Article
Ophthalmology
Ricardo Usategui-Martin, Kevin Puertas-Neyra, Nadia Galindo-Cabello, Leticia A. Hernandez-Rodriguez, Fernando Gonzalez-Perez, Jose Carlos Rodriguez-Cabello, Rogelio Gonzalez-Sarmiento, Jose Carlos Pastor, Ivan Fernandez-Bueno
Summary: This study evaluated the neuroprotective capability of the secretome of human bone marrow MSCs and found that it can slow retinal neurodegeneration by activating antioxidant machinery, modulating autophagy, and inhibiting apoptosis and necroptosis. The secretome of bmMSCs may be a potential therapeutic option for treating retinal neurodegenerative diseases.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Xiaowen Mao, Seta Stanbouly, Jacob Holley, Michael Pecaut, James Crapo
Summary: The aim of this study was to investigate the acute oxidative damage in ocular structure and retinal function after exposure to spaceflight, and evaluate the effectiveness of an antioxidant in mitigating spaceflight-induced changes in the retina. Results showed that spaceflight conditions induced retinal oxidative stress, leading to photoreceptor cell damage and impaired retinal function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Yasaman Esmaeili, Zahra Yarjanli, Fatemeh Pakniya, Elham Bidram, Marek J. Los, Mehdi Eshraghi, Daniel J. Klionsky, Saeid Ghavami, Ali Zarrabi
Summary: Protein homeostasis is crucial for cell function, and disruption of the molecular mechanisms involved in this process may contribute to neurodegenerative diseases. Autophagy disruption, oxidative stress, and ER stress are the main molecular mechanisms underlying neurodegeneration, and they have synergistic effects on each other. Therefore, targeting the crosstalk between these pathways may play a critical role in healing neurodegenerative diseases.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Neurosciences
Davide Garzone, Robert P. Finger, Matthias M. Mauschitz, Alexandra Koch, Martin Reuter, Monique M. B. Breteler, N. Ahmad Aziz
Summary: Visual impairment and retinal neurodegeneration are associated with cognitive impairment and brain atrophy. This study found that visual acuity and retinal neurodegeneration are both related to global brain and hippocampal atrophy, partially mediated through the disintegration of intermediate visual tracts. These findings indicate that OCT-derived retinal measures can be used as markers of neurodegeneration and emphasize the importance of transneuronal degeneration in the association between retinal neurodegeneration and brain atrophy.
HUMAN BRAIN MAPPING
(2023)
Article
Neurosciences
Huirong Li, Bo Liu, Lili Lian, Jiajia Zhou, Shengjin Xiang, Yifan Zhai, Yu Chen, Xiaoyin Ma, Wencan Wu, Ling Hou
Summary: The study found that the expression levels of HMOX1 determine whether it is protective or deleterious in the retina. Low levels of HMOX1 before light exposure prevent photoreceptor degeneration, while high levels of HMOX1 directly induce photoreceptor degeneration even without light stress. The high level of HMOX1 is associated with DDIT3 induction and endoplasmic reticulum stress, which can lead to devastating effects, highlighting the importance of carefully considering expression levels in gene therapy approaches involving HMOX1.
MOLECULAR NEURODEGENERATION
(2021)
Article
Biochemistry & Molecular Biology
Elisabetta Catalani, Silvia Bongiorni, Anna Rita Taddei, Marta Mezzetti, Federica Silvestri, Marco Coazzoli, Silvia Zecchini, Matteo Giovarelli, Cristiana Perrotta, Clara De Palma, Emilio Clementi, Marcello Ceci, Giorgio Prantera, Davide Cervia
Summary: The absence of full-length dystrophin leads to structural damages and impaired autophagy in the retina, affecting neuronal survival and visual properties. Reactivation of autophagy can prevent cell death and structural changes, improving the response to light in mutant flies. Overall, functional dystrophin is crucial for synapsis stabilization and proper neuronal survival in the retina.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Hongtao Shen, Jing Li, Tyler Heisler-Taylor, Ryan Makin, Huan Yang, Timur A. Mavlyutov, Bradley Gelfand, Colleen M. Cebulla, Lian-Wang Guo
Summary: The absence of TMEM97 in RPE cells disrupts redox-balancing systems, leading to increased oxidative stress and loss of photoreceptors. TMEM97 KO also affects proteins crucial to mitochondrial and lysosomal stability, as well as impeding autophagy flux.
