Article
Biochemistry & Molecular Biology
Felisa Rey, Paulo Cartaxana, Susana Aveiro, Michael Greenacre, Tania Melo, Pedro Domingues, M. Rosario Domingues, Sonia Cruz
Summary: A study on the sea slug Elysia timida revealed that light intensity influences the degradation of stolen chloroplasts (kleptoplasts), with older kleptoplasts being targeted for degradation. The lipidome of E. timida showed differences under different light treatments, indicating a light-driven remodelling of the lipidome.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2023)
Article
Nutrition & Dietetics
Bret M. Rust, Matthew J. Picklo, Lin Yan, Aaron A. Mehus, Huawei Zeng
Summary: Time-restricted feeding is an effective method to reduce the risk of obesity-related metabolic diseases by altering nutrient absorption, fecal microbiome, and lipidome. The study found that different feeding timing and fat content can affect fecal fatty acid levels and the composition of microbiota.
Article
Cell Biology
Carla Ferreri, Anna Sansone, Marios G. Krokidis, Annalisa Masi, Barbara Pascucci, Mariarosaria D'Errico, Chryssostomos Chatgilialoglu
Summary: Oxygen plays a crucial role in lipid metabolism, especially in cells with impaired repair mechanisms. This study examined the effects of different oxygen tensions on fatty acid-based membrane remodeling in two cell models. The results showed that oxygen tension influences enzymatic activity and fatty acid composition, suggesting a relationship between oxygen and cellular functions.
Article
Immunology
Michela Cerone, Matthew Roberts, Terry K. Smith
Summary: Crithidia fasiculata is a parasitic protozoan closely related to other kinetoplastid parasites. Studying kinetoplastid parasites provides unique insights into alternative mechanisms to traditional eukaryotic metabolic pathways. Crithidia is a monogenetic parasite and can be used as a model organism for kinetoplastid research. Little is known about Crithidia, but similarities to other kinetoplast species have been shown in terms of energy metabolism and genetics. Additionally, Crithidia can be used as a eukaryotic expression system for expressing proteins from other kinetoplastids and other eukaryotes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Elisabetta Del Duca, Anna Sansone, Mayla Sgrulletti, Federica Di Nolfo, Loredana Chini, Carla Ferreri, Viviana Moschese
Summary: Peanut allergy is a lifelong and potentially life-threatening disease that affects families and communities. A preliminary study explored the fatty-acid-based membrane lipidome profile of patients with angioedema and/or anaphylaxis after peanut ingestion. The study found significant differences in the levels of omega-6 and omega-3 fatty acids between allergic patients and healthy individuals, as well as a correlation between specific immunoglobulin E (IgE) levels and certain fatty acids. The results of this study suggest the need for larger screenings and further research to understand the molecular mechanisms and develop effective treatments for chronic allergic disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Daniel Hornburg, Si Wu, Mahdi Moqri, Xin Zhou, Kevin Contrepois, Nasim Bararpour, Gavin M. Traber, Baolong Su, Ahmed A. Metwally, Monica Avina, Wenyu Zhou, Jessalyn M. Ubellacker, Tejaswini Mishra, Sophia Miryam Schussler-Fiorenza Rose, Paula B. Kavathas, Kevin J. Williams, Michael P. Snyder
Summary: This study reports over 800 lipid species and their roles in health-to-disease transitions, such as in diabetes, ageing, and inflammation. Longitudinal lipidomic profiling of plasma samples from 112 participants revealed dynamic changes in the plasma lipidome during respiratory viral infection, insulin resistance, and ageing, highlighting the potential roles of lipids in immune homoeostasis and inflammation regulation.
