Article
Biochemistry & Molecular Biology
Miaomiao Yang, Qiang Fan, Tom K. Hei, Guodong Chen, Wei Cao, Gang Meng, Wei Han
Summary: In this study, we used scRNA-seq to reveal the changes in cell subtype abundance in RP lung tissue of mice and identified markers and transcriptional states associated with inflammatory response and fibrosis formation in RP lung tissue.
Article
Medicine, Research & Experimental
Mirko Signorelli, Mitra Ebrahimpoor, Olga Veth, Kristina Hettne, Nisha Verwey, Raquel Garcia-Rodriguez, Christa L. Tanganyika-deWinter, Luz B. Lopez Hernandez, Rosa Escobar Cedillo, Benjamin Gomez Diaz, Olafur T. Magnusson, Hailiang Mei, Roula Tsonaka, Annemieke Aartsma-Rus, Pietro Spitali
Summary: This study utilized RNA-sequencing to investigate pathophysiological changes in skeletal muscle of dystrophic mouse models, demonstrating how these changes are reflected in blood samples. By analyzing gene expression in muscle and blood, the study identified genes associated with safety and efficacy of antisense drugs for DMD treatment. The results suggest that blood RNA-sequencing can be a valuable tool in evaluating disease progression in dystrophic mice and patients.
EMBO MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yanru Xing, Xi Yang, Haixiao Chen, Sujun Zhu, Jinjin Xu, Yuan Chen, Juan Zeng, Fang Chen, Mark Richard Johnson, Hui Jiang, Wen-Jing Wang
Summary: Temporary storage conditions significantly impact the gene expression profiles of leukocytes, with storage at room temperature for 24 hours leading to changes in a large number of genes related to processes such as apoptotic signaling pathway and lymphocyte activation. However, storing blood samples at 4 degrees Celsius within 6 hours largely maintains their original transcriptome.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Pharmacology & Pharmacy
Bing Li, Ge Ren, Wei Guo, Jiang Zhang, Sai-Kit Lam, Xiaoli Zheng, Xinzhi Teng, Yunhan Wang, Yang Yang, Qinfu Dan, Lingguang Meng, Zongrui Ma, Chen Cheng, Hongyan Tao, Hongchang Lei, Jing Cai, Hong Ge
Summary: This study investigates the impact of lung function on radiation pneumonitis prediction using a dual-omics analysis method, and finds that dual-omics features from different lung functional regions can improve the prediction of radiation pneumonitis for lung cancer patients.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Xu Shi, Xiao Wang, Andrew F. Neuwald, Leena Halakivi-Clarke, Robert Clarke, Jianhua Xuan
Summary: BayesDenovo addresses the challenge of de novo transcriptome assembly by accurately reconstructing splicing graphs using a read-guided and Bayesian strategy, achieving higher accuracy and reducing false positives, especially for alternatively spliced genes and highly/lowly expressed transcripts. Additionally, it is more robust on multiple replicates and can identify phenotype-specific transcripts associated with breast cancer recurrence.
SCIENTIFIC REPORTS
(2021)
Article
Respiratory System
Evans R. Fernandez Perez, Laura D. Harmacek, Brian P. O'Connor, Thomas Danhorn, Brian Vestal, Lisa A. Maier, Tilman L. Koelsch, Sonia M. Leach
Summary: PBMC expression profiles have prognostic value in patients with CHP, helping distinguish between those with disease progression and those without. Classification models based on gene expression and clinical factors outperform models based solely on clinical factors.
Article
Genetics & Heredity
Juber Herrera-Uribe, Jayne E. Wiarda, Sathesh K. Sivasankaran, Lance Daharsh, Haibo Liu, Kristen A. Byrne, Timothy P. L. Smith, Joan K. Lunney, Crystal L. Loving, Christopher K. Tuggle
Summary: The study conducted transcriptome analysis of pig peripheral blood immune cells at both bulk and single-cell levels, identifying different cell types and their associated gene expression features. The research demonstrated significant correlations in gene expression between pig cells and human PBMC data, providing insights into the similarities between pig and human immune systems.