CELLULAR SIGNALLING
(2021)
Article
Medicine, Research & Experimental
Bo-Yie Chen, Jyh-Cheng Liou, Jia-Lain Wu, Chao-Hsiang Chen, Shih-Liang Yang
Summary: Astragaloside IV showed protective effects against light-induced functional vision deterioration, especially on cone cells, improving visual acuity and contrast sensitivity function in mice.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Cell Biology
Sreesha Sree, Ilmari Parkkinen, Anna Their, Mikko Airavaara, Eija Jokitalo
Summary: The endoplasmic reticulum (ER) in neurons plays a crucial role in maintaining cellular homeostasis and integrity. Different neuronal types exhibit variations in ER morphology and functionality, leading to differences in sensitivity to stress and neurodegenerative changes. The heterogeneity of ER morphology in neurons is proposed to be an important factor in determining their selective susceptibility to neurodegeneration.
Article
Biochemistry & Molecular Biology
Rosario Amato, Alessio Canovai, Alberto Melecchi, Salvatore Pezzino, Roberta Corsaro, Massimo Dal Monte, Dario Rusciano, Paola Bagnoli, Maurizio Cammalleri
Summary: Studies have shown that the combination of lutein and C3G can prevent oxidative stress, inflammation, gliotic and apoptotic responses caused by LD, thus protecting photoreceptor cells more effectively than each component alone. Furthermore, they can improve dysfunctional electroretinogram and enhance rod and cone photoreceptor responses.
Article
Pharmacology & Pharmacy
Hong Xu, Hui Li, Dexiang Liu, Wen Wen, Mei Xu, Jacqueline A. Frank, Jing Chen, Haining Zhu, Nicholas J. Grahame, Jia Luo
Summary: Chronic alcohol abuse can cause a variety of physical and mental disorders by affecting neurotransmitters, neuroinflammation, neurodegeneration, and thiamine levels in the brain. This study using female cHAP mice as a model of chronic voluntary drinking found that alcohol consumption led to anxiety-like behaviors.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Zhiyang Huang, Jiahong Gong, Wen Lin, Zhiyi Feng, Yirou Ma, Yurong Tu, Xiong Cai, Jianhua Liu, Chang Lv, Xinru Lv, Qiuji Wu, Wenjie Lu, Juan Zhao, Yibo Ying, Shengcun Li, Wenfei Ni, Haili Chen
Summary: CAT can inhibit neuronal apoptosis in SCI by reducing endoplasmic reticulum stress and activating the Caspase3/Bax/Bcl-2 pathway, and it has the potential to be a therapeutic drug for SCI.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Aritra Bej, James B. Ames
Summary: Retinal cyclic nucleotide-gated (CNG) ion channels play a crucial role in visual phototransduction. This review discusses the binding of calmodulin to CNGB1 subunits and its impact on channel desensitization and retinal disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Naouel Gharbi, Dagne Roise, Jorunn-Elise Forre, Amanda J. Edson, Helena A. Hushagen, Valentina Tronci, Ann-Kristin Froyset, Kari E. Fladmark
Summary: The redox-sensitive protein DJ-1 is essential in protecting the retina from oxidative stress, with its absence leading to age-dependent degenerative changes. Restoration of DJ-1 expression in Muller cells inhibits the degenerative phenotype, highlighting the key role of Muller cell DJ-1 in protecting the retina from oxidative stress associated with aging.