Article
Cardiac & Cardiovascular Systems
Brian V. Hong, Christopher H. Rhodes, Joanne K. Agus, Xinyu Tang, Chenghao Zhu, Jack Jingyuan Zheng, Angela M. Zivkovic
Summary: This study found that prolonged fasting significantly alters the composition of plasma lipids and concentrations of several lipid species. These changes may serve as sensitive biomarkers of prolonged fasting and have important implications for the management of cardiometabolic health and healthy aging. Further research and validation in larger cohorts and different population groups are warranted.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Plant Sciences
Antonio Pannico, Giuseppe Carlo Modarelli, Silvia Rita Stazi, Matteo Giaccone, Raffaele Romano, Youssef Rouphael, Chiara Cirillo
Summary: Foliar nutrition is used globally to address microelement deficiencies and optimize yield performances, but it can also positively impact hazelnut nut quality and kernel composition. Recent studies emphasize the importance of managing not only micronutrients, but also major components like nitrogen for sustainable orchard nutrition. In our research, various foliar fertilizers were tested to understand their effectiveness in supporting hazelnut productivity and improving nut and kernel quality. The results showed that foliar fertilization influenced tree growth, increased kernel weight, and reduced blank incidence compared to the control. Treatment differences were also observed in fat, protein, and carbohydrate concentration, with fertilized treatments showing higher fat concentrations and total polyphenol content. The composition of oil in the kernels was also improved through foliar fertilization, although the response of fatty acid composition to nutrient spray varied. Oleic acid concentration increased while palmitic acid concentration decreased in fertilized plants. Additionally, CD and B trees exhibited a higher ratio of unsaturated/saturated fatty acids compared to untreated trees. Finally, foliar spraying improved lipid stability by increasing total polyphenol concentration.
Article
Agriculture, Multidisciplinary
Yang Jin, Thomas Nelson Harvey, Zdenka Bartosova, Sahar Hassani, Per Bruheim, Simen Rod Sandve, Jon Olav Vik
Summary: The study showed that salmon living in seawater have a higher demand for DHA, with faster changes in lipid composition in the gut and higher absorption and transport rates.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Nutrition & Dietetics
Joaquim Calvo-Lerma, Marta Selma-Royo, David Hervas, Baoru Yang, Linda Intonen, Sonia Gonzalez, Cecilia Martinez-Costa, Kaisa M. Linderborg, Maria Carmen Collado
Summary: This study explored the correlation between the fatty acid composition in breast milk and infant growth. The findings suggest that maternal intake of nuts, dietary sources of zinc, iron, and B group vitamins may predict a high-unsaturated fatty acid profile in breast milk, which is associated with infant growth in the first year of life.
FRONTIERS IN NUTRITION
(2022)
Article
Food Science & Technology
Guodong Rao, Jianguo Zhang, Xiaoxia Liu, Xue Li, Chenhe Wang
Summary: The study revealed the variation in fatty acids and minor components in olive fruits of different colors, providing insights into the determination of optimal harvest strategies for olive oil production. Additionally, genes related to the biosynthetic pathways of hydroxytyrosol and oleuropein were identified through the combined analysis of gene expression data.
Article
Plant Sciences
Maria Oliviero, Antonio Luca Langellotti, Giovanni L. Russo, Marco Baselice, Andrea Donadio, Alberto Ritieni, Giulia Graziani, Paolo Masi
Summary: This study investigated the potential use of alternative organic carbon sources for the cultivation of Cynara cardunculus cells, and studied the effects of different carbon sources on the production of bioactive molecules.
Article
Biotechnology & Applied Microbiology
Giseli S. Rocha, Christopher C. Parrish, Evaldo L. G. Espindola
Summary: The study demonstrates that excess copper affects algal growth, photosynthetic efficiency, and lipid/fatty acid production, while not impacting chlorophyll levels. Copper presence leads to decreases in photosynthetic parameters and damage to photoprotective mechanisms.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Chemistry, Multidisciplinary
Ovidiu Procopet, Mircea Oroian
Summary: This study investigated the optimal conditions for extracting polyphenols from amaranth seed using a Box-Benhken design. The results showed that the solid-to-liquid ratio, methanol concentration, and ultra-turrax speed had significant effects on the extraction of polyphenols. Additionally, it was found that amaranth seed is a rich source of fatty acids and amino acids.