FRONTIERS IN GENETICS
(2021)
Article
Genetics & Heredity
Hui-Qi Qu, Charlly Kao, James Garifallou, Fengxiang Wang, James Snyder, Diana J. Slater, Cuiping Hou, Michael March, John J. Connolly, Joseph T. Glessner, Hakon Hakonarson
Summary: This study investigated the impact of varying time periods from blood draw to PBMC isolation on the subsequent transcriptome profiling of different cell types in PBMCs using single cell RNA-seq. The results showed significant changes in the transcriptome expression of various cell types over time, highlighting the importance of considering this factor in interpreting experimental results.
Article
Multidisciplinary Sciences
August Guang, Mark Howison, Felipe Zapata, Charles Lawrence, Casey W. Dunn
Summary: treeinform is a method that utilizes phylogenetic information across species to improve transcriptome assemblies within species. It helps identify and correct the error of mistaking different transcripts of the same gene as transcripts from multiple closely related genes.
Article
Biochemical Research Methods
Qianqian Zhang, Xing Xu, Li Lin, Jian Yang, Xing Na, Xin Chen, Lingling Wu, Jia Song, Chaoyong Yang
Summary: Cilo-seq is a high-performance platform for single-cell RNA sequencing library construction. It utilizes digital microfluidics in a single device, enabling convenient single-cell isolation, efficient nucleic acid amplification, low-loss nucleic acid purification, and high-quality library preparation.
Article
Multidisciplinary Sciences
Chaoqiong Ding, Xiang Yan, Mengying Xu, Ran Zhou, Yuancun Zhao, Dan Zhang, Zongyao Huang, Zhenzhong Pan, Peng Xiao, Huifang Li, Lu Chen, Yuan Wang
Summary: This study reports the full-length transcriptome of mouse neural stem cells (NSCs) at different developmental stages, providing an important resource for studying NSC fate determination, neural development, and disease in the neuroscience community.
Article
Entomology
Erwan Poivet, Aurore Gallot, Nicolas Montagne, Pavel Senin, Christelle Monsempes, Fabrice Legeai, Emmanuelle Jacquin-Joly
Summary: Starvation enhances olfactory sensitivity in animals, including insects, to help them search for food. This study sequenced and compared the transcriptomes of olfactory organs in caterpillars under fed and starved conditions, revealing regulation of various biological processes including glucose metabolism, immune defense, foraging activity, and olfaction upon starvation. The study identified changes in transcripts encoding chemosensory proteins and odorant-degrading enzymes that play a role in the dynamics and sensitivity of olfactory responses.
Article
Oncology
Bing Li, Xiaoli Zheng, Jiang Zhang, Saikit Lam, Wei Guo, Yunhan Wang, Sunan Cui, Xinzhi Teng, Yuanpeng Zhang, Zongrui Ma, Ta Zhou, Zhaoyang Lou, Lingguang Meng, Hong Ge, Jing Cai
Summary: The study evaluates the effectiveness of features obtained from proposed incremental-dose-interval-based lung subregion segmentation for predicting grade >= 2 acute radiation pneumonitis (ARP) in lung cancer patients. Results show that features from lung subregions outperform those from the whole lung in predicting ARP, especially when using dosiomics or combined radiomics-dosiomics features. This improved model performance could enhance clinical management and patient quality of life.
