulmonary fibrosis (IPF) and investigated their paracrine effects on fibroblasts. The findings suggest that basal-like cells may play a role in limiting aberrant fibroblast activation and differentiation in IPF through paracrine mechanisms.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Anatomy & Morphology
Henri Schulte, Andreas Schmiedl, Christian Muehlfeld, Lars Knudsen
Summary: This study compared the effects of active cadaver dissection and e-learning resources on the learning of anatomy by medical students. The results showed no differences between the two groups in motivation and interest in anatomy, but the non-dissection group had higher concerns about the Covid-19 pandemic. There were no differences in the scores of the knowledge test between the two groups before and after the educational intervention, but after the course, the dissection group demonstrated higher confidence levels and better scores in image-based questions. Students who frequently used online quizzes scored higher in the knowledge test. The study suggests that active dissection should be reintroduced in anatomical curricula in the post-pandemic era.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2022)
Article
Pharmacology & Pharmacy
Kleanthis Fytianos, Ronja Schliep, Sofia Mykoniati, Petra Khan, Katrin E. Hostettler, Michael Tamm, Amiq Gazdhar, Lars Knudsen, Thomas Geiser
Summary: The overexpression of SDF-1 beta can reduce collagen deposition, improve lung histology, and induce apoptosis of myofibroblasts. Additionally, it increases alveolar epithelial cell numbers and proliferation, showing potential as a new approach for the treatment of lung fibrosis.
Article
Multidisciplinary Sciences
Arvind Konkimalla, Satoshi Konishi, Yoshihiko Kobayashi, Preetish Kadur Lakshminarasimha Murthy, Lauren Macadlo, Ananya Mukherjee, Zachary Elmore, So-Jin Kim, Ann Marie Pendergast, Patty J. Lee, Aravind Asokan, Lars Knudsen, Jose Javier Bravo-Cordero, Aleksandra Tata, Purushothama Rao Tata
Summary: Epithelial cells have a single apical domain, and alveolar type-2 epithelial cells (AT2s) can have multiple apical domains that support multiple alveoli. AT2s dynamically reorganize multiple apical domains during development and injury repair. Dysregulation of multiple apical domains can occur with cigarette smoke and oncogene activation.
Article
Physiology
Alex M. Reimelt, Dragos M. Vasilescu, Richard Beare, Jonas Labode, Lars Knudsen, Roman Grothausmann
Summary: This study investigates the alveolar morphomics in different lobes of human lungs and finds that the alveoli in the upper lobes have larger volumes and surface areas compared to the lower lobes, and there are fewer connections between alveoli in the upper lobes. This approach provides baseline measures for in-depth studies of mechanical conditions and morphology.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Alison M. Wallbank, Alyssa E. Vaughn, Steve Niemiec, Jill Bilodeaux, Tanner Lehmann, Lars Knudsen, Elayaraja Kolanthai, Sudipta Seal, Carlos Zgheib, Eva Nozik, Kenneth W. Liechty, Bradford J. Smith
Summary: Acute respiratory distress syndrome (ARDS) has a high mortality rate (around 40%) and often requires mechanical ventilation. The inflamed and injured lung is susceptible to ventilator-induced lung injury (VILI). This study investigated whether treatment with CNP-miR146a, a conjugate of novel free radical scavenging cerium oxide nanoparticles (CNP) to the anti-inflammatory microRNA (miR)-146a, could protect murine lungs from acute lung injury (ALI) induced by endotoxin and subsequent VILI. The results showed that CNP-miR146a reduced the severity of ALI and slowed down the progression of VILI, indicating its potential as a therapeutic approach.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2023)
Review
Physiology
Lars Knudsen, Benjamin Hummel, Christoph Wrede, Richard Zimmermann, Carrie E. Perlman, Bradford J. Smith
Summary: Within the pulmonary acini, ventilation and blood perfusion occur on a large surface area, separated by a thin blood-gas barrier, allowing efficient gas exchange. Lung diseases can lead to abnormalities in micromechanics, which can cause damage to the blood-gas barrier. The mechanisms of adaptation to increased alveolar volume include unfolding, stretching, and shape changes. Techniques such as imaging and stereology can be used to study these processes. However, real-time visualization is currently not possible.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Physiology
Jill Bilodeaux, Huda Farooqi, Maria Osovskaya, Alexander Sosa, Alison Wallbank, Lars Knudsen, Peter D. Sottile, David J. Albers, Bradford J. Smith
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Sarah C. Scharm, Cornelia Schaefer-Prokop, Moritz Willmann, Jens Vogel-Claussen, Lars Knudsen, Danny Jonigk, Jan Fuge, Tobias Welte, Frank Wacker, Antje Prasse, Hoen-oh Shin
Summary: This study aimed to investigate the correlation between increased ventilation at baseline CT and morphological changes suggestive of fibrosis in follow-up CT. The results showed significant density increases and volume loss in areas of severely increased ventilation, particularly in high attenuation areas.
EUROPEAN RADIOLOGY
(2022)
Article
Computer Science, Information Systems
Oleg Lobachev, Takuya Funatomi, Alexander Pfaffenroth, Reinhold Foerster, Lars Knudsen, Christoph Wrede, Michael Guthe, David Haberthur, Ruslan Hlushchuk, Thomas Salaets, Jaan Toelen, Simone Gaffling, Christian Muehlfeld, Roman Grothausmann
Summary: This work proposes a ground-truth-based evaluation method for registration, generating test data and evaluating registration methods with known ground truths. Evaluation across six different registration methods on distorted 3D data sets of animal lungs reveals both under- and over-registration.