Article
Biochemistry & Molecular Biology
Wen Wen, Yongchao Wang, Hui Li, Di Hu, Zuohui Zhang, Hong Lin, Jia Luo
Summary: Alcohol exposure has detrimental effects on both developing and mature brain, and it induces endoplasmic reticulum (ER) stress, contributing to alcohol-induced neuronal damages. Mesencephalic astrocyte-derived neurotrophic factor (MANF) is an ER stress-responsive protein that provides neuroprotection. This study shows that alcohol increases MANF expression by inducing ER stress, and exogenous MANF supplementation can alleviate alcohol neurotoxicity.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Ophthalmology
Akiko Hanyuda, Atsushi Goto, Masahiro Nakatochi, Yoichi Sutoh, Akira Narita, Shiori Nakano, Ryoko Katagiri, Kenji Wakai, Naoyuki Takashima, Teruhide Koyama, Kokichi Arisawa, Issei Imoto, Yukihide Momozawa, Kozo Tanno, Atsushi Shimizu, Atsushi Hozawa, Kengo Kinoshita, Taiki Yamaji, Norie Sawada, Masao Iwagami, Kenya Yuki, Kazuo Tsubota, Kazuno Negishi, Keitaro Matsuo, Masayuki Yamamoto, Makoto Sasaki, Shoichiro Tsugane, Motoki Iwasaki
Summary: This study conducted a Mendelian randomization analysis to assess the causal association between genetically predicted glycemic traits and the risk of primary open-angle glaucoma (POAG). The results did not provide strong evidence to support the association between genetically predicted glycemic traits and POAG in the Japanese population.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2023)
Editorial Material
Ophthalmology
Naoko Kato, Toshiki Shimizu, Eisuke Shimizu, Nobuhisa Mizuki, Kazuno Negishi
Editorial Material
Cell Biology
Deokho Lee, Yohei Tomita, Kazuno Negishi, Toshihide Kurihara
NEURAL REGENERATION RESEARCH
(2023)
Article
Neurosciences
Ayano Katagiri, Kazuo Tsubota, Lou Mikuzuki, Shigeru Nakamura, Akira Toyofuku, Takafumi Kato, David A. Bereiter, Koichi Iwata
Summary: Repeated application of diquafosol sodium reduces ocular pain in dry eye disease by suppressing peripheral P2Y2 expression. Diquafosol increases tear volume and decreases eyeblink frequency, suggesting its efficacy in relieving dry eye symptoms.
NEUROSCIENCE RESEARCH
(2023)
Article
Multidisciplinary Sciences
Deokho Lee, Ayaka Nakai, Yukihiro Miwa, Kazuno Negishi, Yohei Tomita, Toshihide Kurihara
Summary: Pathological choroidal neovascularization (CNV) is a major cause of visual impairment in neovascular age-related macular degeneration (AMD), and anti-vascular endothelial growth factor (VEGF) antibodies have been used to suppress CNV. However, some cases show failure of anti-VEGF therapies and the agents may induce ocular atrophy. Activation of peroxisome proliferator-activated receptor alpha (PPAR alpha) with pemafibrate treatment has been suggested as a potential therapeutic target for preventing ocular ischemia. This study aimed to examine the preventive role of pemafibrate in laser-induced pathological CNV.
Article
Medicine, General & Internal
Murat Dogru, Takashi Kojima, Kazunari Higa, Ayako Igarashi, Haruka Kudo, Wolfgang G. K. Mueller-Lierheim, Kazuo Tsubota, Kazuno Negishi
Summary: The study found that a novel high molecular weight hyaluronic acid and Latanoprost eye drop had similar intraocular pressure-lowering effects compared to commercially available Latanoprost eye drops, while also reducing ocular surface inflammation. This suggests that the new eye drop formulation provides comparable IOP lowering effects to the market product, while also reducing ocular surface alterations and inflammatory reactions.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Satoshi Imanishi, Yohei Tomita, Kazuno Negishi, Kazuo Tsubota, Toshihide Kurihara
Summary: Disorders in the development and regulation of blood vessels are involved in various ocular disorders. Understanding the developing choroidal circulation system expands our knowledge of ocular development and supports our understanding of ocular disorders. This review examines studies on regulating the developing choroidal circulation system and discusses its relevance to human diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ophthalmology
Ziyan Ma, Heonuk Jeong, Yajing Yang, Xiaoyan Jiang, Shin-ichi Ikeda, Kazuno Negishi, Toshihide Kurihara, Kazuo Tsubota
Summary: This study aimed to investigate the contralateral effects of monocular experimental myopia and recovery, which could not be explained by local mechanisms. The results showed that complete recovery of the eyes that wore -30 D lenses was observed after 1 week of lens removal. Both eyes in the PLC and NLC groups exhibited a myopic shift and choroidal thinning after lens removal. The eye wearing the -30 D lens had a significant increase in axial length after 3 weeks of lens wear, while the fellow eyes in the PLC and NLC groups continued to increase in axial length and the binocular axial lengths gradually converged.
