期刊
BIOACTIVE MATERIALS
卷 9, 期 -, 页码 523-540出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2021.07.022
关键词
Keratinocytes; Skin tissue engineering; Reversible immortalization; SV40 large T antigen; PPCN; Skin wound healing
资金
- Chongqing Support Program for Entrepreneurship and Innovation [cx2019113]
- Science and Technology Research Plan Project of Chongqing Education Commission [KJQN201900410]
- Youth Innovative Talent Training Program of Chongqing Education Commission [CY200409]
- National Key Research and Development Program of China [2016YFC1000803]
- National Institutes of Health [DE030480]
- Medical Scientist Training Program of the National Institutes of Health [T32 GM007281]
- University of Chicago Cancer Center Support Grant [P30CA014599]
- National Center for Advancing Translational Sciences of the National Institutes of Health [UL1 TR000430]
- Mabel Green Myers Research Endowment Fund
- University of Chicago Orthopaedics Alumni Fund
- University of Chicago SHOCK Fund
- Funding for Post-doctoral Research (Chongqing Human Resources and Social Security Bureau) [298]
Skin injury is repaired through a multi-phase wound healing process. Effective management of large chronic skin wounds remains a clinical challenge. Exogenous keratinocytes, in combination with a citrate-based scaffold, have been found to enhance skin wound healing.
Skin injury is repaired through a multi-phase wound healing process of tissue granulation and re-epithelialization. Any failure in the healing process may lead to chronic non-healing wounds or abnormal scar formation. Although significant progress has been made in developing novel scaffolds and/or cell-based therapeutic strategies to promote wound healing, effective management of large chronic skin wounds remains a clinical challenge. Keratinocytes are critical to re-epithelialization and wound healing. Here, we investigated whether exogenous keratinocytes, in combination with a citrate-based scaffold, enhanced skin wound healing. We first established reversibly immortalized mouse keratinocytes (iKera), and confirmed that the iKera cells expressed keratinocyte markers, and were responsive to UVB treatment, and were non-tumorigenic. In a proof-of-principle experiment, we demonstrated that iKera cells embedded in citrate-based scaffold PPCN provided more effective re-epithelialization and cutaneous wound healing than that of either PPCN or iKera cells alone, in a mouse skin wound model. Thus, these results demonstrate that iKera cells may serve as a valuable skin epithelial source when, combining with appropriate biocompatible scaffolds, to investigate cutaneous wound healing and skin regeneration.
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