期刊
PLANTS-BASEL
卷 10, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/plants10091861
关键词
Ficus carica L.; salinity stress; carbohydrates metabolism; RT-qPCR; qNMR; H-1 NMR
资金
- University of Pisa, Italy
- European Union through a national MIUR (Italy) [FIGGEN/PRIMA19_00197]
- METROFOOD-CZ research infra-structure project (MEYS) [LM2018100]
Under salt stress, the transcription levels of carbohydrate metabolism-related genes in ripe fig fruits generally increased, but there was no significant change in glucose and fructose content, while the increases in sucrose and D-sorbitol contents may be attributed to the up-regulation of gene transcription.
Fig trees (Ficus carica L.) are commonly grown in the Mediterranean area, where salinity is an increasing problem in coastal areas. Young, fruiting plants of cv. Dottato were subjected to moderate salt stress (100 mM NaCl added to irrigation water) for 48 days before fruit sampling. To clarify the effect of salinity stress, we investigated changes in the transcription of the main sugar metabolism-related genes involved in the synthesis, accumulation and transport of soluble carbohydrates in ripe fruits by quantitative real-time PCR as well as the content of soluble sugars by quantitative H-1 nuclear magnetic resonance spectroscopy. A general increase in the transcript levels of genes involved in the transport of soluble carbohydrates was observed. Alkaline-neutral and Acid Invertases transcripts, related to the synthesis of glucose and fructose, were up-regulated in ripe fruits of NaCl-stressed plants without a change in the content of D-glucose and D-fructose. The increases in sucrose and D-sorbitol contents were likely the result of the up-regulation of the transcription of Sucrose-Synthase- and Sorbitol-Dehydrogenase-encoding genes.
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