期刊
HELICOBACTER
卷 21, 期 6, 页码 504-522出版社
WILEY-BLACKWELL
DOI: 10.1111/hel.12307
关键词
Helicobacter heilmannii; adhesion; Hof outer membrane protein; MUC13
资金
- Research Foundation Flanders (FWO Vlaanderen)
- Research Fund of Ghent University [01SC0312]
- China Scholarship Counsel (CSC) [2011691031]
- Swedish Research council [521-2011-2370]
- Swedish Research council Formas [221-2011-1036, 221-2013-590]
- Swedish Cancer Society
BackgroundHelicobacter heilmannii is a zoonotic bacterium associated with gastric disease in humans. We recently showed that H. heilmannii binds to human gastric mucins and epithelial cells and highlighted a potential role for the murine Muc13 mucin in gastric Helicobacter colonization. The aims of this study were to investigate the role of the H. heilmannii hof gene locus encoding HofH/F/E/G/C/D in adhesion to the gastric mucosa and induction of increased gastric Muc13 expression. MethodsBacterial hof gene and host gene expression experiments, Helicobacter binding assays and experimental infection studies in mice were performed. H. pylori and its hofF mutant were included for comparison. ResultsHelicobacter heilmannii strains lacking HofE or HofF showed a clear decrease in binding to gastric mucins and epithelial cells as well as a lower gastric colonization level in the stomach of Balb/c mice at 4 and 9 weeks post-infection compared to the H. heilmannii wildtype strain. Interestingly, H. heilmannii hofE and hofF and H. pylori hofF did not induce an increased expression of MUC13 in human gastric epithelial cells and of Muc13 in the stomach of mice. Finally, we demonstrated that IL-1 is induced in the stomach as a response to Helicobacter colonization which on its turn is involved in the expression of MUC13/Muc13 in the gastric epithelium. ConclusionThese novel results in Helicobacter research identified H. heilmannii HofE and HofF as adhesins and suggest an important role of H. heilmannii HofE and HofF and H. pylori HofF in IL-1-induced gastric MUC13/Muc13 expression.
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