APPLIED SCIENCES-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Joao P. Monteiro, Elisabete Costa, Tania Melo, Pedro Domingues, Antoine Fort, M. Rosario Domingues, Ronan Sulpice
Summary: This study analyzed the lipid profile of three species of foliose Ulva grown in the same conditions and found important differences in fatty acid composition and sulfolipids. The study suggests that U. australis and U. rigida have higher nutritional value, while U. lacinulata has a lower n-6/n-3 ratio. Additionally, Ulva rigida contains sulfolipid species with potential bioactivity.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Chemistry, Medicinal
Philipp Doppler, Ricarda Kriechbaum, Maria Kaefer, Julian Kopp, Daniel Remias, Oliver Spadiut
Summary: A novel strain of Coelastrella terrestris was collected from red mucilage in a glacier foreland in Iceland. The addition of acetate increased the biomass productivity, indicating a promising approach for adonixanthin production.
Article
Plant Sciences
Charlotte Permann, Mattia Pierangelini, Daniel Remias, Louise A. Lewis, Andreas Holzinger
Summary: This study compares the photophysiological performance and phenolic content of two isolates of Zygnema from subpolar and polar regions. The results show that Zygnema strains cannot adapt to elevated temperatures.
Editorial Material
Plant Sciences
Eric Marechal, Linda Nedbalova
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Pavel Pribyl, Lenka Prochazkova
Summary: A new freshwater microalgal isolate, CCALA 1135, was characterized using a polyphasic approach (morphology, ultrastructure, fatty acid composition, 18S rRNA gene and rbcL analyses). The strain represents a new member of the Eustigmatophyceae, with unique mode of reproduction and high proportion of polyunsaturated fatty acids. It is proposed as a new species and new genus. This study contributes to the taxonomy of the Eustigmatophyceae.
EUROPEAN JOURNAL OF PHYCOLOGY
(2023)
Article
Limnology
Jan Fott, Linda Nedbalova, Marek Brabec, Radka Kozakova, Klara Rehakova, Josef Hejzlar, Michal Sorf, Jaroslav Vrba
Summary: This study investigates the seasonal succession of plankton communities in freshwater temperate lakes during the winter season. The results show that in warm monomictic lakes, there is a significant increase in phytoplankton chlorophyll a concentrations after the winter solstice and it continues throughout the total circulation and/or unstable inverse stratification period. Solar radiation and water temperature have positive effects on chlorophyll a concentrations, while water age has a negative effect. On the other hand, zooplankton density and ice cover effects are not significant.
Article
Biochemical Research Methods
Tomas Rezanka, Jaromir Lukavsky, Martin Rozmos, Linda Nedbalova, Jan Jansa
Summary: The positional isomers of TAGs containing hypogeic, palmitoleic, and palmitvaccenic acids from three microorganisms were analyzed. Various regioisomers and enantiomers of TAGs containing different combinations of hexadecenoic acids were identified. The dominant TAGs in the AM fungus and containing palmitvaccenic acid were found to bind at the sn-1 position of the glycerol backbone. The covalent adduct chemical ionization method was used to identify TAGs containing different hexadecenoic acids.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Plant Sciences
Lenka Prochazkova, Ryo Matsuzaki, Tomas Rezanka, Linda Nedbalova, Daniel Remias
Summary: In this study, a new species of green snow algae called Chloromonas kaweckae was discovered on the surface of snow in the High Tatra Mountains in Slovakia. This species is able to perform photosynthesis at low temperatures and accumulate polyunsaturated fatty acids. These findings indicate that C. kaweckae has adapted to high light intensities, which is unusual for snow algae.
