Single nucleus RNA-sequencing: how it's done, applications and limitations

Adipocyte Microenvironment: Everybody in the Neighborhood Talks about the Temperature

(2021) Farnaz Shamsi et al.   Cell Metabolism

Differential regulation of β-catenin-mediated transcription via N- and C-terminal co-factors governs identity of murine intestinal epithelial stem cells

(2021) Costanza Borrelli et al.   Nature Communications

Molecular characterization of the human kidney interstitium in health and disease

(2021) Daria Barwinska et al.   Science Advances

A robust method of nuclei isolation for single-cell RNA sequencing of solid tissues from the plant genus Populus

(2021) Daniel Conde et al.   PLoS One

Single cell transcriptional and chromatin accessibility profiling redefine cellular heterogeneity in the adult human kidney

(2021) Yoshiharu Muto et al.   Nature Communications

A flexible microfluidic system for single-cell transcriptome profiling elucidates phased transcriptional regulators of cell cycle

(2021) Karen Davey et al.   Scientific Reports

Adipocyte heterogeneity underlying adipose tissue functions

(2021) Zinger Yang Loureiro et al.   ENDOCRINOLOGY

Single-Nucleus RNA Sequencing Identifies New Classes of Proximal Tubular Epithelial Cells in Kidney Fibrosis

(2021) Yueh-An Lu et al.   JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY

Cross-Species analysis defines the conservation of anatomically-segregated VMH neuron populations

(2021) Alison H Affinati et al.   eLife

Histone H3K27me3 demethylases regulate human Th17 cell development and effector functions by impacting on metabolism

(2020) Adam P. Cribbs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single cell and genetic analyses reveal conserved populations and signaling mechanisms of gastrointestinal stromal niches

(2020) Ji-Eun Kim et al.   Nature Communications

Systematic comparison of single-cell and single-nucleus RNA-sequencing methods

(2020) Jiarui Ding et al.   NATURE BIOTECHNOLOGY

A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors

(2020) Michal Slyper et al.   NATURE MEDICINE

Single-nucleus RNA-seq and FISH identify coordinated transcriptional activity in mammalian myofibers

(2020) Matthieu Dos Santos et al.   Nature Communications

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

(2020) Ashwin Narayanan et al.   Jove-Journal of Visualized Experiments

Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers

(2020) Michael J. Petrany et al.   Nature Communications

The single-cell transcriptomic landscape of early human diabetic nephropathy

(2019) Parker C. Wilson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-nucleus transcriptomic survey of cell diversity and functional maturation in postnatal mammalian hearts

(2018) Peng Hu et al.   GENES & DEVELOPMENT

Advantages of Single-Nucleus over Single-Cell RNA Sequencing of Adult Kidney: Rare Cell Types and Novel Cell States Revealed in Fibrosis

(2018) Haojia Wu et al.   JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY

Single-nucleus and single-cell transcriptomes compared in matched cortical cell types

(2018) Trygve E. Bakken et al.   PLoS One

Massively parallel single-nucleus RNA-seq with DroNc-seq

(2017) Naomi Habib et al.   NATURE METHODS

Massively parallel digital transcriptional profiling of single cells

(2017) Grace X. Y. Zheng et al.   Nature Communications

Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells

(2015) Allon M. Klein et al.   CELL

Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

(2015) Evan Z. Macosko et al.   CELL