OPHTHALMIC AND PHYSIOLOGICAL OPTICS
(2023)
Article
Multidisciplinary Sciences
Eisuke Shimizu, Toshiki Ishikawa, Makoto Tanji, Naomichi Agata, Shintaro Nakayama, Yo Nakahara, Ryota Yokoiwa, Shinri Sato, Akiko Hanyuda, Yoko Ogawa, Masatoshi Hirayama, Kazuo Tsubota, Yasunori Sato, Jun Shimazaki, Kazuno Negishi
Summary: This study used the Smart Eye Camera to collect videos of the eye and developed an AI algorithm to estimate tear film breakup time and diagnose dry eye disease (DED) based on the ADES criteria. The accuracy of tear film breakup time estimation was 0.789, and the area under the receiver operating characteristic curve of the AI model was 0.877. The sensitivity and specificity of the AI model for DED diagnosis were 0.778 and 0.857, respectively. A novel AI-based diagnostic model for DED was successfully developed.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Jing Hou, Kiwako Mori, Shin-ichi Ikeda, Heonuk Jeong, Hidemasa Torii, Kazuno Negishi, Toshihide Kurihara, Kazuo Tsubota
Summary: Myopia is becoming more common worldwide, and preventive methods need to be developed. The study found that Ginkgo biloba extracts (GBEs) activate the early growth response 1 (EGR-1) protein, and oral GBEs significantly improved refractive errors and axial elongation in lens-induced myopia mice. Additionally, GBEs improved choroidal blood perfusion and expression of Egr-1 and endothelial nitric oxide synthase (eNOS) in the choroid, suggesting that GBEs may inhibit the progression of myopia by improving choroidal blood perfusion.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, General & Internal
Masahiko Ayaki, Hidemasa Torii, Erisa Yotsukura, Kazuno Negishi
Summary: The aim of this study was to explore the association between myopia and dry eye (DE)-related ocular parameters. Among DE-related parameters, strip meniscometry value and corneal endothelial cell density were correlated with axial length (AL) in women but not in men. Regarding retinal parameters, ganglion cell complex (GCC) thickness and full macular thickness were correlated with AL in women but not in men. These results suggest a relationship between tear production and AL in elderly women and support the hypothesis that there may be a common upstream factor including the parasympathetic nervous system in the association between tear production and AL or DE and myopia.
FRONTIERS IN MEDICINE
(2023)
Article
Multidisciplinary Sciences
Yoko Ozawa, Kazuhiro Ohgami, Koji Sasaki, Kazufumi Hirano, Toshiyuki Sunaya
Summary: This study aimed to assess the long-term safety and effectiveness of intravitreal aflibercept (IVT-AFL) treatment in Japanese patients with neovascular age-related macular degeneration (nAMD). Results showed acceptable tolerability and effectiveness of IVT-AFL treatment, with a low incidence of adverse events and adverse drug reactions. Most vitreo-retinal events occurred within 6 months of treatment initiation, while increased intraocular pressure and cerebral infarction mostly occurred after 6 months of follow-up.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Saki Sakakura, Emi Inagaki, Yuichiro Ochiai, Masatoshi Yamamoto, Naofumi Takai, Taeko Nagata, Kazunari Higa, Yasunori Sato, Hiroshi Toshida, Dogru Murat, Masatoshi Hirayama, Yoko Ogawa, Kazuno Negishi, Shigeto Shimmura
Summary: The tear film stability of a rabbit dry eye model induced by dacryoadenectomy was assessed in this study. The results showed that this model partially captured the phenotypes of human short TBUT-type dry eye, making it a potentially useful animal model for investigating potential drug candidates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Norihiro Nagai, Yasuaki Mushiga, Yoko Ozawa
Summary: In this study, a method called swpPERG was used to assess the visual function of diabetic eyes and found that the signal-to-noise ratios were lower in diabetic eyes. Moreover, this method was also correlated with inner retinal morphological changes, making it useful for detecting subtle visual changes in diabetic eyes.
SCIENTIFIC REPORTS
(2023)