Review
Immunology
Min Wang, Shuhui Xu, Hui Zhu
Summary: This report presents a rare case of double pneumonitis (RP and RRP) in a patient with non-small lung cancer (NSCLC), with RP occurring at 3 months and RRP induced by anti-PD-1 sintilimab at 10 months after radiotherapy. This case draws attention to the identification of immune or radiation pneumonitis, its potential mechanism, and the treatment strategy for RRP.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Di Wu
Summary: The germinal vesicle (GV) stage is a critical transition point in mammalian oocyte development that determines oocyte quality through active RNA degradation and transcriptome reprofiling. Oocyte RNA-seq has revealed differences between non-surrounded nucleolus (NSN) and surrounded nucleolus (SN) phases. Single oocyte RNA-seq (soRNA-seq) shows higher reproducibility and a better illustration of gene changes during oocyte development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Jin Zhou, Zheming Liu, Huibo Zhang, Tianyu Lei, Jiahui Liu, Yaqi Zhao, Yi Yao, Qibin Song
Summary: This study explores the value of BACH1 in predicting the overall survival of early-stage lung adenocarcinoma. It establishes a BACH1 expression-based prognostic model that shows good predictive power and clinical applicability. Higher expression of BACH1 is associated with worse overall survival in patients with lung adenocarcinoma.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Oncology
Yujia Li, Hui Chen, Qi Yang, Lixin Wan, Jing Zhao, Yuanyuan Wu, Jiaxin Wang, Yating Yang, Menglan Niu, Hongliang Liu, Junqi Liu, Hushan Yang, Shaogui Wan, Yanming Wang, Dengke Bao
Summary: Our study reveals that Drp1 overexpression induces mitochondrial dysfunction and cytosolic mtDNA stress, thereby activating the cGAS-STING pathway, promoting autophagy, and enhancing ESCC progression. The findings suggest a potential mechanism for targeting Drp1 in cancer therapy.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Biochemical Research Methods
Michael J. Harris, Hao Chen, Tianyu Cai, Yuting Yi, Qaowen Deng, Yi Yao, Tianle Lan, Yanfeng Guo, Xiufang Xu, Xian Wen, Joshua E. McGee, Daniella Tatang, James Brock, Feng Shi, Li Zhou
Summary: The study demonstrates a synthetic E3 fusion protein called ReceptorTAC, which can regulate gene expression by targeting T cell antigen receptor degradation, achieving specific degradation of TCR.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Oncology
Zheming Liu, Jing Wang, Fuben Liao, Qibin Song, Yi Yao
Summary: Platelets are small anucleate cells that circulate in the blood and form thrombi. Tumor-educated platelets derived from cancer patients play a role in cancer-associated thrombosis. Studies show that tumor-educated platelets from melanoma-burdened mouse models demonstrate enhanced migration and clot formation, but are inhibited by plasma from tumor mice. RNA sequence profile of tumor-educated platelets reveals upregulation of genes associated with cell migration and cytoskeleton, shedding light on the process of thrombus formation.
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Haohan Zhang, Yi Yao, Jie Wu, Jin Zhou, Chen Zhao, Junju He, Bin Xu
Summary: By analyzing the scores and clustering of various cells in the tumor microenvironment of hepatocellular carcinoma, this study identified four subtypes with different biological characteristics and potential relevance.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Hao Gu, Ruixia Song, Narasimha M. Beeraka, Tingxuan Li, Di Zhao, Junqi Liu, Ruitai Fan
Summary: The objective of this study was to construct a nomogram for predicting the overall survival (OS) of patients with primary pulmonary sarcoma (PPS). The nomogram was based on stage, lymph node dissection, tumor size, degree of differentiation, and therapies. The nomogram showed a strong predictive ability with high accuracy for the prognosis of PPS patients.
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
(2023)
Article
Oncology
Songjiang He, Shi Zhang, Yi Yao, Bin Xu, Zhili Niu, Fuben Liao, Jie Wu, Qibin Song, Minglun Li, Zheming Liu
Summary: Glutamine metabolism is significant in the clinical outcomes of patients with pan-cancer and is closely associated with several hallmarks of a malignant tumor.