JOURNAL OF PHYCOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Gergely Erno Lakatos, Karolina Ranglova, Daniela Barcenas-Perez, Tomas Grivalsky, Joao Camara Manoel, Mykola Mylenko, Jose Cheel, Jozsef Nyari, Roland Wirth, Kornel L. Kovacs, Jiri Kopecky, Linda Nedbalova, Jiri Masojidek
Summary: Three cultivation regimes were tested for cold-adapted cultures of Monoraphidium in an outdoor pond: sunlight cultivation, combination of sunlight and supplementary illumination, and nitrogen depletion using both light sources. The highest productivity was achieved when sunlight was combined with supplementary illumination, with a specific growth rate of 0.191 d-1. Nitrogen depletion stress resulted in the highest total fatty acid content of 20.29% DW, with oleic acid accounting for 54% of the total fatty acid content. The cultivation under sunlight in the first trial yielded a significant amount of lutein, 26.39 mg lutein g-1 DW. In the second trial, 236 mLN g-1oTS of methane was generated from the harvested and fermented biomass.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
J. Lukavsky, J. Kopecky, D. Kubac, J. Kviderova, L. Prochazkova, T. Rezanka
Summary: A unicellular alga isolated from snow in Spain was identified as Bracteacoccus bullatus through comparative analysis and it was found that it exhibits sexual reproduction. The alga thrives at a temperature of 21 degrees Celsius and at a light intensity of 160 μmol photons/m²s. It has the potential to be used in the production of vegetable oil with a high content of unsaturated fatty acids.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Geography, Physical
Daniel Remias, Lenka Prochazkova
Summary: Melting glacier surfaces are unique ecosystems for specialized microbes, frequently hosting blooms of microalgae that contribute to the darkening of ice surfaces and accelerating melting rates. The main cause of this phenomenon is a type of algae called Ancylonema. Previous research relied on field-collected samples because these algae were difficult to cultivate. To enhance the understanding of their dominance in melting ice, a strain of Ancylonema alaskanum was established in a laboratory setting, mimicking freezing temperatures and low levels of sunlight. The strain exhibited changes in morphology and pigmentation, indicating the importance of dark phenols for survival in the cryosphere. Comparative studies between the strain and glacial material revealed adaptations of the photosynthetic apparatus to the prevailing conditions. This laboratory strain opens up possibilities for comprehensive omics research.
JOURNAL OF GLACIOLOGY
(2023)
Article
Microbiology
Katerina Nemeckova, Jan Mares, Lenka Prochazkova, Adam Culka, Filip Kosek, Jacek Wierzchos, Linda Nedbalova, Jan Dudak, Veronika Tymlova, Jan Zemlicka, Andreja Kust, Jan Zima, Eva Novakova, Jan Jehlicka
Summary: This study used microscopic, spectroscopic, and molecular analysis to characterize endolithic colonization in gypsum from various sites in Sicily. Cyanobacteria were found to be the dominant microorganisms in these environments, forming differently pigmented zones within the gypsum. The study also revealed differences in the taxonomic composition of endoliths in different gypsum varieties and identified carotenoids as the most common pigments present. These findings provide new insights into the diversity and distribution of phototrophic microorganisms and their pigments in gypsum, highlighting the complex nature of endolithic ecosystems and the effects of gypsum varieties on these communities.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Hirono Suzuki, Alexandre Detain, Youngjin Park, Kiron Viswanath, Rene H. Wijffels, Nathalie Leborgne-Castel, Lenka Prochazkova, Chris J. Hulatt
Summary: Snow algae blooms in melting snowfields are poorly understood in terms of their biology, biogeography, and species diversity. This study investigated eight isolates from red snow in northern Norway using morphology and genetic markers. Six isolates were assigned to known species, while two isolates were potentially new species. Further experiments revealed differences in growth rate and fatty acid profiles among strains, as well as the accumulation of lipids under physiological stress. This study provides new insights into the biodiversity and biogeography of snow algae, as well as their physiological traits and ecological properties.
FEMS MICROBIOLOGY ECOLOGY
(2023)