FRONTIERS IN ONCOLOGY
(2022)
Article
Pharmacology & Pharmacy
Yingge Li, Jie Chen, Bin Wang, Ziwen Xu, Ci Wu, Junfeng Ma, Qibin Song, Qing Geng, Jinming Yu, Huadong Pei, Yi Yao
Summary: This study aims to investigate the regulatory mechanisms of nucleotide de novo synthesis and drug resistance. By analyzing ChIP-Seq data, RNA sequencing, and luciferase-based promoter assay, the researchers identified FOXK2 as a key regulator of nucleotide de novo synthesis. FOXK2 directly regulates the expression of nucleotide synthetic genes, promoting tumor growth and cancer cell resistance to chemotherapy. In clinical analysis, elevated expression of FOXK2 is associated with increased nucleotide synthetic gene expression and poor prognosis in hepatocellular carcinoma patients.
DRUG RESISTANCE UPDATES
(2023)
Article
Oncology
Di Zhao, Xiaoyan Wang, Narasimha M. Beeraka, Runze Zhou, Haohao Zhang, Yanxia Liu, Yinghui Zhang, Ying Zhang, Guijun Qin, Junqi Liu
Summary: This study aimed to elucidate the association between high body mass index (BMI) and pathological findings in breast cancer patients. The results showed that for women under the age of 55, high BMI was associated with lower breast cancer incidence (OR = 0.313, CI: 0.240 -0.407), while in women over the age of 55, it was associated with histological grade lower than 2 (OR = 0.288, CI: 0.152 -0.544).
WORLD JOURNAL OF ONCOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Shuting Xiao, Yi Yao, Shuilin Liao, Bin Xu, Xue Li, Yuxiao Zhang, Lei Zhang, Qiang Chen, Haoneng Tang, Qibin Song, Ming Dong
Summary: Researchers have developed an EV-MPDS system based on FRET signals, which allows convenient diagnosis of lung cancer without the need for EV extraction and purification. In clinical samples, this system demonstrated improved accuracy and sensitivity compared to the ELISA detection method, with early screening accuracy further enhanced through machine learning analysis of five biomarkers.
Article
Oncology
Zheming Liu, Songjiang He, Zhou Huang, Jiahui Liu, Yiping Gong, Yi Yao, Xue Zhang
Summary: Drug resistance is a major challenge in immunotherapy for triple negative breast cancer patients. This study investigated the mechanisms of drug resistance using single-cell RNA sequencing data and TCGA transcriptomic data. CD8+ NKT cells and classical monocytes were found to play vital roles in the immunotherapy response, and DUSP1 was identified as a potential key factor in immunotherapy resistance.
Review
Oncology
Yingmei Wen, Yi Dong, Lina Yi, Guifang Yang, Mengxia Xiao, Qingqing Li, Chen Zhao, Dafu Ye, Yi Yao
Summary: This study reports the treatment course of a patient with advanced pulmonary sarcomatoid carcinoma. The patient initially received treatment with anlotinib, followed by the addition of Pembrolizumab, leading to partial response. The patient survived for 14 months with no significant adverse reactions during treatment. Genomic analysis revealed mutations associated with treatment efficacy.
FRONTIERS IN ONCOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Jie Chen, Siqi Yang, Yingge Li, Xu Ziwen, Pingfeng Zhang, Qibin Song, Yi Yao, Huadong Pei
Summary: The de novo nucleotide biosynthetic pathway is a highly conserved and crucial biochemical pathway found in almost all organisms. Dysregulation of this pathway has been linked to the development of many human diseases, including cancer. In this review, the authors provide a comprehensive overview of the pathway, its regulatory mechanisms, its role in tumorigenesis, and potential targeting opportunities.
Meeting Abstract
Oncology
Bin Xu, Chen Zhao, Hao Zhong, Tianyu Lei, Huibo Zhang, Jie Wu, Lan Li, Yi Yao, Qibin Song
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Meeting Abstract
Oncology
Yi Yao, Jin Zhou, Haohan Zhang, Jie Wu, Yaqi Zhao, Zheming Liu, Bin Xu, Qibin Song
JOURNAL OF CLINICAL ONCOLOGY
(2022)